Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

doi:10.3969/j.issn.1004-1524.2021.12.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2264-2274.DOI: 10.3969/j.issn.1004-1524.2021.12.06

• Animal Science • Previous Articles Next Articles

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

BAI Hao¹(), LI Xiaofan², ZHONG Li², SONG Qianqian², JIANG Yong², ZHANG Yang², WANG Zhixiu², XU Qi², CHANG Guobin¹^,², CHEN Guohong¹^,²^,^*()

1. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2020-08-25 Online:2021-12-25 Published:2022-01-10
Contact: CHEN Guohong

Abstract

Abstract:

This study was conducted to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Liancheng white duck. The contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age, respectively, were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In view of the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at the age of 49-63 days. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at the age of 21 days (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at birth (P<0.05); the expression of Fe-related gene FTH1 showed a significant decrease and then a significant increase with the change of age, which had the lowest expression at the age of 35 days (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 0 and 21 days, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at birth (P<0.05). In addition, there were negative correlations between the expression level of genes and the deposition of mineral elements in liver tissue but not significantly (P>0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of high quality strain of Liancheng white duck; 49-63 days can be used as the reference of the best marketing age.

Key words: Liancheng white duck, mineral elements, gene expression, correlation

CLC Number:

S834

BAI Hao, LI Xiaofan, ZHONG Li, SONG Qianqian, JIANG Yong, ZHANG Yang, WANG Zhixiu, XU Qi, CHANG Guobin, CHEN Guohong. Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2264-2274.

Figures/Tables 10

References 32

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项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.88^**	1
Fe	0.43	0.48	1
Se	-0.57	-0.54	0.01	1
Zn	0.22	0.21	0.22	0.62	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.67^*	1
Fe	0.19	0.01	1
Se	-0.10	0.44	0.08	1
Zn	-0.37	-0.30	0.05	0.33	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.22	1
Fe	0.01	-0.30	1
Se	0.08	-0.30	0.49	1
Zn	-0.13	-0.30	0.27	-0.10	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.84^**	1
Fe	0.68	0.49	1
Se	0.46	0.14	0.66	1
Zn	-0.13	-0.20	0.52	0.04	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.23	1
Fe	-0.51	-0.06	1
Se	-0.43	0.02	0.71^*	1
Zn	0.59	0.08	-0.35	0.10	1

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

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0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.69^*	1
Fe	-0.11	0.11	1
Se	0.12	-0.10	0.42	1
Zn	0.29	0.52	0.78^*	0.44	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.68^*	1
Fe	0.21	0.44	1
Se	0.27	0.20	-0.08	1
Zn	0.02	0.33	0.75^*	0.22	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.35	0.07	1
Se	-0.04	0.03	0.29	1
Zn	0.29	0.45	0.35	0.01	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.21	1
Fe	-0.50	0.62	1
Se	-0.07	0.20	0.52	1
Zn	-0.61	0.01	0.86^*	0.19	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.27	0.56	1
Se	-0.41	-0.17	-0.20	1
Zn	-0.06	0.14	0.63	0.20	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.92^**	1
Fe	-0.10	-0.22	1
Se	0.76^*	0.64^*	-0.30	1
Zn	0.15	0.09	0.61	0.01	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.64^*	1
Fe	0.46	0.43	1
Se	0.95^**	0.32	0.42	1
Zn	0.74^*	0.49	0.79^*	0.70^*	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.16	0.10	1
Se	0.55	0.38	0.79^*	1
Zn	0.74^*	0.71^*	0.18	0.59	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.71^*	1
Fe	0.19	0.33	1
Se	0.65^*	0.78^**	0.57	1
Zn	0.63	0.61	0.24	0.54	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.76^*	1
Fe	-0.54	0.09	1
Se	0.10	0.55	0.07	1
Zn	0.13	-0.50	-0.50	0.50	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.31	1
Fe	0.58	0.38	1
Se	0.45	-0.10	-0.20	1
Zn	-0.50	0.21	-0.10	-0.60	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.99^**	1
Fe	0.53	0.49	1
Se	0.86^**	0.86^**	0.18	1
Zn	0.98^**	0.97^**	0.51	0.88^**	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^**	1
Fe	0.43	-0.50	1
Se	0.05	0.38	-0.10	1
Zn	0.77^*	-0.10	0.72^*	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.98^**	1
Fe	0.02	-0.10	1
Se	0.53	0.52	-0.30	1
Zn	-0.1	0.01	-0.60	0.21	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.85^**	1
Fe	0.56	-0.10	1
Se	-0.50	-0.40	-0.20	1
Zn	0.46	0.04	0.45	0.40	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.90^*	1
Fe	0.96	-0.80	1
Se	-0.90	0.25	-0.90	1
Zn	0.67	-0.70	0.10	0.21	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^*	1
Fe	-0.72	-0.90	1
Se	-0.30	-0.10	0.65	1
Zn	0.14	-0.20	-0.80	-0.10	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.47	1
Fe	0.08	-0.10	1
Se	0.74	-0.80^*	-0.16	1
Zn	0.36	0.53	0.50	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.94	0.35	1
Se	-0.50	-0.40	-0.10	1
Zn	-0.90	0.26	-0.70	0.17	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.72^*	1
Fe	0.28	0.85	1
Se	0.72	-0.70	-0.50	1
Zn	-0.90^*	0.6	0.31	-0.80^*	1

基因 Gene	矿物元素Mineral elements
基因 Gene	Mg	K	Fe	Se	Zn
TRPM6	-0.482	—	—	—	—
TRPM7	-0.601	—	—	—	—
ATP1A1	—	-0.821	—	—	—
ATP1B1	—	-0.770	—	—	—
FTH1	—	—	-0.651	—	—
GPX1	—	—	—	-0.001	—
GPX4	—	—	—	-0.729	—
ATP6	—	—	—		-0.798
ATP8	—	—	—	—	-0.730

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