Analysis of anthocyanin synthesis and related gene expression in blood orange peel under different PE materials shading during fruit coloring period

doi:10.3969/j.issn.1004-1524.2021.10.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (10): 1861-1869.DOI: 10.3969/j.issn.1004-1524.2021.10.09

• Horticultural Science • Previous Articles Next Articles

Analysis of anthocyanin synthesis and related gene expression in blood orange peel under different PE materials shading during fruit coloring period

YANG Haijian(), ZHANG Yungui^*(), ZHOU Xinzhi, HONG Lin, YANG Lei, PENG Fangfang, WANG Wu

Fruit Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China

Received:2020-08-27 Online:2021-10-25 Published:2021-11-02
Contact: ZHANG Yungui

Abstract

Abstract:

To explore the relationship between light and anthocyanin synthesis of blood orange peel,some PE bags with different light transmittance was used to perform shading experiments on the fruit during the ripening period. This experiment determined the dynamic content of total anthocyanins and the dynamic expression of 12 anthocyanin synthesis-related genes in blood orange peels under different shading treatments. The results showed that coloring time, coloring area and coloring degree of the blood orange fruit were hindered by different degrees of shading. The degree of obstruction was related to the light transmittance of the PE bags, but the PE bags shading promoted the increase of the brightness of the blood orange fruit surface. In the natural state,the expression of 8 structural genes GST, ANS, CHS, DFR, F3H, UFGT, PAL, 4CL and two regulatory genes Ruby and MYBF1 showed a rapid increase during the color conversion period, and different degrees of shading hindered them. So these 10 genes were the key genes of blood orange peel anthocyanin synthesis regulated by light. After the comprehensive analysis of the dynamic content of total anthocyanins, the dynamic expression of key genes and the corresponding PE bags light transmittance data, it could be inferred that the UVB ultraviolet light might regulate the synthesis of blood orange peel anthocyanins in the light. According to the dynamic determination results of related genes, it was speculated that the critical period of blood orange peel anthocyanin synthesis appeared on 249 d after flowering. For better appearance quality, it was more appropriate to pick blood oranges on 249 d after flowering. Transparent PE film or other films with higher UV transmittance should be used to protect the fruit of blood orange in winter.

Key words: blood orange, anthocyanin, light, gene expression

CLC Number:

S666

YANG Haijian, ZHANG Yungui, ZHOU Xinzhi, HONG Lin, YANG Lei, PENG Fangfang, WANG Wu. Analysis of anthocyanin synthesis and related gene expression in blood orange peel under different PE materials shading during fruit coloring period[J]. Acta Agriculturae Zhejiangensis, 2021, 33(10): 1861-1869.

Figures/Tables 6

Table 1 Nucleotide sequences of primers in qRT-PCR

基因 Gene	上游引物Forward primer(5'→3')	下游引物Reverse primer (5'→3')
PAL	GATTTGAGACATTTGGAGGA	ATGGATGAAGCTCTCCACTA
CHS	TCTATCGACGGGCATCTTC	TGCCTCGGTTAGGCTTTTC
C4H	CGTGGGAAGGCGAAGCT	TCACAAGCCCCAAGATTGGT
F3H	CCCCTAATTTAAGGCTACCC	GACATCGTTCACGAAAACATAC
UFGT	TCTTCAGCACTCCGCAATC	TCCATCGGATACGTCGTAAG
MYBF1	AGGTGGTGCTGAGTGTCATAATC	ATAACTTGTCATCTCCATCCCG
DFR	GCTGTTCGTGCTACTGTTC	GGCTAAATCGGCTTTCCATA
CHI	AACAAAGTCGCATTTCCTCGGC	GCACGGCGTTCTCCTCCAAGTA
ANS	GGGTGACTGCTAAATGTGTT	CAAGTCCCCTGTGAAGAATA
4CL	CCGAAACGCACCTCATCTG	TGATGCCGAGATTCGACAAA
GST	GCAGCAAAGTATGCAAACC	GTCATTGAAATTGTGTGCTTC
Ruby	GTCGGCCAGAAGAGGAATC	GATCCCAAAGTTCCGCGTC
EF-1α	GGCTAGGTACGATGAAATTG	GTTGTCACCCTCGAAACC

Fig.1 Light transmittance of PE bag

Fig.2 Changes in color of blood orange peel under different shading treatments during the ripening period

Fig.3 Changes in total anthocyanin content of blood orange peel under different shading treatments during the ripening period A, B, C, D, E, and F represented the control, transparent bag treatment, red bag treatment, green bag treatment, blue bag treatment, and purple bag treatment respectively. Different lowercase letters above the bars represented statistically significant (P<0.05) differences among treatments.

Table 2 The skin color of blood orange fruit by different treatments

处理 Treatment	a	b	L
对照 Control	39.47 a	48.31 a	54.57 a
透明袋 Transparent bag	38.88 ab	56.06 ab	60.08 b
红袋 Red bag	35.98 c	67.23 c	68.72 d
绿袋 Green bag	37.64 bc	54.78 a	60.07 b
蓝袋 Blue bag	36.51 c	62.37 bc	65.03 cd
紫袋 Purple bag	37.82 abc	58.52 ab	62.19 bc

Fig.4 Changes in transcription levels of anthocyanin biosynthesis-related genes in the peel of blood oranges during the ripening period under different shading conditions A, B, C, D, E and F represented the control, transparent bag treatment, red bag treatment, green bag treatment, blue bag treatment and purple bag treatment, respectively.

References 22

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Analysis of anthocyanin synthesis and related gene expression in blood orange peel under different PE materials shading during fruit coloring period

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