Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

doi:10.3969/j.issn.1004-1524.2021.12.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2264-2274.DOI: 10.3969/j.issn.1004-1524.2021.12.06

• Animal Science • Previous Articles Next Articles

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

BAI Hao¹(), LI Xiaofan², ZHONG Li², SONG Qianqian², JIANG Yong², ZHANG Yang², WANG Zhixiu², XU Qi², CHANG Guobin¹^,², CHEN Guohong¹^,²^,^*()

1. Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2020-08-25 Online:2021-12-25 Published:2022-01-10
Contact: CHEN Guohong

Abstract

Abstract:

This study was conducted to investigate the depositions of several mineral elements and the mRNA expression levels of mineral-related genes in different tissues of Liancheng white duck. The contents of Mg, K, Fe, Zn and Se in breast muscle, thigh muscle, liver, skin and tibia at 0, 21, 35, 49 and 63 days of age, respectively, were measured by atomic fluorescence spectrophotometer. The mRNA expression levels of mineral-related genes were detected by qRT-PCR. The results revealed that the depositions of Mg and K were basically the same in all tissues, showing a significant positive correlation (P<0.05). In view of the breast muscle, thigh muscle and liver, the contents of almost all the mineral elements reached the peak value (P<0.05) at the age of 49-63 days. The expression levels of Mg-related genes TRPM6 and TRPM7 were the highest at the age of 21 days (P<0.05); the expression levels of K-related genes ATP1A1 and ATP1B1 were the highest at birth (P<0.05); the expression of Fe-related gene FTH1 showed a significant decrease and then a significant increase with the change of age, which had the lowest expression at the age of 35 days (P<0.05); the expression levels of Zn-related genes ATP6 and ATP8 were the highest at 0 and 21 days, respectively (P<0.05); the expression levels of Se-related genes GPX1 and GPX4 were the highest at birth (P<0.05). In addition, there were negative correlations between the expression level of genes and the deposition of mineral elements in liver tissue but not significantly (P>0.05). Taken together, Mg and K could be used as joint indicators for the precise breeding of high quality strain of Liancheng white duck; 49-63 days can be used as the reference of the best marketing age.

Key words: Liancheng white duck, mineral elements, gene expression, correlation

CLC Number:

S834

BAI Hao, LI Xiaofan, ZHONG Li, SONG Qianqian, JIANG Yong, ZHANG Yang, WANG Zhixiu, XU Qi, CHANG Guobin, CHEN Guohong. Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2264-2274.

Figures/Tables 10

References 32

[1]	耿华田. 镁元素与人体健康[J]. 中学生物学, 2005, 21(6): 7-8.
	GENG H T. Magnesium and human body health[J]. Middle School Biology, 2005, 21(6): 7-8.(in Chinese)
[2]	吴茂江. 钾与人体健康[J]. 微量元素与健康研究, 2011, 28(6): 61-62.
	WU M J. Potassium and human health[J]. Studies of Trace Elements and Health, 2011, 28(6): 61-62.(in Chinese with English abstract)
[3]	蔡洁, 张惠迪, 杨丽琛. 人体铁生理需要量研究进展[J]. 卫生研究, 2018, 47(1): 170-172.
	CAI J, ZHANG H D, YANG L C. Research progress in physiological iron requirement of human body[J]. Journal of Hygiene Research, 2018, 47(1): 170-172.(in Chinese)
[4]	SCOTT M E, KOSKI K G. Zinc deficiency impairs immune responses against parasitic nematode infections at intestinal and systemic sites[J]. The Journal of Nutrition, 2000, 130(5): 1412S-1420S. DOI URL
[5]	邵黎雄, 陆建梅, 姜雪峰. 硒,人类不可或缺的元素[J]. 自然杂志, 2019, 41(6): 453-459.
	SHAO L X, LU J M, JIANG X F. Selenium, an indispensable element for humanity[J]. Chinese Journal of Nature, 2019, 41(6): 453-459.(in Chinese with English abstract)
[6]	侯水生, 刘灵芝. 2019年水禽产业现状、未来发展趋势与建议[J]. 中国畜牧杂志, 2020, 56(3): 130-135.
	HOU S S, LIU L Z. Current situation, future development trend and suggestions of waterfowl industry in 2019[J]. Chinese Journal of Animal Science, 2020, 56(3): 130-135.(in Chinese)
[7]	张厉, 杨春喜, 贺石生. TRPM家族对细胞钙/镁平衡调控的研究进展[J]. 基础医学与临床, 2013, 33(3): 370-373.
	ZHANG L, YANG C X, HE S S. Recent advances in the study of regulation effect of TRPM family in cellular calcium/magnesium homeostasis[J]. Basic & Clinical Medicine, 2013, 33(3): 370-373.(in Chinese with English abstract)
[8]	GLORIOSO N, HERRERA V L M, BAGAMASBAD P, et al. Association of ATP1A1 and dear single-nucleotide polymorphism haplotypes with essential hypertension: sex-specific and haplotype-specific effects[J]. Circulation Research, 2007, 100(10): 1522-1529. DOI URL
[9]	EILERT E, HOLLENBERG C P, PIONTEK M, et al. The use of highly expressed FTH1 as carrier protein for cytosolic targeting in Hansenula polymorpha[J]. Journal of Biotechnology, 2012, 159(3): 172-176. DOI URL
[10]	KVÍCALA J. Selenium and the organism[J]. Casopís Lékar Ceských, 1999, 138(4): 99.
[11]	孙润广. F₁F₀-ATP酶的超分子结构和功能的研究[J]. 陕西师范大学学报(自然科学版), 2003, 31(1): 70-75.
	SUN R G. Structure and function of supramolecular complex of F₁F₀-ATP synthase[J]. Journal of Shaanxi Normal University (Natural Science Edition), 2003, 31(1): 70-75.(in Chinese with English abstract)
[12]	张玉芹, 程剑侠, 王照宇, 等. Zn²⁺增强非洲爪蟾卵母细胞ATP-激活电流[J]. 基础医学与临床, 2008, 28(1): 18-21.
	ZHANG Y Q, CHENG J X, WANG Z Y, et al. Augmentation of ATP-activated currents by Zn²⁺ in Xenopus oocytes[J]. Basic & Clinical Medicine, 2008, 28(1): 18-21.(in Chinese with English abstract)
[13]	吴春敏, 夏静, 李强, 等. 微波消解-电感耦合等离子体质谱法同时测定肉及肉制品中7种痕量元素[J]. 肉类研究, 2015, 29(3): 27-29.
	WU C M, XIA J, LI Q, et al. Simultaneous determination of 7 trace elements in meat and meat products by inductively coupled plasma mass spectrometry(ICP-MS) with microwave digestion[J]. Meat Research, 2015, 29(3): 27-29.(in Chinese with English abstract)
[14]	许敏伟, 陈靓, 赵演. 微波消解-火焰原子吸收法检测肉制品中的锌元素[J]. 食品安全质量检测学报, 2013, 4(4): 1161-1164.
	XU M W, CHEN L, ZHAO Y. Detection of zinc in meat products by microwave digestion-flame atomic absorption spectrometry[J]. Journal of Food Safety & Quality, 2013, 4(4): 1161-1164.(in Chinese with English abstract)
[15]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001, 25(4): 402-408. DOI URL
[16]	李欣雨, 王安. 天门冬氨酸镁添加水平对肉仔鸡生长性能及肉品质的影响[J]. 饲料工业, 2009, 30(14): 42-44.
	LI X Y, WANG A. Effect of magnesium aspartate on growth performance and meat quality of broilers[J]. Feed Industry, 2009, 30(14): 42-44.(in Chinese)
[17]	丁斌鹰, 侯永清, 王聪. 复合肉质改进剂对肉鸭肉品质的影响[J]. 黑龙江畜牧兽医, 2008 (9): 109-110.
	DING B Y, HOU Y Q, WANG C. Effect of compound meat quality improver on duck meat quality[J]. Heilongjiang Animal Science and Veterinary Medicine, 2008(9): 109-110.(in Chinese)
[18]	姚友平, 冯汉斌, 赵丽萍, 等. 细胞内外钾分布与人体健康的关系[J]. 公共卫生与预防医学, 2006, 17(1): 57-59.
	YAO Y P, FENG H B, ZHAO L P, et al. The relationship between the distribution of intracellular and extracellular potassium and human health[J]. Journal of Public Health and Preventive Medicine, 2006, 17(1): 57-59.(in Chinese)
[19]	金恒, 邱光忠, 钟云平, 等. 饲养方式对宁都三黄鸡肌肉品质及矿物元素含量的影响[J]. 江西农业学报, 2019, 31(11): 122-126.
	JIN H, QIU G Z, ZHONG Y P, et al. Effects of feeding methods on muscle quality and mineral element content of Ningdu three-yellow chicken[J]. Acta Agriculturae Jiangxi, 2019, 31(11): 122-126.(in Chinese with English abstract)
[20]	TANG Z G, WEN C, WANG L C, et al. Effects of zinc-bearing clinoptilolite on growth performance, cecal microflora and intestinal mucosal function of broiler chickens[J]. Animal Feed Science and Technology, 2014, 189: 98-106. DOI URL
[21]	陈长兰, 郇丰宁, 孟雪莲, 等. 硒对人体的作用机理及科学补硒方法[J]. 辽宁大学学报(自然科学版), 2016, 43(2): 155-168.
	CHEN C L, HUAN F N, MENG X L, et al. The effects of selenium on human health and diseases and the scientifically supplementation of selenium to human bodies[J]. Journal of Liaoning University (Natural Sciences Edition), 2016, 43(2): 155-168.(in Chinese with English abstract)
[22]	ROTRUCK J T, POPE A L, GANTHER H E, et al. Selenium: biochemical role as a component of glutathione peroxidase[J]. Science, 1973, 179(4073): 588-590. DOI URL
[23]	郭建凤, 武英, 刘俊珍, 等. 矿物元素镁对猪肉品质的影响[J]. 吉林农业科学, 2007, 32(5): 44-45.
	GUO J F, WU Y, LIU J Z, et al. Effect of magnesium on pork quality[J]. Journal of Jilin Agricultural Sciences, 2007, 32(5): 44-45. (in Chinese)
[24]	李亮, 何永涛, 丛玉艳. 有机镁对肉仔鸡机体组织抗氧化机能及肉品质的影响[J]. 上海畜牧兽医通讯, 2005(3): 26-27.
	LI L, HE Y T, CONG Y Y. Effects of organic magnesium on antioxidant function and meat quality of broilers[J]. Shanghai Journal of Animal Husbandry and Veterinary Medicine, 2005(3): 26-27.(in Chinese)
[25]	杭柏林, 胡建和, 刘保国, 等. 矿物元素对猪肉品质的影响[J]. 河南科技学院学报(自然科学版), 2008, 36(1): 70-72.
	HANG B L, HU J H, LIU B G, et al. Effect of mineral element on porcine meat quality[J]. Journal of Henan Institute of Science and Technology (Natural Sciences Edition), 2008, 36(1): 70-72.(in Chinese with English abstract)
[26]	滕飞翔, 凌存保. 成年SD大鼠肾脏中6种镁离子转运体的表达[J]. 中国老年保健医学, 2013, 11(3): 16-17.
	TENG F X, LING C B. The expression of six Mg²⁺ transporters in adult SD rat kidney[J]. Chinese Journal of Geriatric Care, 2013, 11(3): 16-17.(in Chinese with English abstract)
[27]	VOETS T, NILIUS B, HOEFS S, et al. TRPM6 forms the Mg²⁺ influx channel involved in intestinal and renal Mg²⁺ absorption[J]. Journal of Biological Chemistry, 2004, 279(1): 19-25. DOI URL
[28]	EL-MALLAKH R S, WYATT R J. The Na, K-ATPase hypojournal for bipolar illness[J]. Biological Psychiatry, 1995, 37(4): 235-244. DOI URL
[29]	HE X Y, CAI J H, LIU B, et al. Cellular magnetic resonance imaging contrast generated by the ferritin heavy chain genetic reporter under the control of a Tet-On switch[J]. Stem Cell Research & Therapy, 2015, 6: 207.
[30]	韩军花, 杨月欣, 何梅, 等. 锌对红细胞膜ATP酶活性影响的研究[J]. 卫生研究, 2001, 30(1): 47-49.
	HAN J H, YANG Y X, HE M, et al. Effect of zinc on the activities of ATPase of erythrocyte membrane[J]. Journal of Hygiene Research, 2001, 30(1): 47-49.(in Chinese with English abstract)
[31]	BERRY M J, LARSEN P R. Molecular cloning of the selenocysteine-containing enzyme type I iodothyronine deiodinase[J]. The American Journal of Clinical Nutrition, 1993, 57(2): 249S-255S. DOI URL
[32]	李黛淋. 日粮硒与维生素E缺乏对雏鸡胰腺中硒蛋白基因表达的影响[D]. 雅安: 四川农业大学, 2011.
	LI D L. The effect of dietary selenium and Vitamin E deficiency on selenoprotein genes expression in pancreas of broiler[D]. Ya’an: Sichuan Agricultural University, 2011. (in Chinese with English abstract)

项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

项目Item	0~7 d	8~21 d	22~42 d	43~63 d
原料Ingredient
玉米Corn/%	10.32	10.63	47.18	21.27
次粉Wheat middling/%	15.41	15.00	6.89	20.00
麦麸Wheat bran/%	-	-	20.00	30.01
米粉Rice noodles/%	35.21	34.99	-	10.00
米糠Rice bran/%	15.81	15.00	3.00	5.00
花生粉Peanut meal/%	-	-	3.00	2.37
玉米蛋白粉Corn gluten meal/%	-	-	5.00	-
豆粕Soybean meal/%	12.63	13.70	5.94	2.50
核苷酸渣Nucleotide slag/%	2.00	2.00	-	-
石灰石粉Limestone powder/%	1.52	1.58	1.90	1.96
磷酸氢钙Calcium hydrogen phosphate/%	1.10	1.10	1.01	0.84
复合预混料Compound premix^a/%	6.00	6.00	6.00	6.00
配方营养成分Formulated nutrient profile
粗蛋白Crude protein/(g·kg^-1)	185.00	190.00	170.00	170.00
粗脂肪Crude fat/(g·kg^-1)	20.00	30.00	35.00	35.00
粗纤维Crude fiber/(g·kg^-1)	60.00	50.00	70.00	70.00
粗灰分Crude ash/(g·kg^-1)	90.00	80.00	100.00	100.00
钙Calcium/(g·kg^-1)	10.00	10.00	10.00	10.00
磷Phosphorus/(g·kg^-1)	5.00	5.50	4.50	4.50
氯化钠Sodium chloride/(g·kg^-1)	6.00	6.00	6.00	6.00
蛋氨酸Methionine/(g·kg^-1)	4.00	4.00	2.80	2.80
水分Moisture/(g·kg^-1)	140.00	130.00	130.00	130.00

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

基因 Gene	引物序列(5'-3') Sequence of primer (5'-3')	产物长度 Product length/bp	退火温度 Tm/℃
TRPM6	F:TTGCAAGGTGTTGGGGAAAAC	199	60
	R:GCCTTTCCATTGTGCAGTCG
TRPM7	F:TGATTGATGTGGGGCTGGTT	124	60
	R:GCACCAGTGTCAATCCGACT
ATP1A1	F:TGAGCCTACTGCAACATCCG	168	60
	R:GCAGTAGTCAAACCCCGACT
ATP1B1	F:TGTGCTCCCAAGAGAGACGA	112	60
	R:GAAGCTTGCCGTAGTAGGGG
FTH1	F:AATGGGCTGACTGCAATGGA	109	60
	R:CACACAGGTGTGGGTCGTT
GPX1	F:ACTTCCTGCAGCTCAACGAG	103	60
	R:TTGGTGGCATTCTCCTGGTG
GPX4	F:GACAACGCGCAGATTAAGGC	152	60
	R:TTTATGGCATTGCCCAGGGT
ATP6	F:TTGGCATCCCCCTGATCCTA	167	60
	R:GGCTCATTTGTGGCCGTTTT
ATP8	F:CCTGACTAACCCTCGCACTC	114	60
	R:ATGGTCAGGCTCATGGTGTG
β-actin	F:GTGCTATGTCGCCCTGGATT	171	60
	R:CCACAGGACTCCATACCCAAG

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.88^**	1
Fe	0.43	0.48	1
Se	-0.57	-0.54	0.01	1
Zn	0.22	0.21	0.22	0.62	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.67^*	1
Fe	0.19	0.01	1
Se	-0.10	0.44	0.08	1
Zn	-0.37	-0.30	0.05	0.33	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.22	1
Fe	0.01	-0.30	1
Se	0.08	-0.30	0.49	1
Zn	-0.13	-0.30	0.27	-0.10	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.84^**	1
Fe	0.68	0.49	1
Se	0.46	0.14	0.66	1
Zn	-0.13	-0.20	0.52	0.04	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.23	1
Fe	-0.51	-0.06	1
Se	-0.43	0.02	0.71^*	1
Zn	0.59	0.08	-0.35	0.10	1

Study on depositions of mineral elements and expression levels of key genes in different tissues of Liancheng white ducks

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 32

Related Articles 15

Recommended Articles

Metrics

Comments

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.69^*	1
Fe	-0.11	0.11	1
Se	0.12	-0.10	0.42	1
Zn	0.29	0.52	0.78^*	0.44	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.68^*	1
Fe	0.21	0.44	1
Se	0.27	0.20	-0.08	1
Zn	0.02	0.33	0.75^*	0.22	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.35	0.07	1
Se	-0.04	0.03	0.29	1
Zn	0.29	0.45	0.35	0.01	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.21	1
Fe	-0.50	0.62	1
Se	-0.07	0.20	0.52	1
Zn	-0.61	0.01	0.86^*	0.19	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.79^**	1
Fe	0.27	0.56	1
Se	-0.41	-0.17	-0.20	1
Zn	-0.06	0.14	0.63	0.20	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.92^**	1
Fe	-0.10	-0.22	1
Se	0.76^*	0.64^*	-0.30	1
Zn	0.15	0.09	0.61	0.01	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.64^*	1
Fe	0.46	0.43	1
Se	0.95^**	0.32	0.42	1
Zn	0.74^*	0.49	0.79^*	0.70^*	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.16	0.10	1
Se	0.55	0.38	0.79^*	1
Zn	0.74^*	0.71^*	0.18	0.59	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.71^*	1
Fe	0.19	0.33	1
Se	0.65^*	0.78^**	0.57	1
Zn	0.63	0.61	0.24	0.54	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.76^*	1
Fe	-0.54	0.09	1
Se	0.10	0.55	0.07	1
Zn	0.13	-0.50	-0.50	0.50	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.31	1
Fe	0.58	0.38	1
Se	0.45	-0.10	-0.20	1
Zn	-0.50	0.21	-0.10	-0.60	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.99^**	1
Fe	0.53	0.49	1
Se	0.86^**	0.86^**	0.18	1
Zn	0.98^**	0.97^**	0.51	0.88^**	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^**	1
Fe	0.43	-0.50	1
Se	0.05	0.38	-0.10	1
Zn	0.77^*	-0.10	0.72^*	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.98^**	1
Fe	0.02	-0.10	1
Se	0.53	0.52	-0.30	1
Zn	-0.1	0.01	-0.60	0.21	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.85^**	1
Fe	0.56	-0.10	1
Se	-0.50	-0.40	-0.20	1
Zn	0.46	0.04	0.45	0.40	1

0 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.90^*	1
Fe	0.96	-0.80	1
Se	-0.90	0.25	-0.90	1
Zn	0.67	-0.70	0.10	0.21	1
21 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.93^*	1
Fe	-0.72	-0.90	1
Se	-0.30	-0.10	0.65	1
Zn	0.14	-0.20	-0.80	-0.10	1
35 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.47	1
Fe	0.08	-0.10	1
Se	0.74	-0.80^*	-0.16	1
Zn	0.36	0.53	0.50	-0.30	1
49 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.36	1
Fe	0.94	0.35	1
Se	-0.50	-0.40	-0.10	1
Zn	-0.90	0.26	-0.70	0.17	1
63 d	Mg	K	Fe	Se	Zn
Mg	1
K	0.72^*	1
Fe	0.28	0.85	1
Se	0.72	-0.70	-0.50	1
Zn	-0.90^*	0.6	0.31	-0.80^*	1

基因 Gene	矿物元素Mineral elements
基因 Gene	Mg	K	Fe	Se	Zn
TRPM6	-0.482	—	—	—	—
TRPM7	-0.601	—	—	—	—
ATP1A1	—	-0.821	—	—	—
ATP1B1	—	-0.770	—	—	—
FTH1	—	—	-0.651	—	—
GPX1	—	—	—	-0.001	—
GPX4	—	—	—	-0.729	—
ATP6	—	—	—		-0.798
ATP8	—	—	—	—	-0.730

[1]	ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.
[2]	YAN Fulin, LANG Yunhu, JIAN Yingquan, CHEN Xiongfei, WEI Wei, WANG Zhiwei, AN Jiangyong, REN Deqiang, DING Ning, WEI Shenghua. Response of yield and quality of Radix Ardisia to soil physiochemical properties [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1766-1775.
[3]	DONG Lili, XU Zhihao, YAN Canlong, FAN Xiaoping, JIN Zelan, WANG Zhonghua. Molecular identification and genetic relationship of different breeding populations in Fritillaria thunbergii based on phenotype and molecular markers [J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1719-1730.
[4]	HE Changxi, ZHENG Jianbo, MA Jianbo, JIA Yongyi, LIU Shili, JIANG Wenping, CHI Meili, CHENG Shun, LI Fei. Cloning and expression analysis of Runx2b in Culter alburnus [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1024-1031.
[5]	LI Yaping, JIN Fulai, HUANG Zonggui, ZHANG Tao, DUAN Xiaojing, JIANG Wu, TAO Zhengming, CHEN Jiadong. Identification and expression pattern analysis of glycoside hydrolase GH3 gene family in Dendrobium officinale [J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 790-799.
[6]	PENG Jiacheng, WU Yue, XU Jiehao, XIA Meiwen, QI Tianpeng, XU Haisheng. Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 247-253.
[7]	LIU Xiaolin, SUN Tingting, YANG Jie, HE Hengbin. Cloning and expression analysis of FLS gene of flavonol synthetase in Lilium auratum and L.speciosum var. gloriosoides [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 344-357.
[8]	WANG Xueyan, XIE Lifen, ZHANG Ting, FANG Pingping, WEI Jing, LI Chaosen, LIU Huiqin, XIANG Xiaomin, ZHAO Dongfeng, GUO Qinwei. Selection of delicious and spicy chili pepper varieties and correlation analysis [J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2490-2500.
[9]	ZHAO Lingji, LIAO Xiangjiao, LIU Dechun, HU Wei, KUANG Liuqing, SONG Jie, YI Mingliang, LIU Yong, YANG Li. Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis [J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2510-2520.
[10]	FU Hongfei, GUO Saisai, ZHENG Jirong. Effects of compound substrate with residues of Solanaceous vegetables on cucumber seedling [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1805-1813.
[11]	XU Yue, WU Xiaomeng, WANG Guoqing, ZOU Yunding, BI Shoudon. Analysis of relationship between number of Ricanidae in tea plantations and biochemical substances in tea leaves [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1834-1843.
[12]	WU Jiao, GONG Chengyu, CHEN Chaoqun, CHEN Hongxu, LIU Junhong, TANG Wenjing, CHU Yuanqi, YANG Wenlong, ZHANG Yao, GONG Ronggao. Ecological response of organic acid metabolism of Huangguogan fruits at different altitudes [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 853-861.
[13]	XU Yang, REN Yilin, WANG Haojie, HUANG Qiuhang, XING Boyuan, CAO Hongliang. Comprehensive evaluation of rape straw biochar as slow-release carrier under different preparation conditions [J]. Acta Agriculturae Zhejiangensis, 2023, 35(4): 893-902.
[14]	LOU Qianqi, LIANG Yan. Quality analysis of five kinds of tomato germplasm resources with different fruit colors [J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 582-589.
[15]	ZHANG Hongmei, WANG Baojun, SHEN Yaqiang, CHENG Wangda. Response of yield and quality of high-quality japonica rice with different grain shapes to regulation of sowing date in northern Zhejiang, China [J]. Acta Agriculturae Zhejiangensis, 2023, 35(12): 2751-2762.