Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis

doi:10.3969/j.issn.1004-1524.20231338

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (11): 2510-2520.DOI: 10.3969/j.issn.1004-1524.20231338

• Horticultural Science • Previous Articles Next Articles

Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis

ZHAO Lingji¹(), LIAO Xiangjiao¹, LIU Dechun¹, HU Wei¹, KUANG Liuqing¹, SONG Jie¹, YI Mingliang², LIU Yong¹^,^*(), YANG Li¹^,^*()

1. College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China
2. Ji’an Jinggang Pomelo Development Service Center, Ji’an 343099, Jiangxi, China

Received:2023-11-27 Online:2024-11-25 Published:2024-11-27

Abstract

Abstract:

This study mainly clarified the variation rule of organic acids in the fruit of Taoxi pomelo which is a local characteristic variety in Jiangxi Province during the room temperature storage period, the causes of organic acid changes in Taoxi pomelo during storage were discussed. The study took the fruits of Taoxi pomelo during the storage period as materials, and applied high performance liquid chromatography (HPLC) to determine the components and contents of organic acids in the fruits stored from 0 d to 150 d after picking, and analyzed the changes in the expression of citric acid synthesis, degradation and transport-related genes by real-time fluorescence quantitative PCR (qRT-PCR). The results showed that it was found that the total organic acid content of Taoxi pomelo fruits was higher than that of storage 0 d during most of the storage period, while the sugar-acid ratio was lower than that of storage 0 d during the whole storage period, and there existed the phenomenon of fruit “returning to acidity”, which may be the main reason for the decline in the quality of Taoxi pomelo fruits in post-harvest storage. The citric acid content of Taoxi pomelo accounted for 78.51%-80.32% of the total organic acid content, and the trends of both changes were consistent. The citric acid content fluctuated greatly at the beginning of storage, reached the maximum value at 40 d after storage. The qRT-PCR analysis of genes related to citric acid synthesis (CmCS, CmPEPC), degradation (CmACL, CmAco3, CmNAD-IDH, CmGS, CmGAD, and CmGABA), and transport (CmCHX, CmDIC, CmVHA, and CmVHP) revealed that the citric acid synthesis genes PEPC1/2 and CS1, the vesicular H⁺-ATPase proton pump gene CmVHA-c4 and the vesicular H⁺-pyrophosphatase proton pump gene CmVHP2 showed broadly similar trends with citric acid accumulation, whereas the citric acid degrading gene GAD5 and the cation/H⁺ exchanging gene CmCHX showed opposite trends with citric acid accumulation, which might be related to the accumulation of citric acid during the early stage of Taoxi pomelo storage. Therefore, the increase in organic acid content and the decrease in sugar-acid ratio during storage of Taoxi pomelo caused the phenomenon of fruit “returning to acidity”, and citric acid plays an important role in this, and its accumulation in the early stage of storage is mainly related to the citric acid synthesis-related genes CmPEPC1/2 and CmCS1, the degradation-related gene CmGAD5, as well as the transporter-related genes CmCHX, CmVHA-c4, and CmVHP2.

Key words: Taoxi pomelo, fruit storage, citric acid, gene expression

CLC Number:

S666

ZHAO Lingji, LIAO Xiangjiao, LIU Dechun, HU Wei, KUANG Liuqing, SONG Jie, YI Mingliang, LIU Yong, YANG Li. Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis[J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2510-2520.

Figures/Tables 6

Fig.1 Dynamic changes of total organic acid content in Taoxi pomelo during storage Different lowercase letters indicate the dynamic differences in total organic acid content throughout the storage period (P<0.05). The same as below.

Fig.2 Dynamic changes of sugar-acid ratio in Taoxi pomelo during storage

Fig.3 Changes of organic acid components in Taoxi pomelo fruit during storage

Fig.4 Expression levels of genes related to citric acid synthesis in Taoxi pomelo fruit during storage

Fig.5 Expression levels of genes related to citric acid degradation in Taoxi pomelo fruit during storage

Fig.6 Expression levels of genes related citric acid transport in Taoxi pomelo fruit during storage

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Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis

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