Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

doi:10.3969/j.issn.1004-1524.20221657

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (11): 2533-2542.DOI: 10.3969/j.issn.1004-1524.20221657

• Animal Science • Previous Articles Next Articles

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

LI Hongyi(), ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao^*()

Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512000, Guangdong, China

Received:2022-11-19 Online:2023-11-25 Published:2023-12-04

Abstract

Abstract:

To study the effect of calcium and phosphorus levels in diet on growth performance and liver metabolism related genes in Magang geese, a total of forty eight Magang geese of 50-day-old were randomly divided into three groups, with male and female in half. Each group had two replicates with eight geese per replicate, every two geese were kept in one cage. On the basis of the same diet, 0%, 0.15% and 0.3% calcium hydrogen phosphate were added respectively, each group was pre-fed for five days and fed for twenty-eight days, during which the daily feed intake was recorded, fecal samples and blood samples were collected regularly, and the content of calcium and phosphorus in geese samples were determined. At the end of the experiment, eight Magang geese were randomly selected from each group for slaughter and the slaughter performance and phosphorylase activity were measured. The liver tissue of each geese were collected for transcriptome sequencing, fluorescence quantitative PCR and enzyme activity detection. The results showed that the feed intake of control group was significantly higher than that of the other two groups (P<0.05). The average daily gain and carcass traits were significantly higher than that of 0.15% level group, with no significant difference from 0.3% level group (P >0.05). In addition, there was no significant difference in the content of calcium and phosphorus in blood among the three groups (P>0.05), but the content of fecal calcium increased with the increase of calcium and phosphorus level (P<0.05). Transcriptome sequencing results showed that there was less difference between control group and 0.15% level group, but more significant difference between control group and 0.3% level group. And most of the differently expressed genes were enriched in sugar metabolism pathway, which was consistent with the quantitative PCR results. Also the activity of phosphorylases, the glucose metabolism-related enzyme, decreased with the increase of calcium and phosphorus levels (P<0.05). In a word, the level of calcium and phosphorus in the diet will affect the food intake of Magang geese, and it will also affect the sugar and lipid metabolism in the liver.

Key words: Magang goose, calcium and phosphorus level, growth performance, gene expression, metabolism

CLC Number:

S835

LI Hongyi, ZHOU Runsheng, LIANG Xiaoling, ZHANG Chuyue, LYU Qixin, YANG Changhua, ZHANG Mao. Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2533-2542.

Figures/Tables 10

References 21

[1]	WANG Y B, WANG W W, ZHANG S, et al. Recommended levels of calcium and non-phytate phosphorus for yellow-feathered broilers (finisher phase)[J]. Animal Bioscience, 2022, 35(12): 1940-1947.
[2]	KOP-BOZBAY C, AKDAG A, ATAN H L, et al. Body weight of young broilers fed with declining calcium and phosphorus contents during the starter period is irresponsive to changes in the skeleton[J]. Journal of Animal Physiology and Animal Nutrition, 2021, 105(4): 747-756.
[3]	HAN J C, WANG X N, WU L H, et al. Dietary calcium levels regulate calcium transporter gene expression levels in the small intestine of broiler chickens[J]. British Poultry Science, 2022, 63(2): 202-210.
[4]	李园园, 习雪勇, 张源生, 等. 低聚糖、酵母钙对霍尔多巴吉鹅的生长性能、体尺指标、屠宰性能和肌肉品质的影响[J]. 饲料工业, 2021, 42(23): 31-36.
	LI Y Y, Ⅺ X Y, ZHANG Y S, et al. Effects of oligosaccharides and yeast calcium on growth performance, body size index, slaughter performance and muscle quality of haldobuji geese[J]. Feed Industry, 2021, 42(23): 31-36. (in Chinese with English abstract)
[5]	ALAGAWANY M, ABD EL-HACK M E, ASHOUR E A, et al. Consequences of varying dietary calcium and phosphorus levels on lipid profile, antioxidant and immunity parameters of growing Egyptian geese[J]. Italian Journal of Animal Science, 2020, 19(1): 1490-1497.
[6]	ALAGAWANY M, ALI ASHOUR E, EL-KHOLY M S, et al. Effect of dietary calcium and phosphorus levels on growth, carcass characteristics and liver and kidney functions of growing Egyptian geese[J]. Poultry Science, 2021, 100(8): 101244.
[7]	宿国强. 不同钙和蛋白质水平饲粮对雏鹅生长性能及肠、肾结构与功能的影响研究[D]. 扬州: 扬州大学, 2022: 94.
	SU G Q. Effects of different calcium and protein levels on growth performance, intestinal and renal structure and function of goslings[D]. Yangzhou: Yangzhou University, 2022: 94. (in Chinese with English abstract)
[8]	奚雨萌, 闫俊书, 应诗家, 等. 高蛋白质高钙饲粮对雏鹅内脏型痛风发生、肾脏功能及肠道微生物区系的影响[J]. 动物营养学报, 2019, 31(2): 612-621.
	Ⅺ Y M, YAN J S, YING S J, et al. Effects of high protein and calcium diets on visceral gout development, kidney function and intestinal microbial community of goslings[J]. Chinese Journal of Animal Nutrition, 2019, 31(2): 612-621. (in Chinese with English abstract)
[9]	REYER H, OSTER M, PONSUKSILI S, et al. Transcriptional responses in jejunum of two layer chicken strains following variations in dietary calcium and phosphorus levels[J]. BMC Genomics, 2021, 22(1): 485.
[10]	KIM D, LANGMEAD B, SALZBERG S L. HISAT: a fast spliced aligner with low memory requirements[J]. Nature Methods, 2015, 12(4): 357-360.
[11]	LI B, DEWEY C N. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome[J]. BMC Bioinformatics, 2011, 12: 323.
[12]	LOVE M I, HUBER W, ANDERS S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J]. Genome Biology, 2014, 15(12): 550.
[13]	孙巍, 贾禄, 孙会, 等. 不同钙水平日粮对鹅肉品质的影响[J]. 饲料工业, 2010, 31(9): 16-18.
	SUN W, JIA L, SUN H, et al. Effects of different calcium levels on goose meat quality[J]. Feed Industry, 2010, 31(9): 16-18. (in Chinese)
[14]	罗旭, 张爱忠, 姜宁, 等. 日粮纤维水平对不同生长阶段籽鹅屠宰性能和血液生化指标的影响[J]. 黑龙江畜牧兽医, 2017(21): 144-148.
	LUO X, ZHANG A Z, JIANG N, et al. Effects of dietary fiber level on slaughter performance and blood biochemical indexes of goose at different growth stages[J]. Heilongjiang Animal Science and Veterinary Medicine, 2017(21): 144-148. (in Chinese)
[15]	王宗伟, 牟晓玲, 杨国伟, 等. 日粮营养水平对肉鹅血清钙磷、碱性磷酸酶及胫骨钙磷的影响[J]. 中国家禽, 2009, 31(15): 16-20.
	WANG Z W, MOU X L, YANG G W, et al. Effects of dietary nutritional levels on calcium, phosphorus concentrations and ALK activity in serum and calcium, phosphorus contents in Tibia for geese[J]. China Poultry, 2009, 31(15): 16-20. (in Chinese with English abstract)
[16]	郝艳霜, 冯焯, 赵国先, 等. 日粮钙磷水平对育成期坝上长尾鸡生长性能和血液生化指标的影响[J]. 粮食与饲料工业, 2018(3): 47-50.
	HAO Y S, FENG Z, ZHAO G X, et al. Effects of dietary calcium and phosphorus levels on growth performance and blood biochemical indexes of the Bashang long-tailed chickens in rearing period[J]. Cereal & Feed Industry, 2018(3): 47-50. (in Chinese with English abstract)
[17]	陈冬梅. 植酸酶和柠檬酸对肉鸡生产性能及钙、磷利用率的影响[J]. 饲料工业, 2003, 24(1): 22-24.
	CHEN D M. Effects of phytase and citric acid on performance and utilization ratio of calcium and phosphorus in broilers[J]. Feed Industry, 2003, 24(1): 22-24. (in Chinese)
[18]	胡顺勇, 胡胜超, 郭均友, 等. 浅谈动物机体微量元素钙和磷的作用机理[J]. 山东畜牧兽医, 2020, 41(11): 76-77.
	HU S Y, HU S C, GUO J Y, et al. Discussion on the action mechanism of trace elements calcium and phosphorus in animal body[J]. Shandong Journal of Animal Science and Veterinary Medicine, 2020, 41(11): 76-77. (in Chinese)
[19]	STATHI A, MAMAIS M, CHRYSINA E D, et al. Anomeric spironucleosides of β-d-glucopyranosyl uracil as potential inhibitors of glycogen phosphorylase[J]. Molecules, 2019, 24(12): 2327.
[20]	AGIUS L. Role of glycogen phosphorylase in liver glycogen metabolism[J]. Molecular Aspects of Medicine, 2015, 46: 34-45.
[21]	ZOIS CHRISTOS E, HENDRIKS ANNE M, SYED H, et al. Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation[J]. Cell Death & Disease, 2022, 13(6): 573.

日粮 Dietary	比例 Proportion/ %	营养素 Nutrient	营养水平 Nutritional level
豆粕 Soybean meal, CP 43%	4.50	代谢能 Metabolic energy/ (MJ·kg^-1)	11.37
皮大麦 Leather barley	7.00	粗蛋白含量 Crude protein content/%	10.31
小麦 Wheat	10.00	粗脂肪含量 Crude fat content/%	3.28
碎米 Broken rice	50.00	粗纤维含量 Crude fiber content/%	7.51
米糠 Rice bran	15.00	钙含量 Calcium content/%	0.26
统糠 Series chaff 预混料Premix	12.00 1.50	有效磷含量 Available phosphorus content/%	0.24
合计Total	100.00

日粮 Dietary	比例 Proportion/ %	营养素 Nutrient	营养水平 Nutritional level
豆粕 Soybean meal, CP 43%	4.50	代谢能 Metabolic energy/ (MJ·kg^-1)	11.37
皮大麦 Leather barley	7.00	粗蛋白含量 Crude protein content/%	10.31
小麦 Wheat	10.00	粗脂肪含量 Crude fat content/%	3.28
碎米 Broken rice	50.00	粗纤维含量 Crude fiber content/%	7.51
米糠 Rice bran	15.00	钙含量 Calcium content/%	0.26
统糠 Series chaff 预混料Premix	12.00 1.50	有效磷含量 Available phosphorus content/%	0.24
合计Total	100.00

处理 Treatment	平均日增重 Average daily gain/g	耗料增重比 Feed/gain ratio
对照组Control group	44.64±16.17 a	6.09±2.56
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	27.09±6.43 b	6.33±1.37
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	34.09±15.72 ab	6.02±2.89

处理 Treatment	平均日增重 Average daily gain/g	耗料增重比 Feed/gain ratio
对照组Control group	44.64±16.17 a	6.09±2.56
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	27.09±6.43 b	6.33±1.37
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	34.09±15.72 ab	6.02±2.89

处理 Treatment	宰前活重 Weight	半净膛重 Semi-eviscerated weight	全净膛重 Eviscerated weight
对照组Control group	7.58±1.03 a	3.33±0.37 a	2.88±0.24 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.29±0.63 b	2.74±0.17 b	2.39±0.12 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	7.04±0.73 ab	3.12±0.23 ab	2.72±0.17 ab

Effect of dietary calcium and phosphorus level on Magang geese growth performance and liver gene expression

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 21

Related Articles 15

Recommended Articles

Metrics

Comments

处理 Treatment	粪磷含量 Content of calcium in feces	粪钙含量 Content of phosphorus in feces	血钙含量 Blood calcium concentration	血磷含量 Blood phosphorus concentration
对照组Control group	5.48±1.31	3.34±1.41 b	2.92±0.26	4.35±1.68
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	6.13±1.09	4.12±1.64 b	3.10±0.29	4.81±1.91
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	5.10±1.85	7.23±3.58 a	3.13±0.28	4.33±1.08

处理 Treatment	肝脏中GP活性 GP activity in liver/ (ng·mL^-1·g^-1)
对照组Control group	33.03±7.19 a
0.15%磷酸氢钙组 0.15% calcium hydrogen phosphate addition group	24.13±3.97 b
0.3%磷酸氢钙组 0.3% calcium hydrogen phosphate addition group	20.32±1.72 b

[1]	ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.
[2]	ZHANG Ruonan, MEN Xiaoming, QIN Kaipeng, WANG Binbin, WU Jie, DING Xiangbin, XU Ziwei, QI Keke. Comparative study on growth performance, carcass quality, meat performance and profitability of different crossbreed combinations of Lvjiahei pigs [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1203-1211.
[3]	TAO Peng, DING Xiaoya, YUE Zhichen, ZHAO Yanting, LEI Juanli, HU Qizan, ZANG Yunxiang, LI Biyuan. Genome-wide association study and candidate gene prediction of pectin content of pumpkin (Cucurbita moschata) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1244-1251.
[4]	HUANG Hao, TANG Tao, XU Zhenlan, ZHAO Xueping. Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale [J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 115-125.
[5]	ZHAN Mengqi, SU Aoxue, HOU Qian, ZHANG Haoyu, JIANG Xinrui, XU Yan. Uptake and accumulation of lindane in rice and its metabolomics [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2110-2121.
[6]	LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.
[7]	ZENG Hongxue, QU Xinghong. Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1558-1568.
[8]	HE Changxi, ZHENG Jianbo, MA Jianbo, JIA Yongyi, LIU Shili, JIANG Wenping, CHI Meili, CHENG Shun, LI Fei. Cloning and expression analysis of Runx2b in Culter alburnus [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1024-1031.
[9]	LI Yaping, JIN Fulai, HUANG Zonggui, ZHANG Tao, DUAN Xiaojing, JIANG Wu, TAO Zhengming, CHEN Jiadong. Identification and expression pattern analysis of glycoside hydrolase GH3 gene family in Dendrobium officinale [J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 790-799.
[10]	QIN Kaipeng, MEN Xiaoming, XU Ziwei. Research progress of umami substances and their formation mechanism in pork [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 719-728.
[11]	PENG Jiacheng, WU Yue, XU Jiehao, XIA Meiwen, QI Tianpeng, XU Haisheng. Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 247-253.
[12]	HU Lixia, ZHANG Jing, GAO Yanqiang, MAO Erye, HAN Kangning, YANG Yan, XIE Jianming. Effects of long-term magnesium stress on chlorophyll fluorescence characteristics and antioxidant properties of celery [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 295-307.
[13]	LIU Xiaolin, SUN Tingting, YANG Jie, HE Hengbin. Cloning and expression analysis of FLS gene of flavonol synthetase in Lilium auratum and L.speciosum var. gloriosoides [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 344-357.
[14]	YE Tao, SUN Qinyu, CHEN Weili, SHAN Wenshu, LIAN Wenxu, NIU Tingting, ZHANG Jiaxia. Research progress of bZIP gene family in plant-pathogenic fungi [J]. Acta Agriculturae Zhejiangensis, 2024, 36(12): 2885-2894.
[15]	DONG Biao, JI Rongchao, ZHANG Gansheng, WANG Jian. Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck [J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2456-2464.