Uptake and accumulation of lindane in rice and its metabolomics

doi:10.3969/j.issn.1004-1524.20230816

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 2110-2121.DOI: 10.3969/j.issn.1004-1524.20230816

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Uptake and accumulation of lindane in rice and its metabolomics

ZHAN Mengqi(), SU Aoxue, HOU Qian, ZHANG Haoyu, JIANG Xinrui, XU Yan()

School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China

Received:2023-06-29 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

The bioaccumulation, translocation and metabolomics of organochlorine pollutant lindane in the typical crop in China, rice (Oryza sativa L.) were investigated. The results revealed that under the stress of lindane contamination, the lindane concentration in rice roots, and above-ground parts (stems and leaves) had a positive correlation with the lindane concentration in the hydroponic solution, which indicated that rice had the ability to absorb lindane. Under different lindane concentrations in the hydroponic solution, the lindane accumulation in rice roots reached its peak on the second day and decreased sharply on the third day. In addition, lindane in rice roots could be transported radially to the above-ground parts, but the transport factor was 0.08-0.13. At subcellular level, the content of lindane in rice roots decreased as organelles>cytoderm>cytoplasm, which indicated that the organelle and cell wall were the main storage sites of lindane in rice roots. Under lindane stress, the changes of lipid metabolites were the most significant, with 43 up-regulated and 42 down-regulated. The content of isovitexin and other metabolites increased, while the content of asparagine and other metabolites decreased under lindane stres, and many metabolic pathways in plant were changed, among which, the sphingolipid metabolic pathway and phenylalanine metabolic pathway were greatly influenced.

Key words: lindane, rice, plant metabolism, uptake and transportation, agricultural product safety

CLC Number:

ZHAN Mengqi, SU Aoxue, HOU Qian, ZHANG Haoyu, JIANG Xinrui, XU Yan. Uptake and accumulation of lindane in rice and its metabolomics[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2110-2121.

Figures/Tables 6

Fig.1 Dynamic of lindane content in rice root under treatments

Fig.2 Changes of lindane content at rice subcellular level in root (A), lindane content in stem and leaf (B), and lipid content in root under treatments Bars marked without the same letters indicate significant difference at P<0.05.

Fig.3 Distribution of metabolites in rice root under lindane stress FC, Fold change, which is the ratio of the treatment grout and the control group.The same as below.

Fig.4 Sangi diagram of differential metabolites in rice root under lindane stress

Fig.5 The top ten metabolites with significant up-regulation (A) and down-regulation (B) of abundance in rice root under lindane stress

Fig.6 Changes of rice metabolic pathway under lindane stress Each circle represents a metabolic pathway, and the larger the circle, the stronger the correlation, and the redder the color, the bigger influence.

References 66

[1]	曹臻, 李军, 邴海健, 等. 我国北方地区森林土壤中有机氯农药的含量特征和空间分布研究[J]. 广东化工, 2022, 49(19): 137-141.
	CAO Z, LI J, BING H J, et al. Content characteristics and spatial distribution of organochlorine pesticides in forest soils in Northern China[J]. Guangdong Chemical Industry, 2022, 49(19): 137-141. (in Chinese with English abstract)
[2]	秦丽萍. 西双版纳蔬菜地土壤有机氯农药残留现状及农业绿色发展评价[J]. 热带农业科学, 2022, 42(10): 57-62.
	QIN L P. Organochlorine pesticide residues in vegetable soil and evaluation of agricultural green development in Xishuangbanna[J]. Chinese Journal of Tropical Agriculture, 2022, 42(10): 57-62. (in Chinese with English abstract)
[3]	郭子武. 笋用竹林地有机农药污染土壤微生物修复机理研究[D]. 北京: 中国林业科学研究院, 2008.
	GUO Z W. Study on bioremediation of organ-pesticides polluted soil of bamboo forest for shoot[D]. Beijing: Chinese Academy of Forestry, 2008. (in Chinese with English abstract)
[4]	翁昕. 负载型Pd催化剂对氯代有机污染物的液相催化加氢脱氯研究[D]. 南京: 南京大学, 2020.
	WENG X. Catalytic hydrodechlorination of chlorinated organic pollutants over supported Pd catalysts[D]. Nanjing: Nanjing University, 2020. (in Chinese with English abstract)
[5]	李君敬, 种雨彤, 唐李文, 等. 电催化还原脱氯法处理氯代有机废水研究进展[J]. 给水排水, 2021, 57(12): 158-167.
	LI J J, CHONG Y T, TANG L W, et al. Review of electrocatalytic hydrodechlorination method for treatment of chlorinated organic wastewater[J]. Water & Wastewater Engineering, 2021, 57(12): 158-167. (in Chinese with English abstract)
[6]	黄琎. 负载纳米零价铁同时去除废水中重金属与氯代有机污染物[D]. 绍兴: 绍兴文理学院, 2019.
	HUANG J. Simultaneous removeal of heavy metal and chlorinated organic pollutants in wastewater by supported nanoscale zero-valent iron[D]. Shaoxing: Shaoxing University, 2019. (in Chinese with English abstract)
[7]	李燕妮. Pd基催化剂对水中氯代有机污染物的加氢脱氯研究[D]. 上海: 东华大学, 2017.
	LI Y N. Study on hydrogenation and dechlorination of chlorinated organic pollutants in water by Pd-based catalysts[D]. Shanghai: Donghua University, 2017. (in Chinese with English abstract)
[8]	崔健, 都基众, 马宏伟, 等. 沈阳市城郊表层土壤有机污染评价[J]. 生态学报, 2012, 32(24): 7874-7882.
	CUI J, DU J Z, MA H W, et al. Assessment of organic pollution for surface soil in Shenyang suburbs[J]. Acta Ecologica Sinica, 2012, 32(24): 7874-7882. (in Chinese with English abstract)
[9]	YANG K X, KONG Y J, HUANG L Z, et al. Catalytic elimination of chlorinated organic pollutants by emerging single-atom catalysts[J]. Chemical Engineering Journal, 2022, 450: 138467.
[10]	王玉红. 紫花苜蓿(Medicago sativa)对有机氯农药DDT污染土壤的修复研究[D]. 南京: 南京林业大学, 2006.
	WANG Y H. Remediation of DDTs contaminated soil by Medicago sativa[D]. Nanjing: Nanjing Forestry University, 2006. (in Chinese with English abstract)
[11]	赵起越, 夏夜, 邹本东. 土壤中有机氯农药的前处理方法[J]. 分析试验室, 2022, 41(10): 1234-1240.
	ZHAO Q Y, XIA Y, ZOU B D. Pretreatment of organochlorine pesticides in soil[J]. Chinese Journal of Analysis Laboratory, 2022, 41(10): 1234-1240. (in Chinese with English abstract)
[12]	朱乐东. 生物酶作用下典型有机氯代污染物的降解和代谢活化机理研究[D]. 济南: 山东大学, 2021.
	ZHU L D. Study on degradation and metabolism activation mechanism of typical organochlorinated pollutants under the action of biological enzymes[D]. Jinan: Shandong University, 2021. (in Chinese with English abstract)
[13]	傅婉秋, 谢星光, 戴传超, 等. 植物-微生物联合对环境有机污染物降解的研究进展[J]. 微生物学通报, 2017, 44(4): 929-939.
	FU W Q, XIE X G, DAI C C, et al. Progress in the degradation of environmental organic pollutants by plant-microorganism combination[J]. Microbiology China, 2017, 44(4): 929-939. (in Chinese with English abstract)
[14]	刘贝贝, 陈歆, 陈淼, 等. 根系分泌物在土壤有机污染物降解中的作用[J]. 热带农业科学, 2013, 33(10): 47-52.
	LIU B B, CHEN X, CHEN M, et al. Role of root exudates in the degradation of organic pollutants in soils[J]. Chinese Journal of Tropical Agriculture, 2013, 33(10): 47-52. (in Chinese with English abstract)
[15]	张福金. 老化HCH-DDT污染土壤的作物自修复及其机理研究[D]. 呼和浩特: 内蒙古大学, 2013.
	ZHANG F J. Study on the process of crops self-remediation and its phytoremediation mechanism in soils contaminated by ageing HCH/DDT[D]. Hohhot: Inner Mongolia University, 2013. (in Chinese with English abstract)
[16]	MATTINA M J, IANNUCCI-BERGER W, DYKAS L. Chlordane uptake and its translocation in food crops[J]. Journal of Agricultural and Food Chemistry, 2000, 48(5): 1909-1915. DOI PMID
[17]	HUELSTER A, MUELLER J F, MARSCHNER H. Soil-plant transfer of polychlorinated dibenzo-p-dioxins and dibenzofurans to vegetables of the cucumber family (Cucurbitaceae)[J]. Environmental Science & Technology, 1994, 28(6): 1110-1115.
[18]	WHITE J C, PARRISH Z D, ISLEYEN M, et al. Uptake of weathered p, p’-DDE by plant species effective at accumulating soil elements[J]. Microchemical Journal, 2005, 81(1): 148-155.
[19]	MIGLIORANZA K S B, AIZPÚN DE MORENO J E, MORENO V J, et al. Fate of organochlorine pesticides in soils and terrestrial biota of “Los Padres” pond watershed, Argentina[J]. Environmental Pollution, 1999, 105(1): 91-99.
[20]	BURKEN J G. Uptake and metabolism of organic compounds: green-liver model[M]//MCCUTCHEON S C, SCHNOOR J L. Phytoremediation: transformation and control of contaminants. New York: John Wiley & Sons, Inc. 2003: 59-84.
[21]	BEATH J M. Consider phyto remediation for waste site cleanup[J]. Chemical Engineering Progress, 2000, 96(7):61-69.
[22]	HAHAM H, OREN A, CHEFETZ B. Insight into the role of dissolved organic matter in sorption of sulfapyridine by semiarid soils[J]. Environmental Science & Technology, 2012, 46(21): 11870-11877.
[23]	王志刚, 徐晓燕. 有机污染土壤植物修复机理的研究现状[J]. 环境科学与技术, 2006, 29(2): 106-108.
	WANG Z G, XU X Y. Mechanism of phytoremediation for organic contaminated soil[J]. Environmental Science & Technology, 2006, 29(2): 106-108. (in Chinese with English abstract)
[24]	刘细祥. 六氯苯在植物中分布及吸收积累机理研究[D]. 武汉: 华中科技大学, 2008.
	LIU X X. Studies on distribution, uptake and accumulation progress of hexachlorobenzene in plants[D]. Wuhan: Huazhong University of Science and Technology, 2008. (in Chinese with English abstract)
[25]	万大娟, 贾晓珊, 陈娴. 多氯代有机污染物胁迫下植物某些根系分泌物的变化[J]. 中山大学学报(自然科学版), 2007, 46(1): 110-113.
	WAN D J, JIA X S, CHEN X. Effects of PCOPs on some root exudates of plants[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2007, 46(1): 110-113. (in Chinese with English abstract)
[26]	李昊聪. 戊唑醇胁迫下水稻植株的代谢响应及其对稻米品质影响[D]. 镇江: 江苏大学, 2022.
	LI H C. Metabolic response of rice plants under tebuconazole stress and its effect on rice quality[D]. Zhenjiang: Jiangsu University, 2022. (in Chinese with English abstract)
[27]	PHILLIPS L A, GERMIDA J J, FARRELL R E, et al. Hydrocarbon degradation potential and activity of endophytic bacteria associated with prairie plants[J]. Soil Biology and Biochemistry, 2008, 40(12): 3054-3064.
[28]	ZHAO L J, XIE J F, ZHANG H, et al. Changes in metabolites in maize seedlings under chlorsulfuron and cadmium stress[J]. The Journal of Agricultural Science, 2016, 154(5): 890-913.
[29]	NAVARRO-REIG M, JAUMOT J, PIÑA B, et al. Metabolomic analysis of the effects of cadmium and copper treatment in Oryza sativa L. using untargeted liquid chromatography coupled to high resolution mass spectrometry and all-ion fragmentation[J]. Metallomics: Integrated Biometal Science, 2017, 9(6): 660-675.
[30]	ZHANG H L, LU L, ZHAO X P, et al. Metabolomics reveals the “invisible” responses of spinach plants exposed to CeO₂ nanoparticles[J]. Environmental Science & Technology, 2019, 53(10): 6007-6017.
[31]	谭冬飞. 全氟烷基酸农用水中分布及对大豆叶片代谢效应[D]. 沈阳: 沈阳农业大学, 2018.
	TAN D F. The distribution of perfluorocompound water in agriculture and its effect on metabolism of soybean leaves[D]. Shenyang: Shenyang Agricultural University, 2018. (in Chinese with English abstract)
[32]	KUMAR R, BOHRA A, PANDEY A K, et al. Metabolomics for plant improvement: status and prospects[J]. Frontiers in Plant Science, 2017, 8: 1302. DOI PMID
[33]	赵明丽. 低氮胁迫下野大豆(Glycine soja Sieb. et Zucc.)幼苗叶片生理特性及代谢组学研究[D]. 长春: 东北师范大学, 2020.
	ZHAO M L. Physiological characteristics and metabolomics reveal the tolerance mechanism to low nitrogen in Glycine soja Sieb. et Zucc. seedling leaves[D]. Changchun: Northeast Normal University, 2020. (in Chinese with English abstract)
[34]	李小青. 化学农药对青菜及其根际微环境的影响机制研究[D]. 桂林: 桂林理工大学, 2021.
	LI X Q. Effect of chemical pesticides on plant tissues and rhizospheric environment[D]. Guilin: Guilin University of Technology, 2021. (in Chinese with English abstract)
[35]	华欣. 林丹对秀丽隐杆线虫的毒性效应及作用机制研究[D]. 兰州: 兰州交通大学, 2020.
	HUA X. Toxicological effects and mechanism of pesticide lindane on nematode caenorhabditis elegans[D]. Lanzhou: Lanzhou Jiatong University, 2020. (in Chinese with English abstract)
[36]	HUANG S D, TAN L, ZHU H, et al. Root damages induced by extended phytotoxic effects of cadmium pre-exposure against subsequent lindane uptake in rice seedlings[J]. Environmental and Experimental Botany, 2021, 189: 104553.
[37]	HUANG S D, SHENG G D. Lindane uptake and translocation by rice seedlings (Oryza sativa L.) under different culture patterns and triggered biomass re-allocation[J]. Chemosphere, 2021, 262: 127831.
[38]	张生盛. 氮源对斜生栅藻生长及脂质积累影响[D]. 南宁: 广西大学, 2021.
	ZHANG S S. Effects of nitrogen sources on growth and lipid accumulation of Scenedesmus obliquus[D]. Nanning: Guangxi University, 2021. (in Chinese with English abstract)
[39]	孙彩丽. 卷枝毛霉利用外源油积累脂质的机制研究[D]. 淄博: 山东理工大学, 2021.
	SUN C L. Study on the mechanism of lipid accumulation by exogenous oil in Mucor circinelloides[D]. Zibo: Shandong University of Technology, 2021. (in Chinese with English abstract)
[40]	VASILEV N, BOCCARD J, LANG G, et al. Structured plant metabolomics for the simultaneous exploration of multiple factors[J]. Scientific Reports, 2016, 6: 37390. DOI PMID
[41]	ZELENA E, DUNN W B, BROADHURST D, et al. Development of a robust and repeatable UPLC-MS method for the long-term metabolomic study of human serum[J]. Analytical Chemistry, 2009, 81(4): 1357-1364. DOI PMID
[42]	WANT E J, MASSON P, MICHOPOULOS F, et al. Global metabolic profiling of animal and human tissues via UPLC-MS[J]. Nature Protocols, 2013, 8(1): 17-32. DOI PMID
[43]	张俪倢. 水稻对典型有机磷酸酯的吸收、迁移及转化研究[D]. 大连: 大连理工大学, 2021.
	ZHANG L J. Uptake, translocation, and biotransformation of organophosphate esters in rice (Oryza Sativa L.)[D]. Dalian: Dalian University of Technology, 2021. (in Chinese with English abstract)
[44]	陈冬升. 多环芳烃在植物体内的亚细胞分配[D]. 南京: 南京农业大学, 2009.
	CHEN D S. Subcellular distribution of polycyclic aromatic hydrocarbons in plants[D]. Nanjing: Nanjing Agricultural University, 2009. (in Chinese with English abstract)
[45]	陈冬升, 凌婉婷, 张翼, 等. 几种植物根亚细胞中菲的分配[J]. 环境科学, 2010, 31(5): 1339-1344.
	CHEN D S, LING W T, ZHANG Y, et al. Subcellular distribution of phenanthrene in plant root tissues[J]. Environmental Science, 2010, 31(5): 1339-1344. (in Chinese with English abstract)
[46]	胡蓓蓓. 有机磷酸酯(OPEs)在土壤-植物系统中的吸收、转运和迁移行为研究[D]. 广州: 中国科学院广州地球化学研究所, 2021.
	HU B B. Studies on the uptake, transformation and migration of organophosphorus esters (OPEs) in the soil-plant systems[D]. Guangzhou: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, 2021. (in Chinese with English abstract)
[47]	鞠超. 小麦植株对七种农药的吸收、转运及其机制[D]. 杭州: 浙江大学, 2020.
	JU C. Uptake, translocation of seven pesticides by wheat plant and their mechanisms[D]. Hangzhou: Zhejiang University, 2020. (in Chinese with English abstract)
[48]	沈小明, 王梅农, 代静玉. 不同浓度条件下玉米吸收菲的水培实验研究[J]. 农业环境科学学报, 2006, 25(5): 1148-1152.
	SHEN X M, WANG M N, DAI J Y. Uptake of phenanthrene by maize from hydroponic solutions with different concentrations[J]. Journal of Agro-Environment Science, 2006, 25(5): 1148-1152. (in Chinese with English abstract)
[49]	COLLINS C, FRYER M, GROSSO A. Plant uptake of non ionic organic chemicals[J]. Environmental Science & Technology, 2006, 40(1): 45-52.
[50]	赵刘清. 三唑类手性杀菌剂氟环唑和戊唑醇对蔬菜代谢组和脂质组的立体选择性影响[D]. 北京: 中国农业科学院, 2021.
	ZHAO L Q. Stereoselective effect of chiral triazole fungicides epoxiconazole and tebuconazole on the metabolism and lipid metabolism of vegetables[D]. Beijing: Chinese Academy of Agricultural Sciences, 2021. (in Chinese with English abstract)
[51]	鲁士民. 黄酮类化合物在植物生理中的效应[J]. 植物生理学通讯, 1981, 17(2): 17-20.
	LU S M. Effects of flavonoids on plant physiology[J]. Plant Physiology Communications, 1981, 17(2): 17-20. (in Chinese)
[52]	庄林武. 芹菜叶黄酮类化合物分离纯化及其结构鉴定[D]. 扬州: 扬州大学, 2016.
	ZHUANG L W. Isolation, purification and structural identification of flavonoids from celery leaves[D]. Yangzhou: Yangzhou University, 2016. (in Chinese with English abstract)
[53]	LIU Z H, LIU Y X, PU Z E, et al. Regulation, evolution, and functionality of flavonoids in cereal crops[J]. Biotechnology Letters, 2013, 35(11): 1765-1780. DOI PMID
[54]	刘润华, 江文波, 余迪求. 植物鞘脂的结构、代谢途径及其功能[J]. 植物学报, 2009, 44(5): 619-628.
	LIU R H, JIANG W B, YU D Q. Structure, metabolic pathway and function of sphingolipids in plants[J]. Chinese Bulletin of Botany, 2009, 44(5): 619-628. (in Chinese with English abstract)
[55]	钟宛儒, 金君, 辛树权. 多胺提升植物抗逆境能力研究[J]. 长春师范大学学报, 2022, 41(10): 113-118.
	ZHONG W R, JIN J, XIN S Q. Research on polyamines improving plant resilience to stress[J]. Journal of Changchun Normal University, 2022, 41(10): 113-118. (in Chinese with English abstract)
[56]	汤章城. 逆境条件下植物脯氨酸的累积及其可能的意义[J]. 植物生理学通讯, 1984, 20(1): 15-21.
	TANG Z C. Accumulation of proline in plants under adversity and its possible significance[J]. Plant Physiology Communications, 1984, 20(1): 15-21. (in Chinese)
[57]	秦如意. 天冬酰胺合成酶OsASN1对水稻生长发育和代谢的影响[D]. 南京: 南京农业大学, 2020.
	QIN R Y. Effects of asparagine synthase OsASN1 on development and metabolism of rice[D]. Nanjing: Nanjing Agricultural University, 2020. (in Chinese with English abstract)
[58]	KOZHEVNIKOVA A D, SEREGIN I V, ERLIKH N T, et al. Histidine-mediated xylem loading of zinc is a species-wide character in Noccaea caerulescens[J]. The New Phytologist, 2014, 203(2): 508-519.
[59]	王翔, 汤承浩, 谭荣, 等. 酰胺类化合物的合成及农药生物活性研究进展[J]. 精细化工中间体, 2016, 46(6): 7-14.
	WANG X, TANG C H, TAN R, et al. Recent advances in the study on synthesis and pesticide activity of amide derivatives[J]. Fine Chemical Intermediates, 2016, 46(6): 7-14. (in Chinese with English abstract)
[60]	王浩, 张松杰, 李航, 等. 植物壬二酸的研究进展[J]. 植物生理学报, 2022, 58(3): 483-491.
	WANG H, ZHANG S J, LI H, et al. Research progress of azelaic acid in plants[J]. Plant Physiology Journal, 2022, 58(3): 483-491. (in Chinese with English abstract)
[61]	HOWE G A, JANDER G. Plant immunity to insect herbivores[J]. Annual Review of Plant Biology, 2008, 59: 41-66. PMID
[62]	秦晶晶, 孙春玉, 张美萍, 等. 植物UDP-糖基转移酶分类、功能以及进化[J]. 基因组学与应用生物学, 2018, 37(1): 440-450.
	QIN J J, SUN C Y, ZHANG M P, et al. Classification, function and evolution of plant UDP-glycosyltransferase[J]. Genomics and Applied Biology, 2018, 37(1): 440-450. (in Chinese with English abstract)
[63]	何方成. 含磺胺取代嘌呤核苷类衍生物的设计合成和抗植物病毒活性及其作用机制研究[D]. 贵阳: 贵州大学, 2019.
	HE F C. Design and synthesis of sulphonamine-containing substituted purine nucleosides derivatives and study on their anti-plant virus activity and mechanism of action[D]. Guiyang: Guizhou University, 2019. (in Chinese with English abstract)
[64]	MAHDAVI V, FARIMANI M M, FATHI F, et al. A targeted metabolomics approach toward understanding metabolic variations in rice under pesticide stress[J]. Analytical Biochemistry, 2015, 478: 65-72. DOI PMID
[65]	李贺欢. 鞘脂代谢调控油菜生长和抗非生物逆境研究[D]. 武汉: 华中农业大学, 2018.
	LI H H. Regulation of growth and abiotic stress tolerance by sphingolipid metabolism in Brassica napus L.[D]. Wuhan: Huazhong Agricultural University, 2018. (in Chinese with English abstract)
[66]	GUO Q Q, HE Z Y, LIU X W, et al. High-throughput non-targeted metabolomics study of the effects of perfluorooctane sulfonate (PFOS) on the metabolic characteristics of A. thaliana leaves[J]. Science of the Total Environment, 2020, 710: 135542.

Uptake and accumulation of lindane in rice and its metabolomics

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 66

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Haofeng, LIN Zhaoyang, SHEN Zhicheng. A transgenic rice resistant to glyphosate and flazasulfuron [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 1957-1968.
[2]	CHEN Yutiao, YAN Chuan, HONG Xiaofu, SONG Jiayu. Effects of submergence at tillering stage on growth characters, yield formation and potassium uptake of japonica inbred rice [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 1990-1999.
[3]	SHAO Yaxu, LIU Tao, WANG Shicheng, YAN Lei. Screening of proportions and molding conditions of seeding substrate with straw and organic fertilizer [J]. Acta Agriculturae Zhejiangensis, 2024, 36(8): 1856-1866.
[4]	PAN Zhijun, WU Xiaowen, WU Chenyang, CHENG Yu, CHEN Long, ZHANG Xiaohong, ZHANG Jinshan, ZHOU Bing, JIANG Bo, ZHANG Wenjing, CHE Zhao, SONG He. Analysis of yield and utilization of temperature and light resources of different types of ratoon rice varieties in central Anhui, China [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1492-1501.
[5]	TANG Jinyu, HUANG Fuyong, DAI Yangxin, LOU Bao, GUO Shuirong. The temporal characteristic of plankton community and their relationship with shrimp growth in the co-cultural farming of rice and redclaw crayfish (Cherax quadricarinatus) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1537-1547.
[6]	XIONG Rui, OUYANG Ning, OU Xi, ZHONG Kangyu, ZHOU Wentao, WANG Hongrui, LONG Pan, XU Ying, FU Zhiqiang. Effect of straw returning and tillage method on soil aggregates and carbon, nitrogen content in double-season rice [J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1347-1356.
[7]	ZHAO Liming, WANG Yaxin, JIANG Wenxin, DUAN Shaobiao, SHEN Xuefeng, ZHENG Dianfeng, FENG Naijie. Effects of plant growth regulators on yield, quality and photosynthetic characteristics of high-quality japonica rice [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1003-1014.
[8]	WANG Zhuoquan, LIN Zhenpeng, CHEN Xudong, QIAN Bin, ZHAI Rongrong, YE Shenghai, YE Jing, WU Mingming, ZHU Guofu, ZHANG Xiaoming. Effects of glutinous rice characteristics of different glutinous rice varieties on the quality of Shaoxing rice wine [J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 773-779.
[9]	DONG Aiqin, CHEN Yuanhua, YANG Tao, XU Changxu, CHENG Liqun, XIE Jie. Effect of application of lime with Chinese milk vetch on the cadmium uptake in rice [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 600-612.
[10]	YE Qin, MENG Xianghe, CHEN Lihong. Effects of rice bran curing on physicochemical quality and fat oxidation characteristics of sauce duck [J]. Acta Agriculturae Zhejiangensis, 2024, 36(3): 634-642.
[11]	ZHANG Bin, YUAN Zhihui, PENG Lujun, ZHOU Xiangping, ZHOU Deying, WANG Xichun. Fermented rice husk affects the growth and development of tobacco seedlings by enhancing nitrogen metabolism pathway [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 237-246.
[12]	ZHENG Han, DING Wenjin, HE Zhaoliang, HOU Fan, DAI Binfeng, ZHONG Liequan, ZHANG Haipeng, YANG Yong. Research progress on effects of high temperature on growth and development of rice during panicle initiation stage and mitigation measures [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 470-480.
[13]	YANG Xifan, GUO Bin, QIU Gaoyang, LIU Junli, TONG Wenbin, YANG Haijun, ZHU Weidong, MAO Congyan. Inhibiting effects of immobilization agents on cadmium, lead and arsenic in rice production [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 1-8.
[14]	LUO Yingjie, CUI Weijun, WANG Zhonghua, WU Yueyan, LIN Hongyou, ZHOU Jie, YAN Chengqi, WANG Xuming. Interaction analysis between rice ubiquitin ligase D3 and the disease resistance associated protein VOZ2 [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 9-17.
[15]	ZHANG Siyu, LIN Chaoyang, YE Yuxuan, SHEN Zhicheng. Characterization of transgenic insect resistance and glyphosate tolerance rice expressing cry1Ab-vip3Af2 and cp4-epsps [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1823-1833.