Effects of enzyme-assisted extraction on composition and gut microbiota regulation function of Dendrobium officinale polysaccharide

doi:10.3969/j.issn.1004-1524.20231114

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 2099-2109.DOI: 10.3969/j.issn.1004-1524.20231114

• Food Science • Previous Articles Next Articles

Effects of enzyme-assisted extraction on composition and gut microbiota regulation function of Dendrobium officinale polysaccharide

ZHANG Ni¹(), TAO Wenyang², LUO Mengfan², ZHOU Wanyi², ZHENG Xiaojie³, LI Yanpo³, JIN Huoxi¹^,^*(), YANG Ying²^,^*()

1. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316002, Zhejiang, China
2. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. Wenzhou Vocational College of Science and Technology (Wenzhou Academy of Agricultural Sciences), Wenzhou 325000, Zhejiang, China

Received:2023-09-21 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

Enzymatic hydrolysis may alter the number or structure of certain groups within the polysaccharide molecule, thus altering its properties. To determine the effect of enzymatic hydrolysis on the structure and function of Dendrobium officinale polysaccharide, this study selected the same species of D. officinale fresh stems as raw materials, and obtained different structures of the same matrix polysaccharide through neutral protease, maltose amylase, and cellulase-assisted extraction. Then, analyzing structural differences between them, and studying their various regulatory functions to intestinal flora using an in vitro simulated fermentation system. The results showed that compared with unenzymatically hydrolyzed samples, neutral protease-assisted extraction increased the degree of polymerization of polysaccharides, while the intervention of cellulase and maltose amylase significantly reduced the degree of polysaccharide polymerization and changed their monosaccharide composition. The production of CH₄ and H₂S in the neutral protease-treated group increased significantly, while the production of butyric acid and valeric acid in the cellulase-treated group increased significantly. Polysaccharides improved the composition of intestinal flora, especially the cellulase-treated group. The types and relative abundance of intestinal cornerstone bacteria as well as beneficial bacteria, such as Bifidobacterium, Lactobacillus, Faecalibacterium and Roseburia, increased significantly, while the relative abundance of opportunistic pathogenic bacteria, such as Dialister and Dorea, decreased significantly. There were nine bacterial genera that were significantly positively correlated with butyric acid and valeric acid levels, and eight of them were enriched in the cellulase-treated group. Enzymatic hydrolysis-assisted extraction changes the structure of D. officinale polysaccharides, thereby changing its regulatory functions to intestinal flora. This study provides a reference for the targeted production of polysaccharides with different functions.

Key words: Dendrobium officinale, polysaccharides, enzymatic hydrolysis, intestinal flora, in vitro fermentation

CLC Number:

R285

ZHANG Ni, TAO Wenyang, LUO Mengfan, ZHOU Wanyi, ZHENG Xiaojie, LI Yanpo, JIN Huoxi, YANG Ying. Effects of enzyme-assisted extraction on composition and gut microbiota regulation function of Dendrobium officinale polysaccharide[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2099-2109.

Figures/Tables 8

Table 1 Effect of extraction method on peak molecular weight of Dendrobium officinale polysaccharides u

提取方式 Extraction mode	峰值分子量Peak molecular weight
提取方式 Extraction mode	组分1 Component 1	组分2 Component 2
未酶解No enzymolysis	568 547	2 028
中性蛋白酶Neutral protease	892 441	2 228
麦芽糖淀粉酶Maltose amylase	248 758	1 745
纤维素酶Cellulase	22 044	1 356

Table 2 Effect of extraction method on monosaccharide composition from Dendrobium officinale

提取方式 Extraction mode	摩尔比Molar ratio
提取方式 Extraction mode	阿拉伯糖Arabinose	半乳糖Galactose	葡萄糖Glucose	甘露糖Mannose
未酶解No enzymolysis	0.003	0.005	1	1.83
中性蛋白酶Neutral protease	0.001	0.002	1	1.65
麦芽糖淀粉酶Maltose amylase	0	0	1	1.67
纤维素酶Cellulase	0.001	0.002	1	0.84

Fig.1 Effect of Dendrobium officinale polysaccharides on intestinal microbial fermentation gas Bc, Blank control; Nc, No enzymolysis control; Mal, Maltose amylase group; Cel, Cellulase group; Neu, Neutral protease group. A scatterplot without the same lowercase letter indicates significant differences between treatments (P<0.05). The same as below.

Fig.2 Effect of Dendrobium officinale polysaccharides on the content of short-chain fatty acids

Fig.3 Effect of polysaccharides from Dendrobium officinale on α diversity of intestinal flora

Fig.4 Effect of polysaccharides from Dendrobium officinale on β diversity of intestinal flora A, Principal co-ordinates analysis; B, Non-metric multi-dimensional scaling.

Fig.5 Effect of polysaccharides from Dendrobium officinale on intestinal flora community composition A, Composition difference at phylum level; B, Multi-group comparison difference at genus level.

Fig.6 Effect of polysaccharides from Dendrobium officinale on on species composition difference and correlation of intestinal flora A, Histogram of distribution of significantly different LDA values among blank control, no enzymolysis control and maltose amylase groups; B, Histogram of distribution of significantly different LDA values among blank control, no enzymolysis control and cellulase groups; C, Histogram of distribution of significantly different LDA values among blank control, no enzymolysis control and neutral protease groups; D, Correlation analysis diagram of intestinal flora at genus level, * represented 0.01<P≤0.05, ** represented 0.001<P≤0.01, *** represented P≤0.001.

References 27

[1]	TAO S C, LEI Z X, HUANG K W, et al. Structural characterization and immunomodulatory activity of two novel polysaccharides derived from the stem of Dendrobium officinale Kimura et Migo[J]. Journal of Functional Foods, 2019, 57: 121-134.
[2]	YUAN Y D, ZUO J J, ZHANG H Y, et al. Transcriptome and metabolome profiling unveil the accumulation of flavonoids in Dendrobium officinale[J]. Genomics, 2022, 114(3): 110324.
[3]	LIU W, YAN R, ZHANG L. Dendrobium sonia polysaccharide regulates immunity and restores the dysbiosis of the gut microbiota of the cyclophosphamide-induced immunosuppressed mice[J]. Chinese Journal of Natural Medicines, 2019, 17(8): 600-607.
[4]	LIANG J, CHEN S X, HU Y D, et al. Protective roles and mechanisms of Dendrobium officinal polysaccharides on secondary liver injury in acute colitis[J]. International Journal of Biological Macromolecules, 2018, 107: 2201-2210.
[5]	CHEN W H, WU J J, LI X F, et al. Isolation, structural properties, bioactivities of polysaccharides from Dendrobium officinale Kimura et. Migo: a review[J]. International Journal of Biological Macromolecules, 2021, 184: 1000-1013.
[6]	YANG K, LU T T, ZHAN L H, et al. Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell[J]. International Journal of Biological Macromolecules, 2020, 149: 320-330.
[7]	DUAN H T, ER-BU A G, DONGZHI Z M, et al. Alkaloids from Dendrobium and their biosynthetic pathway, biological activity and total synthesis[J]. Phytomedicine, 2022, 102: 154132.
[8]	唐军, 杨欣, 杨鑫, 等. 附子多糖的结构、构效关系与生物活性研究进展[J]. 中国中药杂志, 2023, 48(20): 5410-5418.
	TANG J, YANG X, YANG X, et al. Research progress on structure, structure-activity relationship, and biological activity of Aconiti Lateralis Radix Praeparata polysaccharides[J]. China Journal of Chinese Materia Medica, 2023, 48(20): 5410-5418. (in Chinese with English abstract)
[9]	黄俊彬, 黄丹丹, 陈欢欢, 等. 铁皮石斛多糖分子量测定及其影响因素分析[J]. 食品工业科技, 2017, 38(7): 81-85.
	HUANG J B, HUANG D D, CHEN H H, et al. Determination of molecular weight of Dendrobium officinale polysaccharides and its influencing factors[J]. Science and Technology of Food Industry, 2017, 38(7): 81-85. (in Chinese with English abstract)
[10]	罗秋莲, 唐专辉, 张雪凤, 等. 铁皮石斛多糖的分离纯化及其结构研究[J]. 广西大学学报(自然科学版), 2016, 41(6): 2060-2066.
	LUO Q L, TANG Z H, ZHANG X F, et al. Isolation, purification, and chemical composition analysis of polysaccharides from Dendrobium officinale[J]. Journal of Guangxi University(Natural Science Edition), 2016, 41(6): 2060-2066. (in Chinese with English abstract)
[11]	路婧, 夏雪娟, 黄骏楠, 等. 不同富营养培养基对人肠道菌群的体外培养效果比较分析[J/OL]. 食品与发酵业, [2024-07-22]. https://doi.org/10.13995/j.cnki.11-1802/ts.038595.
	LU J, XIA X J, HUANG J N, et al. Comparative analysis of in vitro culture effect of different eutrophic medium on human intestinal flora[J/OL]. Food and fermentation industry, [2024-07-22]. https://doi.org/10.13995/j.cnki.11-1802/ts.038595. (in Chinese with English abstract)
[12]	邹雄亮, 刘悦, 朱沅如, 等. 顶空气相法测定小鼠粪便短链脂肪酸[J]. 广州化工, 2021, 49(9): 106-110.
	ZOU X L, LIU Y, ZHU Y R, et al. Determination of short chain fatty acids in mice feces by headspace gas chromatography[J]. Guangzhou Chemical Industry, 2021, 49(9): 106-110. (in Chinese with English abstract)
[13]	LAI C H, HUO C Y, XU J, et al. Critical review on the research of chemical structure, bioactivities, and mechanism of actions of Dendrobium officinale polysaccharide[J]. International Journal of Biological Macromolecules, 2024, 263: 130315.
[14]	XIA C M, ZHANG R F, JIA X C, et al. In vitro human gut microbiota fermentation of litchi pulp polysaccharides as affected by Lactobacillus pre-treatment[J]. Food Chemistry, 2024, 445: 138734.
[15]	XU B, SONG S Q, YAO L Y, et al. Digestion under saliva, simulated gastric and small intestinal conditions and fermentation in vitro by human gut microbiota of polysaccharides from Ficus carica Linn[J]. Food Hydrocolloids, 2024, 146: 109204.
[16]	RASOULI-SARAVANI A, JAHANKHANI K, MORADI S, et al. Role of microbiota short-chain fatty acids in the pathogenesis of autoimmune diseases[J]. Biomedicine & Pharmacotherapy, 2023, 162: 114620.
[17]	PANT K, VENUGOPAL S K, LORENZO PISARELLO M J, et al. The role of gut microbiome-derived short-chain fatty acid butyrate in hepatobiliary diseases[J]. The American Journal of Pathology, 2023, 193(10): 1455-1467.
[18]	LAU H C H, ZHANG X, JI F F, et al. Lactobacillus acidophilus suppresses non-alcoholic fatty liver disease-associated hepatocellular carcinoma through producing valeric acid[J]. EBioMedicine, 2024, 100: 104952.
[19]	付域泽, 焦帅, 张乃锋. 产丁酸菌的产酸机制及其在调控肠道健康中的作用研究进展[J]. 畜牧兽医学报, 2022, 53(12): 4148-4158. DOI
	FU Y Z, JIAO S, ZHANG N F. Research progress of acidogenic mechanism of butyrate-producing bacteria and its regulation on intestinal health[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4148-4158. (in Chinese with English abstract) DOI
[20]	秦振宁, 肖睿铭, 梁书利, 等. 鳙鱼肠道菌群多样性及潜在益生菌的分离鉴定[J]. 现代食品科技, 2023, 39(5): 41-52.
	QIN Z N, XIAO R M, LIANG S L, et al. Diversity of gut microbiota in bighead carp and the isolation and identification of potential probiotics[J]. Modern Food Science and Technology, 2023, 39(5): 41-52. (in Chinese with English abstract)
[21]	徐磊, 罗小泉, 瞿萍, 等. 大鼠肥胖抑郁模型建立及肠道菌群多样性的性别差异研究[J]. 中国实验动物学报, 2023, 31(6): 743-755.
	XU L, LUO X Q, QU P, et al. Establishment of an obesity depression model in rats and study of sex differences in gut flora diversity[J]. Acta Laboratorium Animalis Scientia Sinica, 2023, 31(6): 743-755. (in Chinese with English abstract)
[22]	CAI M, ZHU H, XU L, et al. Structure, anti-fatigue activity and regulation on gut microflora in vivo of ethanol-fractional polysaccharides from Dendrobium officinale[J]. International Journal of Biological Macromolecules, 2023, 234: 123572.
[23]	ARBABI F, SHAPOURY R, HAGHI F, et al. Investigating the bacterial profiles of Lactobacillus, Bifidobacterium, Actinobacteria, Fusobacterium, Firmicutes, and Bacteroides in stool samples from patients with severe depression and healthy individuals[J]. Psychoneuroendocrinology, 2024: 107090.
[24]	MADIYAL M, BHAT U P, SACHIN C R. A rare case report of meningoencephalitis caused by Streptococcus porcinus[J]. Indian Journal of Medical Microbiology, 2024, 50: 100660.
[25]	DONG J J, WANG W J, ZHENG G D, et al. In vitro digestion and fermentation behaviors of polysaccharides from Choerospondias axillaris fruit and its effect on human gut microbiota[J]. Current Research in Food Science, 2024, 8: 100760.
[26]	卓萍, 刘威, 刘君琴, 等. 一株人消化道中韦荣氏球菌的分离、鉴定及其代谢特性研究[J]. 微生物学通报, 2016, 43(6): 1253-1261.
	ZHUO P, LIU W, LIU J Q, et al. Isolation, identification and metabolism of a Veillonella strain in human gut[J]. Microbiology China, 2016, 43(6): 1253-1261. (in Chinese with English abstract)
[27]	YAO Y, YAN L J, CHEN H, et al. Cyclocarya paliurus polysaccharides alleviate type 2 diabetic symptoms by modulating gut microbiota and short-chain fatty acids[J]. Phytomedicine, 2020, 77: 153268.

Effects of enzyme-assisted extraction on composition and gut microbiota regulation function of Dendrobium officinale polysaccharide

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