Effects of Hyriopsis cumingii polysaccharides on growth performance, antioxidant activity and immune function in growing broiler

doi:10.3969/j.issn.1004-1524.2023.03.07

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 547-555.DOI: 10.3969/j.issn.1004-1524.2023.03.07

• Animal Science • Previous Articles Next Articles

Effects of Hyriopsis cumingii polysaccharides on growth performance, antioxidant activity and immune function in growing broiler

XIA Lunbin¹^,²(), MA Longlong¹, QIAO Deliang¹^,², HE Yanfei¹^,², JIANG Ping¹^,²

1. College of Biological and Pharmaceutical Engineering, West Anhui University, Lu’an 237012, Anhui, China
2. Engineering Laboratory of Anhui Province for Conservation and Sustainable Utilization of Chinese Medicine Resources, Lu’an 237012, Anhui, China

Received:2021-12-14 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

The objective of this study was to investigate the effects of Hyriopsis cumingii polysaccharides (HCP) on growth performance, antioxidant activity and immune function in growing broiler. A total of 240 one-day-old Arbor Acres (AA) broiler chickens were randomly allotted into 4 groups, with 6 replicates in each group and 10 chickens in each replicate. Normal control group (HCP-0) was fed with basal diet, while low dose group (HCP-1), medium dose group (HCP-2) and high dose group (HCP-3) were supplemented with 200, 400 and 800 mg·kg^-1 HCP in basal diet, respectively. The experimental period was 42 days. The results showed as follows:1) Compared with HCP-0 group, the ADG of broilers in HCP-2 group and HCP-3 group was extremely significantly increased (P<0.01), and there was no significant difference in ADFI and F/G among all groups(P>0.05). 2) Compared with the HCP-0 group, the activities of T-AOC, T-SOD and GSH-Px in serum of broilers in HCP-2 group and HCP-3 group were significantly increased(P<0.05 or P<0.01), the content of MDA was significantly decreased(P<0.05), and only T-SOD activity of broilers in HCP-1 group was significantly increased(P<0.01), the activities of CAT showed no significant difference among all groups (P>0.05). 3) The spleen index of broilers in HCP-2 group was significantly increased compared with HCP-0 group (P<0.05), while there was no significant difference among other groups (P>0.05), and there was no significant difference in bursa of fabricius index and thymus index among all groups (P>0.05). 4) On the 21st day of experiment, compared with HCP-0 group, the content of IgG in serum of broilers in HCP-2 group was significantly increased (P<0.05), and the content of IgA in serum of broilers in HCP-1 group, HCP-2 group and HCP-3 group were significantly increased (P<0.05); At the 42nd day of the experiment, the content of IgG in the serum of broilers in HCP-2 group and HCP-3 group was extremely significantly increased compared with HCP-0 group and HCP-1 group (P<0.01), there was no significant difference between HCP-1 group and HCP-0 group(P>0.05); At the 21st and 42nd day of the experiment, the content of IgM in serum of broilers showed no significant difference among all groups (P>0.05). Combined with the above results, dietary HCP supplementation can significantly improve ADG, antioxidant activity and immune function of broilers, and the appropriate amount of HCP added to the feed of broilers was 514-679 mg·kg^-1by regression equation analysis under this experimental conditions.

Key words: Hyriopsis cumingii polysaccharides, growth performance, antioxidant index, imunoglobulin, broiler

CLC Number:

S816.7

XIA Lunbin, MA Longlong, QIAO Deliang, HE Yanfei, JIANG Ping. Effects of Hyriopsis cumingii polysaccharides on growth performance, antioxidant activity and immune function in growing broiler[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 547-555.

Figures/Tables 5

References 30

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项目 Items	含量Content
项目 Items	1~3周 1 to 3 weeks		4~6周 4 to 6 weeks
原料Ingredients
玉米Corn	57.51	59.90
鱼粉Fish meal	3.45	3.00
豆粕Soybean meal	31.50	29.00
豆油Soybean oil	2.80	3.60
石粉Limestone	1.30	1.25
食盐NaCl	0.35	0.35
氯化胆碱Choline chloride	0.10	0.10
L-赖氨酸盐酸盐L-Lys·HCl	0.27	0.28
DL-蛋氨酸DL-Met	0.22	0.22
磷酸氢钙CaHPO₄	1.75	1.55
预混料Premix¹⁾	0.75	0.75
合计Total	100.00	100.00
营养水平Nutrient levels²⁾
代谢能ME/(MJ·kg^-1)	12.47	13.86
粗蛋白CP²⁾	19.54	18.56
赖氨酸Lys	1.21	1.19
蛋氨酸Met	0.62	0.60
钙Ca	1.02	0.96
有效磷AP	0.48	0.48

项目 Items	含量Content
项目 Items	1~3周 1 to 3 weeks		4~6周 4 to 6 weeks
原料Ingredients
玉米Corn	57.51	59.90
鱼粉Fish meal	3.45	3.00
豆粕Soybean meal	31.50	29.00
豆油Soybean oil	2.80	3.60
石粉Limestone	1.30	1.25
食盐NaCl	0.35	0.35
氯化胆碱Choline chloride	0.10	0.10
L-赖氨酸盐酸盐L-Lys·HCl	0.27	0.28
DL-蛋氨酸DL-Met	0.22	0.22
磷酸氢钙CaHPO₄	1.75	1.55
预混料Premix¹⁾	0.75	0.75
合计Total	100.00	100.00
营养水平Nutrient levels²⁾
代谢能ME/(MJ·kg^-1)	12.47	13.86
粗蛋白CP²⁾	19.54	18.56
赖氨酸Lys	1.21	1.19
蛋氨酸Met	0.62	0.60
钙Ca	1.02	0.96
有效磷AP	0.48	0.48

指标 Index	组别Groups				P值P value
指标 Index	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
平均日增重ADG/g	55.45±1.30 B	56.26±0.94 B	57.78±0.87 A	57.74±0.73 A	0.001	<0.001	0.001
平均日采食量ADFI/g	100.72±3.64	102.32±4.41	103.93±3.38	103.47±2.98	0.445	0.191	0.262
料重比F/G	1.82±0.07	1.82±0.08	1.80±0.06	1.79±0.05	0.877	0.445	0.752

指标 Index	组别Groups				P值P value
指标 Index	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
平均日增重ADG/g	55.45±1.30 B	56.26±0.94 B	57.78±0.87 A	57.74±0.73 A	0.001	<0.001	0.001
平均日采食量ADFI/g	100.72±3.64	102.32±4.41	103.93±3.38	103.47±2.98	0.445	0.191	0.262
料重比F/G	1.82±0.07	1.82±0.08	1.80±0.06	1.79±0.05	0.877	0.445	0.752

指标 Index	组别Groups				P值P value
指标 Index	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
总抗氧化能力	16.75±0.55 b	17.12±0.74 b	17.92±0.73 a	17.84±0.47 a	0.012	0.006	0.006
T-AOC/(U·mL^-1)
过氧化氢酶	0.81±0.05	0.82±0.04	0.86±0.06	0.85±0.05	0.307	0.169	0.261
CAT/(U·mL^-1)
总超氧化物歧化酶	114.74±6.85 B	134.01±7.56 A	139.67±8.51 A	136.67±5.33 A	<0.001	0.002	<0.001
T-SOD/(U·mL^-1)
谷胱甘肽过氧化物酶	1274.45±52.16 B	1296.80±84.94 B	1395.82±46.06 A	1384.99±32.19 A	0.002	0.002	0.003
GSH-Px/(U·mL^-1)
丙二醛	9.72±0.43 a	9.52±0.33 ab	8.99±0.44 b	9.05±0.42 b	0.014	0.007	0.009
MDA/(nmol·mL^-1)

Effects of Hyriopsis cumingii polysaccharides on growth performance, antioxidant activity and immune function in growing broiler

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指标 Index	时间 Time	组别Groups				P值P value
指标 Index	时间 Time	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
免疫球蛋白G	21 d	3.83±0.22 b	4.14±0.27 ab	4.43±0.38 a	4.22±0.27 ab	0.015	0.052	0.006
IgG/(g·L^-1)	42 d	5.27±0.38 B	5.60±0.62 B	6.82±0.52 A	6.76±0.51 A	<0.001	<0.001	<0.001
免疫球蛋白A	21 d	0.38±0.08 b	0.52±0.08 a	0.56±0.10 a	0.54±0.10 a	0.012	0.027	0.004
IgA/(g·L^-1)	42 d	0.51±0.04	0.54±0.06	0.62±0.05	0.57±0.04	0.074	0.164	0.054
免疫球蛋白M	21 d	2.10±0.17	2.19±0.16	2.18±0.19	2.15±0.16	0.913	0.801	0.780
IgM/(g·L^-1)	42 d	1.34±0.12	1.41±0.13	1.54±0.17	1.54±0.18	0.316	0.085	0.175

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指标 Index	组别Groups				P值P value
指标 Index	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
胸腺指数Thymus index	3.12±0.12	3.23±0.13	3.29±0.19	3.33±0.25	0.150	0.027	0.068
脾脏指数Spleen index	1.01±0.12 b	1.04±0.12 ab	1.20±0.14 a	1.11±0.11 ab	0.023	0.076	0.038
法氏囊指数	1.41±0.09	1.46±0.09	1.49±0.12	1.45±0.12	0.484	0.471	0.288
Bursa of fabricius index

指标 Index	组别Groups				P值P value
指标 Index	HCP-0	HCP-1	HCP-2	HCP-3	方差分析 ANOVA	线性 Linear	二次 Quadratic
胸腺指数Thymus index	3.12±0.12	3.23±0.13	3.29±0.19	3.33±0.25	0.150	0.027	0.068
脾脏指数Spleen index	1.01±0.12 b	1.04±0.12 ab	1.20±0.14 a	1.11±0.11 ab	0.023	0.076	0.038
法氏囊指数	1.41±0.09	1.46±0.09	1.49±0.12	1.45±0.12	0.484	0.471	0.288
Bursa of fabricius index