Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig

doi:10.3969/j.issn.1004-1524.2023.03.08

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 556-564.DOI: 10.3969/j.issn.1004-1524.2023.03.08

• Animal Science • Previous Articles Next Articles

Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig

YANG Suan¹(), GUO Xiaojiang², YANG Hongwen², XIONG Li², LI Chen², TAN Yuancheng¹, WANG Chunyuan¹, ZHANG Yiyu¹^,^*()

1. Key Laboratory of Plateau Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China
2. Department of Agriculture and Rural Affairs of Guizhou Province, Guiyang 550001, China

Received:2022-05-07 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

The aim of this study was to investigate the polymorphisms of the prolactin receptor gene (PRLR) and their effects on reproductive performance in Kele pigs, with a view to finding molecular markers for improving reproductive performance in Kele pigs. In this study, SNPs in the exon region of the PRLR gene were screened in 204 Kele pigs and identified by direct PCR product sequencing. At the same time, the correlation between different genotypes and diplotypes of SNPs and seven reproductive traits was analysed by single-factor variance method in SPSS software, and the influence of base variation on the secondary structure of mRNA was analysed by online software. The results showed that g.20655220C>T mutation was detected in the 8th exon of PRLR gene, which caused serine to proline change, which was a missense mutation. Two synonymous mutations g.20648859C>T and g.20648931C>T were detected in the fourth intron. There were three genotypes in all three SNPs, g.20648931C>T was low polymorphism and the other two loci were medium polymorphism. Chi-squared test showed that the genotype distribution of g.20655220C>T loci was in Hardy-Weinberg equilibrium (P>0.05). The genotypes of g.20648859C>T and g.20648931C>T deviated significantly(P<0.01) from Hardy-Weinberg equilibrium; g.20655220C>T locus led to changes in the secondary structure of the PRLR gene mRNA sequence, resulting in decreased stability of the secondary structure of the mRNA; the three SNPs consisted of 5 haplotypes and 9 diplotypes. The dominant haplotype H1 (CCC) frequency was 0.610 and the dominant diplotype H1H1 (CCCCCC) frequency was 0.382. Correlation analysis showed that the average birth weight, weaning litter weight and weaning average weight of CC genotype were significantly(P<0.05) higher than those of CT genotype. CT genotype of g.20648931c>T locus had significantly (P<0.05) higher birth weight, birth litter weight and weaning litter weight than those of CC genotype and TT genotype, and CC genotype had significantly (P<0.05) higher birth weight than that of TT genotype. H1H5 (CTCTCT) was the dominant doublet type in terms of litter size and live litter size, whereas H2H3 (CCTTCT) was the superior doublet type in terms of litter weight and average weaning weight. In conclusion, PRLR gene could be used as a candidate gene for molecular marker-assisted selection of breeding traits in Kele pig.

Key words: PRLR gene, KeLe pig, polymorphism, reproductive traits

CLC Number:

S828

YANG Suan, GUO Xiaojiang, YANG Hongwen, XIONG Li, LI Chen, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 556-564.

Figures/Tables 9

References 30

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引物名称 Primer name	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')	基因位置 Location in gene	产物长度 Product length/bp	退火温度 Tm/℃
P1	ACAGTCACCTCCGGGAAAAC	TTGTTGGGAGACACGCGAAT	g.20640695~g.20640852	158	59
P2	CTGGATCCCGACAGCGATTC	CTTGGATCCATCCGCGTGAA	g.20655030~g.20655245	216	60
P3	CCCTAGGCCACTCACTTGCT	AATTAGTCCTGGGAGGGCTGT	g.20648455~g.20649007	553	58
P4	ATGATCTGGGCAGTGGCTTT	TGCTGGCACTTACCTCCAAC	g.20652417~g.20653041	625	60

引物名称 Primer name	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')	基因位置 Location in gene	产物长度 Product length/bp	退火温度 Tm/℃
P1	ACAGTCACCTCCGGGAAAAC	TTGTTGGGAGACACGCGAAT	g.20640695~g.20640852	158	59
P2	CTGGATCCCGACAGCGATTC	CTTGGATCCATCCGCGTGAA	g.20655030~g.20655245	216	60
P3	CCCTAGGCCACTCACTTGCT	AATTAGTCCTGGGAGGGCTGT	g.20648455~g.20649007	553	58
P4	ATGATCTGGGCAGTGGCTTT	TGCTGGCACTTACCTCCAAC	g.20652417~g.20653041	625	60

SNP位点 SNP loci	基因型(频率,样本数) Genotype(frequency, sample number))			等位基因(频率) Allele(frequency)		He	Ne	PIC	χ²
g.20655220 C>T	CC (0.583,119)	CT (0.358,73)	TT (0.059,12)	C(0.762)	T(0.238)	0.362	1.569	0.297	0.033
g.20648859 C>T	CC (0.657,134)	CT (0.255,52)	TT (0.088,18)	C(0.784)	T(0.216)	0.338	1.511	0.281	12.405
g.20648931 C>T	CC (0.755,154)	CT (0.196,40)	TT (0.049,10)	C(0.853)	T(0.147)	0.251	1.335	0.219	9.730

SNP位点 SNP loci	基因型(频率,样本数) Genotype(frequency, sample number))			等位基因(频率) Allele(frequency)		He	Ne	PIC	χ²
g.20655220 C>T	CC (0.583,119)	CT (0.358,73)	TT (0.059,12)	C(0.762)	T(0.238)	0.362	1.569	0.297	0.033
g.20648859 C>T	CC (0.657,134)	CT (0.255,52)	TT (0.088,18)	C(0.784)	T(0.216)	0.338	1.511	0.281	12.405
g.20648931 C>T	CC (0.755,154)	CT (0.196,40)	TT (0.049,10)	C(0.853)	T(0.147)	0.251	1.335	0.219	9.730

SNP位点 SNP loci	g.20655220 C>T	g.20648859 C>T	g.20648931 C>T
g.20655220 C>T		0.111	0.022
g.20648859 C>T	0.001		1.000
g.20648931 C>T	0	0.627

Correlation of PRLR gene polymorphisms and reproductive traits in Kele pig

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倍型 Ploidy	SNP位点(样本数) SNP locus(Sample number)	g.20655220 C>T	g.20648859 C>T	g.20648931 C>T	频率 Frequency/%
单倍型	H1(243)	C	C	C	0.610
Haplotypes	H2(28)	C	T	C	0.069
	H3(40)	C	T	T	0.098
	H4(77)	T	C	C	0.189
	H5(20)	T	T	T	0.049
双倍型	H1H1(78)	C C	C C	C C	0.382
Diplotypes	H1H2(11)	C C	C T	C C	0.054
	H1H3(22)	C C	C T	C T	0.108
	H1H4(44)	C T	C C	C C	0.216
	H1H5(10)	C T	T T	T T	0.049
	H2H3(8)	T T	T T	T T	0.039
	H2H4(9)	C T	C T	C C	0.044
	H3H5(10)	C T	T T	T T	0.049
	H4H4(12)	T T	C C	C C	0.059

位点 Locus	基因型 Genotype	每窝总产仔数 Total number born	每窝产活仔数 Number born alive	初生窝重 Newborn litter weight/kg	初生个体重 Individual birth weight/kg	每窝断奶仔猪数 Number of weaned piglets	断奶窝重 Weight of weaning litter/kg	断奶个体重 Weaning individual weight/kg
g.20655220	CC(119)	8.765±2.547	8.050±2.587	8.785±3.018	1.109±0.229 a	6.967±2.278	39.551±13.000 a	5.776±1.277 a
C>T	CT(73)	8.671±2.582	7.726±2.864	7.950±3.090	1.044±0.205 b	6.616±2.542	35.423±13.395 b	5.417±0.817 b
	TT(12)	9.500±2.468	8.417±3.089	9.283±4.229	1.086±0.209 ab	6.833±2.362	36.600±10.525 ab	5.443±0.589 ab
g.20648859	CC(134)	8.716±2.530	7.948±2.640	8.407±2.993	1.073±0.214	6.828±2.306	37.490±12.621	5.567±0.961
C>T	CT(52)	8.769±2.478	8.019±2.797	8.927±3.417	1.125±0.233	6.846±2.321	38.747±13.351	5.724±1.295
	TT(18)	9.222±2.981	7.833±3.130	8.131±3.416	1.053±0.231	6.833±2.975	38.508±16.323	5.809±1.545
g.20648931	CC(154)	8.617±2.487	7.857±2.631	8.327±2.987 b	1.076±0.221 b	6.747±2.288	37.144±12.757 b	5.570±1.010
C>T	CT(40)	9.225±2.636	8.425±2.782	9.684±3.440 a	1.158±0.207 a	7.300±2.3345	42.326±13.089 a	5.930±1.466
	TT(10)	9.400±3.098	7.600±3.658	6.740±2.970 b	0.916±0.145 c	6.300±3.401	31.845±15.107 b	5.311±0.781

双倍型 Diplotype	每窝总产仔数 Total number born	每窝产活仔数 Number born alive	初生窝重 Newborn litter weight/kg	初生个体重 Individual birth weight/kg	每窝断奶仔猪数 Number of weaned piglets	断奶窝重 Weight of weaning litter/kg	断奶个体重 Weaning individual weight/kg
H1H1(78)	8.731±2.567 ab	8.051±2.613	8.586±2.980 abc	1.084±0.223 abc	6.936±2.275	38.482±12.672 abc	5.635±1.095 ab
H1H2(11)	8.364±2.248 ab	7.364±2.942	7.809±3.263 abc	1.091±0.310 abc	6.091±2.300	35.455±14.903 abc	5.836±1.527 ab
H1H3(22)	9.000±2.582 ab	8.364±2.441	9.587±2.859 a	1.165±0.204 a	7.318±2.255	42.739±11.863 ab	6.011±1.397 ab
H1H4(44)	8.477±2.492 ab	7.636±2.589	7.853±2.576 bc	1.050±0.201 bc	6.636±2.441	35.973±13.129 bc	5.480±0.775 b
H1H5(10)	9.900±2.601 a	8.800±3.795	9.750±4.875 ab	1.091±0.217 abc	7.100±2.726	37.810±14.445 abc	5.351±0.934 b
H2H3(8)	9.000±3.024 ab	8.125±2.532	9.869±3.284 ab	1.224±0.206 a	7.500±2.390	46.838±14.531 a	6.433±2.054 a
H2H4(9)	7.444±1.944 b	7.111±2.147	7.767±2.763 abc	1.108±0.238 abc	6.333±2.062	34.056±13.041 b	5.302±1.029 b
H3H5(10)	9.400±3.098 ab	7.600±3.658	6.740±2.969 b	0.916±0.145 b	6.300±3.41	31.845±15.103 bc	5.311±0.781 b
H4H4(12)	9.500±2.468 ab	8.417±3.088	9.283±4.229 abc	1.086±0.210 abc	6.833±2.125	36.600±10.525 bc	5.443±0.589 ab

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