Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck

doi:10.3969/j.issn.1004-1524.20231237

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (11): 2456-2464.DOI: 10.3969/j.issn.1004-1524.20231237

• Animal Science • Previous Articles Next Articles

Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck

DONG Biao¹(), JI Rongchao², ZHANG Gansheng¹, WANG Jian¹^,²

1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, Jiangsu, China
2. National Gene Bank of Waterfowl Resources, Taizhou 225300, Jiangsu, China

Received:2023-10-30 Online:2024-11-25 Published:2024-11-27

Abstract

Abstract:

The study was to investigate the distribution of single nucleotide in the A-FABP gene exon 2 in black Muscovy duck, and the correlation between these single nucleotide and performance. The polymorphism of exon 2 of A-FABP gene was detected by PCR-SSCP technique in black Muscovy duck. The genetic parameters of this population and the polymorphism with growth performance and meat quality were analyzed, so as to provide reference for molecular marker-assisted selection. The result indicated that there was A-C mutation in exon 2 of A-FABP gene about black Muscovy duck, which was synonymous mutation. Three genotypes, AA, AB and BB, were formed with genotype frequencies of 0.098 8, 0.401 2 and 0.500 0, A and B allele frequencies of 0.299 4 and 0.700 6, respectively. The homozygosity, heterozygosity and polymorphic information content (PIC) was 0.580 5, 0.419 5 and 0.331 5, respectively. The birth weight of AA male duck was significantly higher than that of AB and BB male duck. The abdominal fat weight of AA male duck was significantly higher than that of AB and BB, and the stomachus muscularis weight of AA male duck was significantly higher than that of BB. The percentage of half-eviscerated yield of AB and BB was significantly higher than that of AA, the abdominal fat weight of AA was significantly higher than that of BB, and the percentage of abdominal fat of AA was significantly higher than that of AB and BB in female duck. The pH value in breast muscle of AA male duck was significantly higher than that of AB and BB. The content of inosinic acid in breast muscle of AA and AB males was significantly higher than that of BB males, and the content of Asp in AB males was significantly higher than that of AA males. The content of Glu in breast muscle of BB female duck was significantly higher than that of AA type. The content of Glu and Gly in leg muscle of AA male duck was significantly higher than that of AB and BB. The content of Gly in leg muscle of AA female ducks was significantly higher than that of AB and BB, and the content of Thr and Val in leg muscle of AA female ducks was significantly higher than that of BB. Therefore, A-C mutation in exon 2 of A-FABP gene can be used to assist the selection of abdominal fat weight and partial amino acid content in muscle of black Muscovy duck.

Key words: black Muscovy duck, A-FABP gene, polymorphism, growth performance, meat quality

CLC Number:

S834.89

DONG Biao, JI Rongchao, ZHANG Gansheng, WANG Jian. Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck[J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2456-2464.

Figures/Tables 7

References 19

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指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	10	41	45	6	24	36
初生Birth	52.7±4.43 a	49.5±3.91 b	49.6±4.79 b	50.1±1.14 ab	48.5±4.01 b	48.7±3.03 b
2周龄2 weeks old	328.6±21.56 a	327.0±34.13 a	321.6±35.18 ab	284.8±38.12 c	300.5±29.20 bc	294.7±27.52 c
4周龄4 weeks old	801.3±97.26 a	828.1±90.34 a	799.9±92.32 a	631.7±67.77 b	628.9±74.74 b	638.6±69.18 b
6周龄6 weeks old	1 545.8±167.36 a	1 594.8±179.08 a	1 562.6±172.05 a	1 170.7±102.13 b	1 167.0±121.03 b	1 201.3±124.86 b
8周龄8 weeks old	2 191.5±244.06 a	2 208.3±254.48 a	2 160.6±254.81 a	1 502.7±152.83 b	1 534.5±153.28 b	1 565.9±132.27 b
10周龄10 weeks old	2 800.6±312.10 a	2 714.4±298.34 a	2 720.1±330.71 a	1 756.2±175.05 b	1 767.0±166.49 b	1 798.7±125.61 b
13周龄13 weeks old	3 204.2±281.05 a	3 126.8±339.22 a	3 095.3±333.92 a	1 872.3±174.40 b	1 877.2±189.34 b	1908.3±153.83 b

指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	10	41	45	6	24	36
初生Birth	52.7±4.43 a	49.5±3.91 b	49.6±4.79 b	50.1±1.14 ab	48.5±4.01 b	48.7±3.03 b
2周龄2 weeks old	328.6±21.56 a	327.0±34.13 a	321.6±35.18 ab	284.8±38.12 c	300.5±29.20 bc	294.7±27.52 c
4周龄4 weeks old	801.3±97.26 a	828.1±90.34 a	799.9±92.32 a	631.7±67.77 b	628.9±74.74 b	638.6±69.18 b
6周龄6 weeks old	1 545.8±167.36 a	1 594.8±179.08 a	1 562.6±172.05 a	1 170.7±102.13 b	1 167.0±121.03 b	1 201.3±124.86 b
8周龄8 weeks old	2 191.5±244.06 a	2 208.3±254.48 a	2 160.6±254.81 a	1 502.7±152.83 b	1 534.5±153.28 b	1 565.9±132.27 b
10周龄10 weeks old	2 800.6±312.10 a	2 714.4±298.34 a	2 720.1±330.71 a	1 756.2±175.05 b	1 767.0±166.49 b	1 798.7±125.61 b
13周龄13 weeks old	3 204.2±281.05 a	3 126.8±339.22 a	3 095.3±333.92 a	1 872.3±174.40 b	1 877.2±189.34 b	1908.3±153.83 b

指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	7	29	34	6	22	33
宰前活重 Slaughter weight/g	3 269.7± 228.19 a	3 143.8± 281.70 a	3 129.9± 353.05 a	1 866.0± 188.19 b	1 885.9± 200.92 b	1931.9± 141.43 b
屠体重 Dressed weight/g	3 021.6± 219.59 a	2 883.5± 262.05 a	2 870.5± 309.71 a	1 683.8± 182.27 b	1 703.4± 186.81 b	1 742.8± 139.35 b
屠宰率 Dressed percentage/%	92.40± 1.24 a	91.78± 3.62 a	91.80± 2.82 a	90.19± 1.87 a	90.32± 1.97 a	90.20± 2.64 a
半净膛重 Half-eviscerated weight/g	2 811.3± 200.77 a	2 693.6± 265.51 a	2 662.6± 301.76 a	1 495.3± 175.67 b	1 557.7± 166.18 b	1 603.3± 141.76 b
半净膛率 Percentage of half-eviscerated yield/%	85.98± 0.65 a	85.65± 2.82 a	85.10± 2.14 a	80.10± 3.83 c	82.65± 2.61 b	82.95± 2.88 b
全净膛重 Eviscerated weight/g	2 590.9± 197.64 a	2 507.7± 251.90 a	2 467.1± 276.12 a	1 421.6± 237.15 b	1 427.0± 158.77 b	1 456.4± 126.07 b
全净膛率 Percentage of eviscerated yield/%	79.22± 1.04 a	79.79± 4.34 a	78.87± 2.47 a	75.82± 5.88 b	75.76± 4.21 b	75. 37± 2.92 b
胸肌重 Breast muscle weight/g	471.0± 59.71 a	444.3± 59.87 a	443.1± 71.31 a	268.3± 41.66 b	262.5± 36.06 b	268.7± 30.48 b
胸肌率 Percentage of breast muscle/%	18.13± 1.17 a	17.68± 1.28 a	17.93± 1.64 a	18.92± 1.05 a	18.40± 1.44 a	18.46± 1.46 a
腿肌重 Leg muscle weight/g	301.1± 39.13 a	299.1± 37.81 a	287.5± 41.65 a	137.0± 11.56 b	146.0± 15.59 b	148.2± 15.70 b
腿肌率 Percentage of leg muscle/%	11.62± 1.19 a	11.99± 1.57 a	11.68± 1.45 a	9.76± 0.96 b	10.27± 0.85 b	10.20± 0.93 b
腹脂重 Abdominal fat weight/g	47.46± 4.49 a	42.43± 4.62 bc	41.56± 6.38 bc	46.40± 6.12 ab	42.35± 6.51 bc	39.81± 6.05 c
腹脂率 Percentage of abdominal fat/%	1.83± 0.16 c	1.70± 0.20 c	1.69± 0.24 c	3.31± 0.47 a	2.97± 0.38 b	2.74± 0.38 b
肝重 Liver weight/g	63.1± 13.3 a	61.8± 13.73 a	59.4± 12.54 a	35.3± 3.66 b	33.4± 5.89 b	34.0± 9.11 b
腺肌胃重 Stomachus muscularis weight/g	80.7± 11.29 a	73.4± 12.65 ab	71.1± 13.75 b	55.0± 5.22 c	54.8± 6.47 c	56.4± 9.76 c
心重 Heart weight/g	26.2± 4.19 a	27.0± 7.11 a	24.9± 4.03 a	17.3± 2.02 b	16.7± 3.11 b	15.7± 3.07 b

指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	7	29	34	6	22	33
宰前活重 Slaughter weight/g	3 269.7± 228.19 a	3 143.8± 281.70 a	3 129.9± 353.05 a	1 866.0± 188.19 b	1 885.9± 200.92 b	1931.9± 141.43 b
屠体重 Dressed weight/g	3 021.6± 219.59 a	2 883.5± 262.05 a	2 870.5± 309.71 a	1 683.8± 182.27 b	1 703.4± 186.81 b	1 742.8± 139.35 b
屠宰率 Dressed percentage/%	92.40± 1.24 a	91.78± 3.62 a	91.80± 2.82 a	90.19± 1.87 a	90.32± 1.97 a	90.20± 2.64 a
半净膛重 Half-eviscerated weight/g	2 811.3± 200.77 a	2 693.6± 265.51 a	2 662.6± 301.76 a	1 495.3± 175.67 b	1 557.7± 166.18 b	1 603.3± 141.76 b
半净膛率 Percentage of half-eviscerated yield/%	85.98± 0.65 a	85.65± 2.82 a	85.10± 2.14 a	80.10± 3.83 c	82.65± 2.61 b	82.95± 2.88 b
全净膛重 Eviscerated weight/g	2 590.9± 197.64 a	2 507.7± 251.90 a	2 467.1± 276.12 a	1 421.6± 237.15 b	1 427.0± 158.77 b	1 456.4± 126.07 b
全净膛率 Percentage of eviscerated yield/%	79.22± 1.04 a	79.79± 4.34 a	78.87± 2.47 a	75.82± 5.88 b	75.76± 4.21 b	75. 37± 2.92 b
胸肌重 Breast muscle weight/g	471.0± 59.71 a	444.3± 59.87 a	443.1± 71.31 a	268.3± 41.66 b	262.5± 36.06 b	268.7± 30.48 b
胸肌率 Percentage of breast muscle/%	18.13± 1.17 a	17.68± 1.28 a	17.93± 1.64 a	18.92± 1.05 a	18.40± 1.44 a	18.46± 1.46 a
腿肌重 Leg muscle weight/g	301.1± 39.13 a	299.1± 37.81 a	287.5± 41.65 a	137.0± 11.56 b	146.0± 15.59 b	148.2± 15.70 b
腿肌率 Percentage of leg muscle/%	11.62± 1.19 a	11.99± 1.57 a	11.68± 1.45 a	9.76± 0.96 b	10.27± 0.85 b	10.20± 0.93 b
腹脂重 Abdominal fat weight/g	47.46± 4.49 a	42.43± 4.62 bc	41.56± 6.38 bc	46.40± 6.12 ab	42.35± 6.51 bc	39.81± 6.05 c
腹脂率 Percentage of abdominal fat/%	1.83± 0.16 c	1.70± 0.20 c	1.69± 0.24 c	3.31± 0.47 a	2.97± 0.38 b	2.74± 0.38 b
肝重 Liver weight/g	63.1± 13.3 a	61.8± 13.73 a	59.4± 12.54 a	35.3± 3.66 b	33.4± 5.89 b	34.0± 9.11 b
腺肌胃重 Stomachus muscularis weight/g	80.7± 11.29 a	73.4± 12.65 ab	71.1± 13.75 b	55.0± 5.22 c	54.8± 6.47 c	56.4± 9.76 c
心重 Heart weight/g	26.2± 4.19 a	27.0± 7.11 a	24.9± 4.03 a	17.3± 2.02 b	16.7± 3.11 b	15.7± 3.07 b

指标 Index		公鸭Male duck			母鸭Female duck
指标 Index		AA	AB	BB	AA	AB	BB
样本量Sample size		7	29	34	6	22	33
胸肌 Breast muscle	酸碱度 pH value	6.41± 0.78 a	5.93± 0.25 b	6.05± 0.16 b	5.99± 0.24 b	5.98± 0.17 b	5.96± 0.18 b
	剪切力 Shearing force/N	29.55± 6.62 ab	30.85± 5.65 a	31.80± 5.92 a	25.53± 4.43 ab	29.61± 5.94 ab	27.55± 6.20 b
	失水率 Rate of water loss/%	35.92± 2.84 a	36.58± 3.99 a	37.96± 4.31 a	36.17± 3.24 a	37.84± 2.69 a	36.26± 4.26 a
腿肌 Leg muscle	酸碱度 pH value	6.14± 0.27 a	6.29± 0.39 a	6.23± 0.22 a	6.25± 0.20 a	6.25± 0.12 a	6.26± 0.17 a
	剪切力 Shearing force/N	31.55± 4.53 ab	33.67± 4.86 ab	34.98± 6.48 a	29.18± 4.70 b	33.07± 6.49 ab	32.54± 5.96 ab
	失水率 Rate of water loss/%	34.72± 5.05 a	34.28± 4.28 a	32.57± 5.15 ab	28.45± 6.45 c	30.08± 3.36 bc	29.51± 4.53 bc

Study on correlation of polymorphism of A-FABP gene exon 2 with growth performance and meat quality in Muscovy duck

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指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	4	17	21	4	15	23
肌苷酸	3.66±0.36 a	3.66±0.60 a	2.90±0.59 b	3.20±0.19 ab	3.05±0.56 ab	3.00±0.65 b
Inosinic acid/(mg·g^-1)
天冬氨酸Asp/%	2.81±0.82 c	3.88±0.62 ab	3.50±0.79 bc	4.65±1.21 a	3.91±1.00 ab	4.20±0.93 ab
丝氨酸Ser/%	1.87±0.08 a	2.34±0.82 a	2.20±0.34 a	2.49±1.17 a	1.96±0.75 a	1.96±0.35 a
谷氨酸Glu/%	6.50±0.71 ab	6.84±1.03 ab	6.67±1.13 ab	6.18±0.24 b	7.00±1.03 ab	7.46±1.06 a
甘氨酸Gly/%	1.39±0.22 b	1.68±0.37 ab	1.66±0.38 ab	1.84±0.48 a	1.90±0.44 a	1.70±0.35 ab
组氨酸His/%	1.18±0.42 a	1.17±0.34 a	1.15±0.30 a	1.28±0.42 a	1.03±0.25 a	0.93±0.32 a
精氨酸Arg/%	3.54±0.79 a	3.45±0.76 a	3.36±0.64 a	3.56±1.02 a	3.68±0.72 a	3.90±0.51 a
苏氨酸Thr/%	2.44±0.46 a	2.36±0.51 a	2.40±0.51 a	1.59±0.58 b	1.51±0.47 b	1.81±0.45 b
丙氨酸Ala/%	1.80±0.42 c	2.09±0.53 abc	2.00±0.51 bc	2.34±0.36 ab	2.43±0.40 ab	2.52±0.30 a
脯氨酸Pro/%	1.80±0.50 a	2.07±0.41 a	2.05±0.32 a	1.97±0.20 a	2.06±0.37 a	2.18±0.34 a
半胱氨酸Cys/%	0.41±0.04 a	0.40±0.09 a	0.40±0.09 a	0.39±0.06 a	0.39±0.09 a	0.38±0.08 a
色氨酸Tyr/%	1.88±0.55 b	2.05±0.32 ab	1.98±0.42 b	2.38±0.33 a	2.12±0.33 ab	2.21±0.29 ab
缬氨酸Val/%	2.21±0.41 c	2.60±0.47 abc	2.36±0.45 bc	2.68±0.42 ab	2.70±0.41 ab	2.85±0.33 a
蛋氨酸Met/%	4.66±0.67 a	4.20±0.67 ab	3.99±0.77 ab	3.53±0.32 b	3.53±0.53 b	3.71±0.67 b
赖氨酸Lys/%	3.18±0.49 c	3.48±0.48 bc	3.36±0.58 bc	3.54±0.76 abc	3.85±0.80 ab	4.05±0.50 a
异亮氨酸Ile/%	2.37±0.28 b	2.57±0.44 ab	2.57±0.25 ab	2.64±0.38 ab	2.65±0.33 ab	2.76±0.21 a
亮氨酸Leu/%	4.27±0.55 a	4.37±0.66 a	4.44±0.49 a	4.57±0.11 a	4.56±0.51 a	4.81±0.49 a
苯丙氨酸Phe/%	2.69±0.35 b	3.06±0.43 ab	2.80±0.39 ab	3.09±0.27 a	2.80±0.26 ab	2.86±0.26 ab

[1]	LI Fei, SU Tiantian, SU Kangjie, XU Ke, MA Li, LIU Ziming. Effects of Spirulina platensis and Haematococcus pluvialis on the growth performance, antioxidant enzymes, phosphatase, and heat shock protein of zebrafish (Danio rerio) [J]. Acta Agriculturae Zhejiangensis, 2024, 36(7): 1511-1518.
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[3]	XIANG Jin, WANG Chunyuan, WU Yan, TAN Yuancheng, YANG Suan, ZHANG Yiyu. CRISP3 gene SNP identification and its impact on reproductive traits in Kela pigs [J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1270-1278.
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[5]	YUAN Ye, LIU Rui, WANG Lingyun, SHEN Meng, YE Xuelian, QUAN Xinhua, WANG Ruisen, YAO Xiangtan. Genetic diversity analysis of Trapa L. cultivars in Jiangsu and Zhejiang Provinces using SLAF-seq [J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1773-1781.
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指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	4	17	21	4	15	23
肌苷酸	2.71±1.10 a	2.96±0.82 a	2.72±0.66 a	3.11±0.78 a	3.30±0.55 a	3.27±0.54 a
Inosinic acid/(mg·g^-1)
天冬氨酸Asp/%	4.04±0.57 a	3.72±0.67 ab	3.56±0.88 ab	3.37±1.04 ab	2.94±0.73 b	3.00±0.70 b
丝氨酸Ser/%	1.92±0.28 a	1.89±0.39 a	2.32±0.59 a	2.19±0.63 a	2.13±0.41 a	2.05±0.36 a
谷氨酸Glu/%	7.92±1.35 a	6.79±1.00 b	6.88±0.90 b	5.47±1.46 c	6.04±1.09 bc	6.25±0.93 bc
甘氨酸Gly/%	2.01±0.37 a	1.62±0.22 b	1.57±0.33 b	2.01±0.25 a	1.64±0.35 b	1.49±0.28 b
组氨酸His/%	1.29±0.50 a	1.29±0.41 a	1.17±0.34 a	1.47±0.39 a	1.32±0.38 a	1.27±0.34 a
精氨酸Arg/%	3.31±0.58 b	3.20±0.55 b	3.11±0.58 b	3.92±0.11 a	3.55±0.50 ab	3.43±0.47 ab
苏氨酸Thr/%	1.70±0.38 ab	1.90±0.55 ab	1.89±0.49 ab	2.14±0.88 a	1.77±0.59 ab	1.55±0.46 b
丙氨酸Ala/%	2.37±0.18 a	2.20±0.47 a	2.13±0.38 a	2.40±0.20 a	2.28±0.36 a	2.17±0.43 a
脯氨酸Pro/%	2.08±0.35 a	2.05±0.27 a	2.02±0.42 a	2.15±0.23 a	2.13±0.24 a	2.03±0.20 a
半胱氨酸Cys/%	0.29±0.02 abc	0.33±0.07 a	0.32±0.05 ab	0.23±0.02 c	0.25±0.07 bc	0.25±0.07 c
色氨酸Tyr/%	2.28±0.20 ab	2.18±0.29 abc	2.31±0.22 a	2.12±0.11 abc	2.02±0.31 bc	2.00±0.27 c
缬氨酸Val/%	2.70±0.30 ab	2.59±0.31 ab	2. 65±0.33 ab	2.83±0.11 a	2.58±0.31 ab	2.39±0.51 b
蛋氨酸Met/%	3.84±0.27 a	3.67±0.44 ab	3.82±0.61 a	3.09±0.66 b	3.53±0.64 ab	3.49±0.72 ab
赖氨酸Lys/%	4.13±0.13 a	3.95±0.45 ab	3.69±0.57 ab	4.09±0.27 ab	3.78±0.41 ab	3.64±0.37 b
异亮氨酸Ile/%	2.65±0.34 a	2.57±0.35 a	2.50±0.46 a	2.72±0.18 a	2.55±0.46 a	2.42±0.46 a
亮氨酸Leu/%	4.49±0.50 ab	4.36±0.46 ab	4.01±0.63 b	4.73±0.26 a	4.53±0.48 ab	4.39±0.41 ab
苯丙氨酸Phe/%	2.46±0.31 b	2.50±0.35 b	2.39±0.37 b	2.88±0.12 a	2.58±0.28 ab	2.67±0.18 ab

指标 Index	公鸭Male duck			母鸭Female duck
指标 Index	AA	AB	BB	AA	AB	BB
样本量Sample size	4	17	21	4	15	23
肌苷酸	2.71±1.10 a	2.96±0.82 a	2.72±0.66 a	3.11±0.78 a	3.30±0.55 a	3.27±0.54 a
Inosinic acid/(mg·g^-1)
天冬氨酸Asp/%	4.04±0.57 a	3.72±0.67 ab	3.56±0.88 ab	3.37±1.04 ab	2.94±0.73 b	3.00±0.70 b
丝氨酸Ser/%	1.92±0.28 a	1.89±0.39 a	2.32±0.59 a	2.19±0.63 a	2.13±0.41 a	2.05±0.36 a
谷氨酸Glu/%	7.92±1.35 a	6.79±1.00 b	6.88±0.90 b	5.47±1.46 c	6.04±1.09 bc	6.25±0.93 bc
甘氨酸Gly/%	2.01±0.37 a	1.62±0.22 b	1.57±0.33 b	2.01±0.25 a	1.64±0.35 b	1.49±0.28 b
组氨酸His/%	1.29±0.50 a	1.29±0.41 a	1.17±0.34 a	1.47±0.39 a	1.32±0.38 a	1.27±0.34 a
精氨酸Arg/%	3.31±0.58 b	3.20±0.55 b	3.11±0.58 b	3.92±0.11 a	3.55±0.50 ab	3.43±0.47 ab
苏氨酸Thr/%	1.70±0.38 ab	1.90±0.55 ab	1.89±0.49 ab	2.14±0.88 a	1.77±0.59 ab	1.55±0.46 b
丙氨酸Ala/%	2.37±0.18 a	2.20±0.47 a	2.13±0.38 a	2.40±0.20 a	2.28±0.36 a	2.17±0.43 a
脯氨酸Pro/%	2.08±0.35 a	2.05±0.27 a	2.02±0.42 a	2.15±0.23 a	2.13±0.24 a	2.03±0.20 a
半胱氨酸Cys/%	0.29±0.02 abc	0.33±0.07 a	0.32±0.05 ab	0.23±0.02 c	0.25±0.07 bc	0.25±0.07 c
色氨酸Tyr/%	2.28±0.20 ab	2.18±0.29 abc	2.31±0.22 a	2.12±0.11 abc	2.02±0.31 bc	2.00±0.27 c
缬氨酸Val/%	2.70±0.30 ab	2.59±0.31 ab	2. 65±0.33 ab	2.83±0.11 a	2.58±0.31 ab	2.39±0.51 b
蛋氨酸Met/%	3.84±0.27 a	3.67±0.44 ab	3.82±0.61 a	3.09±0.66 b	3.53±0.64 ab	3.49±0.72 ab
赖氨酸Lys/%	4.13±0.13 a	3.95±0.45 ab	3.69±0.57 ab	4.09±0.27 ab	3.78±0.41 ab	3.64±0.37 b
异亮氨酸Ile/%	2.65±0.34 a	2.57±0.35 a	2.50±0.46 a	2.72±0.18 a	2.55±0.46 a	2.42±0.46 a
亮氨酸Leu/%	4.49±0.50 ab	4.36±0.46 ab	4.01±0.63 b	4.73±0.26 a	4.53±0.48 ab	4.39±0.41 ab
苯丙氨酸Phe/%	2.46±0.31 b	2.50±0.35 b	2.39±0.37 b	2.88±0.12 a	2.58±0.28 ab	2.67±0.18 ab