Association of FGF5 and COQ9 gene polymorphism and growth traits in Tan sheep

doi:10.3969/j.issn.1004-1524.20230736

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1015-1023.DOI: 10.3969/j.issn.1004-1524.20230736

• Animal Science • Previous Articles Next Articles

Association of FGF5 and COQ9 gene polymorphism and growth traits in Tan sheep

MA Li’na¹(), TIAN Jinyang², WANG Jin¹, ZHAO Zhengwei¹, MA Qing¹^,^*()

1. Institute of Animal Sciences, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China
2. Tan Sheep Breeding Farm in Ningxia Hui Autonomous Region, Wuzhong 751102, Ningxia, China

Received:2023-06-07 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

To explore the polymorphisms of fibroblast growth factor 5 (FGF5) and coenzyme Q9 (COQ9) genes and their associations with growth traits in Tan sheep fibroblast growth factor (FGF5) and coenzyme Q9 (COQ9), so as to provide a theoretical basis for the use of molecular marker-assisted breeding methods to improve the growth traits of Tan sheep, the polymorphisms of FGF5 and COQ9 genes were detected in 107 Tan sheep using SNP typing technology for the loci screened in the previous study, and the body weight of primary birth, 35 days old, 6 months old and 12 months old was analyzed. The results showed that two SNPs were screened on the FGF5 gene of Tan sheep by alignment with the reference genome of sheep (GCF_000298735.2_Oar_v4.0), which were g. 94540629 T>G and g. 94540642 T>C, where g. Three genotypes, TT, GT and GG, were produced g.94540629 T>G, and three genotypes of TT, TC and CC were generated at the g.94540642 T>C loci. Three SNPs were screened in the COQ9 gene, which were g. 94503480 A>G, g. 24807700 A>G and g. 24812228 T>C, where three genotypes of AA, AG and GG were produced at the g.94503480 A>G loci, three genotypes of AA, AG and GG3 were generated at the g.24807700 A>G loci, and three genotypes of TT, TC and CC were generated at the g.24812228 T>C loci. Gene polymorphism analysis showed that the five SNPs loci were highly polymorphic and in the Hardy-Weinberg equilibrium state in the Tan sheep population (P>0.05). The results of association analysis showed that the weight of the g.94540642 T>C locus of FGF5 gene C/T genotype at 12 months was significantly greater than that of other genotypes (P<0.05), the body weight of GG genotype at the g.24807700 A>G locus of COQ9 gene was significantly greater than that of other genotypes at 12 months (P<0.05), and the body weight of g.24812228 T>C loci C/C genotype at 12 months was significantly greater than that of other genotypes (P<0.05), it was concluded that the three SNP loci had a significant effect on the body weight of Tan sheep at 12 months, the COQ9 gene g. 24812228 T>C and g.24807700 A>G loci were in strong linkage imbalance in the Tan sheep population, the FGF5 gene g. 94540629 T>G and g. 94540642 T>C loci were in strong linkage imbalance in the Tan sheep population, and the COQ9 gene g. 94503480 A>G, g. 24807700 A>G and g. 24812228 T>C locus genotypes combination A/GA/AT/T and A/GA/GT/C were significantly higher than those of other combination genotypes at birth weight, 6 months weight and 12 months weight (P<0.05). FGF5 and COQ9 gene polymorphisms were significantly correlated with the growth traits of Tan sheep, and the FGF5 and COQ9 genes could be used as candidate genes for the breeding of growth traits and molecular markers of weight traits in Tan sheep, and the breeding efficiency of Tan sheep could be improved by conventional breeding combined with molecular marker-assisted selection.

Key words: Tan sheep, FGF5, COQ9, gene polymorphisms, growth trait, association analysis

CLC Number:

S826

MA Li’na, TIAN Jinyang, WANG Jin, ZHAO Zhengwei, MA Qing. Association of FGF5 and COQ9 gene polymorphism and growth traits in Tan sheep[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1015-1023.

Figures/Tables 9

References 21

[1]	苏鹏, 黄洋铭, 兰天鑫, 等. 绵羊生长性状关键基因挖掘研究进展[J]. 家畜生态学报, 2021, 42(9): 1-8.
	SU P, HUANG Y M, LAN T X, et al. Research progress on key genes mining of growth traits in sheep[J]. Journal of Domestic Animal Ecology, 2021, 42(9): 1-8. (in Chinese with English abstract)
[2]	王燕新, 廖圆圆, 阿依木古丽, 等. 三个绵羊品种LHX3基因多态性与生长性状的关联分析[J]. 生物技术通报, 2019, 35(10): 162-168.
	WANG Y X, LIAO Y Y, A Y, et al. Correlation analysis between LHX3 gene polymorphism and growth traits in three sheep breeds[J]. Biotechnology Bulletin, 2019, 35(10): 162-168. (in Chinese with English abstract)
[3]	白俊艳, 曹恒, 王旭, 等. 绵羊GH基因的PvuⅡ位点的多态性与其生长性状的关联分析[J]. 浙江农业学报, 2019, 31(9): 1416-1422.
	BAI J Y, CAO H, WANG X, et al. Association between the polymorphism of PvuⅡ locus of GH gene and growth traits in sheep[J]. Acta Agriculturae Zhejiangensis, 2019, 31(9): 1416-1422. (in Chinese with English abstract)
[4]	安清明, 周辉通, 王星, 等. UCP1基因变异与绵羊生长及胴体性状的关联分析[J]. 中国畜牧兽医, 2019, 46(11): 3289-3298.
	AN Q M, ZHOU H T, WANG X, et al. Association analysis of UCP1 gene variation with growth and carcass traits in ovine[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(11): 3289-3298. (in Chinese with English abstract)
[5]	李文杨, 刘远, 吴贤锋, 等. 山羊IGF-1基因的骨骼肌表达特性及其SNPs与生长性状的关联分析[J]. 农业生物技术学报, 2019, 27(12): 2188-2197.
	LI W Y, LIU Y, WU X F, et al. Skeletal muscle expression characteristics of IGF-1 gene and its SNPs association with growth traits in goat(Capra hircus)[J]. Journal of Agricultural Biotechnology, 2019, 27(12): 2188-2197. (in Chinese with English abstract)
[6]	李海健, 王燕新, 廖圆圆, 等. 不同绵羊品种GHSR基因多态性与生长性状的关联分析[J]. 现代畜牧兽医, 2020(1): 21-26.
	LI H J, WANG Y X, LIAO Y Y, et al. Association analysis between GHSR gene polymorphism and growth traits in different sheep breeds[J]. Modern Journal of Animal Husbandry and Veterinary Medicine, 2020(1): 21-26. (in Chinese with English abstract)
[7]	张天闻, 张稳, 邹诗凡, 等. 绵羊生长素释放肽基因的多态性及与生长性状的关联分析[J]. 基因组学与应用生物学, 2021, 40(S4): 3404-3417.
	ZHANG T W, ZHANG W, ZOU S F, et al. Polymorphism and its association analysis with growth traits of sheep GHRL gene[J]. Genomics and Applied Biology, 2021, 40(S4): 3404-3417. (in Chinese with English abstract)
[8]	马丽娜, 刘永进, 马青, 等. 滩羊C1H3orf33和CNV14基因拷贝数变异与生长性状的关联研究[J]. 中国畜牧杂志, 2023, 59(3): 123-130.
	MA L N, LIU Y J, MA Q, et al. Correlation between copy number variation of C1H3orf33 and CNV14 genes and growth traits in Tan sheep[J]. Chinese Journal of Animal Science, 2023, 59(3): 123-130. (in Chinese)
[9]	WIGGINTON J E, CUTLER D J, ABECASIS G R. A note on exact tests of Hardy-Weinberg equilibrium[J]. American Journal of Human Genetics, 2005, 76(5): 887-893.
[10]	ZHOU X, STEPHENS M. Genome-wide efficient mixed-model analysis for association studies[J]. Nature Genetics, 2012, 44(7): 821-824.
[11]	BARRETT J C, FRY B, MALLER J, et al. Haploview: analysis and visualization of LD and haplotype maps[J]. Bioinformatics, 2005, 21(2): 263-265.
[12]	蒙亚琦, 姚旭东, 任秀美奥, 等. 利用CRISPR/Cas9n系统编辑绵羊成纤维细胞生长因子5(FGF5)基因[J]. 畜牧与兽医, 2022, 54(1): 8-15.
	MENG Y Q, YAO X D, REN X, et al. Editing the FGF5 gene in the fibroblasts of Ovis aries using the CRISPR/Cas9n system[J]. Animal Husbandry & Veterinary Medicine, 2022, 54(1): 8-15. (in Chinese with English abstract)
[13]	王妞. FGF5和FGF21影响绒山羊毛乳头细胞增殖及其调控机制研究[D]. 杨凌: 西北农林科技大学, 2022.
	WANG N. Study on the proliferation and regulation mechanism of FGF5 and FGF21 in dermal papilla cells in cashmere goats[D]. Yangling: Northwest A & F University, 2022. (in Chinese with English abstract)
[14]	胡慧宇, 任思睿, 刘晨曦, 等. FGF5基因编辑细毛羊的健康状况评估[J]. 草食家畜, 2021(5): 9-18.
	HU H Y, REN S R, LIU C X, et al. Health evaluation of FGF5 gene-modified merino sheep[J]. Grass-Feeding Livestock, 2021(5): 9-18. (in Chinese with English abstract)
[15]	CHEN B, HU R, MIN Q, et al. FGF5 regulates schwann cell migration and adhesion[J]. Frontiers in Cellular Neuroscience, 2020, 14: 237.
[16]	CHEN M M, ZHAO Y, LI Y, et al. Reproduction and viscera organ characteristics of MSTN and FGF5 dual-gene knockout sheep[J]. Frontiers in Veterinary Science, 2023, 10: 1119312.
[17]	CHEN M M, LIAN D, LI Y, et al. Global long noncoding RNA expression profiling of MSTN and FGF5 double-knockout sheep reveals the key gatekeepers of skeletal muscle development[J]. DNA and Cell Biology, 2023, 42(3): 163-175.
[18]	SONG T Z, TAN Y, CUOMU R Q, et al. Polymorphisms and mRNA expression levels of IGF-1, FGF5, and KAP 1.4 in Tibetan Cashmere goats[J]. Genes, 2023, 14(3): 711.
[19]	蒋妍. 植物辅酶Q合成途径脂结合蛋白COQ9的研究[D]. 上海: 上海师范大学, 2021.
	JIANG Y. Lipid-binding protein COQ9 in plant coenzyme Q biosynthetic pathway[D]. Shanghai: Shanghai Normal University, 2021. (in Chinese with English abstract)
[20]	毕亚珍, 尚明玉, 胡文萍, 等. 绵羊生长性状间的相关和回归分析及TRHDE基因多态性与生长性状的关联分析[J]. 畜牧兽医学报, 2023, 54(4): 1415-1428.
	BI Y Z, SHANG M Y, HU W P, et al. Correlation and regression analysis among growth traits and association analysis of TRHDE gene polymorphism with growth traits in sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428. (in Chinese with English abstract)
[21]	孟科, 张天闻, 梁鹏, 等. 绵羊MYF5基因多态性及其与生长性状的关联分析[J]. 农业生物技术学报, 2022, 30(3): 496-505.
	MENG K, ZHANG T W, LIANG P, et al. Polymorphism of MYF5 gene and its association analysis with growth traits in sheep(Ovis aries)[J]. Journal of Agricultural Biotechnology, 2022, 30(3): 496-505. (in Chinese with English abstract)

基因名称 Gene name	位点名称 Site name	Chr	Pos.	Ref	Alt	引物序列 Primer sequence(5'-3')	退火温度 Annealing temperature/℃	片段长度 Fragment length/bp	Func.ref gene
Fibroblast growth factor 5 (FGF5)	chr6_94540629	6	94540629	G	T	F:CAGAATCTCTTACCCCTCCTGC R:CAGGTCTTAATTGCAGCATGTG	58	246	UTR
	chr6_94540642	6	94540642	C	T	F:CGATCCAGGGATTGAACCAG R:TCCATGTGCCAGGTCTTAATTG	58	272	UTR
Coenzyme Q9 (COQ9)	chr6_94503480	6	94503480	A	G	F:GGTACGTGGCCCTGAACAAG R:GTTAGGACTTCTCCGAGGTGC	58	209	CDS
	chr14_24807700	14	24807700	G	A	F:GTCCTCGTGGATTACAGTTTGG R:TGTCAGTATCAGTGTGTGAGTGCC	58	207	intron
	chr14_24812228	14	24812228	C	T	F:CCCCAGGGTGACAGTGTGTTC R:GCCACTCACATCTCCAGGAAC	58	202	intron

基因名称 Gene name	位点名称 Site name	Chr	Pos.	Ref	Alt	引物序列 Primer sequence(5'-3')	退火温度 Annealing temperature/℃	片段长度 Fragment length/bp	Func.ref gene
Fibroblast growth factor 5 (FGF5)	chr6_94540629	6	94540629	G	T	F:CAGAATCTCTTACCCCTCCTGC R:CAGGTCTTAATTGCAGCATGTG	58	246	UTR
	chr6_94540642	6	94540642	C	T	F:CGATCCAGGGATTGAACCAG R:TCCATGTGCCAGGTCTTAATTG	58	272	UTR
Coenzyme Q9 (COQ9)	chr6_94503480	6	94503480	A	G	F:GGTACGTGGCCCTGAACAAG R:GTTAGGACTTCTCCGAGGTGC	58	209	CDS
	chr14_24807700	14	24807700	G	A	F:GTCCTCGTGGATTACAGTTTGG R:TGTCAGTATCAGTGTGTGAGTGCC	58	207	intron
	chr14_24812228	14	24812228	C	T	F:CCCCAGGGTGACAGTGTGTTC R:GCCACTCACATCTCCAGGAAC	58	202	intron

位点编号 Loci number	位点名称 Loci name	基因型 Genotype	基因频率 gene frequency	等位基因 Frequencies	频率 Allelic gene	纯合度 Ho	杂合度 He	有效等位基因数 Ne	多态信息含量 PIC	P
SNP1	chr6:94503480	AA(70)	0.65	A	0.82	0.70	0.30	1.43	0.25	1.0
		AG(32)	0.30	G	0.18
		GG(3)	0.03
SNP2	chr6:94540629	TT(73)	0.68	T	0.81	0.75	0.25	1.34	0.25	0.20
		TG(28)	0.26	G	0.19
		GG(6)	0.06
SNP3	chr6: 94540642	TT(73)	0.68	T	0.81	0.75	0.25	1.34	0.25	0.11
		TC(28)	0.26	C	0.19
		CC(6)	0.06
SNP4	chr14:24807700	AA(30)	0.28	A	0.55	0.49	0.51	2.06	0.37	0.85
		AG(56)	0.52	G	0.45
		GG(21)	0.20
SNP5	chr14:24812228	TT(30)	0.28	T	0.55	0.49	0.51	2.06	0.37	0.85
		TC(56)	0.52	C	0.45
		CC(21)	0.20

位点编号 Loci number	位点名称 Loci name	基因型 Genotype	基因频率 gene frequency	等位基因 Frequencies	频率 Allelic gene	纯合度 Ho	杂合度 He	有效等位基因数 Ne	多态信息含量 PIC	P
SNP1	chr6:94503480	AA(70)	0.65	A	0.82	0.70	0.30	1.43	0.25	1.0
		AG(32)	0.30	G	0.18
		GG(3)	0.03
SNP2	chr6:94540629	TT(73)	0.68	T	0.81	0.75	0.25	1.34	0.25	0.20
		TG(28)	0.26	G	0.19
		GG(6)	0.06
SNP3	chr6: 94540642	TT(73)	0.68	T	0.81	0.75	0.25	1.34	0.25	0.11
		TC(28)	0.26	C	0.19
		CC(6)	0.06
SNP4	chr14:24807700	AA(30)	0.28	A	0.55	0.49	0.51	2.06	0.37	0.85
		AG(56)	0.52	G	0.45
		GG(21)	0.20
SNP5	chr14:24812228	TT(30)	0.28	T	0.55	0.49	0.51	2.06	0.37	0.85
		TC(56)	0.52	C	0.45
		CC(21)	0.20

位点编号 Loci number	位点名称 Loci name	基因型 Genotype	出生重 Birth weight/kg	二毛重 35-day weight/kg	6月体重 6-month weight/kg	12月体重 12-month weight/kg
SNP1	chr6:94503480	A/A	5.45±0.07	16.21±0.29	32.78±0.67	44.25±0.80
		A/G	5.32±0.11	15.29±0.44	31.25±1.01	45.47±1.20
		G/G	5.66±0.38	18.73±1.44	32.86±3.32	44.00±3.94
SNP2	chr6:94540629	T/T	5.33±0.09	15.96±0.36	32.40±0.82	45.32±0.96
		T/G	5.43±0.09	16.07±0.36	32.28±0.81	43.73±0.95
		G/G	5.55±0.27	15.63±1.05	32.10±2.37	45.66±2.78
SNP3	chr6: 94540642	C/C	5.44±0.09	16.04±0.35	32.44±0.80	43.89±0.94 b
		C/T	5.31±0.12	15.71±0.49	31.90±1.11	47.17±1.30 a
		T/T	5.47±0.12	16.20±0.48	32.53±1.09	44.44±1.28 b
SNP4	chr14:24807700	A/A	5.50±0.12	16.48±0.45	32.57±0.99	44.66±1.21 b
		A/G	5.35±0.08	15.44±0.33	31.02±0.75	43.85±0.92 b
		G/G	5.47±1.13	16.75±0.54	37.39±1.21	48.51±1.48 a
SNP5	chr14:24812228	T/T	5.50±0.11	16.48±0.45	32.57±0.99	44.66±1.21 b
		T/C	5.35±0.08	15.44±0.33	31.02±0.75	43.85±0.91 b
		C/C	5.47±0.15	16.75±0.55	36.39±1.21	49.22±1.48 a

Association of FGF5 and COQ9 gene polymorphism and growth traits in Tan sheep

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基因型 Combination genotype	出生重 Birth weight	二毛重 35-day weight	6月体重 6-month weight	12月体重 12-month weight
T/TT/T(85)	5.65±0.49	16.51±1.17	36.03±0.53	49.05±1.28
G/GC/C(16)	5.94±0.31	17.63±1.44	38.93±1.34	49.66±1.68
T/GT/C(15)	5.86±0.58	17.05±1.41	34.89±1.32	46.71±1.94

基因型 Combination genotype	出生重 Birth weight	二毛重 35-day weight	6月体重 6-month weight	12月体重 12-month weight
A/AA/GT/C(35)	5.23±0.33 b	16.34±1.09	36.76±0.23 b	48.75±1.34 b
A/AG/GC/C(6)	5.43±0.12 b	16.53±1.21	37.11±1.32 b	47.76±1.19 b
A/GA/AT/T(10)	5.70±0.26 a	17.77±1.91	39.89±1.09 a	49.43±1.24 a
A/AA/AT/T/C(20)	5.45±0.34 b	16.44±1.29	36.30±0.27 b	48.56±1.53 b
A/GA/GT/C(21)	5.84±0.29 a	17.63±1.32	39.95±1.02 a	49.73±1.92 a
A/AG/GC/C(8)	5.36±0.38 b	16.67±1.34	35.21±1.56 b	45.39±1.09 b

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