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    2024, 36(5):  0-0. 
    Abstract ( 90 )   PDF (25273KB) ( 343 )  
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    Crop Science
    Genome-wide association analysis of barley grain related traits
    LUAN Haiye, ZHU Linjie, LI Yu, MENG Wei, LIU Yuqian, XU Xiao, LIU Fangfang, SHEN Huiquan
    2024, 36(5):  997-1002.  DOI: 10.3969/j.issn.1004-1524.20230754
    Abstract ( 319 )   HTML ( 402 )   PDF (923KB) ( 512 )  

    Grain size and thousand grain weight is the main factors that determine the barley yield and uses. It is of great importance to mine candidate genes related to grain for improving the yield and quality of barley. In this study, genome-wide association study (GWAS) was carried out to detect the candidate genes associated with grain traits in natural population containing 242 barley germplasm resources. The results showed that these traits showed rich phenotypic variation, with the coefficient of variation ranged from 6.65%-19.98%. Thousand grain weight was significantly correlated with grain length and grain width. The correlation coefficient between thousand grain weight and grain width was appreciably higher than thousand grain weight and grain length. Fifty-two SNP locus were detected by GWAS on 7 chromosomes. BLAST analysis showed that six candidate genes related to grain traits were selected. The ethylene response factor on chromosome 7 was simultaneously correlated with grain width and thousand grain weight, indicating that there was a multi-effect function of this gene. The results of this study provide new genetic resources and theoretical basis for the genetic improvement of barley yield and quality.

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    Effects of plant growth regulators on yield, quality and photosynthetic characteristics of high-quality japonica rice
    ZHAO Liming, WANG Yaxin, JIANG Wenxin, DUAN Shaobiao, SHEN Xuefeng, ZHENG Dianfeng, FENG Naijie
    2024, 36(5):  1003-1014.  DOI: 10.3969/j.issn.1004-1524.20230798
    Abstract ( 276 )   HTML ( 42 )   PDF (1232KB) ( 563 )  

    In order to explore the effects of plant growth regulator on photosynthetic characteristics, yield and quality of high-quality japonica rice, the experimental materials were selected as the Suijing 18, Kendao 12, and Sanjiang 6, and eleven plant growth regulators including abscisic acid (ABA), 6-benzylaminoadenine (6-BA), diethyl aminoethyl caproate (DA-6), spermidine (Spd), 3-indolebutyric acid (IBA), 3,5-dinitrosalicylic acid (SA), brassinolide (BL), zeatin (ZT), triacontanol (TRIA), methyl jasmonate (MeJA), and N6-furan methyl adenine (KT) were sprayed at the flag leaf expansion stage, in order to analyze the effects of plant growth regulator on yield, quality and photosynthetic characteristics of rice. The results showed spraying BL increased dry matter accumulation and stem-sheath matter transport after heading, increased grain weight per panicle, 1 000-grain weight, seed setting rate, and grain-grass ratio, with an average increase of 7.61% in two years, compared with control (CK). Spraying 6-BA could improve the photosynthetic characteristics after heading, prolong the duration of green leaf area from heading stage to waxy stage, increase the number of grains per panicle, grain weight per panicle, 1 000-grain weight, and grain-grass ratio, with an average increase of 6.40% in two years. However, the yield increase effect of DA-6, ZT, ABA, Spd, IBA, SA, and KT were different between years and varieties. In terms of quality, spraying DA-6 and ZT increased milled rice rate and head rice rate, and improved rice appearance quality. According to the comprehensive analysis, the application of BL and 6-BA in the development period of sword leaf is suitable for increasing the yield of test varieties, while the application of DA-6 and ZT is more suitable for improving the quality under the premise of stable yield or increasing the yield.

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    Animal Science
    Association of FGF5 and COQ9 gene polymorphism and growth traits in Tan sheep
    MA Li’na, TIAN Jinyang, WANG Jin, ZHAO Zhengwei, MA Qing
    2024, 36(5):  1015-1023.  DOI: 10.3969/j.issn.1004-1524.20230736
    Abstract ( 168 )   HTML ( 7 )   PDF (1566KB) ( 382 )  

    To explore the polymorphisms of fibroblast growth factor 5 (FGF5) and coenzyme Q9 (COQ9) genes and their associations with growth traits in Tan sheep fibroblast growth factor (FGF5) and coenzyme Q9 (COQ9), so as to provide a theoretical basis for the use of molecular marker-assisted breeding methods to improve the growth traits of Tan sheep, the polymorphisms of FGF5 and COQ9 genes were detected in 107 Tan sheep using SNP typing technology for the loci screened in the previous study, and the body weight of primary birth, 35 days old, 6 months old and 12 months old was analyzed. The results showed that two SNPs were screened on the FGF5 gene of Tan sheep by alignment with the reference genome of sheep (GCF_000298735.2_Oar_v4.0), which were g. 94540629 T>G and g. 94540642 T>C, where g. Three genotypes, TT, GT and GG, were produced g.94540629 T>G, and three genotypes of TT, TC and CC were generated at the g.94540642 T>C loci. Three SNPs were screened in the COQ9 gene, which were g. 94503480 A>G, g. 24807700 A>G and g. 24812228 T>C, where three genotypes of AA, AG and GG were produced at the g.94503480 A>G loci, three genotypes of AA, AG and GG3 were generated at the g.24807700 A>G loci, and three genotypes of TT, TC and CC were generated at the g.24812228 T>C loci. Gene polymorphism analysis showed that the five SNPs loci were highly polymorphic and in the Hardy-Weinberg equilibrium state in the Tan sheep population (P>0.05). The results of association analysis showed that the weight of the g.94540642 T>C locus of FGF5 gene C/T genotype at 12 months was significantly greater than that of other genotypes (P<0.05), the body weight of GG genotype at the g.24807700 A>G locus of COQ9 gene was significantly greater than that of other genotypes at 12 months (P<0.05), and the body weight of g.24812228 T>C loci C/C genotype at 12 months was significantly greater than that of other genotypes (P<0.05), it was concluded that the three SNP loci had a significant effect on the body weight of Tan sheep at 12 months, the COQ9 gene g. 24812228 T>C and g.24807700 A>G loci were in strong linkage imbalance in the Tan sheep population, the FGF5 gene g. 94540629 T>G and g. 94540642 T>C loci were in strong linkage imbalance in the Tan sheep population, and the COQ9 gene g. 94503480 A>G, g. 24807700 A>G and g. 24812228 T>C locus genotypes combination A/GA/AT/T and A/GA/GT/C were significantly higher than those of other combination genotypes at birth weight, 6 months weight and 12 months weight (P<0.05). FGF5 and COQ9 gene polymorphisms were significantly correlated with the growth traits of Tan sheep, and the FGF5 and COQ9 genes could be used as candidate genes for the breeding of growth traits and molecular markers of weight traits in Tan sheep, and the breeding efficiency of Tan sheep could be improved by conventional breeding combined with molecular marker-assisted selection.

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    Cloning and expression analysis of Runx2b in Culter alburnus
    HE Changxi, ZHENG Jianbo, MA Jianbo, JIA Yongyi, LIU Shili, JIANG Wenping, CHI Meili, CHENG Shun, LI Fei
    2024, 36(5):  1024-1031.  DOI: 10.3969/j.issn.1004-1524.20230755
    Abstract ( 174 )   HTML ( 8 )   PDF (4643KB) ( 398 )  

    In order to explore the preliminary function of Runx2b gene of Culter alburnus during the ossification process of intermuscular bones (IBs), we obtained the full-length coding sequence of Runx2b gene by transcriptome data and PCR verification. In this study, we staged the different periods of IBs formation by alizarin red staining, and spatial and temporal expression level of Runx2b were investigated by qRT-PCR. The results showed that the open reading frame (ORF) of Runx2b was 1 401 bp, which encoded 466 amino acids with a predicted molecular weight of 50.676 ku and a theoretical isoelectric point of 9.35. The results of amino acid multi-alignment showed that the topmouth culter Runx2b protein displayed a higher identity of 95% with other cyprinidae fish specie, and shared highly conserved Runt and RunxI domains. Phylogenetic tree analysis showed that the topmouth culter Runx2b was most closely related to that of Megalobrama amblycephala. Alizarin red staining showed that the fins and axial skeleton had developed completely before IBs formation. A few IBs of small length emerged in the tail at 15 d, and had a mature morphology and length at 30 d. In addition, mRNA expression abundance was detected in all tested tissues, with the highest expression level in the gill, followed by muscle. Moreover, the expression pattern of Runx2b gene at different growth stages of juveniles was highly consistent with the timing of IBs ossification, indicating its regulation role in IBs development. Our data will contribute to generating IBs-free new varieties and revealing the mechanism of IBs formation.

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    The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety
    LIAO Pengfei, LI Qiongyan, LUO Shungao, LIU Min, ZHU Hongtao, LI Jiya, BAI Hongying, CHEN Haiquan, FAN Yonghui, DONG Zhanpeng
    2024, 36(5):  1032-1041.  DOI: 10.3969/j.issn.1004-1524.20230794
    Abstract ( 132 )   HTML ( 8 )   PDF (2441KB) ( 362 )  

    To identify the gender of the larvae in the early developmental stages of the current plain silkworm variety Jingsong, for the purpose of achieving sex-separation in rearing and differential utilization, the female of the sex-limited normal marking silkworm race Yuncan 7A was crossed with the male of multilunar silkworm race Cb. Then, these hybrids were respectively crossed with the commercial plain silkworm race Jingsong A and Jingsong B. The progeny were backcrossed to the commercial plain silkworm race for several times and then selfed, combined with categorized selection for individual and batches. This process introduced sex-limited normal marking gene of Yuncan 7A and multilunar gene of Cb into the commercial plain silkworm race Jingsong, directed breeding resulted in Jing A Chaxian and Jing B Chaxian. The results showed that during the breeding process, the larval spot differentiation turned into easy-to-distinguish multilunar and non-multilunar types by color and shape. The cultivated Jing A Chaxian and Jing B Chaxian larvae displayed clearly different color differentiation of multilunar at the beginning of the 3rd instar, allowing for accurate gender identification. The economic traits of Jing A Chaxian and Jing B Chaxian, such as the larva-pupa rate of 96.91% and 96.40%, the whole cocoon weight of 1.62 g and 1.56 g, the cocoon shell ratio of 23.78% and 23.26%, were slightly different from their recurrent parents, exhibiting characteristics of good health, easy rearing, and high-quality cocoons. This study laid a foundation for creating breeding materials with special traits, the differential utilization of male and female silkworms, and reducing the breeding costs of male silkworms.

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    Identification and capsule serotyping of two strains of Pasteurella multocida isolated from rabbit
    JI Quan’an, XU Xiangfei, BAO Guolian, HUANG Ye̍e, CUI Xuemei, LIU Yan
    2024, 36(5):  1041-1046.  DOI: 10.3969/j.issn.1004-1524.20230705
    Abstract ( 123 )   HTML ( 8 )   PDF (2293KB) ( 332 )  

    To investigate the prevalence of major infectious diseases in rabbit farms in Zhejiang Province, the epidemiological survey was carried out to isolate, identify the serotype of pathogenic bacteria. It was found that respiratory infectious diseases, mainly characterized by sneezing and nasal discharge of serous, mucous or purulent secretions, existed in most rabbit farms. Thus, rabbits with severe respiratory diseases from two farms in Ningbo and Shengzhou were sampled for bacterial isolation and identification, serotyping, and drug sensitivity test. Based on the colonial morphology, characters of gram-staining, specific PCR, and capsule seroptyping, the bacterium were identified as capsule seroptype A Pasteurella multocida, which were named as ZJNB0321 and ZJSZ0322, respectively. The evolutionary tree results showed that the two isolates belonged to the same clade, and were in the same clade as Pasteurella multocida, but they had their own separate clade. The results of the drug sensitivity test indicated that the bacterium showed different drug sensitive characteristics. This study provides scientific basis for rational drug use, and development of effective control measures for rabbit farms.

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    Preparation and identification of monoclonal antibodies against non-structural protein C of Peste des petits ruminants virus
    LI Ju, BI Donglin, YANG Xiaoli, YANG Dongliang, ZHANG Xiaowen, LIU Fangcheng, LI Qiongyi, BAI Jialin
    2024, 36(5):  1047-1054.  DOI: 10.3969/j.issn.1004-1524.20230565
    Abstract ( 136 )   HTML ( 4 )   PDF (1119KB) ( 312 )  

    To prepare monoclonal antibody against non-structural protein C of Peste des petits ruminants virus (PPRV), a 20-amino acids antigen peptide of CRSGKPRGETPGPLLPEIMQ and a 21-amino acid screening antigen peptide of PLRAGERGLAPQAVQHRTLIK were designed and synthesized based on the antigenicity analysis of the amino acid sequence of the polypeptide chain encoded by PPRV C gene. The immunogen and screening antigen were prepared by crosslinking with keyhole limpet hemocyanin (KLH) and biotin carboxyl carrier protein (Biotin), respectively. Five female specific pathogen free (SPF) grade BALB/c mice aged 8 to 12 weeks and weighing about 20 g were given intramuscular injection of immunogen for the first immunization, while the second immunization and the third immunization were given at intervals of 7 d after the first immunization, the rush immunization was given at 21 d after the third immunization, and the blood was collected and serum was separated on the third day after the rush immunization. The titer of serum antibody of one immunized mouse was 1:312 500 and the two mice was 1:62 500 by indirect enzyme linked immunosorbent assay (ELISA) detection. Three mouse spleen cells were fused with myeloma cells SP2/0 by polyethylene glycol (PEG) to produce hybridoma cells. 26 positive lymphohybridoma cells were screened by indirect ELISA, and 46 monoclonal cell lines were screened by clonal culture. Western blot (WB) screened two hybridoma cell lines that could steadily secrete monoclonal antibodies against PPRV C protein. WB and indirect immunofluorescence assay (IFA) showed that the monoclonal antibodies were of good sensitivity and viral reactivity. These results laid a foundation for further elucidating the role of C protein in the life cycle of PPRV, and also provided an effective diagnostic reagent for the detection of peste des petits ruminants (PPR).

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    Horticultural Science
    Development of SNP and InDel markers in radish and their association with phenotypic characters
    LI Yadong, LUO Xiaobo, PENG Xiao, YANG Guangqian, JIN Yueyue, ZU Guidong, TIAN Huan, ZHANG Wanping
    2024, 36(5):  1055-1066.  DOI: 10.3969/j.issn.1004-1524.20230663
    Abstract ( 168 )   HTML ( 4 )   PDF (6873KB) ( 393 )  

    In order to explore the molecular markers associated with significant phenotypic traits of radish, we identified 14 phenotypic data of 60 radish samples, and screened out 29 molecular markers with polymorphism (12 SNP markers and 17 InDel markers) for genetic diversity analysis. The general linear mode (GLM) of TASSEL5.0 software was used for correlation analysis of 14 phenotypic data of radish. The results showed that the number of alleles (Na) of 29 molecular markers ranged from 2 to 3, with an average of 2.14. The frequency of major alleles (MAF) ranged from 0.53 to 0.94, with an average of 0.68. The expected heterozygosity (He) for each marker ranged from 0.12 to 0.93, with an average of 0.63. The polymorphism information content (PIC) values of each locus ranged from 0.11 to 0.37, with an average value of 0.32. Among the 12 phenotypic traits, 17 significantly correlated molecular marker sites (7 SNP markers and 10 InDel markers) were associated (P≤0.01), and the contribution rate was 7.24%-23.25%.

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    Transcriptome analysis and HSP response of pitaya (Selenicereus spp.) under heat stress
    LI Juncheng, DANG Yunzhi, SUN Qingming
    2024, 36(5):  1067-1075.  DOI: 10.3969/j.issn.1004-1524.20230843
    Abstract ( 194 )   HTML ( 15 )   PDF (3113KB) ( 379 )  

    To investigate the changes in gene expression under high temperature stress of pitaya, we studied key genes that respond to such stress and screened heat-resistant gene resources. In this study, RNA-seq was used to sequence pitaya stems treated at 25 ℃ and 40 ℃ for 24 h, and differentially that under were analyzed from the perspective of systems biology. The results showed that after pitaya was subjected to 40 ℃ for 24 h, and 2 408 genes showed significant changes compared with expression relative to 25 ℃, including 165 transcription factors, accounting for 6.7% of the total number of genes; the expression level was up-regulated by 2 to 587 times or down-regulated by 2 to 59 times. Heat shock proteins (HSPs) were particularly up-regulated after stress, and the top 10 up-regulated genes in differentially expressed genes were all HSPs, which may have important stress tolerance effects. By qPCR detection of the expression levels of these 10 HSPs in samples at different stress time, it was found that HSP18.2, HSP21, HSP22, HSP23.5 and HSP23.6 were up-regulated by more than 50 times after stress for 5 h, especially HSP21, which was up-regulated by 391 times after stress for 5 h and reached the highest level after stress for 10 h with an up-regulation of 589 times. The rapid response of HSP may be related to the high temperature tolerance of pitaya. HSP21 can be used as a signal gene for high temperature stress of pitaya.

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    Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate
    HUANG Rong, MENG Qingxin, WU Xiaoman, XUN Lijie, ZHANG Junli, DONG Xia, DONG Kun, GONG Xueyang
    2024, 36(5):  1076-1085.  DOI: 10.3969/j.issn.1004-1524.20230757
    Abstract ( 134 )   HTML ( 12 )   PDF (1572KB) ( 286 )  

    Taking the pedicel of Camellia reticulata as the test material, the community structure and diversity of endophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequencing. The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages, and there were significant differences in species diversity and richness. Ascomycota and Basidiomycota were the dominant phyla (the sum of their abundance>90%) in the pedicels of Camellia reticulata of two tree ages, but the dominant populations of the two groups of samples at the level of order and family were very different. Many species (fungi) can only be classified to the level of order, among which unclassified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large proportion. The function of endophytic fungi was predicted to obtain 11 ecological symbionts, with the highest proportion of unknown. Animal pathogens, plant pathogens, fungal parasites, and various saprophytic bacteria formed a multifunctional nutrient type. The abundance of pathogens, parasites, and saprophytic bacteria in endophytic fungi was greatly affected by tree age. In summary, the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly, and the species, abundance, and function of dominant fungi also changed. Therefore, the age factor cannot be ignored when using endophytic fungi.

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    Plant Protection
    Pathogen identification of root rot of Curcuma wenyujin
    LI Yani, CHEN Weiliang, MAO Bizeng
    2024, 36(5):  1086-1093.  DOI: 10.3969/j.issn.1004-1524.20230797
    Abstract ( 117 )   HTML ( 5 )   PDF (3734KB) ( 281 )  

    At main production areas of Curcuma wenyujin in Taoshan Town, Rui’an City, Zhejiang Province, large-scale lodging of C. wenyujin and severe rot of their underground tubers occurred in some fields. To identify the pathogen that causes the disease, the bacteria strain JF1 was isolated from the rotten tuber of C. wenyujin by using LB and TTC (2,3,5-triphenyltetrazolium chloride) medium. The pathogenicity of isolated strain JF1 was confirmed by using three inoculation methods, including root irrigation, bud infection and rhizome infection. The taxonomic status of pathogenic bacteria was identified according to the morphological characteristics, physiological and biochemical tests, phylogenetic analysis of multiple gene sequences (16S rDNA, rpoB, gyrB). The results showed that the pathogenic bacteria causing root rot of C. wenyujin was Enterobacter mori.

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    Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain
    HE Yushan, ZHU Haixia
    2024, 36(5):  1094-1101.  DOI: 10.3969/j.issn.1004-1524.20231149
    Abstract ( 132 )   HTML ( 4 )   PDF (2302KB) ( 376 )  

    Recently, microbial herbicides have become a research hotspot due to their advantages in safety and environment protection. In order to explore the weeding mechanism of Alternaria gaisen GD-011 strain, experiments were carried out on Elsholtzia densa Benth by observing the ultrastructure changes of E. densa leaves after inoculation of GD-011 strain, as well as determining the changes of physiological and biochemical indexes of leaves. The results showed that the mycelia of strain GD-011 invaded E.densa through stomata to leaf tissue, parasitically reproduced in the tissue, produced spores, and gradually destroyed the tissue. After inoculation of strain GD-011, the malondialdehyde (MDA) content in the leaves of E. densa gradually increased. Meanwhile, the activities of antioxidant enzyme systems, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), increased first and then decreased. The soluble protein content in the leaves of E. densa after inoculation for 2-7 d was significantly (P<0.05) higher than that in the control. In general, it was inferred that the GD-011 strain achieved weeding effect by infecting through stomata, destroying the cell membrane and tissue structure and inhibiting self-repair, etc.

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    Environmental Science
    Effects of two nematicide treatments on soil nematode community and soil properties in Chinese yam field
    FAN Linjuan, WU Caiyun, XU Xueliang, LIU Zirong, YAO Jian, KANG Hongbo, HU Pinghua, YAO Yingjuan
    2024, 36(5):  1102-1112.  DOI: 10.3969/j.issn.1004-1524.20230448
    Abstract ( 137 )   HTML ( 4 )   PDF (1191KB) ( 411 )  

    To investigate the effect of 10% fosthizate granule (GR)+6% oligosaccharide·fosthiazate emulsion in water (EW) (CP1 for short) and 42% metam-sodium aqueous solutions (AS) (CP2 for short) on the soil ecological environment in Chinese yam field, nematode classification methods and conventional soil analysis methods were adopted to study the effect of the above treatments on soil nematode community and soil properties in the Chinese yam field cultivated by directional shallow trough method (DS) and traditional method (TR), respectively. The result showed that, compared with the blank control (CK), these two treatments did not significantly impact the soil moisture content, pH value, organic matter content, yet significantly (P<0.05) increased the available potassium content in the 0-20 cm layer of the soil cultivated by DS and reduced the available potassium content in the>20-40 cm layer of the soil cultivated by TR. Compared with the CK, CP1 treatment had no significant impact on the proportion of bacterivores and herbivores. However, it significantly reduced the relative abundance of Pratylenchus, which was harmful for the Chinese yam production, and increased the relative abundance of Rhabditis and Acrobeloides, which were beneficial for the Chinese yam production in the 0-20,>20-40 cm layer of the soil cultivated by TR. CP2 treatment not only significantly reduced the relative abundance of Pratylenchus in the 0-20 cm layer of the soil cultivated by DS and the 0-20,>20-40 cm layer of the soil cultivated by TR, but also significantly increased the relative abundance of Rhabditis and Acrobeloides in the 0-20,>20-40 cm layer of the soil cultivated by TR than those of the CK. Besides, CP2 treatment significantly reduced the value of PPI/MI (ratio of plant parasite index to maturity index) in the 0-20 cm layer of the soil cultivated by TR, yet increased the Shannon diversity index, Pielou evenness index, Wasilewsha index and nematode maturity index. In summary, the comprehensive application effect of 42% metam-sodium AS was better than that of 10% fosthizate GR+6% oligosaccharide·fosthiazate EW for the perspective of soil nematode. Under the present experiment conditions, the two treatments would not cause adverse effects on the soil ecological environment, which are suitable for promotion in Chinese yam field.

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    Effect of Fenlong tillage on soil characteristics and cabbage yield in dam land
    GAO Hu, MU Xiaoguo, LI Haijun, GAO Fucheng, ZHANG Ying, LI Jianshe, YE Lin
    2024, 36(5):  1113-1123.  DOI: 10.3969/j.issn.1004-1524.20230165
    Abstract ( 131 )   HTML ( 4 )   PDF (976KB) ( 469 )  

    To reveal the effects of Fenlong tillage on soil characteristics and cabbage yield in dam land, six treatments were set up: flat rotary plowing, flat Fenlong 30 cm, flat Fenlong 40 cm, ridge rotary plowing, ridge Fenlong 30 cm, and ridge Fenlong 40 cm. The effects of the 6 treatments on soil physiochemical properties, enzymes activities, cabbage quality and yield were determined. It was shown that compared with the treatment of flat rotary plowing, in the soil layer of 0-20,>20-40 cm, the ridge Fenlong 40 cm treatment significantly (P<0.05) decreased soil bulk density by 4.14%, 11.45%, respectively; increased soil porosity by 2.38, 5.12 percentage points, respectively; decreased soil pH value by 0.25, 0.20, respectively; decreased soil electrical conductivity by 25.98%, 28.20%, respectively; enhanced the contents of soil organic matter (by 25.34%, 34.57%, respectively), alkali-hydrolyzable nitrogen (by 37.89%, 18.57%, respectively), available potassium (by 31.64%, 24.64%, respectively), total nitrogen (by 38.24%, 46.67%, respectively), and total phosphorus (by 44.44%, 72.22%, respectively); increased the activities of soil urease (by 40.06%, 166.97%, respectively), catalase (by 25.92%, 19.23%, respectively), invertase (by 24.01%, 50.84%, respectively), and alkaline phosphatase (by 66.67%, 26.58%, respectively). The nitrate content in cabbage under the treatment of ridge Fenlong 40 cm was significantly decreased than that under the treatment of flat rotary plowing by 23.54%, yet the contents of soluble sugar, vitamin C and soluble protein were significantly increased by 34.42%, 21.69%, 49.39%, respectively, and the leafy head weight per plant and yield were significantly increased by 14.50% and 13.59%, respectively. In conclusion, ridge Fenlong 40 cm was the best tillage method in the experiment conditions, as it could improve the physiochemical properties of soil, enhance the soil nutrients content and the activities of soil enzymes, and improve the quality and yield of cabbage.

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    Isolation of strains with algicidal activity from South Taihu Lake in China and identification of active compounds
    CHEN Yaoyao, LIU Xueping, ZHANG Shaoyong, WANG Jidong, WANG Ruijun, ZHANG Liqin
    2024, 36(5):  1124-1133.  DOI: 10.3969/j.issn.1004-1524.20230541
    Abstract ( 141 )   HTML ( 3 )   PDF (2000KB) ( 366 )  

    In the present study, culturable bacteria were isolated and purified from water samples of south Taihu Lake in four seasons by the dilution-plate method and modified isolation medium. These bacteria were identified using 16S rRNA gene sequence. Bacteria with algicidal activity against Microcystis aeruginosa were screened, and their active compounds were isolated and purified. It was shown that a total of 420 strains were isolated from water samples of south Taihu Lake in four seasons. They were classified into 192 species, 66 genera, 35 families, 20 orders, 9 classes and 4 phyla. Based on the isolated strain number, Flavobacterium, Pseudomonas, Brevundimonas and Bacillus were the majority at the genus level. There were 20 strains identified as potential new taxa by 16S rRNA gene sequence analysis. Seven strains showed obvious inhibitory activity against M. aeruginosa, of which Flavobacterium sp. 1-2-14 exhibited the strongest algicidal activity, as its inhibition rate reached 71.52% after co-cultured for 7 days. By adopting the tracing method of bioactivity, two compounds, cyclo (4-hydroxy-L-Pro-L-Leu) and pterodontriol B were isolated, and their inhibition rates against M. aeruginosa were 56.87%and 67.81% at 48 h, respectively.

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    Comparison of key properties of short-chain polyphosphate fertilizers in market
    KANG Longfei, XU Linfeng, WANG Shirong, JIANG Chengtao, CHU Guixin
    2024, 36(5):  1134-1143.  DOI: 10.3969/j.issn.1004-1524.20230476
    Abstract ( 124 )   HTML ( 2 )   PDF (1603KB) ( 306 )  

    Currently, the quality of short-chain polyphosphate fertilizers varied greatly. In the present study, 10 typical short-chain polyphosphate fertilizer samples were collected from the fertilizer market in China. Main features of these samples, including the contents of total N and total P (based on P2O5), polymerization degree, polymerization rate, solubility, etc, were determined, and the cluster analysis and Mantel test method were employed to compare the differences of these samples. It was shown that the average degree of polymerization was 4.00, and the average deviation between the measured values and the labeled values was 6.38%. The average polymerization rate was 83.8%, and the average deviation between the measured values and the labeled values was 2.59%. The total N content of these samples ranged from 8.6% to 28.7%, and the average deviation between the measured values and the labeled values was 2.59%. The total P content of these samples was 18.0%-64.2%. Mantel test results demonstrated that the total N content, the total P content, the pH value and salinity index were significantly (P<0.05) correlated with polymerization rate, while the total P content, solubility, salt index and the content of biuret were significantly correlated with the degree of polymerization. In conclusion, the quality of short-chain polyphosphate fertilizers in market varied greatly, and the labeled values differed from the determined values of main features in short-chain polyphosphate fertilizers. Thus, it was urgent and necessary to establish a unified measurement system to provide more accurate and comprehensive information for consumers.

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    Food Science
    Study on the extraction processing and antioxidant activities of saponins from Polygonatum cyrtonema Hua roots
    LIU Chenxing, CAO Yan, XIA Qile
    2024, 36(5):  1144-1152.  DOI: 10.3969/j.issn.1004-1524.20230718
    Abstract ( 153 )   HTML ( 11 )   PDF (3199KB) ( 386 )  

    The roots of Polygonatum cyrtonema Hua have long been considered as by-product with low value, and few relevant studies have been reported. This study aims to clarify the desirable extraction process of saponins from the roots of Polygonatum cyrtonema Hua and evaluate the antioxidant activities of the obtained saponins. Response surface analysis presented that the optimum extraction method was 72% ethanol, 1:25 g·mL-1, and 41 min. The relationship between Y (extraction rate) and factor A (ethanol volume fraction), factor B (solid-liquid ratio), factor C (extraction time) was presented as Y=5.58+0.516 2A+0.078 8B+0.05C-0.02AB-0.007 5AC-0.002 5BC-1.38A2-0.439 5B2-0.257C2. The peak of saponins extraction was 5.62%, which is very close to the estimated peak (5.628%). Antioxidant test exhibited that saponins have good antioxidant activities with 0.87 mg·mL-1 IC50 DPPH and 0.68 mg·mL-1 IC50 ABTS. Overall, the saponins in the roots of Polygonatum cyrtonema Hua have considerable potential in use. This study can provide an important reference basis for the comprehensive utilization of Polygonatum cyrtonema Hua, as well as other by-products.

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    Quality safety risk assessment of watermelon industry in Zhejiang Province, China
    CHU Tianfen, LEI Ling, LI Qinfeng, WU Ping, HONG Wenjie, ZHENG Weiran
    2024, 36(5):  1153-1160.  DOI: 10.3969/j.issn.1004-1524.20230596
    Abstract ( 205 )   HTML ( 5 )   PDF (941KB) ( 448 )  

    Pesticide residues in watermelons of Zhejiang Province were analyzed to clarify the types and levels of pesticide residues and evaluate the risk assessment. The results showed that 29 pesticide residues were detected in 288 watermelon samples, three pesticides with high detection rates were pyraclostrobin, acetamiprid and carbendazim. And glyphosate and thiamethoxam were found to exceed the maximum residue limits of China each in one sample, the other detected pesticides met the requirements of standards. The results of single factor pollution indices and acute and chronic dietary exposure risk values proved that the dietary intake risk of watermelons in Zhejiang Province is acceptable, and there is no health risk. What’s more, we should focus on the strict pesticide residue limit of other countries (regions), based on the needs of international trade, to formulate pesticide residue limit standards that meet the actual production of watermelon and protect the health consumers in China. Comparing the difference of domestic and foreign standards to integrate with the international community and improve the competitiveness of China’s watermelon industry.

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    Biosystems Engineering
    Portable famous tea fresh leaves picking machine based on lifting-picking action
    WANG Hongcheng, BAI Zihao, XU Haihao, XU Kai, HUANG Along, WANG Ze’en, WAN Fei, ZHANG Linan, WU Liqun
    2024, 36(5):  1161-1172.  DOI: 10.3969/j.issn.1004-1524.20230614
    Abstract ( 136 )   HTML ( 5 )   PDF (10057KB) ( 368 )  

    To alleviate the current situation where the harvesting of famous teas represented by Longjing heavily relies on manual labor, this paper proposes a fresh leaf harvesting method based on the physical characteristics differences between fresh leaves and old leaves. The method employed purely mechanical elastic structures and flexible harvesting units to achieve the automation of fresh leaf harvesting. First, the study investigated the physical characteristics differences between fresh leaves and old leaves, such as growth height, apparent morphology, and the breaking strength of tea stems, serving as the primary basis for the design of the harvester’s structure and the pulling force parameters. Based on this, inspired by the picking action of tea farmers when manually harvesting fresh leaves, a fresh leaf harvesting mechanism based on the frictional force difference between the upper and lower surfaces of fresh leaves was proposed. The mechanism underwent a dynamics simulation analysis of the harvesting process. According to the simulation results, silica gel and Teflon film were used as the flexible materials in contact with the upper and lower surfaces of fresh leaves, combined with a dynamic bottom plate elastic structure, to provide adjustable pulling force to avoid damage to the fresh leaves during harvesting. Tea garden trial results showed that when the picking force was set to 3.0 N, the harvesting effect was better. The breaking position of the fresh leaf stem depends on the position where the breaking strength of the fresh leaf stem was the minimum, consistent with the breaking position during manual harvesting. The clean picking rate of fresh leaves was about 40%, the mispicking rate of old leaves was about 7%, the tea leaf damage rate was about 5%, and the picking efficiency was about 1.44 kg·h-1 (equivalent to three skilled tea pickers). The results of this study can provide technical accumulation for the fully automatic mechanical harvesting of fresh tea leaves.

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    Design and performance study of leaping-over type soil covering device on film
    LIU Quandong, SUN Wei, ZHANG Hua, LIU Xiaolong, LI Hui, WANG Hucun
    2024, 36(5):  1173-1184.  DOI: 10.3969/j.issn.1004-1524.20230730
    Abstract ( 129 )   HTML ( 7 )   PDF (5163KB) ( 253 )  

    According to the agronomic requirements of potato cultivation with full film mulching and soil covering on film in the cold and dry regions of Northwest China, a leaping-over type soil covering device was designed. The discrete element method (DEM) was used to conduct soil cover simulation experiments. Based on the Box-Behnken experimental design principle, a regression model for soil cover width and soil cover thickness was established using the three factor and three level response surface method. The optimal operating parameter combination for the leaping-over soil covering device was optimized as follows: the rotational speed of the screw conveyor was 135 r·min-1, the opening length of the soil cover was 100 mm, and the height of the soil cover from the ground was 233 mm. Field experiments have shown that the natural seedling emergence rate of potato reached 92.5%, and the seedling burning rate was 7%.

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    Agricultural Economy and Development
    Spatial-temporal characteristics and influencing factors of non-point source pollution of chemical fertilizers in China: based on provincial panel data from 1997 to 2020
    JI Xuanxuan, JIANG Junsong
    2024, 36(5):  1185-1198.  DOI: 10.3969/j.issn.1004-1524.20230699
    Abstract ( 151 )   HTML ( 6 )   PDF (3399KB) ( 236 )  

    The problem of non-point source pollution of chemical fertilizers is related to the green development of agriculture and the revitalization of rural ecology. Based on the provincial panel data from 1997 to 2020, the emission intensity of non-point source pollution of chemical fertilizers was measured. The Kernel density estimation, Markov chain analysis, Theil index, two-way fixed effect model and other methods were adopted to explore the spatial-temporal characteristics and influencing factors of non-point source pollution of chemical fertilizers in China. The results showed that, the emission intensity of non-point source pollution of chemical fertilizers in China was increasing at first and then decreasing. Taking the year of 2015 as the dividing point, the trend of emission intensity of non-point source pollution of chemical fertilizer shifting to a low level gradually appeared, but it was still stable to maintain the original level, and it was difficult to achieve leapfrog reduction. During the sample period, the emission intensity of non-point source pollution of chemical fertilizers showed a spatial distribution pattern of “high in the east and low in the west, high in the south and low in the north”. The intra-regional difference was the main source of the overall difference. Nine factors, such as the level of financial support for agriculture, the level of agricultural economic growth and rural human capital, affected the emission intensity of non-point source pollution of chemical fertilizers. Therefore, it is necessary not only to formulate differentiated pollution control measures, but also to strengthen cooperation in pollution control among provincial administrative regions. Meanwhile, it is important to expand the path of fertilizer reduction and efficiency increase in order to promote greener agricultural development in all respects.

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    Analysis of Genetic Technology (Precision Breeding) Act in UK and its implications for China
    ZHOU Xianjie, CHENG Baoku, ZHANG Wenfei
    2024, 36(5):  1199-1207.  DOI: 10.3969/j.issn.1004-1524.20230724
    Abstract ( 143 )   HTML ( 6 )   PDF (1174KB) ( 304 )  

    Precision breeding technology is important for improving breeding efficiency and agro-environmental benefits by creating high-yielding and high-value-added agricultural products. Based on the pressure on agricultural supply chain and the objective need for new breeding technologies post-Brexit, the Genetic Technology (Precision Breeding) Act in UK was published on March 23, 2023. Stands for a restrictive open policy and sustainable development principle, the act provides differentiated notification procedures and diversified supervision and enforcement mechanisms for the release and sale of precision breeding organisms, and food or feed produced from precision breeding organisms. In the context of rapid development of new breeding technologies, China should establish a supervision program based on product characteristics, provide gene editing labeling and traceability mechanism, and improve biotechnology legislation and law enforcement mechanism to balance the needs of sustainable agricultural development and biosafety.

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    Review
    Research progress in remediation of soil heavy metal pollution by Sphingosinomonas
    LUZI Zhenggang, ZHU Lixin, JI Hongbing, WANG Kang
    2024, 36(5):  1208-1216.  DOI: 10.3969/j.issn.1004-1524.20230938
    Abstract ( 287 )   HTML ( 29 )   PDF (1032KB) ( 526 )  

    Sphingosinomonas is gram-negative bacterium, which could degradate aromatic compounds and polycyclic aromatic hydrocarbons. Works indicated that Sphingosinomonas could degradate heavy metals, and the expression process of related functional genes had been discovered. However, the degradation mechanism and the complete degradation process has not been well elucidated. In this paper, the research status of Sphingosinomonas was summarized, the remediation effects of Sphingosinomonas on heavy metal pollution was reviewed, the influencing factors in the remediation process was evaluated as well as its mechanism of promoting plant growth. In addition, the key genes and mechanisms of Sphingosinomonas in the remediation of heavy-metal contaminated soil were analyzed, which offered ideas and basis for the further development and utilization of Sphingomonas in the remediation of environmental pollution.

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