Association analysis between SSR markers and germination-related traits in sorghum germplasm resources

doi:10.3969/j.issn.1004-1524.2021.06.01

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 965-973.DOI: 10.3969/j.issn.1004-1524.2021.06.01

• Crop Science • Previous Articles Next Articles

Association analysis between SSR markers and germination-related traits in sorghum germplasm resources

LIU Xiuhui(), ZOU Guihua, ZHAI Guowei, LIU Heqin, ZHENG Xueqiang, CHEN Heyun^*()

Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2020-10-21 Online:2021-06-25 Published:2021-06-25
Contact: CHEN Heyun

Abstract

Abstract:

Fifty-four pairs of simple sequence repeat (SSR) markers spanned uniformly over sorghum genome were selected to detect 110 sorghum germplasm resources. The relevances between SSR and germination-related traits (mesocotyl length, coleoptile elongate, root length, and hundred-grain weight) were studied in order to provide theoretical foundation and materials for screening elite deep-seeding tolerance in sorghum. The results showed that there were significantly (P≤0.05) negative correlation between mesocotyl length and root length, and significantly (P≤0.01) positive correlation between mesocotyl length and coleoptile length. Significantly (P≤0.01) positive correlations were found among coleoptile length, root length, and hundred-grain weight. A total of 339 alleles were identified at all of 54 SSR markers loci, with an average of 6.28 alleles per marker locus and 2.67 effective alleles. The average values of Shannon’s information index were 1.129 9. All of the sorghum germplasms were divided into three subgroups by structural analysis. Nine sites had significant (P≤0.05) correlation with mesocotyl length, whose phenotypic contribution rate was from 11.18%-28.05%. Five sites had significant (P≤0.05) correlation with coleoptile elongate, whose phenotypic contribution rate were 11.22%-18.12%. Only one site had significant (P≤0.05) correlation with root length. Two sites had significant correlation with hundred-grain weight.

Key words: sorghum, seedling-emergence related traits, SSR markers, association analysis

CLC Number:

S514

LIU Xiuhui, ZOU Guihua, ZHAI Guowei, LIU Heqin, ZHENG Xueqiang, CHEN Heyun. Association analysis between SSR markers and germination-related traits in sorghum germplasm resources[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 965-973.

Figures/Tables 7

References 23

[1]	邹剑秋, 王艳秋, 柯福来. 高粱产业发展现状及前景展望[J]. 山西农业大学学报(自然科学版), 2020,40(3):2-8.
	ZOU J Q, WANG Y Q, KE F L. Development status and prospect of Sorghum industry in China[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2020,40(3):2-8.(in Chinese with English abstract)
[2]	杨赞林. 高粱不同播种深度试验[J]. 作物学报, 1963(2):217-219.
	YANG Z L. Different sowing depth tests of sorghum[J]. Acta Agronomica Sinica, 1963(2):217-219.(in Chinese)
[3]	邹桂花, 翟国伟, 陶跃之, 等. 高通量筛选适合深播高粱品种的方法:CN105993878B[P]. 2019-02-19.
[4]	钟改荣, 白永新, 孟俊文. 浅谈抗旱耐深播高产玉米新品种的利用与开发[J]. 玉米科学, 1998,6(1):29-31.
	ZHONG G R, BAI Y X, MENG J W. Application and development of new maize varieties with drought resistance, deep-sowing tolerance and higher yield[J]. Maize Sciences, 1998,6(1):29-31.(in Chinese with English abstract)
[5]	董存吉, 王早荣, 孟俊文, 等. 玉米高产原理及增产途径[J]. 山西农业科学, 1990(1):21-23.
	DONG C J, WANG Z R, MENG J W, et al. High yield principle and ways of increasing yield of maize[J]. Journal of Shanxi Agricultural Science, 1990(1):21-23. (in Chinese)
[6]	张光恒, 林建荣, 吴明国, 等. 水稻出苗顶土动力源研究[J]. 中国水稻科学, 2005,19(1):59-62.
	ZHANG G H, LIN J R, WU M G, et al. Analysis on germinating dynamic source of rice (Oryza sativa)[J]. Chinese Journal of Rice Science, 2005,19(1):59-62.(in Chinese with English abstract)
[7]	杜金友, 张桂荣, 蔡爱军, 等. 玉米中胚轴长度与内源激素关系的研究[J]. 玉米科学, 2008,16(3):70-73.
	DU J Y, ZHANG G R, CAI A J, et al. Relationship between the mesocotyl elongation and hormones in maize (Zea mays L.)[J]. Journal of Maize Sciences, 2008,16(3):70-73.(in Chinese with English abstract)
[8]	周德超. 种子萌发出苗过程中胚轴的伸长和胚根的发展[J]. 生物学通报, 1985,20(7):3-4.
	ZHOU D C. Hypocotyl elongation and radicle development during seed germination and emergence[J]. Biology Bulletin, 1985,20(7):3-4. (in Chinese)
[9]	闫凤霞, 常建忠, 梁笃, 等. 不同高粱种质幼苗器官形态与出苗能力关系的研究[J]. 作物杂志, 2015(1):49-52.
	YAN F X, CHANG J Z, LIANG D, et al. Study on relationship between seedling morphology and emerging ability of different Sorghum germplasm[J]. Crops, 2015(1):49-52.(in Chinese with English abstract)
[10]	王荣焕, 王天宇, 黎裕. 关联分析在作物种质资源分子评价中的应用[J]. 植物遗传资源学报, 2007,8(3):366-372.
	WANG R H, WANG T Y, LI Y. Application of association analysis in molecular evaluation of crop germplasm resources[J]. Journal of Plant Genetic Resources, 2007,8(3):366-372.(in Chinese with English abstract)
[11]	AHN E, HU Z B, PERUMAL R, et al. Genome wide association analysis of Sorghum mini core lines regarding anthracnose, downy mildew, and head smut[J]. PLoS One, 2019,14(5):e0216671. DOI URL
[12]	BOYLES R E, COOPER E A, MYERS M T, et al. Genome-wide association studies of grain yield components in diverse Sorghum germplasm[J]. The Plant Genome, 2016, 9(2): plantgenome2015.09.0091.
[13]	UPADHYAYA H D, WANG Y H, SHARMA R, et al. Identification of genetic markers linked to anthracnose resistance in Sorghum using association analysis[J]. Theoretical and Applied Genetics, 2013,126(6):1649-1657. DOI URL
[14]	DOYLE J J T, DOYLE J L. Isolation of Plant DNA from fresh tissue[J]. Focus, 1990,12(1):13-15. DOI URL
[15]	高东, 李锐, 杨木青, 等. 元阳箐口村哈尼梯田水稻品种SSR遗传多样性分析[J]. 分子植物育种, 2011,9(1):1857-1863.
	GAO D, LI R, YANG M Q, et al. Rice landraces’genetic diversity of Hani terrace wetland in Qingkou village of Yuanyang county[J]. Molecular Plant Breeding, 2011,9(1):1857-1863. (in Chinese with English abstract)
[16]	PRITCHARD J K, STEPHENS M, DONNELLY P. Inference of population structure using multilocus genotype data[J]. Genetics, 2000,155(2):945-959. DOI URL
[17]	冯国郡, 叶凯, 李桂英, 等. 新疆甜高粱种质资源遗传多样性的SSR分析[J]. 植物遗传资源学报, 2012,13(4):549-554,561.
	FENG G J, YE K, LI G Y, et al. Simple sequence repeats polymorphism analysis of sweet Sorghum germplasm in Xinjiang[J]. Journal of Plant Genetic Resources, 2012,13(4):549-554,561.(in Chinese with English abstract)
[18]	倪先林, 赵甘霖, 刘天朋, 等. SSR分子标记在糯高粱种质资源遗传多样性分析中的应用[J]. 江苏农业学报, 2015,31(1):16-22.
	NI X L, ZHAO G L, LIU T P, et al. Genetic diversity analysis of glutinous Sorghum germplasm by simple sequence repeat[J]. Jiangsu Journal of Agricultural Sciences, 2015,31(1):16-22.(in Chinese with English abstract)
[19]	李萌, 秦慧彬, 王宇楠, 等. 基于SSR标记的山西高粱地方品种遗传多样性和遗传结构分析[J]. 分子植物育种, 2020,18(18):6068-6076.
	LI M, QIN H B, WANG Y N, et al. Genetic diversity and structure of Sorghum Landrace germplasm in Shanxi based on SSR markers[J]. Molecular Plant Breeding, 2020,18(18):6068-6076.(in Chinese with English abstract)
[20]	赵香娜, 岳美琪, 刘洋, 等. 国内外甜高粱种质遗传多样性的SSR分析[J]. 植物遗传资源学报, 2010,11(4):407-412.
	ZHAO X N, YUE M Q, LIU Y, et al. Genetic diversity of domestic and foreign sweet Sorghum germplasm revealed by SSR markers[J]. Journal of Plant Genetic Resources, 2010,11(4):407-412.(in Chinese with English abstract)
[21]	田承华, 程庆军, 高海燕, 等. 高粱种质资源SSR标记遗传多样性与农艺性状关联分析[J]. 安徽农业科学, 2018,46(32):26-32.
	TIAN C H, CHENG Q J, GAO H Y, et al. Genetic diversity and association analysis using SSR markers and agronomic traits in Sorghum[J]. Journal of Anhui Agricultural Sciences, 2018,46(32):26-32.(in Chinese with English abstract)
[22]	吕芃. 高粱资源材料茎秆含糖量等相关性状的关联定位分析[D]. 北京: 中国农业科学院, 2014.
	LÜ P. Association mapping analysis of sugar-related traits in Sorghum[Sorghum bicolor(L.)][D]. Beijing: Chinese Academy of Agricultural Sciences, 2014. (in Chinese with English abstract)
[23]	李伟忠. 玉米自交系遗传多样性与表型性状关联分析及分子ID构建[D]. 哈尔滨: 东北农业大学, 2011.
	LI W Z. Genetic diversity evaluation, association analysis of phenotypic traits and molecular ID construction in maize[D]. Harbin: Northeast Agricultural University, 2011. (in Chinese with English abstract)

编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources
1	上葶穗 Shangtingsui	山西 Shanxi	38	打锣锤 Daluochui	内蒙古 Inner Mongolia	75	关东青 Guandongqing	辽宁 Liaoning
2	牛腿高粱 Niutui sorghum	北京 Beijing	39	黄罗伞 Huangluosan	山西 Shanxi	76	护脖香 Huboxiang	内蒙古 Inner Mongolia
3	白鞑子帽 Baidazi	北京 Beijing	40	九头翁 Jiutouweng	辽宁 Liaoning	77	吉7384 Ji7384	吉林 Jilin
4	黑壳白 Heikebai	北京 Beijing	41	小棒棒黄 Xiaobangbanghuang	辽宁 Liaoning	78	红杆1号 Honggan NO.1	辽宁 Liaoning
5	矬粘高粱 Sticky sorghum	北京 Beijing	42	牛心黄 Niuxinhuang	辽宁 Liaoning	79	DK77	香港 Hongkong
6	油心壶 Youxinhu	河北 Hebei	43	红壳 Red shell	辽宁 Liaoning	80	日本高粱1 Japan sorghum 1	日本 Japan
7	小白粮 Xiaobailiang	河北 Hebei	44	蛇眼 Sheyan	辽宁 Liaoning	81	日本高粱3 Japan sorghum 3	日本 Japan
8	地丁 Diding	河北 Hebei	45	散穗大米粱 Scattered rice sorghum	辽宁 Liaoning	82	日本高粱4 Japan sorghum 4	日本 Japan
9	矮高粱 Dwarf sorghum	河北 Hebei	46	散穗红壳 Panicled red shell	辽宁 Liaoning	83	日本高粱6 Japan sorghum 6	日本 Japan
10	九叶红 Nine-leaves red	山西 Shanxi	47	大红袍 Dahongpao	山东 Shandong	84	KAMADRI KAMADRI	美国 America
11	大软茭 Daruanjiao	山西 Shanxi	48	柳子高粱 Liuzi sorghum	山东 Shandong	85	太仓甜高粱 Taicang sweet sorghum	江苏 Jiangsu
12	本地软高粱 Native soft sorghum	内蒙古 Inner Mongolia	49	黑壳二尾 Heikeerwei	山西 Shanxi	86	苏丹草 Sudangrass	苏丹 Sudan
13	旧高粱 Old sorghum	内蒙古 Inner Mongolia	50	扫帚糜子 Broom corn millet	辽宁 Liaoning	87	119 高粱 119 sorghum	辽宁 Liaoning
14	红高粱 Red sorghum	内蒙古 Inner Mongolia	51	三代高粱 Three generation sorghum	北京 Beijing	88	二户高粱 Erhu sorghum	辽宁 Liaoning
15	红壳高粱 Red-shell sorghum	山西 Shanxi	52	大黑帽 Daheimao	山西 Shanxi	89	二路黄壳 Erluhuangke	辽宁 Liaoning
16	红秆粘高粱 Red stalk sticky sorghum	山西 Shanxi	53	上井高粱 Shangjing sorghum	山西 Shanxi	90	小粘棒 Xiaozhanbang	辽宁 Liaoning
17	红秆高粱 Red stem sorghum	山西 Shanxi	54	牛尾巴高粱 Niuweiba sorghum	山西 Shanxi	91	平顶香 Pingdingxiang	黑龙江 Heilongjiang
18	软高粱 Soft sorghum	山西 Shanxi	55	打锣锤 Daluochui	山西 Shanxi	92	甜选41 Tianxuan NO.41	北京 Beijing
19	狼尾巴 Langweiba	山西 Shanxi	56	金光 Jinguang	山西 Shanxi	93	SUGAR drip	日本 Japan
20	狼尾巴软茭 Langweiba soft Jiao	山西 Shanxi	57	拐脖三 Guaibosan	山西 Shanxi	94	ALBAUGH	美国 America
21	紧穗高粱 Tight ear sorghum	山西 Shanxi	58	黄罗伞 Huangluosan	山西 Shanxi	95	BATHURST SUMAC	美国 America
22	睁眼三 Zhengyansan	山西 Shanxi	59	铁沙链 Tieshalian	山西 Shanxi	96	CLUBHEAD-3	美国 America
23	喜鹊白 Xiquebai	山西 Shanxi	60	10032	美国 America	97	COLLIER-2	美国 America
24	锣锤穗 Luochuisui	山西 Shanxi	61	DURA EL MEK	美国 America	98	COLLIER-7	美国 America
25	白糯高粱 White waxy sorghum	陕西 Shaanxi	62	金华当地种 Jinhuadangdizhong	浙江 Zhejiang	99	COLMAN (M)	美国 America
编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources
26	糯高粱 Waxy sorghum	四川 Sichuan	63	高河高粱 Gaohe sorghum	安徽 Anhui	100	EARLY FOLGER	美国 America
27	马尾高粱 Mawei sorghum	四川 Sichuan	64	ALBAUGH	美国 America	101	EARLY SUMAC	美国 America
28	矮高粱 Dwarf sorghum	四川 Sichuan	65	BATHURST SUMAC	美国 America	102	LEOTI-3	美国 America
29	板江高粱 Banjiang sorghum	广西 Guangxi	66	苏马克 Sumake	美国 America	103	LEOTI-4	美国 America
30	黑色在来种 Heiseizailaizhong	日本 Japan	67	Bjk236	北京 Beijing	104	MN-55	美国 America
31	MN-3080	美国 America	68	甜选42 Tianxuan NO.42	北京 Beijing	105	MN-3808	美国 America
32	MN-4244	美国 America	69	粘高粱 Sticky sorghum	河北 Hebei	106	MN-2609	美国 America
33	黑壳棒子 Black shell bonzi	辽宁 Liaoning	70	粘高粱 Sticky sorghum	辽宁 Liaoning	107	654	辽宁 Liaoning
34	北平五号 Beiping N0.5	北京 Beijing	71	LN8125	辽宁 Liaoning	108	BTX623	美国 America
35	南苑红二号 Nanyuanhong NO.2	北京 Beijing	72	LN8155	辽宁 Liaoning	109	LTR108	辽宁 Liaoning
36	八叶齐 Bayeqi	山西 Shanxi	73	Roma	美国 America	110	QG7	贵州 Guizhou
37	大关东 Daguandong	山西 Shanxi	74	253	辽宁 Liaoning

编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources
1	上葶穗 Shangtingsui	山西 Shanxi	38	打锣锤 Daluochui	内蒙古 Inner Mongolia	75	关东青 Guandongqing	辽宁 Liaoning
2	牛腿高粱 Niutui sorghum	北京 Beijing	39	黄罗伞 Huangluosan	山西 Shanxi	76	护脖香 Huboxiang	内蒙古 Inner Mongolia
3	白鞑子帽 Baidazi	北京 Beijing	40	九头翁 Jiutouweng	辽宁 Liaoning	77	吉7384 Ji7384	吉林 Jilin
4	黑壳白 Heikebai	北京 Beijing	41	小棒棒黄 Xiaobangbanghuang	辽宁 Liaoning	78	红杆1号 Honggan NO.1	辽宁 Liaoning
5	矬粘高粱 Sticky sorghum	北京 Beijing	42	牛心黄 Niuxinhuang	辽宁 Liaoning	79	DK77	香港 Hongkong
6	油心壶 Youxinhu	河北 Hebei	43	红壳 Red shell	辽宁 Liaoning	80	日本高粱1 Japan sorghum 1	日本 Japan
7	小白粮 Xiaobailiang	河北 Hebei	44	蛇眼 Sheyan	辽宁 Liaoning	81	日本高粱3 Japan sorghum 3	日本 Japan
8	地丁 Diding	河北 Hebei	45	散穗大米粱 Scattered rice sorghum	辽宁 Liaoning	82	日本高粱4 Japan sorghum 4	日本 Japan
9	矮高粱 Dwarf sorghum	河北 Hebei	46	散穗红壳 Panicled red shell	辽宁 Liaoning	83	日本高粱6 Japan sorghum 6	日本 Japan
10	九叶红 Nine-leaves red	山西 Shanxi	47	大红袍 Dahongpao	山东 Shandong	84	KAMADRI KAMADRI	美国 America
11	大软茭 Daruanjiao	山西 Shanxi	48	柳子高粱 Liuzi sorghum	山东 Shandong	85	太仓甜高粱 Taicang sweet sorghum	江苏 Jiangsu
12	本地软高粱 Native soft sorghum	内蒙古 Inner Mongolia	49	黑壳二尾 Heikeerwei	山西 Shanxi	86	苏丹草 Sudangrass	苏丹 Sudan
13	旧高粱 Old sorghum	内蒙古 Inner Mongolia	50	扫帚糜子 Broom corn millet	辽宁 Liaoning	87	119 高粱 119 sorghum	辽宁 Liaoning
14	红高粱 Red sorghum	内蒙古 Inner Mongolia	51	三代高粱 Three generation sorghum	北京 Beijing	88	二户高粱 Erhu sorghum	辽宁 Liaoning
15	红壳高粱 Red-shell sorghum	山西 Shanxi	52	大黑帽 Daheimao	山西 Shanxi	89	二路黄壳 Erluhuangke	辽宁 Liaoning
16	红秆粘高粱 Red stalk sticky sorghum	山西 Shanxi	53	上井高粱 Shangjing sorghum	山西 Shanxi	90	小粘棒 Xiaozhanbang	辽宁 Liaoning
17	红秆高粱 Red stem sorghum	山西 Shanxi	54	牛尾巴高粱 Niuweiba sorghum	山西 Shanxi	91	平顶香 Pingdingxiang	黑龙江 Heilongjiang
18	软高粱 Soft sorghum	山西 Shanxi	55	打锣锤 Daluochui	山西 Shanxi	92	甜选41 Tianxuan NO.41	北京 Beijing
19	狼尾巴 Langweiba	山西 Shanxi	56	金光 Jinguang	山西 Shanxi	93	SUGAR drip	日本 Japan
20	狼尾巴软茭 Langweiba soft Jiao	山西 Shanxi	57	拐脖三 Guaibosan	山西 Shanxi	94	ALBAUGH	美国 America
21	紧穗高粱 Tight ear sorghum	山西 Shanxi	58	黄罗伞 Huangluosan	山西 Shanxi	95	BATHURST SUMAC	美国 America
22	睁眼三 Zhengyansan	山西 Shanxi	59	铁沙链 Tieshalian	山西 Shanxi	96	CLUBHEAD-3	美国 America
23	喜鹊白 Xiquebai	山西 Shanxi	60	10032	美国 America	97	COLLIER-2	美国 America
24	锣锤穗 Luochuisui	山西 Shanxi	61	DURA EL MEK	美国 America	98	COLLIER-7	美国 America
25	白糯高粱 White waxy sorghum	陕西 Shaanxi	62	金华当地种 Jinhuadangdizhong	浙江 Zhejiang	99	COLMAN (M)	美国 America
编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources	编号 No.	名称 Name	来源 Sources
26	糯高粱 Waxy sorghum	四川 Sichuan	63	高河高粱 Gaohe sorghum	安徽 Anhui	100	EARLY FOLGER	美国 America
27	马尾高粱 Mawei sorghum	四川 Sichuan	64	ALBAUGH	美国 America	101	EARLY SUMAC	美国 America
28	矮高粱 Dwarf sorghum	四川 Sichuan	65	BATHURST SUMAC	美国 America	102	LEOTI-3	美国 America
29	板江高粱 Banjiang sorghum	广西 Guangxi	66	苏马克 Sumake	美国 America	103	LEOTI-4	美国 America
30	黑色在来种 Heiseizailaizhong	日本 Japan	67	Bjk236	北京 Beijing	104	MN-55	美国 America
31	MN-3080	美国 America	68	甜选42 Tianxuan NO.42	北京 Beijing	105	MN-3808	美国 America
32	MN-4244	美国 America	69	粘高粱 Sticky sorghum	河北 Hebei	106	MN-2609	美国 America
33	黑壳棒子 Black shell bonzi	辽宁 Liaoning	70	粘高粱 Sticky sorghum	辽宁 Liaoning	107	654	辽宁 Liaoning
34	北平五号 Beiping N0.5	北京 Beijing	71	LN8125	辽宁 Liaoning	108	BTX623	美国 America
35	南苑红二号 Nanyuanhong NO.2	北京 Beijing	72	LN8155	辽宁 Liaoning	109	LTR108	辽宁 Liaoning
36	八叶齐 Bayeqi	山西 Shanxi	73	Roma	美国 America	110	QG7	贵州 Guizhou
37	大关东 Daguandong	山西 Shanxi	74	253	辽宁 Liaoning

性状 Traits	中胚轴长 Mesocotyl length	胚芽鞘长 Coleoptile length	根长 Root length	百粒重 Hundred-grain weight
中胚轴长	1.000
Mesocotyl length
胚芽鞘长	0.251^**	1.000
Coleoptile length
根长	-0.234^*	0.385^**	1.000
Root length
百粒重	0.022	0.602^**	0.560^**	1.000
Hundred- grain weight

性状 Traits	中胚轴长 Mesocotyl length	胚芽鞘长 Coleoptile length	根长 Root length	百粒重 Hundred-grain weight
中胚轴长	1.000
Mesocotyl length
胚芽鞘长	0.251^**	1.000
Coleoptile length
根长	-0.234^*	0.385^**	1.000
Root length
百粒重	0.022	0.602^**	0.560^**	1.000
Hundred- grain weight

标记 Markers	等位基因数/个 Allele number	有效等位基因数/个 Effective allele number	Shannon’s信息指数 Shannon’s information index
sm01005	4	2.93	1.189 1
sm01063	5	2.84	1.171 6
sm01102	8	2.04	1.006 5
sm01113	6	2.01	1.010 5
sm01140	8	3.32	1.451 0
sm02004	14	5.35	1.989 0
sm02018	5	2.66	1.106 3
sm02024	4	1.60	0.696 8
sm02041	4	1.75	0.792 2
sm02053	6	1.36	0.603 8
sm02073	7	2.29	1.167 7
sm03005	6	1.26	0.503 5
sm03023	7	1.97	0.954 6
sm03084	5	2.29	0.952 4
sm03101	5	1.96	0.860 3
sm03109	8	4.27	1.616 2
sm04001	4	1.82	0.754 3
sm04010	7	2.82	1.382 3
sm04028	2	1.33	0.414 7
标记 Markers	等位基因数/个 Allele number	有效等位基因数/个 Effective allele number	Shannon’s信息指数 Shannon’s information index
sm04082	6	2.86	1.262 5
sm04112	6	2.47	1.236 4
sm05007	8	4.35	1.681 0
sm05018	10	5.13	1.820 8
sm05033	5	1.94	0.960 1
sm05080	13	4.92	1.926 4
sm05088	8	2.04	1.121 5
sm05097	6	3.98	1.535 9
sm06003	7	4.26	1.579 5
sm06024	3	1.35	0.505 1
sm06048	5	3.22	1.273 8
sm06072	5	1.21	0.421 0
sm06084	4	2.53	1.056 0
sm06130	4	1.21	0.380 1
sm07003	9	4.34	1.670 0
sm07038	8	1.81	0.937 4
sm07063	7	2.02	1.050 6
sm07087	8	3.68	1.472 9
sm07093	4	2.22	0.890 9
sm08001	5	2.98	1.218 2
sm08025	5	2.53	1.121 9
sm08055	7	1.95	1.025 5
sm08075	3	1.58	0.649 7
sm08092	7	2.83	1.285 5
sm09004	4	1.87	0.753 0
sm09023	9	3.97	1.600 4
sm09061	5	1.43	0.635 4
sm09083	7	3.31	1.370 1
sm09094	5	1.72	0.780 2
sm09102	6	2.78	1.319 5
sm10006	6	3.66	1.452 1
sm10025	6	3.73	1.472 8
sm10039	6	1.92	1.032 1
sm10097	8	3.59	1.493 8
sm10081	9	3.01	1.401 4
总计 Total	339	144.26	—
平均Mean	6.28	2.67	1.129 9

Association analysis between SSR markers and germination-related traits in sorghum germplasm resources

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性状 Traits	标记 Markers	染色体 Chromosome	位置 Position	GLM		MLM
性状 Traits	标记 Markers	染色体 Chromosome	位置 Position	P值 P value	贡献率 R²/%	P值 P value	贡献率 R²/%
中胚轴长	sm04028	4	17481728	7.49×10^-11	28.05
Mesocotyl length	sm08075	8	48823349	4.85×10^-9	25.04
	sm09061	9	39002689	1.54×10^-8	25.45
	sm05088	5	53328744	5.26×10^-8	22.52	8.16×10^-5	18.55
	sm02041	2	47494750	5.29×10^-7	19.70
	sm03023	3	17912117	6.91×10^-6	16.24
	sm06130	6	52982164	2.35×10^-5	13.66
	sm03101	3	68989564	1.64×10^-4	11.49
	sm07063	7	37676193	5.30×10^-4	11.18
胚芽鞘长	sm09094	9	55329987	2.31×10^-6	18.12
Coleoptile length	sm01102	1	20521949	4.91×10^-5	11.99
	sm01113	1	45193828	1.05×10^-4	12.65
	sm10039	10	22467847	3.77×10^-4	11.22
	sm01140	1	66365336	5.13×10^-4	11.52
根长
Root length	sm10097	10	46436392	9.35×10^-7	28.03
百粒重
Hundred-grain weight	sm09094	9	55329987	2.85×10^-5	17.11
	sm01113	1	45193828	4.58×10^-4	12.15

性状 Traits	标记-等位变异 Marker-Allele	染色体 Chromosome	位置 Position	载体材料数量 Number of carrier materials	表型效应 Phenotypic effects	载体材料 Carrier materials
中胚轴长 Mesocotyl length	sm02041-1	2	47494750	80	6.67	BATHURST SUMAC,EARLY FOLGER,253,平顶香,BATHURST SUMAC BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang, BATHURST SUMAC
	sm03023-1	3	17912117	73	5.53	CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,平顶香 CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang
	sm03101-1	3	68989564	72	4.17	CLUBHEAD-3,BATHURST SUMAC,253,平顶香,COLLIER-7 CLUBHEAD-3,BATHURST SUMAC,253,Pingdingxiang,COLLIER-7
	sm04028-1	4	17481728	94	8.83	CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,平顶香 CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang
	sm05088-1	5	53328744	75	8.34	CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,平顶香 CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang
	sm06130-1	6	52982164	99	7.94	CLUBHEAD-3,EARLY FOLGER,253,平顶香,BATHURST SUMAC CLUBHEAD-3,EARLY FOLGER,253,Pingdingxiang,BATHURST SUMAC
	sm07063-1	7	37676193	73	5.49	BATHURST SUMAC,EARLY FOLGER,253,平顶香,BATHURST SUMAC BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang,BATHURST SUMAC
	sm08075-1	8	48823349	85	7.60	CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,平顶香 CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang
	sm09061-1	9	39002689	88	9.12	CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,平顶香 CLUBHEAD-3,BATHURST SUMAC,EARLY FOLGER,253,Pingdingxiang
胚芽鞘长 Coleoptile length	sm01102-1	1	20521949	72	0.76	狼尾巴软茭,扫帚糜子,散穗大米粱,散穗红壳,旧高粱 Langweiba soft Jiao,Broom corn millet,Scattered rice sorghum,Panicled red shell,Old sorghum
	sm01113-1	1	45193828	73	0.78	狼尾巴软茭,扫帚糜子,散穗大米粱,散穗红壳,旧高粱 Langweiba soft Jiao,Broom corn millet,Scattered rice sorghum,Panicled red shell,Old sorghum
	sm01140-1	1	66365336	52	0.64	狼尾巴软茭,扫帚糜子,本地软高粱,小棒棒黄,蛇眼 Langweiba soft Jiao,Broom corn millet,Native soft sorghum,Xiaobangbanghuang,Sheyan
	sm09094-1	9	55329987	65	0.72	狼尾巴软茭,扫帚糜子,散穗大米粱,散穗红壳,旧高粱 Langweiba soft Jiao,Broom corn millet,Scattered rice sorghum,Panicled red shell,Old sorghum
	sm10039-1	10	22467847	77	0.72	狼尾巴软茭,扫帚糜子,散穗大米粱,散穗红壳,旧高粱 Langweiba soft Jiao,Broom corn millet,Scattered rice sorghum,Panicled red shell,Old sorghum
根长 Root length	sm10097-1	10	46436392	40	3.21	八叶齐,北平五号,油心壶,拐脖三,小白粮 Bayeqi,Beipin NO.5,Youxinhu,Guaibosan(wuxiang),Xiaobailiang
百粒重 Hundred- grain weight	sm09094-1	9	55329987	65	0.89	上井高粱,打锣锤,散穗大米粱,八叶齐,打锣锤 Shangjing sorghum,Daluochui,Scattered rice sorghum,Bayeqi,Daluochui(anze)
	sm01113-1	1	45193828	73	0.98	上井高粱,三代高粱,打锣锤,日本高粱1,散穗大米粱 Shangjing sorghum,Three generation sorghum,Daluochui,Japan sorghum 1,Scattered rice sorghum

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