Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry

doi:10.3969/j.issn.1004-1524.2022.07.09

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (7): 1423-1430.DOI: 10.3969/j.issn.1004-1524.2022.07.09

• Horticultural Science • Previous Articles Next Articles

Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry

YANG Xiaofang¹(), LI Yunduan², SUN Yunfan², LI Shaojia², MIAO Lixiang¹, ZHANG Yuchao¹, JIANG Guihua¹^,^*()

1. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2021-09-16 Online:2022-07-25 Published:2022-07-26
Contact: JIANG Guihua

Abstract

Abstract:

In order to study the difference of strawberry fruit quality formation under substrate cultivation and soil cultivation, and explore its influence law, the contents of sucrose and citric acid and the expression of related genes in different development stages of strawberry were measured. The results showed that contents of sucrose and citric acid in soil culture were higher than those in substrate culture. The difference of sucrose accumulation was mainly reflected in the tip of strawberry fruit, and the difference of citric acid accumulation was in the tip and base of strawberry fruit. FaSPS2 gene was the key gene for the difference of sucrose accumulation between soil culture and substrate culture, which was positively correlated with sucrose accumulation. The expression of FaACO1 and FaACO3 genes were negatively correlated with the accumulation of citric acid. The expression of FaACO1 and FaACO3 in matrix culture were significantly higher than those in soil culture, resulting in the citric acid content of mature strawberry fruit in matrix culture was lower than that in soil culture. Therefore, FaSPS2, FaACO1 and FaACO3 genes played important role in the accumulation of sucrose and citric acid content of Yuexin strawberry. Soil cultivation might be more conducive to the accumulation of sucrose and citric acid content of Yuexin strawberry than substrate cultivation.

Key words: substrate cultivation, soil cultivation, strawberry, sucrose, citric acid

CLC Number:

S668.4

YANG Xiaofang, LI Yunduan, SUN Yunfan, LI Shaojia, MIAO Lixiang, ZHANG Yuchao, JIANG Guihua. Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry[J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1423-1430.

Figures/Tables 7

Table 1 Primers for real-time quantitative PCR

基因 Gene	编码蛋白 Encoding protein	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')
FaSUS1	SUS	CCCTGATTCTGACCTTTACTGG	GATGATGAAGTCGGTGTGGTT
FaSUS2	SUS	AAGAAGAACTTGTAGATGGGAGGTC	ATAATTGAAGAAAGGTGGCGGT
FaSPS1	SPS	GAGCCTTGAATGTCCCAATGT	ATCTCCTGTCTGGTGCTGGTT
FaSPS2	SPS	ACTGTGCAAAGTTGGCGTCTT	GATCATCGCAATCCACGGCTA
FaSPS3	SPS	CGTAGATTGGAGTTATGGAGAGC	CGAATGATGTAAGAACCACTGC
FaCS1	CS	AATCTCTGCGGCGGTTTGAC	TGGCTTTGATGGTTCCTTGTTC
FaCS2	CS	CATCCCACTGCCGATGTCTT	GACTCTGATGGTTGGCTGCT
FaCS4 FaACO1 FaACO3	CS ACO ACO	AATGAGAGGAATGACGGGGT CCCCAAAACAATCCATCATCC TTGGTTGGTATGGGTATTATTCC	TAATCTGGAACTTTGGCACGA TTCCTCACCAGTCAATCCAAGA GCTTTATCTCACTGATGTTGCTAGG
FaRIB413	RIB	ACCGTTGATTCGCACAATTGGTCATCG	TACTGCGGGTCGGCAATCGGACG

Fig.1 Sucrose content of strawberry cultivated under substrate and soil during different fruit developmental periods A, Tip; B, Base. W, White stage; T, Turning stage; IR, Intermediate red stage; R, Full red stage. The same as below.

Fig.2 Citric acid content of strawberry cultivated under substrate and soil during different fruit developmental periods A, Tip; B, Base.

Fig.3 Expression pattern of genes related to sucrose anabolism under substrate and soil cultivation

Fig.4 Correlation analysis between sucrose content and relative expression of FaSPS2

Fig.5 Expression patterns of genes related to citric acid degradation under substrate culture and soil culture

Fig.6 Correlation analysis between citric acid content and relative expression of FaACO1 and FaACO3

References 20

[1]	LI M J, FENG F J, CHENG L L. Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development[J]. PLoS One, 2012, 7(3): e33055.
[2]	TANASE K, YAMAKI S. Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit[J]. Plant and Cell Physiology, 2000, 41(4): 408-414. DOI URL
[3]	ZHANG N, JIANG J, YANG Y L, et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. Journal of Zhejiang University-Science B (Biomedicine & Biotechnology), 2015, 16(10): 845-856. DOI URL
[4]	DENG W, LUO K M, LI Z G, et al. Overexpression of Citrus junos mitochondrial citrate synthase gene in Nicotiana benthamiana confers aluminum tolerance[J]. Planta, 2009, 230(2): 355-365. DOI URL
[5]	MORGAN M J, OSORIO S, GEHL B, et al. Metabolic engineering of tomato fruit organic acid content guided by biochemical analysis of an introgression line[J]. Plant Physiology, 2012, 161(1): 397-407. DOI URL
[6]	张豫超, 杨肖芳, 苗立祥, 等. 优质抗病草莓新品种: ‘越心’的选育[J]. 果树学报, 2015, 32(6): 1294-1296, 996.
	ZHANG Y C, YANG X F, MIAO L X, et al. ‘Yuexin’, a new strawberry cultivar with high quality and disease resistance[J]. Journal of Fruit Science, 2015, 32(6): 1294-1296, 996. (in Chinese with English abstract)
[7]	ZHANG Y Y, YIN X R, XIAO Y W, et al. An ethylene response factor-MYB transcription complex regulates furaneol biosynthesis by activating quinone oxidoreductase expression in strawberry[J]. Plant Physiology, 2018, 178(1): 189-201. DOI URL
[8]	LI S J, YIN X R, WANG W L, et al. Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3[J]. Journal of Experimental Botany, 2017, 68(13): 3419-3426. DOI URL
[9]	CHANG S J, PURYEAR J, CAIRNEY J. A simple and efficient method for isolating RNA from pine trees[J]. Plant Molecular Biology Reporter, 1993, 11(2): 113-116. DOI URL
[10]	JUNG S, LEE T, CHENG C H, et al. 15 years of GDR: new data and functionality in the Genome Database for Rosaceae[J]. Nucleic Acids Research, 2019, 47(D1): D1137-D1145.
[11]	AMIL-RUIZ F, GARRIDO-GALA J, BLANCO-PORTALES R, et al. Identification and validation of reference genes for transcript normalization in strawberry (Fragaria×ananassa) defense responses[J]. PLoS One, 2013, 8(8): e70603.
[12]	施羊林, 陈小文, 许庆广, 等. 不同栽培介质及EC值对温室草莓生长的影响[J]. 山西农业大学学报(自然科学版), 2016, 36(8): 562-566.
	SHI Y L, CHEN X W, XU Q G, et al. Effects of different growing media and EC for greenhouse grown strawberries[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2016, 36(8): 562-566. (in Chinese with English abstract)
[13]	向发云, 曾祥国, 张鹏, 等. “晶瑶”草莓基质栽培与有土栽培生产性状比较[J]. 湖北农业科学, 2011, 50(23): 4858-4860, 4899.
	XIANG F Y, ZENG X G, ZHANG P, et al. Comparison on substrate culture and soil culture of ‘Jingyao’ strawberry in plastic house[J]. Hubei Agricultural Sciences, 2011, 50(23): 4858-4860, 4899. (in Chinese with English abstract)
[14]	谢鸣, 张上隆, 陈俊伟, 等. 基质培对草莓糖酸和某些生理特性的影响[J]. 果树学报, 2004, 21(6): 560-564.
	XIE M, ZHANG S L, CHEN J W, et al. Effect of substrate culture on sugar and acid content and some physiological characteristics of strawberry fruit[J]. Journal of Fruit Science, 2004, 21(6): 560-564. (in Chinese with English abstract)
[15]	邓胜艺, 张佳芮, 姚磊. 基质栽培与土壤栽培罗马花椰菜品质产量的比较研究[J]. 园艺与种苗, 2020, 40(6): 13-14.
	DENG S Y, ZHANG J R, YAO L. Comparative study on quality and production of Roman broccoli cultivated with substrate and soil[J]. Horticulture & Seed, 2020, 40(6): 13-14. (in Chinese with English abstract)
[16]	魏韩英. 海岛草莓基质栽培试验[J]. 农业与技术, 2017, 37(8): 48-49.
	WEI H Y. Culture experiment of island strawberry with substrate[J]. Agriculture and Technology, 2017, 37(8): 48-49. (in Chinese)
[17]	刘中良, 高昕, 张艳艳, 等. 基质栽培与土壤栽培番茄品质产量的比较研究[J]. 江苏农业科学, 2020, 48(1): 124-127.
	LIU Z L, GAO X, ZHANG Y Y, et al. Comparative study on quality and yield of tomato under substrate cultivation and soil cultivation[J]. Jiangsu Agricultural Sciences, 2020, 48(1): 124-127. (in Chinese)
[18]	MIAO L X, ZHANG Y C, YANG X F, et al. Fruit quality, antioxidant capacity, related genes, and enzyme activities in strawberry (Fragaria×ananassa) grown under colored plastic films[J]. HortScience, 2017, 52(9): 1241-1250. DOI URL
[19]	RUAN Y L, JIN Y, YANG Y J, et al. Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat[J]. Molecular Plant, 2010, 3(6): 942-955. DOI URL
[20]	IANNETTA P P M, ESCOBAR N M, ROSS H A, et al. Identification, cloning and expression analysis of strawberry (Fragaria×ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenase[J]. Physiologia Plantarum, 2004, 121(1): 15-26. DOI URL

Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LYU Shufang, ZHANG Hongxiao, XU Huawei, ZHAO Xingli. Analysis of GhSuSy expression by particle bombardment during initiation of cotton fibers [J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1361-1368.
[2]	LI Qingbin, QIN Benben, LI Yingying, FAN Kaifeng, YANG Dong, CHEN Lei, LIU Kun. Effects of continuous rain and sunless weather on microclimate, strawberry growth and quality in greenhouse [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 831-839.
[3]	ZHANG Weimei, ZHANG Guwen, FENG Zhijuan, LIU Na, WANG Bin, BU Yuanpeng. Research progress on genetic and regulatory mechanisms of sucrose in vegetable soybean seeds [J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2446-2456.
[4]	FENG Jiangpeng, QIU Liping, LIANG Xiuyan, CHEN Bixiu, XIA Haiyang, PENG Chunlong, ZHONG Yongjun. Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity [J]. , 2020, 32(5): 831-839.
[5]	QIU Jieren, CHAI Weiguo, TONG Jianxin, ZHOU Liping, WANG Shuzhen. Optimization of strawberry gynoecium protein extraction method for mass spectrum-based proteomic study [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2186-2191.
[6]	WANG Shuzhen, ZHOU Liping, QIU Jieren, TONG Jianxin, YU Hong, CHAI Weiguo, LAI Wenguo. Comparative study on traits of progeny and parents of strawberry interspecific cross [J]. , 2020, 32(10): 1780-1787.
[7]	WANG Shuzhen, ZHANG Yahui, QIU Jieren, ZHOU Liping, CHEN Sisi, CHAI Weiguo, MAO Bizeng. Comparison of characteristics of strawberry varieties with different ploidy [J]. , 2019, 31(6): 893-899.
[8]	MA Yisheng, HONG Chunlai, WANG Weiping, YAO Yanlai, ZHU Fengxiang, CHEN Xiaoyang, XUE Zhiyong. Effects of substrates prepared from Dendrobium officinale cultivation waste and mushroom residue on growth, yield and quality of strawberry [J]. , 2018, 30(7): 1175-1181.
[9]	ZHANG Qing. Effects of storage temperature on fruit quality of strawberry varieties Yuexin and Akihime [J]. , 2018, 30(4): 600-606.
[10]	ZHAO Guofu, WANG Jin'ao, XIE Tinghui. Changes of nutritional quality and aroma during fruit development of Fragaria ananassa Duch. cv Benihoppe [J]. , 2018, 30(12): 2044-2048.
[11]	LIU Ziying, HUANG Lei, YUAN Bin, LIU Xiaolin, XU Shengguang, HUANG Tao. Study on screening degrading bacteria and its degradation effect on benzoic acid of autotoxic compounds in strawberry cropping obstacle [J]. , 2018, 30(10): 1699-1704.
[12]	CANG Tao, SONG Wen, ZHAO Xueping, WU Changxing, WU Shenggan, CHEN Liping, XU Mingfei, ZHAO Huiyu, WANG Qiang. Effects of forchlorfenuron on strawberry hollow and malformation [J]. , 2017, 29(9): 1537-1543.
[13]	CHEN Ying, XIAO Chunlin, LUO Yanna, ZHANG Xiying, LIU Jiangna. Detoxification protocal optimization and virus detection in strawberry variety Hongyan [J]. , 2017, 29(6): 966-970.
[14]	FENG Chen, TANG Haoru, JIANG Leiyu, SONG Xia, ZHANG Yunting, YE Yuntian, CHEN Qing, SUN Bo. Analysis of codon usage bias of specific genes in strawberry transcriptome under the red and blue light [J]. , 2017, 29(4): 566-574.
[15]	WU Shenggan, GUAN Wenbi, LIU Xinju, AN Xuehua, LYU Lu, ZHAO Xueping. Comparison on maximum residue limits standards of pesticides in strawberry [J]. , 2017, 29(3): 460-468.