Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities

doi:10.3969/j.issn.1004-1524.2021.12.22

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2415-2422.DOI: 10.3969/j.issn.1004-1524.2021.12.22

• Food Science • Previous Articles Next Articles

Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities

MA Zijia¹(), ZHANG Kaijie², LU Shengmin¹^,³^,^*(), XU Zhihao⁴, ZHENG Meiyu³, LIU Zhe³, WANG Lu³, YANG Ying³

1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China
2. Jiangsu Kaiyi Intelligent Technology Co., Ltd., Wuxi 214100, China
3. Institute of Food Science/Key Laboratory of Fruit and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
4. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-05-13 Online:2021-12-25 Published:2022-01-10
Contact: LU Shengmin

Abstract

Abstract:

Nineteen varieties of celeries, including Green stalk celery, White celery, Purple celery, etc. were taken as research materials. Water extracts of the edible parts (stems and leaves) of the celeries investigated were prepared under the same conditions(1∶30 g·mL^-1in solid-liquid ratio, 2 h in extraction time and 60 ℃ in extraction temperature) to screen out the varieties and their edible parts with higher in vitro angiotensin converting enzyme (ACE) inhibitory activity, total phenols and total flavonoids contents. The results indicated that in vitro ACE inhibitory activities of celery leaves extracted with water were significantly higher than those with ethanol solution (P<0.05). The in vitro ACE inhibitory activities in leaf aqueous extracts of different celery varieties concentrated in 80%-100%; total flavonoids contents were focused on 200-250 mg·L ^-1, with highest in White-stalk celery leaf and lowest in White celery leaf; total polyphenols contents were focused on 130-150 mg·L^-1, with highest in Purple celery leaf and lowest in Hollow stalk green celery. Correlation analysis indicated that in vitro ACE inhibitory activity was positively related with both of total flavonoids content and total polyphenols content, with their correlation coefficients being 0.3-0.6. Green stalk celery leaf had the lowest half inhibitory concentration (IC₅₀) values of ACE (1.258 mg·mL ^-1), followed by Four season celery (1.368 mg·mL^-1) and White celery leaf (1.829 mg·mL^-1).

Key words: celery, varieties, angiotensin converting enzyme inhibitory activity, total flavonoids, total phenolics

CLC Number:

S636.3

MA Zijia, ZHANG Kaijie, LU Shengmin, XU Zhihao, ZHENG Meiyu, LIU Zhe, WANG Lu, YANG Ying. Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2415-2422.

Figures/Tables 5

References 24

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编号 No.	品种名称 Celery varieties	体外ACE抑制活性 ACE inhibitory activity/%	总黄酮含量 Total flavonoids content/(mg·L^-1)	总酚含量 Total phenolics content/(mg·L^-1)
1	四季慢芹菜Four seasoncelery	92.80±1.80 ef	224.23±1.35 g	170.45±1.46 b
2	紫芹Purple celery	105.13±1.41 a	239.55±3.96 d	182.49±3.65 a
3	白芹White celery	88.23±1.58 h	168.38±0.88 m	131.24±1.55 i
4	实杆芹Solid stalk celery	95.08±0.80 de	208.56±2.26 i	139.50±1.71 fgh
5	新云芹菜Xinyun celery	89.48±3.18 gh	181.89±1.32 l	128.56±0.86 i
6	白药芹Whiteherb celery	91.98±1.27 fg	242.25±5.12 d	139.50±1.55 fgh
7	铁杆青Tieganqing	89.92±1.15 gh	198.11±0.88 j	140.70±0.99 fg
8	泾川芹菜Jingchuan celery	79.79±1.02 i	253.24±3.97 b	143.08±2.18 f
9	空杆绿芹Hollow stalk greencelery	98.22±1.05 c	188.20±0.51 k	119.40±1.29 j
10	蒲芹Pu celery	94.27±1.00 ef	237.93±1.55 de	138.91±1.01 gh
11	白梗芹菜Whitestalk celery	98.24±0.98 bc	261.71±2.43 a	151.94±2.81 d
12	青梗芹菜Green stalk celery	103.86±1.62 a	261.35±0.44 a	160.80±0.61 c
13	重庆白芹菜Chongqing white celery	94.08±0.35 ef	235.05±4.44 ef	140.30±1.76 fg
14	凤凰芹菜Phoenix celery	96.78±1.04 cd	199.19±0.88 j	135.92±1.01 h
15	细叶芹菜Fine leafcelery	104.11±2.17 a	247.66±1.42 c	140.20±2.74 fg
16	青药芹Green herb celery	101.16±3.28 b	251.08±0.44 bc	141.79±0.84 fg
17	多伦实干芹菜Duolunsolid celery	97.95±1.36 cd	231.80±3.43 f	153.13±4.10 d
18	Ts123.010	96.81±0.46 cd	247.30±2.34 c	161.69±1.20 c
19	大芹菜Bigcelery	93.42±0.62 ef	218.11±1.92 h	147.26±2.20 e

编号 No.	品种名称 Celery varieties	体外ACE抑制活性 ACE inhibitory activity/%	总黄酮含量 Total flavonoids content/(mg·L^-1)	总酚含量 Total phenolics content/(mg·L^-1)
1	四季慢芹菜Four seasoncelery	92.80±1.80 ef	224.23±1.35 g	170.45±1.46 b
2	紫芹Purple celery	105.13±1.41 a	239.55±3.96 d	182.49±3.65 a
3	白芹White celery	88.23±1.58 h	168.38±0.88 m	131.24±1.55 i
4	实杆芹Solid stalk celery	95.08±0.80 de	208.56±2.26 i	139.50±1.71 fgh
5	新云芹菜Xinyun celery	89.48±3.18 gh	181.89±1.32 l	128.56±0.86 i
6	白药芹Whiteherb celery	91.98±1.27 fg	242.25±5.12 d	139.50±1.55 fgh
7	铁杆青Tieganqing	89.92±1.15 gh	198.11±0.88 j	140.70±0.99 fg
8	泾川芹菜Jingchuan celery	79.79±1.02 i	253.24±3.97 b	143.08±2.18 f
9	空杆绿芹Hollow stalk greencelery	98.22±1.05 c	188.20±0.51 k	119.40±1.29 j
10	蒲芹Pu celery	94.27±1.00 ef	237.93±1.55 de	138.91±1.01 gh
11	白梗芹菜Whitestalk celery	98.24±0.98 bc	261.71±2.43 a	151.94±2.81 d
12	青梗芹菜Green stalk celery	103.86±1.62 a	261.35±0.44 a	160.80±0.61 c
13	重庆白芹菜Chongqing white celery	94.08±0.35 ef	235.05±4.44 ef	140.30±1.76 fg
14	凤凰芹菜Phoenix celery	96.78±1.04 cd	199.19±0.88 j	135.92±1.01 h
15	细叶芹菜Fine leafcelery	104.11±2.17 a	247.66±1.42 c	140.20±2.74 fg
16	青药芹Green herb celery	101.16±3.28 b	251.08±0.44 bc	141.79±0.84 fg
17	多伦实干芹菜Duolunsolid celery	97.95±1.36 cd	231.80±3.43 f	153.13±4.10 d
18	Ts123.010	96.81±0.46 cd	247.30±2.34 c	161.69±1.20 c
19	大芹菜Bigcelery	93.42±0.62 ef	218.11±1.92 h	147.26±2.20 e

指标 Index	体外ACE抑制活性 In vitro ACE inhibitory activity	总黄酮含量 Total flavonoids content	总酚含量 Total phenolics content
体外ACE抑制活性 In vitro ACE inhibitory activity	1
总黄酮含量Total flavonoids content	0.363	1
总酚含量Total phenolics content	0.378	0.550	1

指标 Index	体外ACE抑制活性 In vitro ACE inhibitory activity	总黄酮含量 Total flavonoids content	总酚含量 Total phenolics content
体外ACE抑制活性 In vitro ACE inhibitory activity	1
总黄酮含量Total flavonoids content	0.363	1
总酚含量Total phenolics content	0.378	0.550	1

Antihypertensive active components in edible parts of different varieties of celeries and their in vitro angiotensin converting enzyme inhibitory activities

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