Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region

doi:10.3969/j.issn.1004-1524.2021.12.15

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2348-2357.DOI: 10.3969/j.issn.1004-1524.2021.12.15

• Environmental Science • Previous Articles Next Articles

Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region

SUI Xiran¹(), WANG Yan¹^,²^,^*(), LIU Yungen¹^,², ZHANG Yajie¹, WU Lifang¹

1. College of Ecology and Environment, Southwest Forestry University, Kunming 650224, China
2. Yunnan Key Laboratory of Ecological Environment Evolution and Pollution Control in Mountainous Rural Areas, Kunming 650224, China

Received:2020-09-09 Online:2021-12-25 Published:2022-01-10
Contact: WANG Yan

Abstract

Abstract:

In the present study, typical Pinus yunnanensis forest at rocky desertification region in southwest China was selected as the study area, analysis of variance (ANOVA), redundancy analysis (RDA) and high-throughput sequencing methods were applied to explore the response of soil nutrients and bacterial community composition and diversity to different altitudes, which would be helpful for the rocky desertification control and ecosystem vegetation restoration in this area. It was shown that the contents of soil total carbon, total nitrogen and total phosphorus increased fist and decreased then with increasing altitude. The highest content of soil total potassium was at altitude of 1 900 m, yet the lowest was at 1 600 m.The highest content of soil available nitrogen and available phosphorus was both at altitude of 1 600 m, while the lowest was at 1 300 m and 1 900 m, respectively. The highest content of available potassium was at altitude of 1 900 m, yet the lowest was at 1 600 m.The indexes of Chao1, Shannon increased first and then decreased with the elevated altitude, while the Simpson index showed the opposite trend. The dominant phyla of microbial community were Proteus, Acidobacteria, Actinomycetes and Curvularia, which accounted for 33.37%, 24.40%, 19.82%, 12.06%, respectively.The relative abundance of Proteus and Acidobacteria increased first and then decreased with the elevated altitude, while Curvularia showed the opposite trend. The highest relative abundance of Actinomycetes was found at altitude of 1 600 m, yet there was no significant difference within other altitudes. At class level, the dominant microbial community were Alphaproteobacteria, Acidobacteria, Gammaproteobacteria, Deltaproteobacteria, which accounted for 18.46%, 12.72%, 7.88%, 5.13%, respectively. The relative abundance of Alphaproteobacteria and Gammaproteobacteria increased first and then decreased, while Acidobacteria exhibited the opposite.There was no significant difference in the relative abundance of Deltaproteobacteria within different altitudes. Soil pH, and contents of total carbon, total nitrogen, total phosphorus, available nitrogen and available phosphorus were significantly (P<0.05) correlated with the bacterial diversity, and were main driving factors of bacterial community composition.

Key words: altitude, soil nutrients, high-throughput sequencing, bacteria

CLC Number:

S154.4

SUI Xiran, WANG Yan, LIU Yungen, ZHANG Yajie, WU Lifang. Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2348-2357.

Figures/Tables 6

References 38

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指标Index	1 300 m	1 600 m	1 900 m	2 100 m
经纬度	103°0'36″E,23°37'5″N	103°3'28″E,23°35'5″N	102°59'35″E,23°32'59″N	102°56'19″E,23°29'12″N
Latitude and longitude
坡向Aspect	阳坡Sunny slope	阳坡Sunny slope	阳坡Sunny slope	阳坡Sunny slope
坡位Slope position	中坡Middle slope	中坡Middle slope	中坡Middle slope	中坡Middle slope
坡度Slope/(°)	15	20	20	18
岩性Lithology	石灰岩Limestone	石灰岩Limestone	石灰岩Limestone	石灰岩Limestone
土壤类型 Soil type	红色石灰土 Red lime soil	红色石灰土 Red lime soil	黑色石灰土 Black lime soil	黑色石灰土 Black lime soil
基岩裸露率	50~70	50~70	30~50	30~50
Bedrock exposure rate/%
主要植物 Main plants	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、鬼针草Bidens pilosa L.	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、车桑子Dodonaea viscosa(L.) Jacq.	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、车桑子Dodonaea viscosa (L.) Jacq.、鬼针草Bidens pilosa L.、苦刺Sophora davidii	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、鬼针草Bidens pilosa L.
郁闭度Canopy density/%	15	20	20	20

指标Index	1 300 m	1 600 m	1 900 m	2 100 m
经纬度	103°0'36″E,23°37'5″N	103°3'28″E,23°35'5″N	102°59'35″E,23°32'59″N	102°56'19″E,23°29'12″N
Latitude and longitude
坡向Aspect	阳坡Sunny slope	阳坡Sunny slope	阳坡Sunny slope	阳坡Sunny slope
坡位Slope position	中坡Middle slope	中坡Middle slope	中坡Middle slope	中坡Middle slope
坡度Slope/(°)	15	20	20	18
岩性Lithology	石灰岩Limestone	石灰岩Limestone	石灰岩Limestone	石灰岩Limestone
土壤类型 Soil type	红色石灰土 Red lime soil	红色石灰土 Red lime soil	黑色石灰土 Black lime soil	黑色石灰土 Black lime soil
基岩裸露率	50~70	50~70	30~50	30~50
Bedrock exposure rate/%
主要植物 Main plants	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、鬼针草Bidens pilosa L.	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、车桑子Dodonaea viscosa(L.) Jacq.	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、车桑子Dodonaea viscosa (L.) Jacq.、鬼针草Bidens pilosa L.、苦刺Sophora davidii	云南松Pinus yunnanensis、紫茎泽兰Eupatorium adenophora Spreng.、鬼针草Bidens pilosa L.
郁闭度Canopy density/%	15	20	20	20

海拔 Altitude/m	序列数 Reads quantity	OTU数 OTU quantity	覆盖率 Coverage	Chao1指数 Chao1 index	Shannon指数 Shannon index	Simpson指数 Simpson index
1 300	38 795	1 851	0.989 2	2 178	5.98	0.007 3
1 600	29 951	2 256	0.981 1	2 739	6.44	0.004 6
1 900	40 857	2 595	0.986 5	3 019	6.71	0.002 8
2 100	51 272	2 197	0.990 1	2 592	5.95	0.008 6

海拔 Altitude/m	序列数 Reads quantity	OTU数 OTU quantity	覆盖率 Coverage	Chao1指数 Chao1 index	Shannon指数 Shannon index	Simpson指数 Simpson index
1 300	38 795	1 851	0.989 2	2 178	5.98	0.007 3
1 600	29 951	2 256	0.981 1	2 739	6.44	0.004 6
1 900	40 857	2 595	0.986 5	3 019	6.71	0.002 8
2 100	51 272	2 197	0.990 1	2 592	5.95	0.008 6

指标Index	pH	TC	TN	TP	TK	AN	AP	AK
Chao1指数Chao1 index	-0.864^**	0.985^**	0.947^**	0.940^**	0.262	0.949^**	0.945^**	0.363
Shannon指数Shannon index	-0.748^**	0.848^**	0.709^**	0.901^**	0.406	0.724^**	0.905^**	0.521
Simpson指数Simpson index	0.665^*	-0.752^**	-0.589^*	-0.836^**	-0.438	-0.607^*	-0.837^**	-0.550

Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region

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