Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects

doi:10.3969/j.issn.1004-1524.2021.05.13

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (5): 873-884.DOI: 10.3969/j.issn.1004-1524.2021.05.13

• Environmental Science • Previous Articles Next Articles

Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects

LI Fuyan(), LIU Xiaoyu, YAN jingting, CAI Yanfei^*()

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

Received:2020-10-25 Online:2021-05-25 Published:2021-05-25
Contact: CAI Yanfei

Abstract

Abstract:

In order to enrich the resources of strains with the function of indole-3-acetic acid (IAA) production, hence to develop high-efficiency plant-growth-promoting microbial fertilizer, three strains with high IAA production ability were isolated from the rhizosphere soil of maize, lettuce and green belt vegetation plant, and were encoded as YC9L, YC3172 and YC5064, respectively. The IAA production of YC9L, YC3172 and YC5064 reached 46.72, 79.75, 67.55 mg·L^-1, respectively. According to the characteristics and 16S rDNA identification, YC9L and YC3172 strains were identified as Bacillus massiliogorillae, and YC5064 strain was identified as Bacillus badius. The maize seed germination test showed that YC9L and YC5064 strains could promote seed germination and seedling growth of maize. The lettuce pot experiment showed that YC9L, YC3172, YC5064 strains had obvious promoting effect on the growth of lettuce. The maize pot experiment showed that YC9L, YC3172, YC5064 strains could promote the growth of maize. In addition, YC9L, YC3172 and YC5064 strains all had the ability to dissolve potassium. YC9L and YC3172 strains had the ability to dissolve phosphorus. YC9L, YC3172 and YC5064 strains had resistance to Cd, Zn and Cu.

Key words: indole-3-acetic acid, Bacillus, plant-growth-promoting rhizobacteria

CLC Number:

S154.39

LI Fuyan, LIU Xiaoyu, YAN jingting, CAI Yanfei. Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects[J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 873-884.

Figures/Tables 14

References 31

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处理Treatment	株高Plant height/cm	根长Root length/cm
CK	6.17±0.32 b	2.70±0.31 c
YC9L	7.74±0.48 a	8.18±0.52 a
YC5064	7.18±0.38 ab	4.86±0.33 b

处理Treatment	株高Plant height/cm	根长Root length/cm
CK	6.17±0.32 b	2.70±0.31 c
YC9L	7.74±0.48 a	8.18±0.52 a
YC5064	7.18±0.38 ab	4.86±0.33 b

处理 Treatment	株高 Plant height/cm	根长 Root length/cm	叶长 Leaf length/cm	茎粗 Stem thickness/mm	叶宽 Leaf width/cm	鲜重 Fresh weight/g
CK	9.19±0.38 b	10.81±0.55 b	8.41±0.36 b	2.87±0.05 c	5.34±0.24 b	3.25±0.27 b
YC9L	10.82±0.48 a	10.74±0.58 b	10.27±0.46 a	3.66±0.16 b	6.32±0.27 a	4.91±0.42 a
YC3172	10.69±0.39 a	13.06±0.56 a	10.23±0.37 a	3.55±0.09 ab	6.52±0.18 a	5.19±0.31 a
YC5064	11.06±0.31 a	11.17±0.59 b	9.91±0.47 a	3.90±0.09 a	6.33±0.24 a	5.09±0.34 a

处理 Treatment	株高 Plant height/cm	根长 Root length/cm	叶长 Leaf length/cm	茎粗 Stem thickness/mm	叶宽 Leaf width/cm	鲜重 Fresh weight/g
CK	9.19±0.38 b	10.81±0.55 b	8.41±0.36 b	2.87±0.05 c	5.34±0.24 b	3.25±0.27 b
YC9L	10.82±0.48 a	10.74±0.58 b	10.27±0.46 a	3.66±0.16 b	6.32±0.27 a	4.91±0.42 a
YC3172	10.69±0.39 a	13.06±0.56 a	10.23±0.37 a	3.55±0.09 ab	6.52±0.18 a	5.19±0.31 a
YC5064	11.06±0.31 a	11.17±0.59 b	9.91±0.47 a	3.90±0.09 a	6.33±0.24 a	5.09±0.34 a

处理 Treatment	株高 Plant height/cm	叶长 Leaf length/cm	根长 Root length/cm	茎粗 Stem thickness/mm	叶宽 Leaf width/cm	根干重 Root dry weight/g	叶干重 Leaf dry weight/g
CK	39.07±0.60 b	29.64±0.45 b	24.97±0.92 a	4.81±0.09 b	2.15±0.04 b	0.24±0.02 b	0.37±0.01 b
YC9L	41.55±0.61 a	31.85±0.45 a	27.30±0.90 a	5.73±0.15 a	2.37±0.06 a	0.34±0.03 a	0.46±0.02 a
YC3172	38.79±0.68 b	29.99±0.50 b	27.75±0.87 a	5.58±0.14 a	2.31±0.07 ab	0.29±0.02 ab	0.39±0.02 b
YC5064	39.97±0.46 ab	31.01±0.47 ab	26.67±1.68 a	5.40±0.11 a	2.33±0.06 a	0.28±0.01 b	0.41±0.01 ab

Isolation and identification of three indole-3-acetic acid producing plant-growth-promoting rhizosphere Bacillus sp. and their growth-promoting effects

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