Molecular marker and colonization of Plantibacter sp. WZW03

doi:10.3969/j.issn.1004-1524.2018.10.15

›› 2018, Vol. 30 ›› Issue (10): 1715-1721.DOI: 10.3969/j.issn.1004-1524.2018.10.15

• Plant Protection • Previous Articles Next Articles

Molecular marker and colonization of Plantibacter sp. WZW03

WANG Zhigang^{1, 2}, LIU Zeping¹, HU Yunlong¹, LIU Hong¹, ZHU Xiaohui¹

1. School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
2. Key Laboratory of Urban Agriculture in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China

Received:2018-01-17 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: In this study, the fluorescent plasmid POT2-RFP was transferred into Plantibacter sp. WZW03 as markers and the colonization of strain WZW03 was studied in watermelon rhizosphere. Transgenics with genetically stable transformants were successfully obtained in Plantibacter sp. WZW03-P, and growth characteristic of Plantibacter sp. WZW03-P was not significantly different from that of WZW03. Under the same bacterial concentration, WZW03-P had the largest adsorption capacity of (50.87±0.06)×10⁵ cfu·g^-1 when the watermelon root surface was adsorbed for 50 min. Under the same adsorption time, the adsorption capacity increased with the increase of the strain concentration and the saturation concentration of adsorption was (1.27±0.34)×10⁷ cfu·g^-1. The number of WZW03-P in sterilized soil and normal soil decreased with the prolonged feeding time, and the number of viable bacteria was stable at 10⁶ cfu·g^-1 at 20 d. The promoting effects of WZW03-P were found on the growth of watermelon plants and roots, and the colonization rate of WZW03-P in the watermelon rhizosphere was high, which showed that WZW03-P was provided with a significant competitive advantage in rhizosphere. In conclusion, Plantibacter sp. WZW03-P could colonize well in the watermelon rhizosphere, and promote the growth of watermelon.

Key words: molecular marker, plant growth-promoting rhizobacteria, watermelon

CLC Number:

S154.39

WANG Zhigang, LIU Zeping, HU Yunlong, LIU Hong, ZHU Xiaohui. Molecular marker and colonization of Plantibacter sp. WZW03[J]. , 2018, 30(10): 1715-1721.

References

[1] LIU K, NEWMAN M, MCINROY J A, et al.Selection and assessment of plant growth-promoting rhizobacteria for biological control of multiple plant diseases[J]. Phytopathology, 2017, 107(8): 928-936.
[2] YUAN J, ZHANG N, HUANG Q, et al.Organic acids from root exudates of banana help root colonization of PGPR strain Bacillus amyloliquefaciens NJN-6[J]. Scientific Reports, 2015, 5: 13438.
[3] 翁启勇, 陈庆河, 赵健, 等. 利福平标记菌株BS1在番茄、茄子根部及土壤中的定殖动态[J]. 福建农业学报, 2003, 18(2): 87-88.
WENG Q Y, CHEN Q H, ZHAO J, et al.Colonization of BS1 marked with rifampicin on roots and in root zone soil of tomato and eggplant[J]. Fujian Journal of Agricultural Sciences, 2003, 18(2): 87-88. (in Chinese with English abstract)
[4] BENEDUZI A, AMBROSINI A, PASSAGLIA L M.Plant growth-promoting rhizobacteria (PGPR): their potential as antagonists and biocontrol agents[J]. Genetics & Molecular Biology, 2012, 35(4):1044-1051.
[5] SINGH B P.Molecular and functional diversity of PGPR fluorescent Pseudomonads based on 16S rDNA-RFLP and RAPD markers[J]. Journal of Environmental Biology, 2015, 36(5):1169-1178.
[6] 常慧萍, 曹媛媛, 马忠友, 等. LuxAB标记的N2106-L在小麦根圈的定植动态[J]. 中国微生态学杂志, 2009, 21(3): 213-216.
CHANG H P, CAO Y Y, MA Z Y, et al.Colonization dynamics of LuxAB gene-marked N2106-L in wheat rhizosphere[J]. Chinese Journal of Microecology, 2009, 21(3): 213-216. (in Chinese with English abstract)
[7] 张楠, 吴凯, 沈怡斐, 等. 根际益生菌解淀粉芽孢杆菌SQR9在香蕉根表的定殖行为研究[J]. 南京农业大学学报, 2014, 37(6): 59-65.
ZHANG N, WU K, SHEN Y F, et al.Investigation of the colonization patterns of plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9 on banana roots[J]. Journal of Nanjing Agricultural University, 2014, 37(6): 59-65. (in Chinese with English abstract)
[8] 王志刚, 刘帅, 徐伟慧,等. 硅酸盐细菌的分离鉴定及其对西瓜的促生效应[J]. 核农学报, 2015, 29(11): 2208-2214.
WANG Z G, LIU S, XU W H, et al.The isolation and identification of a silicate bacterium and the promoting effects on watermelon[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(11): 2208-2214. (in Chinese with English abstract)
[9] 车路阳, 郭志兰, 郭清华, 等. 优化培养条件和裂解方法提高hRANKL在大肠杆菌内的表达[J]. 生物工程学报, 2017, 33(5): 849-862.
CHE L Y, GUO Z L, GUO Q H, et al.Enhancing hRANKL production in Escherichia coli by optimizing culture conditions and lysing program[J]. Chinese Journal of Biotechnology, 2017, 33(5): 849-862. (in Chinese with English abstract)
[10] 任如意, 薛巨坤, 国会艳, 等. 北玄参毛状根诱导及其植株再生[J]. 植物学报, 2017, 52(6): 783-787.
REN R Y, XUE J K, GUO H Y, et al.Induction of hairy roots of Scrophularia buergeriana and its plant regeneration[J]. Bulletin of Botany, 2017, 52(6): 783-787. (in Chinese with English abstract)
[11] 张石磊, 翟向和, 王春光, 等. 小檗碱对禽源大肠杆菌抑菌作用及耐药消除作用的转录组学分析[J]. 中国畜牧兽医, 2017, 44(5): 1518-1525.
ZHANG S L, ZHAI X H, WANG C G, et al.Study on antimicrobial effect of berberine sulfate on avian Escherichia coli and elimination of levofloxacin resistance based on transcriptome sequencing[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(5): 1518-1525. (in Chinese with English abstract)
[12] BABALOLA O O, OSIR E O, SANNI A I, et al.Amplification of 1-amino-cyclopropane-1-carboxylic (ACC) deaminase from plant growth promoting rhizobacteria in Striga-infested soil[J]. African Journal of Biotechnology, 2003, 2(6): 157-160.
[13] LEFF L G, LEFF A A.Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments[J]. Applied & Environmental Microbiology, 1996, 62(9):3486-3488.
[14] 车建美, 蓝江林, 刘波. 转绿色荧光蛋白基因的青枯雷尔氏菌生物学特性[J]. 中国农业科学, 2008, 41(11): 3626-3635.
CHE J M, LAN J L, LIU B.GFP tagging Ralstonia solanacearum with gfp/luxAB mini-Tn5[J]. Scientia Agricultura Sinica, 2008, 41(11): 3626-3635. (in Chinese with English abstract)
[15] ZHANG J, LIU J, MENG L, et al.Isolation and characterization of plant growth-promoting rhizobacteria from wheat roots by wheat germ agglutinin labeled with fluorescein isothiocyanate[J]. Journal of Microbiology, 2012, 50(2): 191-198.
[16] HOWIE W J, SUSLOW T V.Role of antibiotic biosynthesis in the inhibition of Pythium ultimum in the cotton spermosphere and rhizosphere by Pseudomonas fluorescens[J]. Molecular Plant-Microbe Interactions, 1991, 4: 393-399.
[17] 程美霞, 张建, 唐欣昀, 等. 黄单胞菌P5310-luxAB菌株在水稻根际的吸附与土壤中的存活[J]. 中国微生态学杂志, 2011, 23(7): 589-592.
CHENG M X, ZHANG J, TANG X Y, et al.The adsorption of Xanthomonas sp P5310-luxAB strain on rice roots and its survival in soils[J]. Chinese Journal of Microecology, 2011, 23(7): 589-592. (in Chinese with English abstract)
[18] NADEEM S M, AHMAD M, ZAHIR Z A, et al.The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments[J]. Biotechnology Advances, 2014, 32(2): 429-448.

Molecular marker and colonization of Plantibacter sp. WZW03

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Xinyue, LI Youfa, LIU Jiangning, FU Haowei. Study on hybrid rice purity of special combining pattern testing with functional marker of wide compatibility gene S5-n [J]. , 2020, 32(1): 15-19.
[2]	HONG Xianxian, WU Xiuqin, LUO Jinyan, WANG Yanli, SUN Guocang, AN Qianli, QIU Wen, LI Bin. Pathogen identification of bacterial fruit blotch of melon on sweet potato in Zhejiang Province [J]. , 2019, 31(7): 1112-1118.
[3]	HE Haiyan, CHAI Rongyao, QIU Haiping, MAO Xueqin, WANG Yanli, SUN Guocang. Distribution and resistance evaluation of 5 rice blast-resistant genes in cultivated rice varieties in Zhejiang [J]. , 2019, 31(6): 922-929.
[4]	WANG Zhangjun, LIU Yan, ZHANG Shuangxi, LIU Fenglou, LI Qingfeng, ZHANG Xiaogang, LIU Shengxiang, JIA Biao. Identification on disease resistance and molecular markers of F₂ hybrids from Ningchun No.4 and Hedong black wheat [J]. , 2019, 31(5): 677-687.
[5]	XU Weihui, LYU Zhihang, SHI Yiran, JIANG Jiaying, HU Yunlong, WANG Zhigang. Establishment of complex growth-promoting rhizobacteria for watermelon and promoting effect on watermelon roots [J]. , 2018, 30(5): 778-786.
[6]	WANG Guorong, HUA Wei, CHEN Gonghai, LONG Zhoukai, LI Bo, ZHANG Wenying, XU Yanhao. Genetic diversity in hulless barley by retrotransposons-based markers and simple sequence repeat (SSR) markers [J]. , 2018, 30(3): 357-365.
[7]	YANG Jing, ZHU Biao, HE Yong, YING Quansheng, ZHANG Leichen. Study on mechanical properties and fruit quality analysis of watermelon (Cirtullus lanatus) [J]. , 2017, 29(9): 1581-1588.
[8]	CHEN Yuansong, ZHU Xiaowei, GONG Xiangyu, MEI Zhicheng, ZHU Biao. Research advance of molecular marker-assisted selection in tomato disease resistance breeding in China [J]. , 2017, 29(8): 1415-1420.
[9]	YING Quansheng, HE Yong, WANG Yinger, CAO Tingting, GU Binquan, WANG Yuhong. Effects of grafting with different rootstocks on watermelon fruit quality [J]. , 2017, 29(4): 590-596.
[10]	XU Wei-hui, WU Feng-zhi. Response of soil enzymes activities and microorganism in rhizosphere of watermelon to wheat as companion crop [J]. , 2016, 28(9): 1588-1594.
[11]	LU Yong 1， 2， LI Zhenfeng 1， 2， *， PU Hongjie 1， 2， WANG Disong 1， 2. Determination of storage time of watermelon based on acoustic impulse method [J]. , 2016, 28(4): 682-.
[12]	QIU Zhennan 1，2， XU Qiankun1， WANG Xiaoqi2， ZHAO Juan2， HE Lei2， ZHANG Sen2， MA Bojun1， QIAN Qian2， ZHU Li 2,*. The development and verification of nucleotide polymorphism markers in rice [J]. , 2016, 28(3): 361-.
[13]	WANG Mingyou， ZHANG Hong， LI Shiping， JING Dawei. Responses of photosynthetic characteristics and growth of watermelon at the initial fructicative period to nitrogen deficiency in different degree [J]. , 2016, 28(3): 457-.
[14]	WANG Di-song, LI Zhen-feng1, PU Hong-jie, LU Yong. Study on forecast method for watermelon growth period based on vibration [J]. , 2016, 28(11): 1941-1946.
[15]	ZHAO Xiao\\|qiang， NIU Xiao\\|wei， FAN Min*. Influencing factors and research progress on transformation of watermelon by Agrobacterium [J]. , 2016, 28(1): 171-.