Screening of cellulase producing strains from rotten wood in Xinjiang cold area and analysis of their characteristics of enzyme production at low temperature

doi:10.3969/j.issn.1004-1524.2021.08.14

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (8): 1468-1476.DOI: 10.3969/j.issn.1004-1524.2021.08.14

• Environmental Science • Previous Articles Next Articles

Screening of cellulase producing strains from rotten wood in Xinjiang cold area and analysis of their characteristics of enzyme production at low temperature

FENG Xinxin¹(), LI Fenglan¹, XU Yongqing¹, LI Lei¹, HE Fumeng¹, FENG Yanzhong², YUAN Qiang¹^,^*(), LIU Di²^,^*()

1. School of Life Sciences, Northeast Agricultural University, Harbin 150030, China
2. Heilongjiang Academy of Agricultural Sciences, Harbin 150030, China

Received:2020-09-23 Online:2021-08-25 Published:2021-08-27
Contact: YUAN Qiang,LIU Di

Abstract

Abstract:

In order to screen out the strains which can degrade cellulose efficiently at low temperature and improve the degradation rate of cellulose under low temperature, in this study, rotten wood in cold area of Xinjiang was used as experimental material, and the low-temperature cellulose degrading bacteria were screened. Four strains of fungi which could grow at low temperature and degrade cellulose were screened at 4 ℃. Morphological and molecular biological methods were used for identification of low temperature strains. They were Penicillium chrysogenum, two Trichoderma citrinoviride strains and Neurospora sitophila. The cold tolerance test showed that all the strains were cold resistant. Studies on the characteristics of enzyme production of 4 low-temperature bacteria showed that the optimal culture time for cellulase production was 9 d, the optimal initial pH of the culture medium was 7, the optimal temperature was 25 ℃, and the optimal inoculation amount was 5%. The straw degradation test showed that these four strains of fungi had ability to degrade the straw, and had the best degradation effect on the corn straw with the fermentation rate above 40%.

Key words: cellulose, degrading bacteria, low temperature, strain identification, straw degradation

CLC Number:

S662.2

FENG Xinxin, LI Fenglan, XU Yongqing, LI Lei, HE Fumeng, FENG Yanzhong, YUAN Qiang, LIU Di. Screening of cellulase producing strains from rotten wood in Xinjiang cold area and analysis of their characteristics of enzyme production at low temperature[J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1468-1476.

Figures/Tables 16

Fig.1 Rotten trunk of spruce trees

Table 1 Screening results of low temperature cellulase producing strains

菌株编号 No. of strains	培养温度Culture temperature/℃
菌株编号 No. of strains	4	10	15	25	37
F1	+	+	+	+	+
F2	+	+	+	+	+
F3	-	-	+	+	-
F4	-	-	+	+	+
F5	-	+	+	+	+
D1	+	+	+	+	+
D2	-	-	+	+	+
D3	-	-	+	+	+
D4	+	+	+	+	+
D7	-	-	+	+	+
P1	+	+	+	+	+
P2	-		+	+	+
P4	+	+	+	+	+
N3	-	-	+	+	-
N5	+	+	+	+	+
N7	-	-	+	+	-
T2	+	+	+	+	+
T5	-	-	+	+	+
T6	-	-	+	+	+
T7	+	+	+	+	+
T8	+	+	+	+	+

Fig.2 Transparent circle of cellulose producing fungi on CMC-Congo red medium

Fig.3 Colony morphology of the cellulose producing fungi

Fig.4 Microscopic morphology of the cellulose producing fungi

Fig.5 Phylogenetic tree of strain P1 based on 18S rDNA sequences homology

Fig.6 Phylogenetic tree of strain T2 based on 18S rDNA sequences homology

Fig.7 Phylogenetic tree of strain T8 based on 18S rDNA sequences homology

Fig.8 Phylogenetic tree of strain N5 based on 18S rDNA sequences homology

Fig.9 Effect of differernt nitrogen fountains on cellulase production of four strains The different lowercase letters indicate significant differences among treatments (P<0.05). The same as below.

Fig.10 Effect of different inoculum on cellulase production of four strains

Fig.11 Effect of different temperature on cellulase production of four strains

Fig.12 Effect of initial pH on cellulase production of four strains

Fig.13 Effect of incubating time on cellulase production of four strains

Fig.14 Weight loss rate of corn straw fermented by four fungal strains

Fig.15 Weight loss rate of different straws fermented by four fungal strains

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Screening of cellulase producing strains from rotten wood in Xinjiang cold area and analysis of their characteristics of enzyme production at low temperature

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