Optimization of Chinese medicine plant extract compounds with orthogonal design

doi:10.3969/j.issn.1004-1524.2021.12.09

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2295-2303.DOI: 10.3969/j.issn.1004-1524.2021.12.09

• Animal Science • Previous Articles Next Articles

Optimization of Chinese medicine plant extract compounds with orthogonal design

TAO Xin(), WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei^*()

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-06-28 Online:2021-12-25 Published:2022-01-10
Contact: XU Ziwei

Abstract

Abstract:

To prepare Chinese medicine plant extracts replacing feed antibiotics. Firstly, Grana Pericarpium, Cinnamomi Ramulus, Magnoliae officinalis cortex, Artemisiae annuae herba, Spatholobi caulis were sieved from 19 Chinese medicine plants as materials of extract compounds by examining antibacterial rate, inhibitory rateanti-inflammatory rate, and antioxidant IC₅₀ value as evaluating index. Secondly, proportions of five materials were optimized by a L₁₆ (4 ⁵) orthogonal experiment,by range analysis of results, three extract compounds with the strongest antibacterial (F_ab), anti-inflammatory (F_ai) and antioxidant (F_ao) activities were obtained. These material ratios of Magnoliae officinalis cortex, Spatholobi caulis, Artemisiae annuae herba, Cinnamomi Ramulus and Grana Pericarpium were 7∶13∶6∶9∶1 in F_ab, 7∶13∶4∶1∶1 in F_ai, and 3∶13∶4∶1∶7 in F_ao, respectively. Finally, the verifying results showed that the minimum inhibitory concentration of F_ab was 125 mg·mL^-1, the anti-inflammatory IC₅₀ value of F_ai was 0.144 mg·mL^-1, and the antioxidant IC₅₀ value of F_ao was 0.222 mg·mL^-1. In summary, the present study obtained the best three Chinese medicine extract compounds with stronger antibacterial, anti-inflammatory and antioxidant effects, respectively.

Key words: Chinese medicine, orthogonal design, plant extract compounds, antibacterial, anti-inflammatory, antioxidant

CLC Number:

S853.7

TAO Xin, WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei. Optimization of Chinese medicine plant extract compounds with orthogonal design[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2295-2303.

Figures/Tables 9

References 19

[1]	ABREU A C, MCBAIN A J, SIMÕES M. Plants as sources of new antimicrobials and resistance-modifying agents[J]. Natural Product Reports, 2012, 29(9): 1007-1021. DOI URL
[2]	TAO X, SUN Y Q, MEN X M, et al. A compound plant extract and its antibacterial and antioxidant properties in vitro and in vivo[J]. 3 Biotech, 2020, 10(12): 1-10. DOI URL
[3]	陶新, 孙雨晴, 门小明, 等. 复合植物提取物替代饲用抗生素对断奶仔猪生长性能和血清指标的影响[J]. 动物营养学报, 2018, 30(1): 163-169.
	TAO X, SUN Y Q, MEN X M, et al. Effects of composite plant extracts replacing feed antibiotics on growth performance and serum indexes of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2018, 30(1): 163-169.(in Chinese with English abstract)
[4]	周子雄, 黄庆华, 朱爽, 等. 酶标仪快速测定抗菌物质抑菌活性方法的建立[J]. 微生物前沿, 2014, 3(2): 29-35.
	ZHOU Z X, HUANG Q H, ZHU S, et al. Establishment of rapid determining method for antibacterial activity by microplate reader[J]. Advances in Microbiology, 2014, 3(2): 29-35. (in Chinese with English abstract) DOI URL
[5]	GÜLLÜCE M, SÖKMEN M, DAFERERA D, et al. In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L[J]. Journal of Agricultural and Food Chemistry, 2003, 51(14): 3958-3965. DOI URL
[6]	BANERJEE S. Inhibition of mackerel (Scomber scombrus) muscle lipoxygenase by green tea polyphenols[J]. Food Research International, 2006, 39(4): 486-491. DOI URL
[7]	EL-GHORAB A H, MAHGOUB M H, BEKHETA M. Effect of some bioregulators on the chemical composition of essential oil and its antioxidant activity of Egyptian carnation (Dianthus caryophyllus L.)[J]. Journal of Essential Oil Bearing Plants, 2006, 9(3): 214-222. DOI URL
[8]	HUSSAIN T, TAN B, YIN Y L, et al. Oxidative stress and inflammation: what polyphenols can do for us?[J]. Oxidative Medicine and Cellular Longevity, 2016, 2016: 7432797.
[9]	OZDAL T, SELA D A, XIAO J B, et al. The reciprocal interactions between polyphenols and gut microbiota and effects on bioaccessibility[J]. Nutrients, 2016, 8(2): 78. DOI URL
[10]	BAHARFAR R, AZIMI R, MOHSENI M. Antioxidant and antibacterial activity of flavonoid-, polyphenol-and anthocyanin-rich extracts from Thymus kotschyanus boiss & hohen aerial parts[J]. Journal of Food Science and Technology, 2015, 52(10): 6777-6783. DOI URL
[11]	黄其春, 黄翠琴, 陈彤, 等. 银杏叶提取物对断奶仔猪生长性能、肠黏膜形态及屏障通透性的影响[J]. 中国畜牧杂志, 2014, 50(19): 74-77.
	HUANG Q C, HUANG C Q, CHEN T, et al. Effects of Ginkgo biloba extract on growth performance, intestinal mucosal morphology and barrier permeability in weaned piglets[J]. Chinese Journal of Animal Science, 2014, 50(19): 74-77.(in Chinese with English abstract)
[12]	LIU G, GUAN G P, FANG J, et al. Macleaya cordata extract decreased diarrhea score and enhanced intestinal barrier function in growing piglets[J]. BioMed Research International, 2016, 2016: 1069585.
[13]	HAO Y, LI D F, PIAO X L, et al. Forsythia suspensa extract alleviates hypersensitivity induced by soybean β-conglycinin in weaned piglets[J]. Journal of Ethnopharmacology, 2010, 128(2): 412-418. DOI URL
[14]	韩杰, 张飞, 边连全. 刺五加多糖对免疫应激断奶仔猪免疫器官指数、粪便微生物菌群数量和胃肠道pH的影响[J]. 动物营养学报, 2014, 26(8): 2314-2319.
	HAN J, ZHANG F, BIAN L Q. Effects of Acanthopanax senticosus polysaccharide on immune organ indexes, counts of fecal microflora and gastrointestinal pH of immune challenged weaner piglets[J]. Chinese Journal of Animal Nutrition, 2014, 26(8): 2314-2319.(in Chinese with English abstract)
[15]	MALVIYA S, ARVIND, JHA A, et al. Antioxidant and antibacterial potential of pomegranate peel extracts[J]. Journal of Food Science and Technology, 2014, 51(12): 4132-4137. DOI URL
[16]	MASTROGIOVANNI F, MUKHOPADHYA A, LACETERA N, et al. Anti-inflammatory effects of pomegranate peel extracts on in vitro human intestinal caco-2 cells and ex vivo porcine colonic tissue explants[J]. Nutrients, 2019, 11(3): E548.
[17]	孙雨晴. 复合植物提取物的组方、安全性评价及其在小鼠上的应用研究[D]. 金华: 浙江师范大学, 2012.
	SUN Y Q. The components, safety evaluation, and application study on mouse of the compound plant extracts[D]. Jinhua: Zhejiang Normal University, 2012. (in Chinese with English abstract)
[18]	朱心红, 沈群, 高天明. 中药成分组合效应假说及实验研究[J]. 中草药, 2004, 35(2): 122-124.
	ZHU X H, SHEN Q, GAO T M. Verification on hypojournal for optimum ratio in various active constituents of Chinese materia medica with desired effects[J]. Chinese Traditional and Herbal Drugs, 2004, 35(2): 122-124.(in Chinese with English abstract)
[19]	刘岳, 李梦云, 郑寅, 等. 复方头孢泊肟酯纳米乳的研制及其药效与安全性研究[J]. 畜牧兽医学报, 2015, 46(6): 1026-1036.
	LIU Y, LI M Y, ZHENG Y, et al. Preparation, efficacy and safety evaluation of compound nanoemulsion of cefpodoxime proxetil[J]. Chinese Journal of Animal and Veterinary Sciences, 2015, 46(6): 1026-1036.(in Chinese with English abstract)

水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

因素 Factor	抑菌率 Antibacterial rate						抗炎率 Anti-inflammatory rate						抗氧化IC50值 Antioxidant IC50 value
因素 Factor	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ (mg· mL^-1)	k₂/ (mg· mL^-1)	k₃/ (mg· mL^-1)	k₄/ (mg· mL^-1)	R/ (mg· mL^-1)	最优水平 The best level
A	28.54	28.76	56.77	60.28	31.74	4	77.50	76.11	84.61	86.79	9.929	4	0.679	0.435	0.835	0.587	0.4	2
B	45.26	43.36	29.29	56.44	27.15	4	72.43	80.75	81.14	89.93	17.50	4	0.531	0.838	0.647	0.519	0.319	4
C	36.56	55.34	33.88	48.57	21.46	2	84.79	80.48	78.93	80.05	5.857	1	0.536	0.618	0.587	0.794	0.258	1
D	40.13	35.41	46.71	52.1	16.69	4	84.82	81.99	79.00	78.45	6.375	1	0.452	0.535	0.927	0.621	0.475	1
E	58.38	45.42	45.31	25.23	33.15	1	91.62	82.03	77.22	73.39	18.22	1	1.153	0.688	0.377	0.317	0.836	4

Optimization of Chinese medicine plant extract compounds with orthogonal design

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变异来源	自由度	均方	F值	P值
Sources of variation	Degree of freedom	Mean square	F value	P value
抑菌率 Antibacterial rate
A	3	3 594.26	126.06	<0.001
B	3	894.22	31.36	<0.001
C	3	1 226.28	43.01	<0.001
D	3	1 438.99	50.47	<0.001
E	3	2 834.09	99.40	<0.001
误差Error	32	28.51	—	—
抗炎率 Anti-inflammatory rate
A	3	298.33	114.46	<0.001
B	3	574.09	220.26	<0.001
C	3	79.14	30.36	<0.001
D	3	65.26	25.04	<0.001
E	3	577.70	221.64	<0.001
误差Error	32	2.606	—	—
抗氧化IC₅₀值 Antioxidant IC₅₀ value
A	3	0.336	318.64	<0.001
B	3	0.114	108.29	<0.001
C	3	0.150	142.26	<0.001
D	3	0.586	555.44	<0.001
E	3	1.458	1 383.4	<0.001
误差Error	32	0.001	—	—

因素 Factor	水平 Level	评价指标Evaluation index
因素 Factor	水平 Level	抑菌率 Antibacterial rate%	抗炎率 Anti-inflammatory rate%	抗氧化IC50值 Antioxidant IC50 value/(mg·mL^-1)
A	1	28.54±1.541 b	77.50±0.446 b	0.679±0.009 b
	2	28.76±1.541 b	76.11±0.446 c	0.435±0.009 b
	3	56.77±1.541 a	87.11±0.446 a	0.834±0.009 d
	4	60.28±1.541 a	86.04±0.446 a	0.586±0.009 c
B	1	40.26±1.748 b	72.08±0.528 c	0.577±0.011 c
	2	40.13±1.748 b	80.49±0.528 b	0.753±0.011 a
	3	37.51±1.748 b	81.75±0.528 b	0.685±0.011 b
	4	56.44±1.541 a	89.93±0.466 a	0.519±0.009 d
C	1	36.56±1.541 c	84.79±0.466 a	0.536±0.009 c
	2	63.57±2.101 a	81.09±0.635 b	0.656±0.013 b
	3	30.65±2.101 d	78.67±0.635 c	0.503±0.013 c
	4	43.57±2.101 b	79.70±0.635 bc	0.840±0.013 a
D	1	36.90±1.748 c	84.56±0.528 a	0.368±0.011 d
	2	30.41±1.748 d	81.64±0.528 b	0.581±0.011 c
	3	46.71±1.541 b	79.00±0.466 c	0.927±0.009 a
	4	60.33±1.748 a	79.05±0.528 c	0.659±0.011 b
E	1	66.61±1.748 a	92.22±0.528 a	1.191±0.011 a
	2	45.42±1.541 b	82.03±0.466 b	0.688±0.009 b
	3	40.31±1.748 c	76.87±0.528 c	0.423±0.011 c
	4	22.00±1.748 d	73.14±0.528 d	0.233±0.011 d

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