Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

doi:10.3969/j.issn.1004-1524.2021.11.11

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (11): 2085-2097.DOI: 10.3969/j.issn.1004-1524.2021.11.11

• Horticultural Science • Previous Articles Next Articles

Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

WANG Xian(), LIU Fang, WEI Xiaohong^*(), ZHU Xiaolin, WANG Baoqiang

College of Life Science and Technology, Gansu Agricultural University, Gansu Key Lab of Crop Genetic & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China

Received:2020-08-22 Online:2021-11-25 Published:2021-11-26
Contact: WEI Xiaohong

Abstract

Abstract:

Different tomato materials were inoculated with tomato yellow leaf curl virus (TYLCV) by Agrobacterium tumefaciens infiltration. Real-time quantitative PCR and physiological and biochemical methods were used to evaluate the resistance to TYLCV. The phenotypic characteristics, virus carrying rate, antioxidant enzyme activity, secondary metabolite content and resistance gene expression of tomato materials with different resistance were analyzed. The results showed that the virus infection rates of tomato materials were 100%; the tested variety 853 had no symptoms, while 802 and 803 were the most susceptible; the varieties 867, 857 and 853 had higher antioxidant enzyme activity, contents of total phenols and flavonoids, and higher resistance gene expression, followed by 817 and 842, the lowest was 803. SOD activity, CAT activity, APX activity, total phenol content, flavonoid content, Ty-1 gene, Ty-2 and Ty-3 gene expression levels were significantly positively correlated with each other (total phenol and Ty-1 showed significantly positive correlation, P<0.01). Compared with other disease resistance genes, the expression of Ty-1 was delayed after tomato plants were infected with TYLCV. The resistance genes, antioxidant enzymes, total phenols and flavonoids in plants were significantly positively correlated, which jointly improved the resistance of tomato plants to TYLCV.

Key words: tomato, tomato yellow leaf curl virus disease, antioxidant enzymes, secondary metabolites, resistance gene, resistance

CLC Number:

S641.2

WANG Xian, LIU Fang, WEI Xiaohong, ZHU Xiaolin, WANG Baoqiang. Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2085-2097.

Figures/Tables 13

References 36

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编号 Number	基因型 Genotype	编号 Number	基因型 Genotype
801	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	822	ty-1/ty-1+ty-3/ty-3
802	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	841	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
803	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	842	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
805	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	853	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
806	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	857	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
817	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	867	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
818	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3	齐达利Qidali	Ty-1/Ty-1+ty-2/ty-2+Ty-3a/ty-3a
819	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	MM	无
820	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3

编号 Number	基因型 Genotype	编号 Number	基因型 Genotype
801	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	822	ty-1/ty-1+ty-3/ty-3
802	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	841	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
803	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	842	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3
805	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	853	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
806	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	857	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
817	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	867	Ty-1/Ty-1+ty-2/ty-2+Ty-3/Ty-3
818	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3	齐达利Qidali	Ty-1/Ty-1+ty-2/ty-2+Ty-3a/ty-3a
819	ty-1/ty-1+ty-2/ty-2+ty-3/ty-3	MM	无
820	Ty-1/Ty-1+ty-2/ty-2+ty-3/ty-3

基因 Gene	引物序列 Primer sequence(5'-3')
Ty-1	F:GGCAAAATATGCAGCCAGGCTTTCC R:TCAGTATGTATACGAGGTTCGCCGT
Ty-2	F:TGCGGCTGTTTCCTATGGTT R:TGGTTCTGGAAAGGGATCGAA
Ty-3	F:TGAGCGGAGAAATGTGACAG R:CGGGGCTTTGATGAAACGAA
actin	F:GAAATAGCATAAGATGGCAGACG R:ATACCCACCATCACACCAGTAT

基因 Gene	引物序列 Primer sequence(5'-3')
Ty-1	F:GGCAAAATATGCAGCCAGGCTTTCC R:TCAGTATGTATACGAGGTTCGCCGT
Ty-2	F:TGCGGCTGTTTCCTATGGTT R:TGGTTCTGGAAAGGGATCGAA
Ty-3	F:TGAGCGGAGAAATGTGACAG R:CGGGGCTTTGATGAAACGAA
actin	F:GAAATAGCATAAGATGGCAGACG R:ATACCCACCATCACACCAGTAT

	SOD	CAT	APX	总酚 Total phenols	类黄酮 Flavonoids	Ty-1	Ty-2	Ty-3
SOD	1
CAT	0.757^**	1
APX	0.867^**	0.690^**	1
总酚Total phenols	0.802^**	0.699^**	0.739^**	1
类黄酮Flavonoids	0.702^**	0.797^**	0.682^**	0.798^**	1
Ty-1	0.635^**	0.658^**	0.568^**	0.356^*	0.527^**	1
Ty-2	0.734^**	0.753^**	0.702^**	0.631^**	0.705^**	0.698^**	1
Ty-3	0.754^**	0.762^**	0.703^**	0.631^**	0.786^**	0.732^**	0.822^**	1

Study on resistance of different tomato germplasm materials to yellow leaf curl virus disease

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