Effects of sugar content on resistance of tomato leaf to bacterial leaf spot and possible underlying mechanisms

doi:10.3969/j.issn.1004-1524.2021.06.05

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (6): 1001-1011.DOI: 10.3969/j.issn.1004-1524.2021.06.05

• Horticultural Science • Previous Articles Next Articles

Effects of sugar content on resistance of tomato leaf to bacterial leaf spot and possible underlying mechanisms

ZHAO Hu(), ZHANG Yueting, LIU Yonghua^*()

College of Horticulture, Hainan University, Haikou 570228, China

Received:2021-01-30 Online:2021-06-25 Published:2021-06-25
Contact: LIU Yonghua

Abstract

Abstract:

The study was conducted to study the effect of sugar metabolism on the resistance of tomato to bacterial leaf spot disease (Pst DC3000) and the physiological and biochemical mechanisms possibly involved, which could benefit the breeding of new tomato varieties with high resistance to Pst DC3000. To get leaves with different characteristics in sugar metabolism,tomato leaves were sampled at two different time (8:00 AM and 6:00 PM), which were referred to as morning-sampled leaves and evening-sampled leaves, respectively. These two kinds of leaves were in vitro inoculated with Pst DC3000 and then tested at 0, 24 and 48 h after inoculation for several indicators, including Pst DC3000 resistance, bacterial density, the content of soluble sugar and starch, invertase activities, the content of salicylic acid (SA) and jasmonic acid (JA), cell death incidence and H₂O₂ accumulation. It was found that, as compared with the morning-sampled leaves, the evening-sampled leaves showed higher resistance to Pst DC3000 as revealed by less incidence of disease lesion and cell death, and lower bacterial density in leaves (P≤0.01). Furthermore, evening-sampled leaves had higher starch content (0-48 h), glucose content (0 h and 24 h), and fructose content (24 h and 48 h). However, there was no significant difference in sucrose content between two kinds of leaves. In addition, the measurement of three kinds of invertase activities showed that evening-sampled leaves had lower cell wall invertase (CWIN) activity at 0 h and 48 h after inoculation, but significantly (P≤0.05) higher cytoplasmic invertase (CIN) activities at 24 h and 48 h after inoculation than morning-sampled leaves. Notably, there was no significant difference in vacuolar invertase (VIN) activity between two kinds of leaves, Finally, as compared with the morning-sampled leaves, evening-sampled leaves showed significantly (P≤0.05)higher content of free SA and JA, and simultaneously less H₂O₂ accumulation at 48 h after inoculation. In conclusion, as compared with the morning-sampled leaves, evening-sampled leaves showed higher starch and hexose content, CIN activity, but lower CWIN activity. These differences in sugar metabolism may contribute to higher SA and JA content and less H₂O₂ accumulation and cell death in evening-sampled leaves, and ultimatly enhance its resistance to Pst DC3000.

Key words: tomato, bacterial leaf spot, cell wall invertase, cytoplasmic invertase, sugar content

CLC Number:

S641.2

ZHAO Hu, ZHANG Yueting, LIU Yonghua. Effects of sugar content on resistance of tomato leaf to bacterial leaf spot and possible underlying mechanisms[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1001-1011.

Figures/Tables 5

Fig.1 Dynamic changes in disease lesion, cell death and bacteria density in tomato leaves at 0, 24 and 48 h after inoculation with Pst DC3000 All scale bars in A and B represented 1.25 cm; Arrows in B indicated blue signals of cell death; ** in C indicated significant (P≤0.01) differences between morning- and evening-sampled leaves.

Fig.2 The differences of morning- and evening-sampled leaves in in situ starch staining and starch content after inoculated with Pst DC3000 All scale bars represented 1.25 cm in A; ** in B indicated significant(P≤0.01) differences between morning- and evening-sampled leaves.

Fig.3 Differences of morning- and evening-sampled leaves in soluble sugar content and hexose/sucrose ratio after inoculated with Pst DC3000 * and ** indicated significant differences between morning- and evening-sampled leaves at 0.05 and 0.01 levels, respectively. The same as below.

Fig.4 Differences of morning- and evening-sampled leaves in invertase activities after inoculated with Pst DC3000

Fig.5 Differences of morning- and evening-sampled leaves in in situ H2O2 staining and the content of SA and JA after inoculated with Pst DC3000 All scale bars in A represented 1.25 cm; * and ** in B indicated significant differences between morning- and evening-sampled leaves at 0.05 and 0.01 levels, respectively.

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Effects of sugar content on resistance of tomato leaf to bacterial leaf spot and possible underlying mechanisms

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