Isolation, identification and control of rabbit epidemic abdominal distension

doi:10.3969/j.issn.1004-1524.2021.11.05

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (11): 2034-2040.DOI: 10.3969/j.issn.1004-1524.2021.11.05

• Animal Science • Previous Articles Next Articles

Isolation, identification and control of rabbit epidemic abdominal distension

JI Quan’an(), LIU Yan, XIAO Chenwen, HUANG Ye’e, LI Ke, WEI Qiang, BAO Guolian^*()

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-15 Online:2021-11-25 Published:2021-11-26
Contact: BAO Guolian

Abstract

Abstract:

In order to effectively control the spread of epidemic abdominal distension in rabbits, epidemiological investigation and laboratory differential diagnosis technology were applied in the present study. Pathogen detection was carried out on 112 cases of 40-90-d-old diseased rabbits. Among them, Escherichia coli and Clostridium welchii were isolated and identified from 78 and 69 diseased rabbits, respectively. The pathogenic characteristics test showed that these two species were the main pathogens of epidemic abdominal distension in rabbits. The clinical control trials of lysozyme were carried out in 3 rabbit farms suffering from epidemic abdominal distension. It was shown that either the incidence rate in the prevention trial or the mortality rate in the control trial was lowered by 50 percentage points in the treatment group than that in the control group. The results indicated that lysozyme could prevent and treat the disease, hence effectively curb the spread of the disease. Therefore, it could be used as the substitution of antibiotics. These findings provided scientific basis for the prevention and treatment of rabbit epidemic abdominal distension.

Key words: rabbit, epidemic abdominal distension, bacterial isolation, lysozyme

CLC Number:

S858.291

JI Quan’an, LIU Yan, XIAO Chenwen, HUANG Ye’e, LI Ke, WEI Qiang, BAO Guolian. Isolation, identification and control of rabbit epidemic abdominal distension[J]. Acta Agriculturae Zhejiangensis, 2021, 33(11): 2034-2040.

Figures/Tables 6

References 21

[1]	胡波, 范志宇, 王芳, 等. 患兔流行性腹胀病家兔肠道菌群结构的ERIC-PCR指纹图谱分析[J]. 安徽农业科学, 2016, 44(1):83-85.
	HU B, FAN Z Y, WANG F, et al. ERIC-PCR fingerprint of intestinal microflora of epidemic abdominal distention disease in domestic rabbit[J]. Journal of Anhui Agricultural Sciences, 2016, 44(1):83-85.(in Chinese with English abstract)
[2]	薛家宾, 尹秀凤, 周勇岐, 等. 兔流行性腹胀病[J]. 中国养兔杂志, 2008(1):26-28.
	XUE J B, YIN X F, ZHOU Y Q, et al. Epidemic abdominal distension in rabbits[J]. Chinese Journal of Rabbit Farming, 2008(1):26-28.(in Chinese)
[3]	坤清芳, 耿毅, 邓钊宾, 等. 家兔流行性腹胀病病因及其防治措施[J]. 黑龙江畜牧兽医, 2015(14):142-144.
	KUN Q F, GENG Y, DENG Z B, et al. The causes with prevention and treatment measures of epidemic abdominal distention disease in rabbits[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015(14):142-144.(in Chinese with English abstract)
[4]	林毅, 罗宜熟, 谢晶, 等. 家兔“流行性腹胀病”的病因和防治研究初报[J]. 中国养兔杂志, 2008(11):27-30.
	LIN Y, LUO Y S, XIE J, et al. A preliminary study on etiopathogenisis and prevention of rabbit epidemic abdominal distention[J]. Chinese Journal of Rabbit Farming, 2008(11):27-30.(in Chinese with English abstract)
[5]	EPAUD R, DELESTRAIN C, WEAVER T E, et al. Bacterial killing is enhanced by exogenous administration of lysozyme in the lungs[J]. Respiratory Medicine and Research, 2019, 76:22-27. DOI URL
[6]	张士璀, 许娜. 动物型溶菌酶研究新进展[J]. 中国海洋大学学报(自然科学版), 2014, 44(6):46-51.
	ZHANG S C, XU N. Advance in study of animal lysozymes[J]. Periodical of Ocean University of China, 2014, 44(6):46-51.(in Chinese with English abstract)
[7]	俞伟辉, 陈新风, 郭荣富, 等. 溶菌酶及其在动物生产中的应用前景[J]. 江西饲料, 2007(1):1-4.
	YU W H, CHEN X F, GUO R F, et al. Lysozyme and its application prospect in animal production[J]. Jiangxi Feed, 2007(1):1-4.(in Chinese)
[8]	王晓梅. 家兔流行性腹胀病病因、分析及防控措施[J]. 养殖技术顾问, 2010(9):113.
	WANG X M. Etiology, analysis and control measures of epidemic abdominal distension in rabbits[J]. Technical Advisor for Animal Husbandry, 2010(9):113.(in Chinese)
[9]	刘照波, 李明勇, 刘曼, 等. 一例兔大肠杆菌与魏氏梭菌混合感染病例诊断与药敏试验[J]. 中国养兔, 2020(2):4-6.
	LIU Z B, LI M Y, LIU M, et al. Diagnose and tests for drug susceptibility of a case of mixed infection of Escherichia coli and Clostridium yerticilli in rabbits[J]. Chinese Journal of Rabbit Farming, 2020(2):4-6.(in Chinese with English abstract)
[10]	白国勇, 刘向安, 张杰. 家兔流行性腹胀病病因及防治[J]. 四川畜牧兽医, 2010, 37(12):57-58.
	BAI G Y, LIU X A, ZHANG J. Etiology and prevention of epidemic abdominal distension in rabbits[J]. Sichuan Animal & Veterinary Sciences, 2010, 37(12):57-58.(in Chinese)
[11]	LICOIS D, COUDER T P, MARLIER D. Epizootic rabbit enteropathy[M]// MAERTENS L,COUDER T P. Recent advances in rabbit sciences. Melle, Belgium: ILVO, 2006: 164-165.
[12]	HUYBENS N, HOUEIX J, LICOIS D, et al. Pyrosequencing of epizootic rabbit enteropathy inocula and rabbit caecal samples[J]. The Veterinary Journal, 2013, 196(1):109-110. DOI URL
[13]	DJUKOVIC A, GARCIA-GARCERA M, MARTÍNEZ-PAREDES E, et al. Gut colonization by a novel Clostridium species is associated with the onset of epizootic rabbit enteropathy[J]. Veterinary Research, 2018, 49(1):1-14. DOI URL
[14]	PUÓN-PELÁEZ X H D, MCEWAN N R, GÓMEZ-SOTO J G, et al. Metataxonomic and histopathological study of rabbit epizootic enteropathy in Mexico[J]. Animals, 2020, 10(6):936. DOI URL
[15]	SZALO I M, LASSENCE C, LICOIS D, et al. Fractionation of the reference inoculum of epizootic rabbit enteropathy in discontinuous sucrose gradient identifies aetiological agents in high density fractions[J]. The Veterinary Journal, 2007, 173(3):652-657. DOI URL
[16]	杨加琼. 四川地区断奶仔兔腹泻、腹胀病的病原调查[J]. 四川畜牧兽医, 2015, 42(4):27-28.
	YANG J Q. Pathogen investigation of diarrhea and abdominal distension of weaned rabbits in Sichuan area[J]. Sichuan Animal & Veterinary Sciences, 2015, 42(4):27-28.(in Chinese)
[17]	THYMANN T, SØRENSEN K U, HEDEMANN M S, et al. Antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure in weanling pigs[J]. British Journal of Nutrition, 2007, 97(6):1128-1137. DOI URL
[18]	张世卿, 朱忠珂, 王明成, 等. 玉米-豆粕日粮添加溶菌酶对肉仔鸡生长性能、代谢及免疫指标的影响[J]. 动物营养学报, 2008, 20(4):463-468.
	ZHANG S Q, ZHU Z K, WANG M C, et al. Effects of lysozyme on growth performance, metabolism and immune indices of broilers fed with corn-soybean basal diets[J]. Chinese Journal of Animal Nutrition, 2008, 20(4):463-468. (in Chinese with English abstract)
[19]	赵荣文, 谭丽萍, 刘同军. 溶菌酶及其应用研究进展[J]. 齐鲁工业大学学报, 2021, 35(1):12-18.
	ZHAO R W, TAN L P, LIU T J. Research progress of lysozyme and its application[J]. Journal of Qilu University of Technology, 2021, 35(1):12-18.(in Chinese with English abstract)
[20]	OLIVER W T, WELLS J E, MAXWELL C V. Lysozyme as an alternative to antibiotics improves performance in nursery pigs during an indirect immune challenge[J]. Journal of Animal Science, 2014, 92(11):4927-4934. DOI URL
[21]	MAY K D, WELLS J E, MAXWELL C V, et al. Granulated lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology of 10-day-old pigs[J]. Journal of Animal Science, 2012, 90(4):1118-1125. DOI URL

指标 Index	LH	JS	FY	合计 Total
患病兔数Number of sick rabbits	41	38	33	112
大肠埃希菌分离数	29	26	23	78
Number of Escherichia coli infection
魏氏梭菌分离数	25	23	21	69
Number of Clostridium welchii infection
混合感染分离数	24	21	17	62
Number of mixed infection
球虫感染分离数	3	2	1	6
Number of coccidiosis infection
未定型杂菌分离数	2	1	1	4
Number of unformed bacteria infection

指标 Index	LH	JS	FY	合计 Total
患病兔数Number of sick rabbits	41	38	33	112
大肠埃希菌分离数	29	26	23	78
Number of Escherichia coli infection
魏氏梭菌分离数	25	23	21	69
Number of Clostridium welchii infection
混合感染分离数	24	21	17	62
Number of mixed infection
球虫感染分离数	3	2	1	6
Number of coccidiosis infection
未定型杂菌分离数	2	1	1	4
Number of unformed bacteria infection

试验地 Test site	组别 Group	预防数 Prevention number	发病数 Number of case	发病率 Incidence rate/%
LH	预防组Prevention group	45	6	13.3
	对照组Control group	45	29	64.4
JS	预防组Prevention group	45	4	8.9
	对照组Control group	45	27	60.0
FY	预防组Prevention group	45	2	4.4
	对照组Control group	45	25	55.6

试验地 Test site	组别 Group	预防数 Prevention number	发病数 Number of case	发病率 Incidence rate/%
LH	预防组Prevention group	45	6	13.3
	对照组Control group	45	29	64.4
JS	预防组Prevention group	45	4	8.9
	对照组Control group	45	27	60.0
FY	预防组Prevention group	45	2	4.4
	对照组Control group	45	25	55.6

试验地 Test site	组别 Group	患病数 Number of cases	病死数 Number of deaths	病死率 Mortality rate/%
LH	治疗组Treatment group	45	7	15.6
	对照组Control group	45	42	93.3
JS	预防组Prevention group	45	5	11.1
	对照组Control group	45	40	88.9
FY	预防组Prevention group	45	3	6.7
	对照组Control group	45	38	84.4

Isolation, identification and control of rabbit epidemic abdominal distension

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