Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2313-2319.DOI: 10.3969/j.issn.1004-1524.2021.12.11

• Horticultural Science • Previous Articles     Next Articles

Identification of quantitative trait loci controlling radish root index by QTL-seq

HU Tianhua(), WEI Qingzhen, WANG Jinglei, WANG Wuhong, HU Haijiao, YAN Yaqin, BAO Chonglai*()   

  1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  • Received:2020-06-09 Online:2021-12-25 Published:2022-01-10
  • Contact: BAO Chonglai

Abstract:

In order to detect QTL loci and find the key genes related to radish root shape index, an F2 population was constructed by crossing the small-sized oblate spheroidal radish LLYH and big-sized long white radish CLA which had significant differences in root shape index. QTL mapping for root shape index traits was conducted using QTL-Seq method. Four QTL loci were detected, of which two major QTL loci rs2.1 and rs2.2 were located in the 21.66-26.03 Mb and 30.79-36.56 Mb regions on chromosome R2, respectively; two minor QTL loci rs4.1 and rs6.1 were located on chromosome R4 39.04-40.43 Mb and R6 11.53-13.40 Mb.These results laid a foundation for the development of molecular markers linked to root shape index and provided an important reference for fine mapping the regulatory genes of the root shape index.

Key words: radish, root index, QTL-seq, QTL

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