Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)

doi:10.3969/j.issn.1004-1524.2021.12.14

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2339-2347.DOI: 10.3969/j.issn.1004-1524.2021.12.14

• Plant Protection • Previous Articles Next Articles

Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)

WANG Jun¹^,²(), WU Xiaobao¹^,², SONG Jia¹^,², DENG Qianqian¹^,², ZENG Guang¹^,², REN Mingjian³, YE Mao¹^,²^,^*()

1. Institute of Entomology, Guizhou University, Guiyang 550025, China
2. Guizhou Provincial Key Laboratory of Agricultural Pest Management of the Mountainous Regions, Guiyang 550025, China
3. Guizhou Branch of National Wheat Improvement Center, Guiyang 550025, China

Received:2021-03-10 Online:2021-12-25 Published:2022-01-10
Contact: YE Mao

Abstract

Abstract:

In order to study the interaction mediated by exogenous calcium between Triticum aestivum L. and Schizaphis graminum(Rondani), wheat seeds were soaked with calcium chloride solution (CaCl₂ treatment), while seeds soaked with distilled water were taken as control (CK). The changes of peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and β-1,3-glucanase (β-1,3-GA) activity in wheat leaves were detected at 0, 24, 48, 72 h after aphids infestation. Meanwhile, glutathione S-transferase (GSTs), carboxylesterase (CarE) and cytochrome P450 (CYP450) activities in aphids fed on wheat plant for 0, 24, 48, 72 h were tested as well. It was found that the aphids infestation significantly (P<0.05) induced POD, PAL and β-1,3-GA activities in wheat leaves, but PPO activity did not increase, while CaCl₂ treatment further significantly (P<0.05) enhanced activities of POD, PAL and β-1,3-GA. Besides,PPO activity in wheat plants was significantly (P<0.05) higher under CaCl₂ treatment than that under CK. Meanwhile, the activities of detoxification enzymes (GSTs, CarE and CYP450) in aphids fed on CaCl₂-treated plants were significantly (P<0.05) increased as compared with those fed on control plants. Collectively, the present results indicated that the exogenous calcium could induce the activities of defensive enzymes in wheat plants, while aphids increased their detoxification enzymes activities to adapt the enhanced defense of wheat.

Key words: wheat, Schizaphis graminum(Rondani), exogenous calcium, defense enzyme, detoxification enzyme

CLC Number:

S435
S512

WANG Jun, WU Xiaobao, SONG Jia, DENG Qianqian, ZENG Guang, REN Mingjian, YE Mao. Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2339-2347.

Figures/Tables 7

Fig. 1 Effect of different treatments on peroxidase (POD) activity of wheat leaves Bars marked without the same letters indicated significant(P<0.05) differences of enzymatic activity under the same treatment within different feeding time; “*”indicated significant (P<0.05) difference of enzymatic activity between treatments at the same feeding time.The same as below.

Fig.2 Effect of different treatments on polyphenol oxidase (PPO) activity of wheat leaves

Fig.3 Effect of different treatments on phenylalanine ammonia lyase (PAL) activity of wheat leaves

Fig.4 Effect of different treatments on β-1,3-glucanase (β-1,3-GA) activity of wheat leaves

Fig.5 Effect of different treatments on glutathione S-transferase (GSTs) activity of Schizaphis graminum(Rondani)

Fig.6 Effect of different treatments on carboxylesterase (CarE) activity of Schizaphis graminum(Rondani)

Fig.7 Effect of different treatments on cytochrome P450 activity of Schizaphis graminum(Rondani)

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Effect of seed soaking with exogenous calcium on activities of defense enzymes in wheat and detoxifying enzymes in Schizaphis graminum (Rondani)

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