Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

doi:10.3969/j.issn.1004-1524.2021.12.13

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2330-2338.DOI: 10.3969/j.issn.1004-1524.2021.12.13

• Horticultural Science • Previous Articles Next Articles

Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

CHEN Runchen(), WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei^*()

Yantai Institute of China Agricultural University, Yantai 264670, China

Received:2020-03-02 Online:2021-12-25 Published:2022-01-10
Contact: WANG Honglei

Abstract

Abstract:

In order to enrich the cultivated varieties of edible fungi, a wild fungus was identified by morphological and molecular biological methods, biological characteristics of hyphae were studied, artificial cultivation experiments were carried out, and nutritional components of fruiting bodies were determined. The results showed that the fungus was identified as Pholiota adiposa. The optimal growth temperature was 25 ℃, the optimal pH value was 5.0, and the optimal carbon source, nitrogen source and inorganic salt were sucrose, soybean cake powder and MnSO₄, respectively. Fruiting bodies were successfully cultivated from agricultural wastes such as sawdust and corncob, it took about 70 days from inoculation to harvest. Protein content, polysaccharide content, fat content and free amino acid content of fruit body were 19.94%, 5.14%, 2.32% and 2.84%, respectively.

Key words: Pholiota adiposa, biological characteristics, artificial cultivation, nutrient

CLC Number:

S646.1⁺9

CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei. Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2330-2338.

Figures/Tables 11

Fig. 1 Morphological observation of fruiting body A,Cap;B, Back of cap;C, Transverse section of the stalk; D, Veil and gils; E, Longitudinal section of fruiting body.

Fig.2 Basidiospores and spores print

Fig. 3 Morphology observation of colony and mycelium A,Colony; B, Hyphae and clamp connections(400×);C, Spores(400×).

Fig.4 Phylogenetic tree constructed from sequences of D1 and D2 region of 28S rDNA

Fig. 5 Mycelium productions of Pholiota adiposa EF-3 at different temperatures Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

Fig.6 Mycelium productions of Pholiota adiposa EF-3 at different pH values

Fig. 7 Mycelium productions of Pholiota adiposa EF-3 with different carbon sources A,Glucose; B,Sucrose; C,Starch; D,Corn powder; E,Brown sugar; F,Molasses; G,Cellulose; H,Maltose; I,Lactose.

Fig. 8 Mycelium productions of Pholiota adiposa EF-3 with different nitrogen sources A,Ammonium chloride; B,Ammonium sulfate; C,Ammonium nitrate; D,Potassium nitrate; E,Yeast extract powder; F,Potato powder; G,Beef extract powder; H,Peptone; I,Soybean cake powder; J,Tryptone; K,Soybean peptone.

Fig.9 Mycelium productions of Pholiota adiposa EF-3 with different inorganic salts

Fig.10 Cultivation process of Pholiota adiposa EF-3 A, Fruiting body appeared; B, Three days after fruiting body appeared; C, Five days after fruiting body appeared; D, Eight days after fruiting body appeared; E, Ten days after the fruiting body appeared; F, 12 days after the fruiting body appeared.

Fig.11 Nutrients and free amino acid contents of Pholiota adiposa EF-3fruitbody Asp,Aspartate; Glu,Glutamine; Ser,Serine; Thr,Threonine; Ala,Alanine; Tyr,Tyrosine; Ile,Isoleucine; Phe,Phenylalanine.

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Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

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