Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

doi:10.3969/j.issn.1004-1524.2021.12.13

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (12): 2330-2338.DOI: 10.3969/j.issn.1004-1524.2021.12.13

• Horticultural Science • Previous Articles Next Articles

Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

CHEN Runchen(), WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei^*()

Yantai Institute of China Agricultural University, Yantai 264670, China

Received:2020-03-02 Online:2021-12-25 Published:2022-01-10
Contact: WANG Honglei

Abstract

Abstract:

In order to enrich the cultivated varieties of edible fungi, a wild fungus was identified by morphological and molecular biological methods, biological characteristics of hyphae were studied, artificial cultivation experiments were carried out, and nutritional components of fruiting bodies were determined. The results showed that the fungus was identified as Pholiota adiposa. The optimal growth temperature was 25 ℃, the optimal pH value was 5.0, and the optimal carbon source, nitrogen source and inorganic salt were sucrose, soybean cake powder and MnSO₄, respectively. Fruiting bodies were successfully cultivated from agricultural wastes such as sawdust and corncob, it took about 70 days from inoculation to harvest. Protein content, polysaccharide content, fat content and free amino acid content of fruit body were 19.94%, 5.14%, 2.32% and 2.84%, respectively.

Key words: Pholiota adiposa, biological characteristics, artificial cultivation, nutrient

CLC Number:

S646.1⁺9

CHEN Runchen, WANG Yining, LIU Xiaowen, WANG Hongyan, DING Qiang, WANG Honglei. Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2330-2338.

Figures/Tables 11

Fig. 1 Morphological observation of fruiting body A,Cap;B, Back of cap;C, Transverse section of the stalk; D, Veil and gils; E, Longitudinal section of fruiting body.

Fig.2 Basidiospores and spores print

Fig. 3 Morphology observation of colony and mycelium A,Colony; B, Hyphae and clamp connections(400×);C, Spores(400×).

Fig.4 Phylogenetic tree constructed from sequences of D1 and D2 region of 28S rDNA

Fig. 5 Mycelium productions of Pholiota adiposa EF-3 at different temperatures Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

Fig.6 Mycelium productions of Pholiota adiposa EF-3 at different pH values

Fig. 7 Mycelium productions of Pholiota adiposa EF-3 with different carbon sources A,Glucose; B,Sucrose; C,Starch; D,Corn powder; E,Brown sugar; F,Molasses; G,Cellulose; H,Maltose; I,Lactose.

Fig. 8 Mycelium productions of Pholiota adiposa EF-3 with different nitrogen sources A,Ammonium chloride; B,Ammonium sulfate; C,Ammonium nitrate; D,Potassium nitrate; E,Yeast extract powder; F,Potato powder; G,Beef extract powder; H,Peptone; I,Soybean cake powder; J,Tryptone; K,Soybean peptone.

Fig.9 Mycelium productions of Pholiota adiposa EF-3 with different inorganic salts

Fig.10 Cultivation process of Pholiota adiposa EF-3 A, Fruiting body appeared; B, Three days after fruiting body appeared; C, Five days after fruiting body appeared; D, Eight days after fruiting body appeared; E, Ten days after the fruiting body appeared; F, 12 days after the fruiting body appeared.

Fig.11 Nutrients and free amino acid contents of Pholiota adiposa EF-3fruitbody Asp,Aspartate; Glu,Glutamine; Ser,Serine; Thr,Threonine; Ala,Alanine; Tyr,Tyrosine; Ile,Isoleucine; Phe,Phenylalanine.

References 28

[1]	李玉. 中国食用菌产业发展现状、机遇和挑战: 走中国特色菇业发展之路, 实现食用菌产业强国之梦[J]. 菌物研究, 2018, 16(3): 125-131.
	LI Y. The status, opportunities and challenges of edible fungi industry in China:develop with Chinese characteristics,realize the dream of powerful mushroom industrial country[J]. Journal of Fiungal Research, 2018, 16(3): 125-131.(in Chinese with English abstract)
[2]	李丽. 河南稳居全国食用菌头把交椅[N/OL]. 2019-06-06[2021-03-02]. https://newpaper.dahe.cn/dhbhtml2019-06/06/content_344606.htm.
[3]	张佰虎. 探究中国食用菌产业现状及未来发展[J]. 食品安全导刊, 2018(30): 67.
	ZHANG B H. Explore the current situation and future development of edible fungi industry in China[J]. China Food Safety Magazine, 2018(30): 67.(in Chinese)
[4]	李芬妮, 张俊飚, 姚方杰, 等. 中国食用菌产业技术对非合作与发展研究[J]. 中国工程科学, 2019, 21(4): 105-110.
	LI F N, ZHANG J B, YAO F J, et al. China-Africa cooperation in edible fungus industry technology[J]. Strategic Study of CAE, 2019, 21(4): 105-110.(in Chinese with English abstract)
[5]	田恩静, 图力古尔. 中国鳞伞属(广义)已知种类及其分布[J]. 菌物研究, 2004, 2(1): 25-34.
	TIAN E J, BAU T. Known species of Pholiota(s.l.) from China and their distribution[J]. Journal of Fungal Research, 2004, 2(1): 25-34.(in Chinese with English abstract)
[6]	中国食用菌协会. 2019年度全国食用菌统计调查结果分析[J]. 中国食用菌, 2021, 40(6): 104-110.
	China Edible Fungi Association. Analysis of statistical survey results of edible fungi in China in 2019[J]. Edible Fungi of China, 2021, 40(6): 104-110.(in Chinese)
[7]	戴玉成, 周丽伟, 杨祝良, 等. 中国食用菌名录[J]. 菌物学报, 2010, 29(1): 1-21.
	DAI Y C, ZHOU L W, YANG Z L, et al. A revised checklist of edible fungi in China[J]. Mycosystema, 2010, 29(1): 1-21.(in Chinese with English abstract)
[8]	李传华, 曲明清, 曹晖, 等. 中国食用菌普通名名录[J]. 食用菌学报, 2013, 20(3): 50-72.
	LI C H, QU M Q, CAO H, et al. Checklist of common names of mushrooms in China[J]. Acta Edulis Fungi, 2013, 20(3): 50-72.(in Chinese with English abstract)
[9]	孙丽君, 唐玉琴, 黄丹, 等. 金毛鳞伞液体培养基优化试验[J]. 湖北农业科学, 2013, 52(2): 378-380.
	SUN L J, TANG Y Q, HUANG D, et al. Experiment on optimizing the liquid medium for Pholiota attrivella[J]. Hubei Agricultural Sciences, 2013, 52(2): 378-380.(in Chinese with English abstract)
[10]	陈彦博. 黄伞的菌株鉴定及生物学特性研究[J]. 辽宁林业科技, 2018(3): 34-36.
	CHEN Y B. Strain identification and biological characteristic research of Pholiota adiposa[J]. Liaoning Forestry Science and Technology, 2018(3): 34-36.(in Chinese)
[11]	戴玉成, 杨祝良. 中国药用真菌名录及部分名称的修订[J]. 菌物学报, 2008, 27(6): 801-824.
	DAI Y C, YANG Z L. A revised checklist of medicinal fungi in China[J]. Mycosystema, 2008, 27(6): 801-824.(in Chinese with English abstract)
[12]	王晓岩, 图力古尔, 包海鹰. 多脂鳞伞研究进展[J]. 食用菌, 2018, 40(5): 1-3.
	WANG X Y, BAU T, BAO H Y. Research progress of Pholiota adiposa[J]. Edible Fungi, 2018, 40(5): 1-3.(in Chinese)
[13]	王慧超, 冉明才, 徐兴武, 等. 黄鳞伞栽培试验初报[J]. 南方农业, 2018, 12(30): 8-9.
	WANG H C, RAN M C, XU X W, et al. A preliminary report on the cultivation experiment of Pholiota adiposa[J]. South China Agriculture, 2018, 12(30): 8-9.(in Chinese)
[14]	张世卿. 食用菌基础[M]. 郑州: 中原农民出版社, 1997.
[15]	周德庆, 胡宝龙. 微生物学实验教程[M]. 2版. 北京: 高等教育出版社, 2006.
[16]	陈光富. 大型真菌担孢子形态观察常用方法简述[J]. 园艺与种苗, 2019, 39(3): 76-78.
	CHEN G F. A brief introduction to common methods for observation of morphology of macrofungi[J]. Horticulture & Seed, 2019, 39(3): 76-78.(in Chinese with English abstract)
[17]	应正河, 林衍铨, 江晓凌, 等. 绣球菌菌丝体及担孢子的电镜观察[J]. 福建农业学报, 2014, 29(2): 165-167.
	YING Z H, LIN Y Q, JIANG X L, et al. Observation in morphology of mycelium and basidiospore for Sparassis crispa with scanning electron microscope[J]. Fujian Journal of Agricultural Sciences, 2014, 29(2): 165-167.(in Chinese with English abstract)
[18]	王红艳, 孔维祎, 王竹青, 等. 一株野生香蘑属真菌的鉴定及其生物学性质[J]. 贵州农业科学, 2019, 47(6): 55-58.
	WANG H Y, KONG W Y, WANG Z Q, et al. Identification and biological characteristics of a wild mushroom Lepista sp[J]. Guizhou Agricultural Sciences, 2019, 47(6): 55-58.(in Chinese with English abstract)
[19]	李娟. 我国珍稀野生食用菌市场现状及营销探索[J]. 中国食用菌, 2019, 38(12): 105-107.
	LI J. Market status and marketing exploration of rare wild edible fungi in China[J]. Edible Fungi of China, 2019, 38(12): 105-107.(in Chinese with English abstract)
[20]	施水兵. 野生食用菌类的营养保健功能及食补运用[J]. 中国食品, 2018(24): 116.
	SHI S B. Food tonic application and health care function of wild edible fungi[J]. China Food, 2018(24): 116.(in Chinese)
[21]	秦淼. 野生食用菌资源利用现状及对策探究[J]. 南方农业, 2019, 13(Z1): 117-118.
	QIN M. Current situation and countermeasures of wild edible fungi resources utilization[J]. South China Agriculture, 2019, 13(Z1): 117-118.(in Chinese)
[22]	万涛. 野生食用菌资源利用现状及发展前景[J]. 河南农业, 2019(29): 41-42.
	WAN T. Utilization status and development prospect of wild edible fungi resources[J]. Henan Nongye, 2019(29): 41-42.(in Chinese)
[23]	图力古尔, 田恩静, 王欢. 中国的球盖菇科(一)鳞伞属[J]. 菌物研究, 2005, 3(3): 1-50.
	BAU T, TIAN E J, WANG H. Strophariaceae of China(1) Pholiota[J]. Journal of Fungal Research, 2005, 3(3): 1-50.(in Chinese with English abstract)
[24]	常骥, 曾宪录, 何显. 白鳞伞的特性及栽培驯化研究[J]. 食用菌, 2002, 24(4): 15-17.
	CHANG J, ZENG X L, HE X. Study on the characteristics, cultivation and domestication of Pholiota destruens[J]. Edible Fungi, 2002, 24(4): 15-17.(in Chinese)
[25]	吴金平, 付振艳, 王晓军, 等. 翘鳞伞驯化栽培研究[J]. 食用菌学报, 2011, 18(2): 24-25, 2.
	WU J P, FU Z Y, WANG X J, et al. Domestication and cultivation of Pholiota squarrosa[J]. Acta Edulis Fungi, 2011, 18(2): 24-25, 2.(in Chinese with English abstract)
[26]	田甜, 姚澜, 范冬雨, 等. 金针菇退化菌丝的生理生化特征[J]. 微生物学通报, 2021, 48(10): 3603-3611.
	TIAN T, YAO L, FAN D Y, et al. Physiological and biochemical characteristics of degenerate mycelium of Flammulina filiformis[J]. Microbiology China, 2021, 48(10): 3603-3611.(in Chinese with English abstract)
[27]	边银丙. 食用菌栽培学[M]. 3版. 北京: 高等教育出版社, 2017.
[28]	张静峰, 朱坚. 草菇厚垣孢子的研究[J]. 中国食用菌, 2004, 23(1): 13-15.
	ZHANG J F, ZHU J. Studies on chlamydospore of Volvariella volvacea[J]. Edible Fungi of China, 2004, 23(1): 13-15.(in Chinese with English abstract)

Identification, artificial cultivation and nutritional analysis of wild Pholiota adiposa

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