Screening of host factors interacting with wheat yellow mosaic virus P2 by yeast two-hybrid system

doi:10.3969/j.issn.1004-1524.2021.03.15

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 497-505.DOI: 10.3969/j.issn.1004-1524.2021.03.15

• Plant Protection • Previous Articles Next Articles

Screening of host factors interacting with wheat yellow mosaic virus P2 by yeast two-hybrid system

HAN Xiaolei¹^,²^,³, GAO Shiqi¹^,²^,³, ZHANG Fan³, YANG Jian³, LIU Peng³, JIANG Hongming¹, LI Linzhi¹^,^*()

1. Yantai Academy of Agricultural Sciences, Yantai 265500, China
2. College of Life Sciences, Yantai University, Yantai 265500, China
3. Ningbo University/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo 315000, China

Received:2020-10-27 Online:2021-04-02 Published:2021-03-25
Contact: LI Linzhi

Abstract

Abstract:

Wheat yellow mosaic virus (WYMV) is classified in the genus Bymovirus, family Potyviridae. The genome is composed of two sense RNA single-stranded RNA1 and RNA2 and encodes 10 proteins, among which P2 plays an important role in virus replication. In order to identify the targets of P2 in host, co-transformation of cDNA library vector and pGBKT7-P2 was constructed, eighteen candidate host targets of P2 were screened out, which were divided into several types based on their functions, including salicylic acid signal pathway factor, E3 ubiquitin ligase, defense response, oxidoreductase activity and photosystem II assembly. Additionally, the interaction of P2 and TaTIFY or Ta14-3-3 was further verified by Y2H system. Based on the results, it could be speculated that P2 may interact with E3 ubiquitin transcription factor, salicylic acid signal transcription factor, or the factors associated with plant photosystem and chloroplasts formation. In conclusion, P2 may participate in multiple signaling pathways during viral infection, and provide an insight into molecular interaction mechanism between WYMV and the host.

Key words: wheat yellow mosaic virus, wheat, P2, cDNA library, yeast two-hybrid

CLC Number:

S435.12

HAN Xiaolei, GAO Shiqi, ZHANG Fan, YANG Jian, LIU Peng, JIANG Hongming, LI Linzhi. Screening of host factors interacting with wheat yellow mosaic virus P2 by yeast two-hybrid system[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 497-505.

Figures/Tables 7

References 32

[1]	于嘉林, 晏立英, 苏宁 , 等. 小麦黄花叶病毒基因组核苷酸序列分析[J]. 中国科学C辑: 生命科学, 1999,29(6):639-644.
	YU J L, YAN L Y, SU N , et al. Genomic nucleotide sequence analysis of Wheat Yellow Mosaic Virus[J]. Science China(Series C), 1999,29(6):639-644.(in Chinese)
[2]	ABBINK T E M, PEART J R, MOS T N M , et al. Silencing of a gene encoding a protein component of the oxygen-evolving complex of photosystem II enhances virus replication in plants[J]. Virology, 2002,295(2):307-319. DOI URL PMID
[3]	DUNOYER P, THOMAS C, HARRISON S , et al. A cysteine-rich plant protein potentiates Potyvirus movement through an interaction with the virus genome-linked protein VPg[J]. Journal of Virology, 2004,78(5):2301-2309. DOI URL PMID
[4]	GOODFELLOW I, CHAUDHRY Y, GIOLDASI I , et al. Calicivirus translation initiation requires an interaction between VPg and eIF4E[J]. EMBO Reports, 2005,6(10):968-972. DOI URL PMID
[5]	PAUL A V, RIEDER E, KIM D W , et al. Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg[J]. Journal of Virology. 2000; 74(22):10359-10370. DOI URL PMID
[6]	KASHIWAZAKI S, MINOBE Y, OMURA T , et al. Nucleotide sequence of barley yellow mosaic virus RNA 1: a close evolutionary relationship with potyviruses[J]. The Journal of General Virology, 1990,71(Pt 12):2781-2790. DOI URL
[7]	CHEN J , SOHN, CHEN, et al. Molecular comparisons amongst wheat Bymovirus isolates from Asia, North America and Europe[J]. Plant Pathology, 1999,48(5):642-647. DOI URL
[8]	YU J L, YAN L Y, SU N , et al. Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs[J]. Science in China Series C: Life Sciences, 1999,42(5):554-560. DOI URL PMID
[9]	NAMBA S, KASHIWAZAKI S, LU X , et al. Complete nucleotide sequence of wheat yellow mosaic Bymovirus genomic RNAs[J]. Archives of Virology, 1998,143(4):631-643. DOI URL PMID
[10]	陈炯, 陈剑平 . 大麦黄花叶病毒属成员UTR系统进化树分析及编码蛋白跨膜结构推测[J]. 中国病毒学, 2002,17(4):49-54.
	CHEN J, CHEN J P . Phylogenetic tree analysis of UTR and transmembrane structure prediction of proteins of genus Bymovirus[J]. Virologica Sinica, 2002,17(4):49-54.(in Chinese with English abstract)
[11]	YOU Y, SHIRAKO Y . Bymovirus reverse genetics: requirements for RNA2-encoded proteins in systemic infection[J]. Molecular Plant Pathology, 2010,11(3):383-394. DOI URL PMID
[12]	LI H G, SHIRAKO Y . Association of VPg and eIF4E in the host tropism at the cellular level of Barley yellow mosaic virus and Wheat yellow mosaic virus in the genus Bymovirus[J]. Virology, 2015,476:159-167. DOI URL PMID
[13]	GARCÍA J A, PALLÁS V . Viral factors involved in plant pathogenesis[J]. Current Opinion in Virology, 2015,11:21-30. DOI URL PMID
[14]	COSTA M, NOBRE M S, BECKER J D , et al. Expression-based and co-localization detection of Arabinogalactan protein 6 and Arabinogalactan protein 11 interactors in Arabidopsis pollen and pollen tubes[J]. BMC Plant Biology, 2013,13:7. DOI URL PMID
[15]	SINGH R, LEE M O, LEE J E , et al. Rice mitogen-activated protein kinase interactome analysis using the yeast two-hybrid system[J]. Plant Physiology, 2012,160(1):477-487. DOI URL
[16]	ZHAO J P, LIU Q, ZHANG H L , et al. The rubisco small subunit is involved in Tobamovirus movement and Tm-22-mediated extreme resistance[J]. Plant Physiology, 2013,161(1):374-383. DOI URL
[17]	KONG L F, WU J X, LU L N , et al. Interaction between rice stripe virus disease-specific protein and host PsbP enhances virus symptoms[J]. Molecular Plant, 2014,7(4):691-708. DOI URL
[18]	SUN L Y, ANDIKA I B, SHEN J F , et al. The P2 of Wheat yellow mosaic virus rearranges the endoplasmic Reticulum and recruits other viral proteins into replication-associated inclusion bodies[J]. Molecular Plant Pathology, 2014,15(5):466-478. DOI URL
[19]	徐进, 李帅, 施季森 . 鹅掌楸属植物总RNA提取方法的比较与分析[J]. 福建林学院学报, 2008,28(2):156-159.
	XU J, LI S, SHI J S . Comparison and analysis of the methods of RNA isolation for Liriodendron genus[J]. Journal of Fujian College of Forestry, 2008,28(2):156-159.(in Chinese with English abstract)
[20]	GUTIERREZ C, RAMIREZ-PARRA E, MAR CASTELLANO M , et al. Geminivirus DNA replication and cell cycle interactions[J]. Veterinary Microbiology, 2004,98(2):111-119. DOI URL PMID
[21]	LI Y Z, CUI H G, CUI X Y , et al. The altered photosynthetic machinery during compatible virus infection[J]. Current Opinion in Virology, 2016,17:19-24. DOI URL PMID
[22]	ORUETXEBARRIA I, KVARNHEDEN A, VALKONEN J P T. Analysis of putative interactions between potyviral replication proteins and plant retinoblastoma proteins[J]. Virus Genes, 2002,24(1):65-75. DOI URL
[23]	KÜHNE T, SHI N N, PROESELER G , et al. The ability of a Bymovirus to overcome the rym4-mediated resistance in barley correlates with a Codon change in the VPg coding region on RNA1[J]. The Journal of General Virology, 2003,84(Pt 10):2853-2859. DOI URL PMID
[24]	BRACHMANN R K, BOEKE J D . Tag games in yeast: the two-hybrid system and beyond[J]. Current Opinion in Biotechnology, 1997,8(5):561-568. DOI URL PMID
[25]	KONG L J . A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants[J]. The EMBO Journal, 2000,19(13):3485-3495. DOI URL PMID
[26]	戚文平, 刘丽娟, 李宇 , 等. 用酵母双杂交筛选与小麦黄花叶病毒CP互作的寄主因子[J]. 麦类作物学报, 2016,36(4):415-419.
	QI W P, LIU L J, LI Y , et al. Screening of host factors interacting with wheat yellow mosaic virus CP by yeast two-hybrid system[J]. Journal of Triticeae Crops, 2016,36(4):415-419.(in Chinese with English abstract)
[27]	NA J K, KIM J K, KIM D Y , et al. Expression of potato RNA-binding proteins StUBA2a/b and StUBA2c induces hypersensitive-like cell death and early leaf senescence in Arabidopsis[J]. Journal of Experimental Botany, 2015,66(13):4023-4033. DOI URL PMID
[28]	LIU Q, ZHANG S H, LIU B . 14-3-3 proteins: Macro-regulators with great potential for improving abiotic stress tolerance in plants[J]. Biochemical and Biophysical Research Communications, 2016,477(1):9-13. DOI URL PMID
[29]	MENG L, ZHANG T, GENG S S , et al. Comparative proteomics and metabolomics of JAZ7-mediated drought tolerance in Arabidopsis[J]. Journal of Proteomics, 2019,196:81-91. DOI URL PMID
[30]	胡婷丽, 李魏, 刘雄伦 , 等. 泛素化在植物抗病中的作用[J]. 微生物学通报, 2014,41(6):1175-1179.
	HU T L, LI W, LIU X L , et al. The role of ubiquitination in plant disease resistance[J]. Microbiology China, 2014,41(6):1175-1179.(in Chinese with English abstract)
[31]	ZENG L R, VEGA-SÁNCHEZ M E, ZHU T, et al. Ubiquitination-mediated protein degradation and modification: an emerging theme in plant-microbe interactions[J]. Cell Research, 2006,16(5):413-426. DOI URL PMID
[32]	WANG Y S, PI L Y, CHEN X H , et al. Rice XA21 binding protein 3 is a ubiquitin ligase required for full Xa21-mediated disease resistance[J]. The Plant Cell, 2006,18(12):3635-3646. DOI URL PMID

编号 Number	引物名称 Primers name	引物序列 Primers sequence(5'-3')	酶切位点 RE site
1	pGADT7 forward primer	TAATACGACTCACTATAGGGCGA	SamⅠ
	pGADT7 reverse primer	CTGTGCATCGTGCACCATCT	BamHⅠ
2	pGBKT7 forward primer	GTAATACGACTCACTATAGGGCGA	NotⅠ
	pGBKT7 reverse primer	ATTCTCAGTGAAATTTTAAACATA	PstⅠ
3	Ta14-3-3 forward primer	TCCCCCGGGATGGCCTTCTTCTCCCACCAC	SamⅠ
	Ta14-3-3 reverse primer	CGCGGATCCTCACATATATATCTCCATGCC	BamHⅠ
4	WYMV-P2 forward primer	AAAGCGGCCGCATGTCAGCTGAGGAAACAGGT	NotⅠ
	WYMV-P2 reverse primer	GCACTGCAGATGGCCCGGCCTCCTGAGGAA	PstⅠ
5	TaTIFY 10A forward primer	TCCCCCGGGATGGCGGCTTCCGCGAGGCAG	SamⅠ
	TaTIFY 10A reverse primer	CGCGGATCCTTATTGGTTTGGCTTCAGGGC	BamHⅠ

编号 Number	引物名称 Primers name	引物序列 Primers sequence(5'-3')	酶切位点 RE site
1	pGADT7 forward primer	TAATACGACTCACTATAGGGCGA	SamⅠ
	pGADT7 reverse primer	CTGTGCATCGTGCACCATCT	BamHⅠ
2	pGBKT7 forward primer	GTAATACGACTCACTATAGGGCGA	NotⅠ
	pGBKT7 reverse primer	ATTCTCAGTGAAATTTTAAACATA	PstⅠ
3	Ta14-3-3 forward primer	TCCCCCGGGATGGCCTTCTTCTCCCACCAC	SamⅠ
	Ta14-3-3 reverse primer	CGCGGATCCTCACATATATATCTCCATGCC	BamHⅠ
4	WYMV-P2 forward primer	AAAGCGGCCGCATGTCAGCTGAGGAAACAGGT	NotⅠ
	WYMV-P2 reverse primer	GCACTGCAGATGGCCCGGCCTCCTGAGGAA	PstⅠ
5	TaTIFY 10A forward primer	TCCCCCGGGATGGCGGCTTCCGCGAGGCAG	SamⅠ
	TaTIFY 10A reverse primer	CGCGGATCCTTATTGGTTTGGCTTCAGGGC	BamHⅠ

编号 Number	序列名 Sequence ID	基因名 Gene name	功能 Function
1	TraesCS2A02G407700.1	TapTOM75	Aquaporin PIP-type pTOM75
2	TraesCS5A02G322500.1	TaCB36	Chlorophyll a-b binding protein 36
3	TraesCS6A02G245100.1	TaRS31	Serine/arginine-rich splicing factor RS31-like
4	TraesCS2D02G248400.2	TaOEE1	Oxygen-evolving enhancer protein 1
5	TraesCS5A02G164600.1	TaLIP	Light-induced protein
6	TraesCS3D02G055500.1	Ta14-3-3	14-3-3-like protein C
7	TraesCS2B02G195600.1	TaTIFY 10A	Protein TIFY 10A-like
8	TraesCS3D02G247300.2	TaID31	Protein IQ-DOMAIN 31-like
9	TraesCS7A02G235200.3	TaAGWD	Alpha-glucan water dikinase
10	TraesCS7D02G276300.1	TaCBP21	Chlorophyll a-b binding protein 21
11	TraesCS3D02G232300.1	TaUBA2c	UBP1-associated protein 2C-like
12	TraesCS2B02G126500.1	TaRL19-3	60S ribosomal protein L19-3-like
13	TraesCS3D02G079600.2	TaBAG6	Large proline-rich protein BAG6
14	TraesCS4A02G159700.2	TaCGS1	Cystathionine gamma-synthase 1
15	TraesCS2D02G253900.1	TaPSP	Photosystem II 10 kDa polypeptide
16	TraesCS3A02G309800.1	TaCNR8	Cell number regulator 8-like
17	TraesCS5D02G169600.2	TaRBCSC	Ribulose bisphosphate carboxylase small chain
18	TraesCS4D02G215600.2	TaBOI	BOI-related E3 ubiquitin-protein ligase 3

编号 Number	序列名 Sequence ID	基因名 Gene name	功能 Function
1	TraesCS2A02G407700.1	TapTOM75	Aquaporin PIP-type pTOM75
2	TraesCS5A02G322500.1	TaCB36	Chlorophyll a-b binding protein 36
3	TraesCS6A02G245100.1	TaRS31	Serine/arginine-rich splicing factor RS31-like
4	TraesCS2D02G248400.2	TaOEE1	Oxygen-evolving enhancer protein 1
5	TraesCS5A02G164600.1	TaLIP	Light-induced protein
6	TraesCS3D02G055500.1	Ta14-3-3	14-3-3-like protein C
7	TraesCS2B02G195600.1	TaTIFY 10A	Protein TIFY 10A-like
8	TraesCS3D02G247300.2	TaID31	Protein IQ-DOMAIN 31-like
9	TraesCS7A02G235200.3	TaAGWD	Alpha-glucan water dikinase
10	TraesCS7D02G276300.1	TaCBP21	Chlorophyll a-b binding protein 21
11	TraesCS3D02G232300.1	TaUBA2c	UBP1-associated protein 2C-like
12	TraesCS2B02G126500.1	TaRL19-3	60S ribosomal protein L19-3-like
13	TraesCS3D02G079600.2	TaBAG6	Large proline-rich protein BAG6
14	TraesCS4A02G159700.2	TaCGS1	Cystathionine gamma-synthase 1
15	TraesCS2D02G253900.1	TaPSP	Photosystem II 10 kDa polypeptide
16	TraesCS3A02G309800.1	TaCNR8	Cell number regulator 8-like
17	TraesCS5D02G169600.2	TaRBCSC	Ribulose bisphosphate carboxylase small chain
18	TraesCS4D02G215600.2	TaBOI	BOI-related E3 ubiquitin-protein ligase 3

Screening of host factors interacting with wheat yellow mosaic virus P2 by yeast two-hybrid system

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