Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

doi:10.3969/j.issn.1004-1524.2021.03.16

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 506-515.DOI: 10.3969/j.issn.1004-1524.2021.03.16

• Plant Protection • Previous Articles Next Articles

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

FAN Linjuan¹, LIU Zirong¹, XU Xueliang¹, WANG Fenshan¹, PENG Deliang², YAO Yingjuan¹^,^*()

1. Institute of Applied Agricultural Micro-Organisms, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

Received:2020-08-25 Online:2021-03-25 Published:2021-03-25
Contact: YAO Yingjuan

Abstract

Abstract:

To clarify the effect of nematicides on soil environment, the effects of two kinds of chemical nematicides (avifosthiazate granules and fluopyram) and four kinds of biological nematicides (Verticillium chlamydosporium, Paecilomyces lilacinus, horseradish and tea curd) on soil microbe quantity, enzymes activities and soil nutrients in the replanted Chinese yam field at seedling stage and maturity stage were studied by conventional analysis methods. The results indicated that, compared with the control without nematicides, two kinds of chemical nematicides significantly (P<0.05) increased the number of soil bacteria and fungi at seedling stage, but the number of soil bacteria and fungi all decreased to the same level as control or lower at maturity stage. Among the four kinds of biological nematicides, tea curd significantly (P<0.05) inhibited the ratio of bacteria to fungi (bacteria/fungi), yet significantly (P<0.05) increased the number of fungi at seedling stage, and significantly (P<0.05) increased the number of soil bacteria and bacteria/fungi at maturity stage. All the treatments had no significant effect on soil urease activity. Two kinds of chemical nematicides and V. chlamydosporium, P. lilacinus, horseradish had significant (P<0.05) promoting and inhibiting effects on soil acid phosphatase activity at seedling stage and maturity stage, respectively; while the activity of acid phosphatase was significantly (P<0.05) inhibited by tea curd at maturity stage. The activity of soil sucrase at seedling stage and maturity stage was significantly (P<0.05) inhibited and promoted by the tea curd, respectively, while all the other nematicides showed significant (P<0.05) inhibition effect all the time. The contents of soil ammonium nitrogen, available potassium and available phosphorus were significantly (P<0.05) affected by nematicides. The contents of soil ammonium nitrogen and available potassium had no significant change compared with that of control at seedling stage after application of nematicides. At maturity stage, the contents of ammonium nitrogen and available potassium in other treatments were significantly (P<0.05) higher than those in the control except for the content of ammonium nitrogen with application of tea curd. At seedling stage, the content of soil available phosphorus with application of nematicides was significantly (P<0.05) lower than that of control, but at maturity stage, there was no significant difference in the content of soil available phosphorus. In conclusion, the effects of these nematicides on soil microbial population, soil urease activity and soil nutrients could be gradually recovered with the prolonged time, and showed no potential harm to soil fertility and crop growth, while the effects on soil acid phosphatase activity and sucrase activity might interfere with soil organic phosphorus mineralization and carbon cycle to some extent.

Key words: Chinese yam, nematicides, enzyme activity, soil nutrients

CLC Number:

S154

FAN Linjuan, LIU Zirong, XU Xueliang, WANG Fenshan, PENG Deliang, YAO Yingjuan. Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 506-515.

Figures/Tables 7

References 34

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处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.77±0.17 ab	0.10±0.01 a	5.67±0.48 e	41.45±2.51 e	ND
T1	5.27±0.02 a	0.13±0.08 a	42.37±1.59 b	74.50±1.33 c	1.00±1.00 a
T2	4.66±0.18 b	0.30±0.06 a	35.57±1.21 c	55.73±4.91 d	0.83±0.33 a
T3	4.71±0.09 b	0.20±0.10 a	15.17±1.13 d	74.03±6.11 c	0.47±0.47 a
T4	4.98±0.21 ab	0.17±0.12 a	14.00±1.79 d	89.55±1.36 b	0.33±0.17 a
T5	4.44±0.25 b	0.13±0.07 a	72.83±4.37 a	108.00±4.62 a	1.73±0.72 a
T6	4.68±0.15 b	0.18±0.02 a	11.33±0.67 de	99.33±0.67 ab	ND

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

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