Effect of biochar on wheat root-associated microbial community structures

doi:10.3969/j.issn.1004-1524.2021.03.17

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 516-525.DOI: 10.3969/j.issn.1004-1524.2021.03.17

• Plant Protection • Previous Articles Next Articles

Effect of biochar on wheat root-associated microbial community structures

XU Minmin¹, HUANG Ying¹, LI Bo¹, XU Yan², ZHANG Shuai², YAO Lingyun², WANG Zheng²

1. Shandong Academy of Environmental Sciences Co., Ltd., Jinan 250100, China
2. College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China

Received:2020-08-17 Online:2021-03-25 Published:2021-03-25

Abstract

Abstract:

In order to study the effect of biochar on the wheat root-associated microbiome, greenhouse experiment was conducted and the microbial communities in wheat endosphere, rhizosphere and bulk soil were sequenced by high throughput sequencing technology. The results showed that the microbial community diversity in endosphere was significantly (P<0.05) lower than that in the rhizosphere and bulk soil. In other words, plant roots could filter and screen some microorganisms to colonize and grow in the roots, among which Proteobacteria and Cyanobacteria were the dominant bacteria. Biochar could influence the structure and composition of wheat root-associated microbial community. In the endosphere, the relative abundance of Verrucomicrobiaceae and Luteolibacter was significantly (P<0.05) increased with biochar addition; while in the rhizosphere, biochar addition significantly (P<0.05) increased the relative abundance of Verrucomicrobia, Firmicutes, Gemmatimonadetes, Bacillales, Alicyclobacillaceae, Luteolibacter, Tumebacillus, Gemmatimonas, Kosakonia, Lysobacter, Pseudoxanthomonas, Blastomonas, Mssilia, Archangium and Enterobacter. The microbial communities in the rhizosphere was more sensitive to biochar, and the addition of biochar significantly (P<0.05) changed the biomarkers in the rhizosphere of wheat.

Key words: wheat, biochar, high throughput sequencing, microbial diversity

CLC Number:

S154.3

XU Minmin, HUANG Ying, LI Bo, XU Yan, ZHANG Shuai, YAO Lingyun, WANG Zheng. Effect of biochar on wheat root-associated microbial community structures[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 516-525.

Figures/Tables 6

Fig.1 Venn diagram of microbial community composition under different treatments E, Microbiome in endosphere without biochar addition; R, Microbiome in rhizosphere without biochar addition; S, Microbiome in bulk soils without biochar addition; EB, Microbiome in endosphere with biochar addition; RB, Microbiome in rhizosphere with biochar addition; SB, Microbiome in bulk soils with biochar addition. The same as below. a, Comparison of microbiome without biochar addition; b, Comparison of microbiome with biochar addition; c, Comparison of microbiome in endosphere under different treatments; d, Comparison of microbiome in rhizosphere under different treatments; e, Comparison of microbiome in bulk soils under different treatments.

Fig.2 Shannon index under different treatments Bars marked without the same letters indicated significant difference at P<0.05.

Fig.3 PCoA analysis of microbial community in endosphere, rhizosphere and bunk soil under all treatments

Fig.4 Relative abundance of main phyla under treatments without (a) or with (b) biochar addition

Fig.5 Analysis of main microbial community composition at genus level The relative abundance was calculated by common logarithm.

Fig.6 Comparison of microbial community in endosphere (a) and rhizosphere (b) under treatments with or without biochar addition LDA score was the result after common logarithm.

References 33

[1]	方婧, 金亮, 程磊磊 , 等. 环境中生物质炭稳定性研究进展[J]. 土壤学报, 2019,56(5):1034-1047.
	FANG J, JIN L, CHENG L L , et al. Advancement in research on stability of biochar in the environment[J]. Acta Pedologica Sinica, 2019,56(5):1034-1047.(in Chinese with English abstract)
[2]	姜玉萍, 杨晓峰, 张兆辉 , 等. 生物炭对土壤环境及作物生长影响的研究进展[J]. 浙江农业学报, 2013,25(2):410-415.
	JIANG Y P, YANG X F, ZHANG Z H , et al. Progress of the effect of biomass charcoal on soil environment and crop growth[J]. Acta Agriculturae Zhejiangensis, 2013,25(2):410-415.(in Chinese with English abstract)
[3]	刘园, KHAN M J, 靳海洋, 等. 秸秆生物炭对潮土作物产量和土壤性状的影响[J]. 土壤学报, 2015,52(4):849-858.
	LIU Y, KHAN M J, JIN H Y , et al. Effects of successive application of crop-straw biochar on crop yield and soil properties in cambosols[J]. Acta Pedologica Sinica, 2015,52(4):849-858.(in Chinese with English abstract)
[4]	雷海迪, 尹云锋, 刘岩 , 等. 杉木凋落物及其生物炭对土壤微生物群落结构的影响[J]. 土壤学报, 2016,53(3):790-799.
	LEI H D, YIN Y F, LIU Y , et al. Effects of fir (Cunninghamia lanceolata) litter and its biochar on soil microbial community structure[J]. Acta Pedologica Sinica, 2016,53(3):790-799.(in Chinese with English abstract)
[5]	吴林坤, 林向民, 林文雄 . 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J]. 植物生态学报, 2014,38(3):298-310.
	WU L K, LIN X M, LIN W X . Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates[J]. Chinese Journal of Plant Ecology, 2014,38(3):298-310.(in Chinese with English abstract)
[6]	BERENDSEN R L, PIETERSE C M J, BAKKER P A H M. The rhizosphere microbiome and plant health[J]. Trends in Plant Science, 2012,17(8):478-486. DOI URL PMID
[7]	PHILIPPOT L, RAAIJMAKERS J M, LEMANCEAU P , et al. Going back to the roots: the microbial ecology of the rhizosphere[J]. Nature Reviews Microbiology, 2013,11(11):789-799. DOI URL PMID
[8]	MENDES R, KRUIJT M, DE BRUIJN I , et al. Deciphering the rhizosphere microbiome for disease-suppressive bacteria[J]. Science, 2011,332(6033):1097-1100. DOI URL PMID
[9]	ALI B, SABRI A N, LJUNG K , et al. Auxin production by plant associated bacteria: impact on endogenous IAA content and growth of Triticum aestivum L[J]. Letters in Applied Microbiology, 2009,48(5):542-547. DOI URL PMID
[10]	LUNDBERG D S, LEBEIS S L, PAREDES S H , et al. Defining the core Arabidopsis thaliana root microbiome[J]. Nature, 2012,488(7409):86-90. DOI URL PMID
[11]	BULGARELLI D, ROTT M, SCHLAEPPI K , et al. Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J]. Nature, 2012,488(7409):91-95. DOI URL PMID
[12]	张又弛, 李会丹 . 生物炭对土壤中微生物群落结构及其生物地球化学功能的影响[J]. 生态环境学报, 2015,24(5):898-905.
	ZHANG Y C, LI H D . Influence of biochar on the community structure and biogeochemical functions of microorganisms in soils[J]. Ecology and Environmental Sciences, 2015,24(5):898-905.(in Chinese with English abstract)
[13]	GRABER E R, MELLER HAREL Y, KOLTON M , et al. Biochar impact on development and productivity of pepper and tomato grown in fertigated soilless media[J]. Plant and Soil, 2010,337(1/2):481-496.
[14]	邵慧芸, 张阿凤, 王旭东 , 等. 两种生物炭对烤烟生长、根际土壤性质和微生物群落结构的影响[J]. 环境科学学报, 2019,39(2):537-544.
	SHAO H Y, ZHANG A F, WANG X D , et al. Effects of two kinds of biochar on the flue-cured tobacco growth, soil properties and microbial community structure of rhizosphere soil[J]. Acta Scientiae Circumstantiae, 2019,39(2):537-544.(in Chinese with English abstract)
[15]	顾美英, 徐万里, 唐光木 , 等. 生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响[J]. 新疆农业科学, 2014,51(5):926-934.
	GU M Y, XU W L, TANG G M , et al. Effects of biochar on soil microbial diversity and function related with N transformation in grey desert soil and aeolian sandy soil in Xinjiang[J]. Xinjiang Agricultural Sciences, 2014,51(5):926-934.(in Chinese with English abstract)
[16]	MENG J, ZHONG L B, WANG L , et al. Contrasting effects of alkaline amendments on the bioavailability and uptake of Cd in rice plants in a Cd-contaminated acid paddy soil[J]. Environmental Science and Pollution Research, 2018,25(9):8827-8835. DOI URL PMID
[17]	EDWARDS J, JOHNSON C, SANTOS-MEDELLÍN C, et al. Structure, variation, and assembly of the root-associated microbiomes of rice[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015,112(8):E911-E920. URL PMID
[18]	XU Y, GE Y, SONG J X , et al. Assembly of root-associated microbial community of typical rice cultivars in different soil types[J]. Biology and Fertility of Soils, 2020,56(2):249-260.
[19]	HUGERTH L W, WEFER H A, LUNDIN S , et al. DegePrime, a program for degenerate primer design for broad-taxonomic-range PCR in microbial ecology studies[J]. Applied and Environmental Microbiology, 2014,80(16):5116-5123. DOI URL PMID
[20]	COLEMAN-DERR D, DESGARENNES D, FONSECA-GARCIA C , et al. Plant compartment and biogeography affect microbiome composition in cultivated and native Agave species[J]. New Phytologist, 2016,209(2):798-811.
[21]	程扬, 刘子丹, 沈启斌 , 等. 秸秆生物炭施用对玉米根际和非根际土壤微生物群落结构的影响[J]. 生态环境学报, 2018,27(10):1870-1877.
	CHENG Y, LIU Z D, SHEN Q B , et al. The impact of straw biochar on corn rhizospheric and non-rhizospheric soil microbial community structure[J]. Ecology and Environmental Sciences, 2018,27(10):1870-1877.(in Chinese with English abstract)
[22]	邹春娇, 张勇勇, 张一鸣 , 等. 生物炭对设施连作黄瓜根域基质酶活性和微生物的调节[J]. 应用生态学报, 2015,26(6):1772-1778.
	ZOU C J, ZHANG Y Y, ZHANG Y M , et al. Regulation of biochar on matrix enzyme activities and microorganisms around cucumber roots under continuous cropping[J]. Chinese Journal of Applied Ecology, 2015,26(6):1772-1778.(in Chinese with English abstract)
[23]	VANDENKOORNHUYSE P, QUAISER A, DUHAMEL M , et al. The importance of the microbiome of the plant holobiont[J]. New Phytologist, 2015,206(4):1196-1206.
[24]	BULGARELLI D, ROTT M, SCHLAEPPI K , et al. Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J]. Nature, 2012,488(7409):91-95. DOI URL PMID
[25]	HACQUARD S . Disentangling the factors shaping microbiota composition across the plant holobiont[J]. New Phytologist, 2016,209(2):454-457. DOI URL
[26]	任慧爽, 徐伟芳, 王爱印 , 等. 桑树内生细菌多样性及内生拮抗活性菌群的研究[J]. 西南大学学报(自然科学版), 2017,39(1):36-45.
	REN H S, XU W F, WANG A Y , et al. Research on biodiversity of endophytic bacteria and the antagonistic endophytes in mulberry[J]. Journal of Southwest University (Natural Science Edition), 2017,39(1):36-45.(in Chinese with English abstract)
[27]	OFEK M, HADAR Y, MINZ D . Ecology of root colonizing Massilia(Oxalobacteraceae)[J]. PLoS One, 2012,7(7):e40117. DOI URL PMID
[28]	CHUNG E J, JO E J, YOON H S , et al. Sphingomonas oryziterrae sp. nov. and Sphingomonas jinjuensis sp. nov. isolated from rhizosphere soil of rice (Oryza sativa L.)[J]. International Journal of Systematic and Evolutionary Microbiology, 2011,61(10):2389-2394. DOI URL
[29]	NIELSEN S, MINCHIN T, KIMBER S , et al. Comparative analysis of the microbial communities in agricultural soil amended with enhanced biochars or traditional fertilisers[J]. Agriculture, Ecosystems & Environment, 2014,191:73-82.
[30]	韩光明, 孟军, 曹婷 , 等. 生物炭对菠菜根际微生物及土壤理化性质的影响[J]. 沈阳农业大学学报, 2012,43(5):515-520.
	HAN G M, MENG J, CAO T , et al. Effect of biochar on microorganisms quantities and soil physicochemical property in rhizosphere of spinach[J]. Journal of Shenyang Agricultural University, 2012,43(5):515-520.(in Chinese with English abstract)
[31]	王明元, 侯式贞, 董涛 , 等. 香蕉假茎生物炭对根际土壤细菌丰度和群落结构的影响[J]. 微生物学报, 2019,59(7):1363-1372. DOI URL
	WANG M Y, HOU S Z, DONG T , et al. Effects of banana pseudostem biochar on bacterial abundance and community structure in rhizosphere soil[J]. Acta Microbiologica Sinica, 2019,59(7):1363-1372.(in Chinese with English abstract) DOI URL
[32]	PRAMANIK K, MITRA S, SARKAR A , et al. Alleviation of phytotoxic effects of cadmium on rice seedlings by cadmium resistant PGPR strain Enterobacter aerogenes MCC 3092[J]. Journal of Hazardous Materials, 2018,351:317-329. DOI URL PMID
[33]	KILIC-EKICI O, YUEN G Y . Comparison of strains of Lysobacter enzymogenes and PGPR for induction of resistance against Bipolaris sorokiniana in tall fescue[J]. Biological Control, 2004,30(2):446-455. DOI URL

Effect of biochar on wheat root-associated microbial community structures

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