Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

doi:10.3969/j.issn.1004-1524.2021.03.16

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (3): 506-515.DOI: 10.3969/j.issn.1004-1524.2021.03.16

• Plant Protection • Previous Articles Next Articles

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

FAN Linjuan¹, LIU Zirong¹, XU Xueliang¹, WANG Fenshan¹, PENG Deliang², YAO Yingjuan¹^,^*()

1. Institute of Applied Agricultural Micro-Organisms, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

Received:2020-08-25 Online:2021-04-02 Published:2021-03-25
Contact: YAO Yingjuan

Abstract

Abstract:

To clarify the effect of nematicides on soil environment, the effects of two kinds of chemical nematicides (avifosthiazate granules and fluopyram) and four kinds of biological nematicides (Verticillium chlamydosporium, Paecilomyces lilacinus, horseradish and tea curd) on soil microbe quantity, enzymes activities and soil nutrients in the replanted Chinese yam field at seedling stage and maturity stage were studied by conventional analysis methods. The results indicated that, compared with the control without nematicides, two kinds of chemical nematicides significantly (P<0.05) increased the number of soil bacteria and fungi at seedling stage, but the number of soil bacteria and fungi all decreased to the same level as control or lower at maturity stage. Among the four kinds of biological nematicides, tea curd significantly (P<0.05) inhibited the ratio of bacteria to fungi (bacteria/fungi), yet significantly (P<0.05) increased the number of fungi at seedling stage, and significantly (P<0.05) increased the number of soil bacteria and bacteria/fungi at maturity stage. All the treatments had no significant effect on soil urease activity. Two kinds of chemical nematicides and V. chlamydosporium, P. lilacinus, horseradish had significant (P<0.05) promoting and inhibiting effects on soil acid phosphatase activity at seedling stage and maturity stage, respectively; while the activity of acid phosphatase was significantly (P<0.05) inhibited by tea curd at maturity stage. The activity of soil sucrase at seedling stage and maturity stage was significantly (P<0.05) inhibited and promoted by the tea curd, respectively, while all the other nematicides showed significant (P<0.05) inhibition effect all the time. The contents of soil ammonium nitrogen, available potassium and available phosphorus were significantly (P<0.05) affected by nematicides. The contents of soil ammonium nitrogen and available potassium had no significant change compared with that of control at seedling stage after application of nematicides. At maturity stage, the contents of ammonium nitrogen and available potassium in other treatments were significantly (P<0.05) higher than those in the control except for the content of ammonium nitrogen with application of tea curd. At seedling stage, the content of soil available phosphorus with application of nematicides was significantly (P<0.05) lower than that of control, but at maturity stage, there was no significant difference in the content of soil available phosphorus. In conclusion, the effects of these nematicides on soil microbial population, soil urease activity and soil nutrients could be gradually recovered with the prolonged time, and showed no potential harm to soil fertility and crop growth, while the effects on soil acid phosphatase activity and sucrase activity might interfere with soil organic phosphorus mineralization and carbon cycle to some extent.

Key words: Chinese yam, nematicides, enzyme activity, soil nutrients

CLC Number:

S154

FAN Linjuan, LIU Zirong, XU Xueliang, WANG Fenshan, PENG Deliang, YAO Yingjuan. Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 506-515.

Figures/Tables 7

References 34

[1]	涂伟凤, 汤洁, 涂玉琴 , 等. 江西山药生产现状及发展优势[J]. 江西农业学报, 2012,24(11):21-24.
	TU W F, TANG J, TU Y Q , et al. Advantage and status of Chinese yam production in Jiangxi[J]. Acta Agriculturae Jiangxi, 2012,24(11):21-24. (in Chinese with English abstract)
[2]	刘廷辉, 贾海民, 李瑞军 , 等. 6种药剂对山药种薯短体线虫的防治效果[J]. 农药, 2017,56(6):450-452.
	LIU T H, JIA H M, LI R J , et al. Control effect of six kinds of insecticides against Pratylenchus on the yam seed[J]. Agrochemicals, 2017,56(6):450-452.(in Chinese with English abstract)
[3]	贺哲, 黄婷, 李俊科 , 等. 瑞昌山药根腐线虫病病原鉴定[J]. 江西农业大学学报, 2016,38(5):879-883.
	HE Z, HUANG T, LI J K , et al. Identification of nematode causing yam root rot in Ruichang City, Jiangxi Province[J]. Acta Agriculturae Universitatis Jiangxiensis, 2016,38(5):879-883.(in Chinese with English abstract)
[4]	谢平 . 生物肥料“宁盾”对药用植物根结线虫病防治及促生研究[D]. 南京: 南京农业大学, 2015.
	XIE P . Research on promotion and biocontrol of root-knot disease on medicinal plants by bio-fertilizer “NS”[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese with English abstract)
[5]	石鸿文, 谢风超 . 山药根结线虫病综合防治技术[J]. 河南农业科学, 2003,32(3):42.
	SHI H W, XIE F C . Integrated control technology of root-knot nematode in Chinese yam[J]. Journal of Henan Agricultural Sciences, 2003,32(3):42.(in Chinese)
[6]	CLAUDIUS-COLE A O, FAWOLE B, ASIEDU R , et al. Management of Meloidogyne incognita in yam-based cropping systems with cover crops[J]. Crop Protection, 2014,63:97-102. DOI URL
[7]	CLAUDIUS-COLE A O, OMOTAYO T O, MONTES A L . Nodal vine cutting technique for assessing nematode resistance in yams[J]. Tropical Plant Pathology, 2020,45(1):56-63. DOI URL
[8]	黄婷, 蒋军喜, 余国庆 , 等. 山药病害最新研究进展[J]. 生物灾害科学, 2014,37(1):74-78.
	HUANG T, JIANG J X, YU G Q , et al. Latest advance in researches on Dioscorea opposite thunb diseases[J]. Biological Disaster Science, 2014,37(1):74-78.(in Chinese with English abstract)
[9]	迟元凯, 叶梦迪, 赵伟 , 等. 氟吡菌酰胺对南方根结线虫的作用效果[J]. 植物保护学报, 2019,46(6):1364-1370.
	CHI Y K, YE M D, ZHAO W , et al. Effect of fluopyram on root-knot nematode Meloidogyne incognita[J]. Journal of Plant Protection, 2019,46(6):1364-1370.(in Chinese with English abstract)
[10]	董文芳, 刘廷辉, 贾海民 , 等. 3种药剂对山药短体线虫病的田间防治效果[J]. 河北农业科学, 2017,21(1):46-48.
	DONG W F, LIU T H, JIA H M , et al. Field efficacy of three nematocides on the shortbody nematode of Dioscorea opposite thunb[J]. Journal of Hebei Agricultural Sciences, 2017,21(1):46-48. (in Chinese with English abstract)
[11]	FASKE T R, HURD K . Sensitivity of Meloidogyne incognita and Rotylenchulus reniformis to fluopyram[J]. Journal of Nematology, 2015,47(4):316-321. URL PMID
[12]	时立波, 王振华, 刘静 , 等. 几种杀线剂对土壤微生物数量及真菌多样性影响[J]. 农药, 2008,47(12):917-919.
	SHI L B, WANG Z H, LIU J , et al. Effects of different nematicides on number of soil microorganism and fungi diversity[J]. Agrochemicals, 2008,47(12):917-919.(in Chinese with English abstract)
[13]	杨叶青, 范琳娟, 刘奇志 , 等. 棉隆和氯化苦熏蒸对重茬草莓土壤线虫群落及养分含量的影响[J]. 园艺学报, 2018,45(4):725-733.
	YANG Y Q, FAN L J, LIU Q Z , et al. Effects of dazomet and chloropicrin on the soil nematode communities and nutrient content of replanted strawberry[J]. Acta Horticulturae Sinica, 2018,45(4):725-733.(in Chinese with English abstract)
[14]	刘淑琮, 冯炘, 于洁 . 植物根际促生菌的研究进展及其环境作用[J]. 湖北农业科学, 2009,48(11):2882-2887.
	LIU S C, FENG X, YU J . Research progress of plant growth-promoting rhizobacteria and its environmental effects[J]. Hubei Agricultural Sciences, 2009,48(11):2882-2887.(in Chinese with English abstract)
[15]	张焕军, 郁红艳, 丁维新 . 长期施用有机无机肥对潮土微生物群落的影响[J]. 生态学报, 2011,31(12):3308-3314.
	ZHANG H J, YU H Y, DING W X . The influence of the long-term application of organic manure and mineral fertilizer on microbial community in calcareous fluvo-aquic soil[J]. Acta Ecologica Sinica, 2011,31(12):3308-3314.(in Chinese with English abstract)
[16]	ELLIS R J, NEISH B, TRETT M W , et al. Comparison of microbial and meiofaunal community analyses for determining impact of heavy metal contamination[J]. Journal of Microbiological Methods, 2001,45(3):171-185. DOI URL PMID
[17]	邹小明, 朱立成, 肖春玲 , 等. 三唑磷的土壤微生物生态效应研究[J]. 农业环境科学学报, 2008,27(1):238-242.
	ZOU X M, ZHU L C, XIAO C L , et al. Ecological effect of triazophos on soil microbe[J]. Journal of Agro-Environment Science, 2008,27(1):238-242.(in Chinese with English abstract)
[18]	武贺, 段玉玺, 陈立杰 . 施用杀线剂对大豆根际真菌的影响[J]. 大豆科学, 2008,27(4):715-719.
	WU H, DUAN Y X, CHEN L J . Effect of nematicide on rhizosphere fungi in the root of soybean[J]. Soybean Science, 2008,27(4):715-719.(in Chinese with English abstract)
[19]	鲍士旦 . 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[20]	刘恩太, 李园园, 胡艳丽 , 等. 棉隆对苹果连作土壤微生物及平邑甜茶幼苗生长的影响[J]. 生态学报, 2014,34(4):847-852. DOI URL
	LIU E T, LI Y Y, HU Y L , et al. Effects of dazomet on edaphon and growth of Malus hupehensis Rehd. under continuous apple cropping[J]. Acta Ecologica Sinica, 2014,34(4):847-852.(in Chinese with English abstract)
[21]	孙秀山, 封海胜, 万书波 , 等. 连作花生田主要微生物类群与土壤酶活性变化及其交互作用[J]. 作物学报, 2001,27(5):617-621.
	SUN X S, FENG H S, WAN S B , et al. Changes of main microbial strains and enzymes activities in peanut continuous cropping soil and their interactions[J]. Acta Agronomica Sinica, 2001,27(5):617-621.(in Chinese with English abstract)
[22]	吴海燕, 范作伟, 刘春光 , 等. 保护性耕作条件下玉米田土壤微生物区系变化与影响因素分析[J]. 玉米科学, 2008,16(4):135-139.
	WU H Y, FAN Z W, LIU C G , et al. Analysis on the regularity and influence factors of change of soil micro flora under maize planting technology of conservation tillage[J]. Journal of Maize Sciences, 2008,16(4):135-139.(in Chinese with English abstract)
[23]	姜莉莉 . 氟醚菌酰胺对番茄四种病害的控制效果及对土壤微生物多样性的影响[D]. 泰安: 山东农业大学, 2015.
	JIANG L L . Control efficacy of LH-2010A on 4 tomato diseases and its effect on soil microbe diversity[D]. Tai'an: Shandong Agricultural University, 2015. (in Chinese with English abstract)
[24]	张盈 . 杀菌剂氟吡菌酰胺对土壤微生物群落多样性的影响[D]. 长沙: 湖南农业大学, 2013.
	ZHANG Y . Effects of fungicide fluopyram on soil microbial community diversity[D]. Changsha: Hunan Agricultural University, 2013. (in Chinese with English abstract)
[25]	LEE S, GAN J Y, KIM J S , et al. Microbial transformation of pyrethroid insecticides in aqueous and sediment phases[J]. Environmental Toxicology and Chemistry, 2004,23(1):1-6. DOI URL PMID
[26]	胡基华, 李晶, 张淑梅 , 等. 解淀粉芽孢杆菌TF28对设施连作黄瓜根际土壤酶活性和微生物的调节[J]. 江苏农业科学, 2020,48(7):152-156.
	HU J H, LI J, ZHANG S M , et al. Regulation of Bacillus amyloliquefaciens TF28 on soil enzyme activities and microorganisms in rhizosphere of cucumber under continuous cropping in greenhouse[J]. Jiangsu Agricultural Sciences, 2020,48(7):152-156.(in Chinese)
[27]	GOMES N C M, FAGBOLA O, COSTA R , et al. Dynamics of fungal communities in bulk and maize rhizosphere soil in the tropics[J]. Applied and Environmental Microbiology, 2003,69(7):3758-3766. DOI URL PMID
[28]	董艳, 董坤, 郑毅 , 等. 种植年限和种植模式对设施土壤微生物区系和酶活性的影响[J]. 农业环境科学学报, 2009,28(3):527-532.
	DONG Y, DONG K, ZHENG Y , et al. Soil microbial community and enzyme activities in greenhouse with different cultivation years and planting system[J]. Journal of Agro-Environment Science, 2009,28(3):527-532.(in Chinese with English abstract)
[29]	纪春涛, 姜兴印, 房锋 , 等. 噻唑膦对冬暖式大棚土壤酶活性的影响[J]. 农药学学报, 2009,11(1):137-140.
	JI C T, JIANG X Y, FANG F , et al. Effects of fosthiazate on activity of soil enzymes in vegetable greenhouse[J]. Chinese Journal of Pesticide Science, 2009,11(1):137-140.(in Chinese with English abstract)
[30]	纪春涛 . 噻唑膦对土壤酶活性及黄瓜幼苗安全性研究[D]. 泰安: 山东农业大学, 2009.
	JI C T . Effects of fosthiazate to soil enzyme and cucumber seedling[D]. Tai'an: Shandong Agricultural University, 2009. (in Chinese with English abstract)
[31]	傅丽君, 杨文金 . 4种农药对枇杷园土壤磷酸酶活性及微生物呼吸的影响[J]. 中国生态农业学报, 2007,15(6):113-116.
	FU L J, YANG W J . Effects of pesticides on soil phosphatase activity and respiration of soil microorganisms in loquat orchard[J]. Chinese Journal of Eco-Agriculture, 2007,15(6):113-116.(in Chinese with English abstract)
[32]	仉欢, 朱玉坤, 乔康 , 等. 磷化铝对土壤微生物数量和酶活性的影响[J]. 农业环境科学学报, 2012,31(1):143-148.
	ZHANG H, ZHU Y K, QIAO K , et al. Effects of aluminum phosphide on soil microbial population and enzyme activities[J]. Journal of Agro-Environment Science, 2012,31(1):143-148.(in Chinese with English abstract)
[33]	贾红梅, 方千, 张秫华 , 等. AM真菌对丹参生长及根际土壤酶活性的影响[J]. 草业学报, 2020,29(6):83-92.
	JIA H M, FANG Q, ZHANG S H , et al. Effects of AM fungi on growth and rhizosphere soil enzyme activities of Salvia miltiorrhiza[J]. Acta Prataculturae Sinica, 2020,29(6):83-92.(in Chinese with English abstract)
[34]	颜冬冬, 王秋霞, 郭美霞 , 等. 4种熏蒸剂对土壤氮素转化的影响[J]. 中国生态农业学报, 2010,18(5):934-938.
	YAN D D, WANG Q X, GUO M X , et al. Effects of four fumigants on soil nitrogen transformation[J]. Chinese Journal of Eco-Agriculture, 2010,18(5):934-938.(in Chinese with English abstract)

处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	第一次用药The first time		第二次用药The second time
处理 Treatment	药剂Reagent	用量Dose	药剂Reagent	用量Dose
CK	—	—	—	—
T1	AG	3 g·m^-2	AM	2.25 mL·m^-2
T2	Fl	0.15 mL·m^-2	—	—
T3	Vc	7.5 g·m^-2	—	—
T4	Pl	0.75 g·m^-2	Pl	0.75 g·m^-2
T5	Ho	6 mL·m^-2	—	—
T6	tc	0.15 g·m^-2	—	—

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.25±0.05 a	1.47±0.32 a	18.00±1.12 ab	40.90±4.45 a	1.64±0.14 a
T1	4.43±0.12 a	1.67±0.32 a	27.73±0.67 a	37.37±3.49 a	0.51±0.01 c
T2	4.45±0.08 a	1.33±0.17 a	11.07±0.54 b	35.43±5.60 a	0.55±0.02 bc
T3	4.43±0.05 a	1.37±0.17 a	24.50±2.60 a	41.23±1.17 a	0.30±0.04 d
T4	4.34±0.07 a	2.07±0.35 a	17.27±2.48 ab	41.53±1.28 a	0.61±0.05 bc
T5	4.62±0.29 a	1.77±0.38 a	22.17±1.24 a	41.37±0.80 a	0.64±0.03 bc
T6	4.72±0.21 a	1.30±0.15 a	24.37±7.16 a	35.27±2.42 a	0.73±0.07 b

处理 Treatment	pH	有机质 Organic matter/%	铵态氮 Ammonium nitrogen/ (mg·kg^-1)	速效钾 Available potassium/ (mg·kg^-1)	速效磷 Available phosphorus/ (mg·kg^-1)
CK	4.77±0.17 ab	0.10±0.01 a	5.67±0.48 e	41.45±2.51 e	ND
T1	5.27±0.02 a	0.13±0.08 a	42.37±1.59 b	74.50±1.33 c	1.00±1.00 a
T2	4.66±0.18 b	0.30±0.06 a	35.57±1.21 c	55.73±4.91 d	0.83±0.33 a
T3	4.71±0.09 b	0.20±0.10 a	15.17±1.13 d	74.03±6.11 c	0.47±0.47 a
T4	4.98±0.21 ab	0.17±0.12 a	14.00±1.79 d	89.55±1.36 b	0.33±0.17 a
T5	4.44±0.25 b	0.13±0.07 a	72.83±4.37 a	108.00±4.62 a	1.73±0.72 a
T6	4.68±0.15 b	0.18±0.02 a	11.33±0.67 de	99.33±0.67 ab	ND

Effects of six kinds of nematicides on soil microbial population, enzymes activities and nutrients in replanted Chinese yam field

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[13]	ZHU Zhen\\|ling1，2， MA Wan\\|zhu2， REN Zhou\\|qiao2， SHEN Jian\\|guo3， LONG Wen\\|li2， LYU Xiao\\|nan1，2，*. Grade division and fertility spatial distribution of cultivated land in Yuhang District#br# [J]. , 2015, 27(8): 1462-.
[14]	XU Chun\\|ping1， SUN Si\\|wen1， HAO Hui2， WANG Mo\\|ran2， MA Yu\\|ping2，*. Optimization of degradation of pectin in tobacco stem by pectinase produced from Aspergillus niger and thermal analysis of pyrolysis products [J]. , 2015, 27(4): 657-.
[15]	DU Liang1，HUANG Fang2，WANG Fei\\|fei2，LU Yao\\|bin1，2，*. The sensitivity of Aenasius bambawalei to chlorpyrifos and imidacloprid and its detoxification enzyme activities [J]. , 2015, 27(3): 387-.