Effet of vegetation restoration models on soil physiochemical properties, enzymes activities and fungal diversity in rocky desertification area of northwest Guangxi, China

doi:10.3969/j.issn.1004-1524.20220879

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (10): 2425-2435.DOI: 10.3969/j.issn.1004-1524.20220879

• Environmental Science • Previous Articles Next Articles

Effet of vegetation restoration models on soil physiochemical properties, enzymes activities and fungal diversity in rocky desertification area of northwest Guangxi, China

GAO Feng¹(), WEN Shizhi¹, WEI Shuoxing²^,^*(), OU Hanbiao², WANG Zhihui²

1. College of Forestry, Central South University of Forestry and Technology, Changsha 410004, China
2. Key Laboratory of Central South Fast-Growing Timber Cultivation of National Forestry and Grassland Administration, Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation, Guangxi Forestry Research Institute, Nanning 530002, China

Received:2022-06-13 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

In the present study, the differences of soil physiochemical properties, enzymes activities, fungal community diversity under vegetation restoration models in karst degraded areas were explored, as well as the correlation within soil physiochemical properties, enzymes activities, fungal community diversity, by taking the deciduous broad-leaved forest (DF), deciduous evergreen broad-leaved mixed forest (EDF) and evergreen broad-leaved forest (EF), which have been artificially restored for 13 years, as test treatments and the enclosure forest (ENF) as the control. It was shown that the contents of total nitrogen and available phosphorus, the activites of saccharase in the artificially restored forests were significantly (P<0.05) higher than those of ENF. The relative abundance of fungi was higher in artificially restored forest than that in ENF, in which the dominant fungi were Ascomycota and Basidiomycota. Functional prediction showed that soil fungi were mainly saprotroph and pathotroph. Compred with ENF, DF increased the relative abundance of pathotrophic fungi, EDF and EF increased the relative abundance of symbiotrophic fungi. In conclusion, compared with the enclosure forest, artificially restored forests could effectively improve soil nutrients contents, enzymes activities and relative abundance of fungal community in karst degraded areas.

Key words: vegetation restoration model, fungal community, karst, enzyme activity, soil nutrient

CLC Number:

GAO Feng, WEN Shizhi, WEI Shuoxing, OU Hanbiao, WANG Zhihui. Effet of vegetation restoration models on soil physiochemical properties, enzymes activities and fungal diversity in rocky desertification area of northwest Guangxi, China[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2425-2435.

Figures/Tables 7

Table 1 Basic information of sample areas

指标 Index	自然封育林 Enclosure forest	落叶阔叶林 Deciduous broad- leaved forest	落叶常绿阔叶混交林 Evergreen deciduous broad-leaved forest	常绿阔叶林 Evergreen broad- leaved forest
海拔Elevation/m	291~339	287~337	288~337	286~337
坡度Slope/(°)	33	24	24	24
坡向Aspect	东南Southeast	东南Southeast	东南Southeast	东南Southeast
土壤类型Soil type	钙质土Calcareous soil	钙质土Calcareous soil	钙质土Calcareous soil	钙质土Calcareous soil
恢复时间Recovery time/a	13	13	13	13
平均树高Mean height of trees/m	—	12.1	9.2	9.1
平均胸径	—	13.2	8.9	8.4
Average diameter at breast height of trees/cm
郁闭度Canopy density/%	—	80	85	90
腐殖质层厚度	4~6	8~10	6~8	3~5
Thickness of humus layer/cm
土壤厚度Thickness of soil/cm	50~70	40~60	40~60	40~60
物种组成Species composition	VN、MB、JN、RC	ZI、CA、LL、RC、VN	CA、DT、ZI、CP、VN、MB	QG、RC、JN、FT

Table 2 Soil physiochemical properties under vegetation restoration models

植被类型	SOC/(g·kg^-1)	TN/(g·kg^-1)	AK/(g·kg^-1)	AN/(mg·kg^-1)	AP/(mg·kg^-1)	pH
Vegetation type
DF	90.39±0.12 a	5.26±0.09 a	243.33±2.50 a	354.53±2.51 a	6.79±0.20 b	7.57±0.13 a
EDF	89.84±1.00 a	5.11±0.11 a	160.75±0.73 c	274.13±1.97 b	9.13±0.35 a	6.66±0.17 b
EF	87.58±1.05 b	5.10±0.06 a	153.74±0.79 d	256.33±4.59 d	4.00±0.44 c	6.59±0.06 b
ENF	86.61±0.93 b	4.56±0.28 b	167.48±1.35 b	263.20±2.33 c	3.36±0.17 d	7.72±0.09 a

Fig.1 Soil enzymes activities under vegetation restoration models CAT, Catalase; SAC, Saccharase; URE, Urease; ALP, Alkaline phosphatase. ENF, Enclosure forest; DF, Deciduous broad-leaved forest; EDF, Evergreen deciduous broad-leaved forest; EF, Evergreen broad-leaved forest. The same as below. Bars marked without the same letters indicate significant difference at P<0.05.

Fig.2 OTU (operational taxonomic unit) abundance accumulation histogram of soil dominant fungal species at phylum level

Fig.3 Similarity analysis box diagram (left) and principal coordinate analysis diagram (right) PC1, Principle component 1; PC2, Principle component 2.

Fig.4 Nutrient patterns of soil fungi under vegetation models based on FUNGuide predictions

Fig.5 Network analysis of soil nutrients, enzymes activities and fungal communities SOC, Soil organic carbon; TN, Total nitrogen; AK, Available potassium; AN, Available nitrogen; AP, Available phosphorus. The green dots in the figure represent the dominant species of fungi. The size of the dots indicates the relative abundance of the dominant species. The red dots represent the indicators of soil enzymes activities, and the grey dots represent the indicators of soil nutrients. The blue lines indicate significant (P<0.05) positive correlations, and the red lines indicate significant (P<0.05) negative correlations, and the redder or bluer indicates higher correlation coefficients.

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Effet of vegetation restoration models on soil physiochemical properties, enzymes activities and fungal diversity in rocky desertification area of northwest Guangxi, China

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