Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers

doi:10.3969/j.issn.1004-1524.2019.03.10

›› 2019, Vol. 31 ›› Issue (3): 414-419.DOI: 10.3969/j.issn.1004-1524.2019.03.10

• Horticultural Science • Previous Articles Next Articles

Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers

WANG Weike, YUAN Weidong, LU Na, SONG Jiling, YAN Jing

Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

Received:2018-08-06 Online:2019-03-25 Published:2019-04-08

Abstract

Abstract: In order to provide basis for protection and development of germplasm resources of wild Sanghuangporus sanghuang, the genetic diversity of 17 wild Sanghuangporus sanghuang strains was investigated in this study. The amplified products of 17 wild Sanghuangporus sanghuang strains DNA based on ISSR makers technique were used to analyze the genetic diversity of Sanghuangporus sanghuang. Cluster dendrogram of different samples were established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software. The results showed that bands amplified by 10 ISSR primers among the 16 ISSR primers were clear and polymorphisms rich. 10 ISSR primers generated 904 loci, of which 717 loci were polymorphic. The percentage of polymorphisms was 79.3%. Bands amplified by ISSR-primers P5 and P812 had the highest polymorphisms in DNA fingerprint. The genetic similarity coefficients of 17 Sanghuangporus sanghuang strains ranged from 0.57 to 0.99. UPGMA cluster analysis showed that 17 Sanghuangporus sanghuang strains were divided into 2 groups at the level of genetic similarity coefficient (GS) about 0.65 in the cluster dendrogram. S4, S23, S26 were grouped together and the other 14 strains were in one group. Results of ISSR analysis revealed that germplasm resources of Sanghuangporus sanghuang had plentiful genetic diversity. ISSR method was efficient for distinguishing different strains of Sanghuangporus sanghuang.

Key words: Sanghuangporus sanghuang, inter simple sequence repeat, genetic diversity

CLC Number:

S567.3⁺9

WANG Weike, YUAN Weidong, LU Na, SONG Jiling, YAN Jing. Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers[J]. , 2019, 31(3): 414-419.

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Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers

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