Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker

doi:10.3969/j.issn.1004-1524.2020.04.08

›› 2020, Vol. 32 ›› Issue (4): 616-623.DOI: 10.3969/j.issn.1004-1524.2020.04.08

• Horticultural Science • Previous Articles Next Articles

Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker

GUO Qinwei¹, ZHANG Ting¹, LIU Huiqin¹, ZHANG Xinhui¹, LI Chaosen¹, XIANG Xiaomin¹, ZHAO Dongfeng¹, WAN Hongjian^2,*

1.Quzhou Academy of Agircultural Sciences, Quzhou 324000, China;
2.Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-09-29 Online:2020-04-25 Published:2020-04-26

Abstract

Abstract: The genetic diversity of 73 Luffa germplasms was analyzed with inter-simple sequence repeat(ISSR) molecular markers. Fifteen pairs of ISSR primers were screened out of 60 ISSR primer pairs, which produced 99 DNA bands, the average number of polymorphic DNA bands amplified by each primer pair was 6.6, 92 polymorphic bands, 7 basic bands, the proportion of polymorphic primers ranged from 66.67% to 100% and the ratio of polymorphic bands was 92.90%.The results showed the high genetic diversity of Luffa germplasm resources. The value of Nei’s genetic similarity (GS) indexes of 73 Luffa germplasm varied from 0.454 5 to 0.959 6. Clutser analysis with UPGMA method showed that the 73 Luffa germplasm were clustered into 2 groups, Luffa cylindrica(L.) Roem and Luffa acutangula(L.) Roxb., with the similarity coefficient of 0.59. However, with the similarity coefficient of 0.66, these Luffa germplasm were clustered into 4 groups, including tinea fruit, green or light green fruit types, short stick types from west Zhejiang province and long stick types from Guangdong and Guangxi areas.

Key words: inter-simple sequence repeat(ISSR), germplasm resource, genetic diversity, Luffa

CLC Number:

S642.4

GUO Qinwei, ZHANG Ting, LIU Huiqin, ZHANG Xinhui, LI Chaosen, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker[J]. , 2020, 32(4): 616-623.

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Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker

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