Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence

doi:10.3969/j.issn.1004-1524.2020.05.08

›› 2020, Vol. 32 ›› Issue (5): 810-815.DOI: 10.3969/j.issn.1004-1524.2020.05.08

• Horticultural Science • Previous Articles Next Articles

Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence

YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen^*, WEI Shenghua

Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

Received:2019-11-14 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: System evolution relationship, genetic diversity and molecular identification method of the germplasm resources of Sabia parviflora from different regions of Guizhou were analyzed based on the sequence of psbA-trnH chloroplast gene. The psbA-trnH sequences of 13 Sabia parviflora samples from 4 counties and districts in Guizhou were amplified and sequenced by PCR. Codon Code Aligner 8.0.2 software was applied for collation and splicing, and population genetic distance, haplotype number and diversity were analyzed to construct phylogenetic tree. The length of psbA-trnH sequence of 13 chloroplast genes of Sabia parviflora was 469-488 bp, with 37 polymorphism sites, 14 reduced information sites, 23 indigenous sites, and 3 inserted/deleted fragments. The variation range of genetic distance between different populations was 0-0.085 9, and the average genetic distance was 0.025 3, with 4 haplotypes, haplotype (gene) diversity (Hd) of 0.679. In Fu and Li's D^* test, Fu and Li's F^* test, and Tajima's D test, P values were all greater than 0.10, without statistical significance. In conclusion, the evolution rate of Sabia parviflora was not consistent, and the genetic diversity was rich. This study enriched the research data of Sabia parviflora and its genera, and provided a certain reference for the classification, identification, systematic evolution and molecular marker development of Sabia parviflora.

Key words: Sabia parviflora, psbA-trnH sequence, Guizhou, genetic diversity

CLC Number:

S567

YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence[J]. , 2020, 32(5): 810-815.

References

[1] 吴征镒,洪德元.Flora of China:第12卷[M].北京:科学出版社, 2009.
[2] 张雪晴, 李曼姝, 李玉玲, 等. 我国清风藤属药用植物研究概况[J]. 生物资源, 2018, 40(6): 477-490.
ZHANG X Q, LI M S, LI Y L, et al.Research progress on Sabia medicinal plants in China[J]. Biotic Resources, 2018, 40(6): 477-490.(in Chinese with English abstract)
[3] 贵州省药品监督管理局. 贵州省中药材、民族药材质量标准[S].贵阳:贵州科技出版社,2003.
[4] 何顺志, 徐文芬. 贵州中草药资源研究[M]. 贵阳: 贵州科技出版社, 2007.
[5] 陈谨, 邓赟, 唐天君, 等. 小花清风藤三萜成分的研究[J]. 中草药, 2004, 35(1): 16-17.
CHEN J, DENG Y, TANG T J, et al.Study on the constituents of triterpenoids of Sabia parviflora[J]. Chinese Traditional and Herbal Drugs, 2004, 35(1): 16-17.(in Chinese)
[6] 赵兰君, 王玉伟, 李志峰, 等. 小花清风藤化学成分的分离与鉴定[J]. 中草药, 2018, 49(3): 544-548.
ZHAO L J, WANG Y W, LI Z F, et al.Isolation and identification of chemical constituents from Sabia parviflora[J]. Chinese Traditional and Herbal Drugs, 2018, 49(3): 544-548.(in Chinese with English abstract)
[7] 李曼姝, 李建桥, 胡建忠, 等. 小花清风藤的化学成分及其对肝细胞的保护作用[J]. 生物资源, 2018, 40(6): 491-494.
LI M S, LI J Q, HU J Z, et al.Chemical constituents of Sabia parviflora and their hepatocyte protection activity[J]. Biotic Resources, 2018, 40(6): 491-494.(in Chinese with English abstract)
[8] 杨莹, 张永萍, 梁光义. 小花清风藤胶囊的保肝作用研究及其急毒实验[J]. 中国现代应用药学, 2013, 30(11): 1166-1170.
YANG Y, ZHANG Y P, LIANG G Y.Protective effect of Xiaohuaqingfengteng capsule on acute liver injury mice and its acute toxicity test[J]. Chinese Journal of Modern Applied Pharmacy, 2013, 30(11): 1166-1170.(in Chinese with English abstract)
[9] FUNABASHI M. Augmentation of plant genetic diversity in synecoculture: theory and practice in temperate and tropical zones[M]//Sustainable Development and Biodiversity. Cham: Springer International Publishing, 2019: 3-46.
[10] SHI X W, GONG C, ZHANG L, et al.Which species should we focus on? umbrella species assessment in southwest China[J]. Biology, 2019, 8(2): 42.
[11] 高连明. DNA条形码在生物多样性编目与评价中的应用[J]. 生物多样性, 2015, 23(3): 286-287.
GAO L M.Applications of DNA barcoding in biodiversity inventory and assessment[J]. Biodiversity Science, 2015, 23(3): 286-287.(in Chinese)
[12] INTHARUKSA A, SASAKI Y, ANDO H, et al.The combination of ITS2 and psbA-trnH region is powerful DNA barcode markers for authentication of medicinal Terminalia plants from Thailand[J]. Journal of Natural Medicines, 2020, 74(1): 282-293.
[13] 赵海, 李园园, 刘红昌, 等. 基于ITS2和psbA-trnH序列的黔太子参品种DNA条形码鉴定研究[J]. 中药材, 2018, 41(4): 855-858.
ZHAO H, LI Y Y, LIU H C, et al.DNA barcoding identification of Pseudostellaria heterophylla based on ITS2 and psba-trnh sequences[J]. Journal of Chinese Medicinal Materials, 2018, 41(4): 855-858.(in Chinese)
[14] 张宏意, 袁林林, 饶秋红, 等. 不同产地何首乌叶绿体psbA-trnH基因序列分析[J]. 中草药, 2018, 49(5): 1146-1149.
ZHANG H Y, YUAN L L, RAO Q H, et al.Sequence analysis of psbA-trnH gene in chloroplast of Polygonum multiflorum from different producing areas[J]. Chinese Traditional and Herbal Drugs, 2018, 49(5): 1146-1149.(in Chinese with English abstract)
[15] 林爽, 吴海燕, 张宏意, 等. 不同地理居群破布叶的psbA-trnH和ITS2序列及其聚类分析[J]. 中草药, 2017, 48(7): 1403-1408.
LIN S, WU H Y, ZHANG H Y, et al.Cluster analysis on psbA-trnH and ITS2 sequences of Microcos paniculata from different geographical populations[J]. Chinese Traditional and Herbal Drugs, 2017, 48(7): 1403-1408.(in Chinese with English abstract)
[16] KUMAR S, STECHER G, TAMURA K.MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 2016, 33(7): 1870-1874.
[17] ROZAS J, SANCHEZ-DELBARRIO J C, MESSEGUER X, et al. DnaSP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics, 2003, 19(18): 2496-2497.

Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Wenqiang, WANG Xiaofu, CHEN Xiaoyun, PENG Cheng, XU Junfeng, CAI Jian. Preliminary study on identification of Dendrobium officinale from Zhejiang based on ITS2 and SNP technology [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 69-76.
[2]	LI Lei, ZHAO Huajin, WU Zitao, LI Shenghe, JIANG Jinpeng, LIU Chang. Network pharmacological analysis of anti-oxidant mechanism of Cassia semen [J]. , 2020, 32(10): 1855-1865.
[3]	WU Yan, QIAO Xiaoyan, GE Weiqiang, GAO Qinghai. Effects of exogenous melatonin on physiological and biochemical characteristics in female flowers of Trichosanthes kirilowii under high temperature and strong light [J]. , 2020, 32(3): 421-429.
[4]	LIU Yuxuan, CHENG Huan, YE Xingqian, LIU Huiyan, FANG Haitian. Changes of bioactive compounds and volatile compounds contents in goji juice fermented by different probiotics [J]. , 2020, 32(3): 499-509.
[5]	WANG Hengxu, XU Weihui, YANG Youcai, WANG Zhigang, LIU Zeping, WANG Kexin. Screening, identification, and characteristics of an antagonistic strain against Fusarium oxysporum f.sp. niveum [J]. , 2019, 31(10): 1671-1680.
[6]	YUAN Xiaolong, LI Yunqin, WANG Yi. Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi [J]. , 2019, 31(9): 1478-1484.
[7]	WANG Yiming, LONG Shengju, CHEN Yan, HE Zhongqun, ZHAO Yingpeng, YANG Luxi, YAN Wenyi. Effect of soil simulated acidification on photosynthetic characteristics and ultrastructure of Sedum aizoon L. leaves [J]. , 2019, 31(6): 915-921.
[8]	WANG Weike, YUAN Weidong, LU Na, SONG Jiling, YAN Jing. Genetic diversity of wild Sanghuangporus sanghuang based on ISSR molecular markers [J]. , 2019, 31(3): 414-419.
[9]	WANG Weike, SONG Jiling, WU Youliang, YAN Jing, LU Na, YUAN Weidong, CHEN Guanping. Molecular identification and genetic diversity of a traditional Chinese medicinal fungus Sanghuang [J]. , 2019, 31(2): 307-314.
[10]	YIN Minghua, ZHAN Xuelin, XU Wenhui, XIE Nini, CAI Hong, CHEN Ronghua. Genetic diversity analysis of Tetrastigma hemsleyanum Diels et Gilg germplasm resources by random amplified polymorphic DNA technique [J]. , 2018, 30(11): 1839-1848.
[11]	TENG Yao, LI Anding, HAO Ziyuan, ZHANG Hongliang, ZHANG Limin, CAI Guojun. Anatomical structure of Passiflora caerulea L. and relationship between leaf structure and cold resistance under low temperature stress [J]. , 2018, 30(11): 1849-1858.
[12]	CHEN Zhi, WANG Yiting, LYU Yongping, MU Haojie, CHEN Jianping. Optimization of aseptic seeding system of Dendrobium officinale Kimura et Migo [J]. , 2018, 30(11): 1870-1878.
[13]	MA Lifen, YIN Yue, ZHAO Jianhua, DAI Guoli, AN Wei. Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers [J]. , 2018, 30(10): 1665-1670.
[14]	SUN Caixia, PAN Zhiyan, DAI Fen, ZHENG Weiran, YU Guoguang. Raman spectroscopic analysis and identification of Thunberg Fritillary Bulb [J]. , 2018, 30(7): 1244-1248.
[15]	HONG Senrong, NING Bensong, YE Siyu, LIU Yan, ZHANG Mingxin, ZHAN Xuelin. Dark culture of embryogenic calli after cryopreservation and semithin section observation of its regenerated plantlet of Dioscorea bulbifera L. [J]. , 2018, 30(1): 65-70.