Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers

doi:10.3969/j.issn.1004-1524.2018.10.08

›› 2018, Vol. 30 ›› Issue (10): 1665-1670.DOI: 10.3969/j.issn.1004-1524.2018.10.08

• Horticultural Science • Previous Articles Next Articles

Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers

MA Lifen¹, YIN Yue², ZHAO Jianhua², DAI Guoli², AN Wei^{2, *}

1. Wolfberry Industry Development Centre of Ningxia, Yinchuan 750001, China;
2.National Wolfberry Engineering Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China

Received:2017-11-21 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: Genetic diversity of 17 goji (wolfberry) cultivars was analyzed by start codon targeted polymorphism (SCoT) molecular marker. Nineteen SCoT primers generating clear and reproducible bands were obtained through screening a total of eighty primers. A total of 96 bands were amplified, of which 71 (73.96%) were polymorphic. The value of polymorphism information content (PIC) varied from 0.055 (SCoT-29) to 0.239 (SCoT-66), with average of 0.132. Cluster analysis and principal coordinated analysis indicated that the genetic similarity (GS) varied from 0.43 to 1.00. All cultivars could be divided into three groups at the genetic similarity of 0.87. So SCoT markers could be effectively used to evaluate the genetic diversity of goji (wolfberry) cultivars germplasms. These results would provide references for protection and rational utilization of wolfberry cultivars.

Key words: goji (wolfberry), cultivar, SCoT marker, genetic diversity

CLC Number:

S567.19

MA Lifen, YIN Yue, ZHAO Jianhua, DAI Guoli, AN Wei. Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers[J]. , 2018, 30(10): 1665-1670.

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Analysis on genetic diversity of 17 goji(wolfberry)cultivars by SCoT markers

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