Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker

doi:10.3969/j.issn.1004-1524.2019.10.07

›› 2019, Vol. 31 ›› Issue (10): 1624-1631.DOI: 10.3969/j.issn.1004-1524.2019.10.07

• Horticultural Science • Previous Articles Next Articles

Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker

ZHANG Anshi¹, PANG Jincheng¹, LIU Ying¹, GAO Dengtao², WEI Zhifeng^2,*

1. School of Science, Jiaozuo Teachers College, Jiaozuo 454000, China;
2. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009,China

Received:2019-04-03 Online:2019-10-25 Published:2019-10-30

Abstract

Abstract: In order to explore the genetic relationship among the germplasm resources of Muscat Hamburg grapevine series, the genetic diversity of 39 cultivars was analyzed by iPBS marker, and their DNA fingerprints were constructed. 14 primers were screened for PCR amplification of 39 cultivars. A total of 152 bands were generated, of which 132 bands were polymorphic bands (86.86%).The average value of polymorphism information content, number of observed alleles, number of effective alleles, Nei's gene diversity index and Shannon's information index were 0.863 8, 1.868 4, 1.409 0, 0.251 8 and 0.390 7, respectively. The genetic similarity coefficient among 39 cultivars ranged from 0.526 3 to 0.940 8 with a range of 0.413 5. The above results indicated that there were rich genetic diversity among 39 cultivars. The dendrogram of these 39 cultivars was constructed using UPGMA. They could be divided into 4 groups at genetic similarity coefficient of 0.72, of which V. vinifera were mainly distributed in Group Ⅰ, while the Euro-American hybrids species were mainly distributed in Groups Ⅱ, Ⅲ and Ⅳ. The DNA fingerprints of 39 cultivars were constructed using 16 polymorphic loci amplified of 8 primers, providing scientific basis for the identification of Muscat Hamburg grapevine series.

Key words: grape, iPBS, DNA fingerprint, genetic diversity

CLC Number:

S663.1

ZHANG Anshi, PANG Jincheng, LIU Ying, GAO Dengtao, WEI Zhifeng. Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker[J]. , 2019, 31(10): 1624-1631.

References

[1] 王娟, 陶永焕, 宋尚伟. 葡萄EST-SSR引物的开发及部分种质聚类分析[J]. 华北农学报, 2014, 29(2): 121-126.
WANG J, TAO Y H, SONG S W.Development of EST-SSR primers and cluster analysis of some cultivars in grape[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(2): 121-126.(in Chinese with English abstract)
[2] 孟聚星, 姜建福, 张国海, 等. 我国育成的葡萄新品种系谱分析[J]. 果树学报, 2017, 34(4): 393-409.
MENG J X, JIANG J F, ZHANG G H, et al.Pedigree analysis of grape cultivars released in China[J]. Journal of Fruit Science, 2017, 34(4): 393-409.(in Chinese with English abstract)
[3] 陶然, 王晨, 房经贵, 等. 我国葡萄育种研究概况[J]. 江西农业学报, 2012, 24(6): 24-30.
TAO R, WANG C, FANG J G, et al.General situation of grape breeding research in China[J]. Acta Agriculturae Jiangxi, 2012, 24(6): 24-30.(in Chinese with English abstract)
[4] KALENDAR R, ANTONIUS K, SMyKAL P, et al. iPBS: a universal method for DNA fingerprinting and retrotransposon isolation[J]. Theoretical and Applied Genetics, 2010, 121(8): 1419-1430.
[5] KALENDAR R, FLAVELL A J, ELLIS T N, et al.Analysis of plant diversity with retrotransposon-based molecular markers[J]. Heredity, 2011, 106(4): 520-530.
[6] ANDEDEN E E, BALOCH F S, DERYA M, et al.iPBS-Retrotransposons-based genetic diversity and relationship among wild annual Cicer species[J]. Journal of Plant Biochemistry and Biotechnology, 2013, 22(4): 453-466.
[7] RUNGIS D, GAILE I, VEINBERGA I, et al. Use of DNA markers for cereal line uniformity assessment[J]. Proceedings of the Latvian Academy of Sciences, 2012, 66(1/2): 21-25.
[8] BARÁNEK M, MESZÁROS M, SOCHOROVÁ J, et al. Utility of retrotransposon-derived marker systems for differentiation of presumed clones of the apricot cultivar Velkopavlovick[J]. Scientia Horticulturae, 2012, 143: 1-6.
[9] ŽIAROVSKÁ J, FERNÁNDEZ E C, BEŽO M. Developing the iPBS strategy for yakon germplasm evaluation[J]. Journal of Microbiology Biotechnology & Food Sciences, 2013, 2(Suppl.):1967-1979.
[10] 石艳艳. 葡萄早熟芽变及其亲本基因组差异分析[D]. 洛阳: 河南科技大学, 2015.
SHI Y Y.The comparing analysis of the genomic difference of grape early bud mutation and its parents[D]. Luoyang: Henan University of Science and Technology, 2015.(in Chinese with English abstract)
[11] 张潇玉, 郭大龙, 郭明晓, 等. 葡萄iPBS-PCR反应体系的优化[J]. 果树学报, 2014, 31(3): 508-513.
ZHANG X Y, GUO D L, GUO M X, et al.Optimization of iPBS-PCR system in grapevine[J]. Journal of Fruit Science, 2014, 31(3): 508-513.(in Chinese with English abstract)
[12] 杨治元. 玫瑰香系葡萄品种系谱分析[J]. 中国果树, 2006 (2):20-22.
YANG Z Y.Pedigree analysis of Muscat Hamburg grapevine series[J].China Fruits, 2006 (2):20-22.(in Chinese)
[13] 张安世, 邢智峰, 刘永英, 等. 苔藓植物DNA不同提取方法的比较分析[J]. 河南科学, 2009, 27(5): 559-562.
ZHANG A S, XING Z F, LIU Y Y, et al.Comparison and analysis on the different methods of DNA extration methods from bryophytes[J]. Henan Science, 2009, 27(5): 559-562.(in Chinese with English abstract)
[14] 黄秀, 曾捷, 聂刚, 等. 牛鞭草品种EST-SSR指纹图谱构建及遗传多样性分析[J]. 热带亚热带植物学报, 2014, 22(2): 165-171.
HUANG X, ZENG J, NIE G, et al.Construction of fingerprinting and genetic diversity analysis of Hemarthia cultivars by EST-SSR[J]. Journal of Tropical and Subtropical Botany, 2014, 22(2): 165-171.(in Chinese with English abstract)
[15] 张林, 徐迎春, 成海钟, 等. 基于ISSR标记的62个朱顶红品种的遗传关系分析及指纹图谱构建[J]. 植物资源与环境学报, 2012, 21(4): 48-54.
ZHANG L, XU Y C, CHENG H Z, et al.Genetic relationship analysis and fingerprint construction of 62 cultivars of Hippeastrum spp. based on ISSR marker[J]. Journal of Plant Resources and Environment, 2012, 21(4): 48-54.(in Chinese with English abstract)
[16] 丁洁菲, 毛娟, 赵举鹏, 等. 葡萄品种亲缘关系的RAPD标记分析[J]. 甘肃农业大学学报, 2013, 48(6): 82-87.
DING J F, MAO J, ZHAO J P, et al.Genetic relationship among grape varieties based on RAPD analysis[J]. Journal of Gansu Agricultural University, 2013, 48(6): 82-87.(in Chinese with English abstract)
[17] 李琳, 魏灵珠, 程建徽, 等. 24份葡萄种质亲缘关系的ISSR分析[J]. 浙江农业学报, 2013, 25(4): 772-776.
LI L, WEI L Z, CHENG J H, et al.Analysis of genetic relationship of 24 Vitis germplasm resources by ISSR markers[J]. Acta Agriculturae Zhejiangensis, 2013, 25(4): 772-776.(in Chinese with English abstract)
[18] 王发明, 李洁维, 叶开玉, 等. 41份葡萄种质遗传多样性的ISSR和SCoT对比分析[J]. 广西植物, 2017, 37(1): 1-8.
WANG F M, LI J W, YE K Y, et al.Comparative analysis on the genetic diversity of 41 Vitis germplasm resources by ISSR and SCoT molecular markers[J]. Guihaia, 2017, 37(1): 1-8.(in Chinese with English abstract)
[19] 李雪雁, 梁海永, 宪立杰, 等. 基于SSR技术对七十五份栽培葡萄品种的鉴定研究[J]. 北方园艺, 2015(13): 115-119.
LI X Y, LIANG H Y, XIAN L J, et al.Identification of SSR in 75 cultivated grape varieties[J]. Northern Horticulture, 2015(13): 115-119.(in Chinese with English abstract)
[20] 郭印山, 牛早柱, 石广丽, 等. 基于SSR分子标记的葡萄品种遗传多样性分析[J]. 北方园艺, 2016(7): 89-92.
GUO Y S, NIU Z Z, SHI G L, et al.Genetic diversity analysis of grape resources based on SSR markers[J]. Northern Horticulture, 2016(7): 89-92.(in Chinese with English abstract)
[21] 王文婷. 基于EST-SSR和iPBS标记的杨梅遗传多样性研究[D]. 杭州: 浙江大学, 2016.
WANG W T.Study on genetic diversity of Chinese bayberry based on EST-SSR and iPBS markers[D]. Hangzhou: Zhejiang University, 2016.(in Chinese with English abstract)
[22] 李婵, 赵永红, 梅嘉洺, 等. 菌草种质资源iPBS多样性分析[J]. 农业科学与技术(英文版), 2016, 17(9): 2000-2004.
LI C, ZHAO Y H, MEI J M, et al.Genetic diversity of JUNCAO germplasms detected by I PBS markers[J]. Agricultural Science & Technology, 2016, 17(9): 2000-2004.

Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CUI Pengfei, WEI Lingzhu, CHENG Jianhui, XIANG Jiang, LI Mingshan, WU Jiang2, . Effects of different rootstocks on growth and fruit quality of Tiangong Cuiyu grape [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 52-61.
[2]	HONG Xia, ZHAO Yongbin, QU Weidong, CHEN Yinlong, QIU Liping, WANG Jiaoyang. Comparative analysis on genetic diversity of Colocasia esculenta germplasm in Zhejiang Province based on phenotype and simple sequence repeats markers [J]. , 2020, 32(9): 1544-1554.
[3]	GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman. Transcriptome analysis on development of aerial root in grape of Weike [J]. , 2020, 32(9): 1645-1655.
[4]	YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence [J]. , 2020, 32(5): 810-815.
[5]	GUO Qinwei, ZHANG Ting, LIU Huiqin, ZHANG Xinhui, LI Chaosen, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker [J]. , 2020, 32(4): 616-623.
[6]	ZHAO Xingkai, SHI Haichun, YU Xuejie, YANG Shu, ZHAO Changyun, XIA Wei, KE Yongpei. Breeding potential analyses of 13 new inbred lines in maize [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2119-2127.
[7]	YANG Mingxia, LIAN Hongjuan, WANG Xiaofang, DU Yiyang, DONG Zhigang, JI Wei. Identification and expression analysis of grape MPT gene family [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2173-2185.
[8]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[9]	YIN Xiaoxiao, LI Yanfang, GU Jiang, LIAO Yan, XIE Yue, YANG Guangyou, GU Xiaobin. Genetic diversity analysis of Psoroptes ovis var. cuniculi by full-length of mitochondrial ATP6 gene in China [J]. , 2019, 31(8): 1231-1238.
[10]	TANG Shoujie, BI Xiang, ZHANG Feiming, ZHANG Youliang. Genetic diversity analysis and RAPD identification of three successive generations of meio-gynogenetic population in Megalobrama amblycephala [J]. , 2019, 31(8): 1257-1271.
[11]	YUE Gaohong, PAN Binrong, LIU Yongan, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. Genetic diversity analysis of sweet maize inbred lines in Southern Zhejiang by SSR markers [J]. , 2019, 31(7): 1029-1036.
[12]	CAO Yuehua, WEI Lingzhu, SHEN Biwei, CHENG Jianhui, XIANG Jiang, WU Jiang. Effects of rootstocks on growth and fruit quality of Xinyu grape [J]. , 2019, 31(6): 908-914.
[13]	HAN Guomin, LIU Xi, TANG Meiling, DAI Lingmin. Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress [J]. , 2019, 31(4): 556-564.
[14]	CHEN Hongqiang, XIA Hui, WANG Jin, DENG Qunxian, LIANG Dong, LYU Xiulan, TANG Liping. Identification and expression analysis of STS gene family in grape [J]. , 2019, 31(3): 401-407.
[15]	JIANG Wu, WU Shiwu, WU Zhigang, TAO Zhengming. Genetic diversity analysis of Gardenia jasminoides in Zhejiang based on SRAP molecular marker technique [J]. , 2019, 31(3): 408-413.