Breeding potential analyses of 13 new inbred lines in maize

doi:10.3969/j.issn.1004-1524.2020.12.02

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2119-2127.DOI: 10.3969/j.issn.1004-1524.2020.12.02

• Crop Science • Previous Articles Next Articles

Breeding potential analyses of 13 new inbred lines in maize

ZHAO Xingkai¹(), SHI Haichun¹^,², YU Xuejie¹^,², YANG Shu¹, ZHAO Changyun², XIA Wei², KE Yongpei¹^,²^,^*()

1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
2. Sichuan Zhenghong Bio Co., Ltd., Chengdu 610213, China

Received:2020-07-07 Online:2020-12-25 Published:2020-12-25
Contact: KE Yongpei

Abstract

Abstract:

In order to clarify the breeding potential of new inbred lines, genetic diversities of 13 new inbred lines, eight elite inbred lines and five standard inbred lines were studied by agronomic character identification and SSR markers. 210 combinations were obtained according to Griffing method IV, and the combining ability and heterosis of yield were analyzed. The results showed that K351 and K311 were new inbred lines with good agronomic traits per se. By using genetic similarity coefficient of 0.696 as the threshold, 26 inbred lines were divided into eight groups including five common groups and three new groups by unweighted pair-group method with arithmetic means (UPGMA), in which ten new inbred lines such as K115 were divided into three new groups. Six new inbred lines including K61 had significantly (P<0.05) positive general combining ability (GCA) effect of yield, higher than that of the most of elite inbred lines. There were 18 and three combinations with yield increase over CK significantly (P<0.05) in experiment site of Shuangliu, Sichuan and Xuanwei, Yunnan, respectively, in which K61 × K143 and 21-ES ×K61 had significant (P<0.05) yield increase at both sites, with an average increase of 33.16% and 22.81%, respectively. In summary, the new inbred lines K61, K143 and K115 had the highest GCA of yield and good agronomic traits, in which K61 and K115 were novel germplasms, they were ideal parents for high-yield breeding. K311, K962 and K365 had the higher GCA of yield and good agronomic traits, which had the potential for high-yielding breeding, however, the plant height needed to be further improved as they were kind of the higher plants.

Key words: maize, inbred lines, genetic diversity, simple sequence repeats(SSR), combining ability, heterosis

CLC Number:

S513

ZHAO Xingkai, SHI Haichun, YU Xuejie, YANG Shu, ZHAO Changyun, XIA Wei, KE Yongpei. Breeding potential analyses of 13 new inbred lines in maize[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2119-2127.

Figures/Tables 10

References 24

[1]	刘纪麟. 玉米育种学[M]. 北京: 中国农业出版社, 2002.
[2]	慈晓科, 张世煌, 谢振江, 等. 1970—2000年代玉米单交种的遗传产量增益分析方法的比较[J]. 作物学报, 2010,36(12):2185-2190. DOI URL
	CI X K, ZHANG S H, XIE Z J, et al. Comparison of analysis method of genetic yield gain for the single-cross hybrids released during 1970s—2000s[J]. Acta Agronomica Sinica, 2010,36(12):2185-2190.(in Chinese with English abstract) DOI URL
[3]	赖仲铭, 杨克诚, 雷本鸣, 等. 玉米几个自交系株型数量性状遗传的研究[J]. 中国农业科学, 1981,14(4):28-36.
	LAI C M, YANG K C, LEI B M, et al. Studies on the inheritance of quantitative characters for plant type in some inbred lines of maize[J]. Scientia Agricultura Sinica, 1981,14(4):28-36.(in Chinese with English abstract)
[4]	袁力行, 傅骏骅, 张世煌, 等. 利用RFLP和SSR标记划分玉米自交系杂种优势群的研究[J]. 作物学报, 2001,27(2):149-156.
	YUAN L X, FU J H, ZHANG S H, et al. Heterotic grouping of maize inbred lines using RFLP and SSR markers[J]. Acta Agronomica Sinica, 2001,27(2):149-156.(in Chinese with English abstract)
[5]	余世权, 苟才明, 黄宁, 等. 17个玉米地方种质选系的育种潜势分析[J]. 中国农学通报, 2010,26(22):81-86.
	YU S Q, GOU C M, HUANG N, et al. Analysis on breeding potential of 17 maize inbred lines from landraces[J]. Chinese Agricultural Science Bulletin, 2010,26(22):81-86.(in Chinese with English abstract)
[6]	乔晓, 石海春, 柯永培, 等. 玉米航天诱变系的配合力分析[J]. 四川农业大学学报, 2011,29(4):451-456.
	QIAO X, SHI H C, KE Y P, et al. Analysis of combining ability of maize mutants by space flight[J]. Journal of Sichuan Agricultural University, 2011,29(4):451-456.(in Chinese with English abstract)
[7]	秦家友, 石海春, 柯永培, 等. 玉米辐射诱变系表型及SSR遗传差异研究[J]. 玉米科学, 2012,20(2):41-47.
	QIN J Y, SHI H C, KE Y P, et al. Research on genetic difference of maize mutants by irradiation based on phenotypic and SSR[J]. Journal of Maize Sciences, 2012,20(2):41-47.(in Chinese with English abstract)
[8]	吕学高, 赵军华, 楼肖成, 等. 三十份特异玉米自交系的SSR聚类分析[J]. 浙江农业学报, 2014,26(2):274-278.
	LYU X G, ZHAO J H, LOU X C, et al. Clustering analysis of 30 specific maize inbred lines by SSR markers[J]. Acta Agriculturae Zhejiangensis, 2014,26(2):274-278.(in Chinese with English abstract)
[9]	石海春, 粟佳美, 赵长云, 等. 玉米核不育突变体K305ms主要性状配合力及产量杂种优势研究[J]. 核农学报, 2016,30(4):621-628.
	SHI H C, SU J M, ZHAO C Y, et al. Combining ability of the major characters and yield heterosis analysis of geneic male sterillity mutant K305ms in maize[J]. Journal of Nuclear Agricultural Sciences, 2016,30(4):621-628.(in Chinese with English abstract)
[10]	SINGH D. Diallel analysis over different environments-Ⅰ[J]. Indian Jouranl of Genetics & Plant Breeding, 1973,33(2):127-136.
[11]	SINGH D. Diallel analysis for combining ability over several environments-Ⅱ[J]. Indian Journal of Genetics&Plant Breeding, 1973,33(3):469-481.
[12]	荣廷昭, 潘光堂, 黄玉碧. 数量遗传学[M]. 北京: 中国科学技术出版社, 2003.
[13]	NEI M, LI W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proceedings of the National Academy of Sciences of the United States of America, 1979,76(10):5269-5273. DOI URL PMID
[14]	CLARK M S. 植物分子生物学: 实验手册[M]. 顾红雅, 译. 北京: 高等教育出版社, 1998.
[15]	ROHLF F J. NTSYS-pc: microcomputer programs for numerical taxonomy and multivariate analysis[J]. The American Statistician, 1987,41(4):330. DOI URL
[16]	聂永心, 张丽, 潘光堂, 等. 四川省常用玉米自交系SSR遗传多样性分析[J]. 分子植物育种, 2005,3(1):43-51.
	NIE Y X, ZHANG L, PAN G T, et al. Genetic diversity of dominant maize inbred lines revealed by SSR in Sichuan Province[J]. Molecular Plant Breeding, 2005,3(1):43-51.(in Chinese with English abstract)
[17]	黄青, 高明刚, 杨克诚, 等. 四川部分玉米骨干自交系杂种优势群研究[J]. 西南农业学报, 2006,19(1):19-24.
	HUANG Q, GAO M G, YANG K C, et al. Analysis of combining ability and research on the hterotic groups among elite maize inbred lines in Sichuan[J]. Southwest China Journal of Agricultural Sciences, 2006,19(1):19-24.(in Chinese with English abstract)
[18]	任纬. 21个新选及常用玉米自交系育种潜势分析[D]. 雅安: 四川农业大学, 2007.
	REN W. The breeding potential analysis of 21 new election and common maize inbred lines[D]. Ya’an: Sichuan Agricultural University, 2007.(in Chinese with English abstract)
[19]	刘世建, 杨荣, 梁燕, 等. 突破性高产优质多抗玉米杂交种雅玉889的特征特性及栽培技术[J]. 种子, 2010,29(6):107-109.
	LIU S J, YANG R, LIANG Y, et al. Characteristics and high yield cultivation technolgy of hybrid maize Yayu 889 with multiresistance etc.breakthrough merit[J]. Seed, 2010,29(6):107-109.(in Chinese)
[20]	石海春, 袁昊, 李东波, 等. 82份玉米自交系遗传多样性分析[J]. 华北农学报, 2014,29(6):84-93. DOI URL
	SHI H C, YUAN H, LI D B, et al. Analysis of genetic diversity of 82 maize inbred lines[J]. Acta Agriculturae Boreali-Sinica, 2014,29(6):84-93.(in Chinese with English abstract) DOI URL
[21]	母贵琴, 潘旭浩, 杨克诚. 3种类型玉米种质群体的育种潜势分析[J]. 玉米科学, 2011,19(3):25-30.
	MU G Q, PAN X H, YANG K C. Breeding potential analysis on three types of maize populations[J]. Journal of Maize Sciences, 2011,19(3):25-30.(in Chinese with English abstract)
[22]	马燕斌, 荣廷昭, 杨克诚, 等. 6个玉米人工合成群体的育种潜势分析[J]. 中国农业科学, 2007,40(8):1594-1601.
	MA Y B, RONG T Z, YANG K C, et al. Breeding potential analysis of six synthetic maize populations[J]. Scientia Agricultura Sinica, 2007,40(8):1594-1601.(in Chinese with English abstract)
[23]	冯志前, 王博新, 徐淑兔, 等. 12份美国玉米自交系配合力评价[J]. 玉米科学, 2020,28(2):11-17.
	FENG Z Q, WANG B X, XU S T, et al. Evaluation of the combining ability on 12 American maize inbred lines[J]. Journal of Maize Sciences, 2020,28(2):11-17.(in Chinese with English abstract)
[24]	郭章贤, 卢华兵, 郭国锦. 甜玉米自交系主要数量性状配合力分析[J]. 浙江农业学报, 2006,18(5):382-386.
	GUO Z X, LU H B, GUO G J. Analysis of combining ability of major quantitative characters in sweet maize inbred lines[J]. Acta Agriculturae Zhejiangensis, 2006,18(5):382-386.(in Chinese with English abstract)

编号 No.	名称 Name	来源 Origin	编号 No.	名称 Name	来源 Origin
1	K115	美国杂交种American hybrid	14	698-3	美国杂交种78698 American hybrid 78698
2	K492	USQ1群体USQ1 Population	15	K169	美国杂交种3163 American hybrid 3163
3	K131	USQ1群体 USQ1 Population	16	K236	美国杂交种3163 American hybrid 3163
4	K351	先玉335 Xianyu335	17	R08	美国杂交种78641 American hybrid 78641
5	K962	先玉696 Xianyu696	18	郑58	掖478亲本变异株 Mutant plant of Ye478
6	K311	泰玉3号Taiyu3	19	K305	美国杂交种2102 American hybrid 2102
7	K143	雅玉889 Yayu889	20	48-2	(Mo17×D340)×6个杂交组合 (Mo17×D340)×6 combinations
8	K324	良玉99 Liangyu99	21	21-ES	地方品种邻水大督督 Local variety Lingshuidadudu
9	K61	邦豪玉108号 Banghaoyu108	22	Mo17	187-2×C103
10	K181	良玉118 Liangyu118	23	丹340	白骨旅9×有稃玉米(辐射) Baigulyu9×Wild pod corn(radiation)
11	K322	德国杂交种 German hybrid	24	昌7-2	昌单7号(黄早四×潍95) Changdan7 (Huangzaosi×Wei95)
12	K111	国内杂交种 Chinese hybrid	25	掖478	U8112×沈5003 U8112×Shen5003
13	K365	MCOⅡ群体 MCOⅡ population	26	齐319	美国杂交种78599 American hybrid 78599

编号 No.	名称 Name	来源 Origin	编号 No.	名称 Name	来源 Origin
1	K115	美国杂交种American hybrid	14	698-3	美国杂交种78698 American hybrid 78698
2	K492	USQ1群体USQ1 Population	15	K169	美国杂交种3163 American hybrid 3163
3	K131	USQ1群体 USQ1 Population	16	K236	美国杂交种3163 American hybrid 3163
4	K351	先玉335 Xianyu335	17	R08	美国杂交种78641 American hybrid 78641
5	K962	先玉696 Xianyu696	18	郑58	掖478亲本变异株 Mutant plant of Ye478
6	K311	泰玉3号Taiyu3	19	K305	美国杂交种2102 American hybrid 2102
7	K143	雅玉889 Yayu889	20	48-2	(Mo17×D340)×6个杂交组合 (Mo17×D340)×6 combinations
8	K324	良玉99 Liangyu99	21	21-ES	地方品种邻水大督督 Local variety Lingshuidadudu
9	K61	邦豪玉108号 Banghaoyu108	22	Mo17	187-2×C103
10	K181	良玉118 Liangyu118	23	丹340	白骨旅9×有稃玉米(辐射) Baigulyu9×Wild pod corn(radiation)
11	K322	德国杂交种 German hybrid	24	昌7-2	昌单7号(黄早四×潍95) Changdan7 (Huangzaosi×Wei95)
12	K111	国内杂交种 Chinese hybrid	25	掖478	U8112×沈5003 U8112×Shen5003
13	K365	MCOⅡ群体 MCOⅡ population	26	齐319	美国杂交种78599 American hybrid 78599

变异来源 Source of variance	自由度 df	株高 Plant height	穗位高 Ear height	雄穗分支数 Tassel branch No.	穗长 Ear length	秃尖长 Barren ear tip length	穗粗 Ear diameter	穗行数 Ear row No.	行粒数 Kernels per row	百粒重 100-kernel weight	出籽率 Seed-producing percentage	产量 Yield
自交系间 Inbred line	12	26 496.22^**	10 414.84^**	446.90^**	88.81^**	3.02^**	3.59^**	73.26^**	313.36^**	63.69^**	1 052.19^**	6 317.38^**

误差 Error	355	206.44	100.50	4.41	1.59	0.32	0.06	2.10	12.24	3.82	16.13	300.45

变异来源 Source of variance	自由度 df	株高 Plant height	穗位高 Ear height	雄穗分支数 Tassel branch No.	穗长 Ear length	秃尖长 Barren ear tip length	穗粗 Ear diameter	穗行数 Ear row No.	行粒数 Kernels per row	百粒重 100-kernel weight	出籽率 Seed-producing percentage	产量 Yield
自交系间 Inbred line	12	26 496.22^**	10 414.84^**	446.90^**	88.81^**	3.02^**	3.59^**	73.26^**	313.36^**	63.69^**	1 052.19^**	6 317.38^**

误差 Error	355	206.44	100.50	4.41	1.59	0.32	0.06	2.10	12.24	3.82	16.13	300.45

名称 Name	株高 Plant height/cm	穗位高 Earheight/ cm	雄穗分支数 Tassel branch number	穗长 Ear length/cm	秃尖长 Barren ear tip length/cm	穗粗 Ear diameter/cm	穗行数 Ear row number	行粒数 Kernels per row	百粒重 100-kernel weight/g	出籽率 Seed-producing percentage/%	产量 Yield/(g· plant^-1)
K115	212.03± 1.93 c	100.67± 1.66 a	13.63± 0.39 a	14.57± 0.20 cd	0.32± 0.07 bcde	3.67± 0.03 e	14.87± 0.21 e	22.67± 0.52 de	21.03± 1.50 e	70.63± 0.36 e	70.96± 1.99 e
K492	231.50± 2.24 b	67.50± 1.98 c	2.07± 0.14 h	12.70± 0.118 h	0.86± 0.14 ab	3.60± 0.04 e	12.40± 0.18 g	27.50± 0.97 ab	15.38± 0.10 f	76.30± 0.79 d	52.13± 1.96 f
K131	198.88± 4.67 ef	57.75± 4.08 d	4.63± 0.26 ef	15.13± 0.55 bc	1.13± 0.47 ab	4.18± 0.08 d	15.50± 0.50 cde	24.50± 2.22 bc	28.95± 0.15 bc	78.45± 1.24 c	87.90± 5.43 b
K351	209.07± 2.26 cd	53.90± 2.03 d	2.97± 0.13 gh	15.63± 0.37 b	0.56± 0.15 bc	4.34± 0.05 c	15.33± 0.29 cde	24.93± 0.66 bc	32.39± 0.16 a	84.75± 0.57 a	100.93± 3.51 a
K962	250.00± 2.83 a	64.93± 2.26 c	4.00± 0.32 fg	14.82± 0.16 cd	0.75± 0.10 b	4.41± 0.05 bc	15.60± 0.28 cde	30.07± 0.54 a	22.03± 0.30 e	80.77± 0.29 b	85.38± 3.58 bc
K311	245.90± 1.91 a	106.00± 2.48 a	14.30± 0.73 a	17.34± 0.23 a	0.90± 0.09 ab	4.18± 0.03 d	13.93± 0.18 f	30.97± 0.48 a	31.01± 0.49 ab	80.50± 0.29 b	111.26± 3.16 a
K143	241.00± 3.28 ab	99.73± 1.99 a	7.30± 0.43 c	15.57± 0.18 bc	0.93± 0.18 ab	4.33± 0.05 c	15.79± 0.21 cd	26.34± 0.72 b	23.54± 3.63 e	83.77± 0.60 ab	96.05± 3.76 ab
K324	151.67± 3.03 i	48.10± 1.03 e	5.67± 0.37 de	13.25± 0.19 fgh	0.26± 0.08 cde	4.66± 0.05 ab	15.60± 0.24 cde	24.23± 0.58 bcd	27.19± 0.22 c	82.23± 0.66 b	88.29± 3.07 b
K61	204.07± 2.32 def	58.50± 1.34 d	6.67± 0.50 cd	12.95± 0.35 gh	0.45± 0.12 bcd	4.30± 0.05 cd	16.87± 0.37 b	21.53± 0.92 e	27.16± 0.64 c	70.86± 1.73 e	80.37± 4.79 bcd
K181	198.47± 3.14 ef	68.43± 2.13 c	6.20± 0.29 d	14.30± 0.19 cde	0.10± 0.06 de	4.72± 0.05 a	18.53± 0.27 a	24.80± 0.57 bc	23.84± 0.10 de	83.23± 0.67 ab	88.54± 3.42 b
K322	205.00± 3.60 cde	74.07± 1.80 b	3.23± 0.18 g	13.55± 0.29 fg	0.19± 0.06 cde	4.37± 0.05 c	16.14± 0.30 bc	25.34± 0.64 bc	27.00± 0.35 cd	80.49± 0.68 b	83.95± 2.27 bcd
K111	160.57± 1.62 h	58.20± 0.93 d	6.47± 0.35 cd	10.12± 0.13 i	0.33± 0.06 bcde	4.70± 0.06 a	16.47± 0.32 bc	18.87± 0.42 f	29.45± 0.21 abc	84.28± 0.64 ab	75.18± 2.97 de
K365	197.13± 1.22 fg	69.47± 1.56 bc	10.67± 0.40 b	13.82± 0.16 ef	0± 0 e	4.54± 0.04 b	13.40± 0.27 f	24.37± 0.35 bcd	26.83± 0.18 cd	84.50± 0.38 a	77.82± 2.52 cde

Breeding potential analyses of 13 new inbred lines in maize

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序号 Number	引物 Primer	染色体位置 Chromosome	基因型数 Genotype number	等位基因片段数 Allele number	序号 Number	引物 Primer	染色体位置 Chromosome	基因型数 Genotype number	等位基因片段数 Allele number
1	bnlg439w1^d	1.03	5	4	21	umc1147y4^d	1.07	3	3
2	umc1335y5^c	1.06	4	4	22	bnlg1671y17^a	1.1	9	15
3	umc2007y4^a	2.04	8	8	23	phi96100y1^a	2	5	5
4	bnlg1940k7^c	2.08	4	3	24	umc1536k9^d	2.07	3	3
5	umc2105k3^c	3	5	5	25	bnlg1520K1^a	2.09	7	10
6	phi053k2^d	3.05	3	2	26	umc1489y3^d	3.07	2	2
7	phi072k4^b	4.01	2	2	27	bnlg490y4^d	4.04	5	6
8	bnlg2291k4^b	4.06	4	4	28	umc1999y3^c	4.09	3	3
9	umc1705w1^b	5.03	7	5	29	umc2115k3^b	5.02	5	4
10	bnlg2305k4^d	5.07	7	10	30	umc1429y7^b	5.03	3	3
11	bnlg161k8^b	6	10	10	31	bnlg249k2^b	6.01	3	3
12	bnlg1702k1^b	6.05	5	13	32	phi299852y2^b	6.07	4	3
13	umc1545y2^d	7	2	2	33	umc2160k3^b	7.01	4	7
14	umc1125y3^b	7.04	3	3	34	umc1936k4^c	7.03	2	4
15	bnlg240k1^c	8.06	5	4	35	bnlg2235y5^b	8.02	5	5
16	phi080k15^c	8.08	2	2	36	phi233376y1^c	8.09	4	4
17	phi065k9^d	9.03	2	2	37	umc2084w2^d	9.01	4	4
18	umc1492y13^c	9.04	2	2	38	umc1231k4^a	9.05	6	8
19	umc1432y6^c	10.02	4	3	39	phi041y6^c	10	3	3
20	umc1506k12^a	10.05	4	4	40	umc2163w3^d	10.04	5	5

变异来源 Source of variation	自由度 df	平方和 SS	均方 MS	F
地点Location	1	23 603.4	23 603.4	57.7^**
地点内区组 Block in location	4	4 096.4	1 024.1	2.5^*
组合Combination	209	893 530.8	4 275.3	10.45^**
地点×组合 Location×combination	209	199 610.7	955.1	2.34^**
一般配合力GCA	20	685 575.0	34 278.75	5.51^**
特殊配合力SCA	189	207 951.2	1 100.27	2.36^**
一般配合力×地点 GCA×location	20	111 644.9	5 582.25	11.99^**
特殊配合力×地点 SCA×location	189	87 971.39	465.46	1.14^*
误差 Residual	836	341 938.6	409.0
总计Total	1 259	1 462 780

自交系类别 Classify of inbred line	名称 Name	产量GCA GCA of yield
自交系类别 Classify of inbred line	名称 Name	双流 Shuangliu	宣威 Xuanwei	平均 Average
新自交系	K115	18.59^**	16.01^**	17.30^**
New inbred line	K492	-31.01^**	-29.05^**	-30.03^**
	K131	-4.06	1.24	-1.41
	K351	-8.06^**	-13.27^**	-10.67^**
	K962	4.31	13.09^**	8.70^**
	K311	5.61^*	14.85^**	10.23^**
	K143	27.38^**	33.45^**	30.41^**
	K324	-19.97^**	-21.73^**	-20.85^**
	K61	24.43^**	61.52^**	42.98^**
	K181	-6.93^**	-3.53	-5.23^*
	K322	-2.57	-12.40^**	-7.48^**
	K111	-7.90^**	-15.14^**	-11.52^**
	K365	5.18	11.38^**	8.28^**
骨干自交系	698-3	-4.93	-10.96^**	-7.94^**
Elite inbred line	K169	5.41^*	-20.93^**	-7.76^**
	K236	-5.85^*	11.69^**	2.92
	R08	-1.80	-26.77^**	-14.29^**
	郑58	-23.28^**	-23.08^**	-23.18^**
	K305	11.12^**	5.59^*	8.35^**
	48-2	-2.71	7.61^**	2.45
	21-ES	17.04^**	0.44	8.74^**
LSD_0.05 LSD_0.01	5.24 6.90	5.00 6.58	4.08 5.37

组合来源 Source of combination	新自交系×新自交系 New inbred line×new inbred line		骨干系×骨干系 Elite inbred line×Elite inbred line		骨干系×新自交系 Elite inbred line×New inbred line
组合来源 Source of combination	双流 Shuangliu	宣威 Xuanwei	双流 Shuangliu	宣威 Xuanwei	双流 Shuangliu	宣威 Xuanwei
变幅Range of variation	-48.77~47.73	-55.75~39.42	-35.11~22.90	-33.57~26.00	-41.67~53.96	-49.04~37.26
正效应组合数	39	38	14	12	57	51
Numbers of positive combination
正向显著组合数	6	2	1	1	8	8
Numbers of significantly positive combination
正向显著组合所占比例	2.86	0.95	0.48	0.48	3.81	3.81
Proportion of significantly positive combination
SCA效应值最大组合	K111×K61	K111×K61	K305×R08	K305×698-3	698-3×K351	郑58×K492
Max. combination with SCA						Zheng 58×K492

地点 Location	次位 Rank	组合 Combination	增产量 Yield increase/g	对照优势 Heterosis over CK/%	杂种优势模式 Heterosis pattern
双流	1	K61×K143	61.12	35.12	新类群3×PB New group 3×PB
Shuangliu	2	K143×K115	47.27	31.62	PB×新类群1 PB×new group 1
	3	K111×K61	49.93	30.26	新类群1×新类群3 New group 1×new group 3
	4	21-ES×K143	45.82	29.96	塘四平头×PB Tangsipingtou×PB
	5	21-ES×K61	42.74	28.20	塘四平头×新类群3 Tangsipingtou×new group 3
	6	K181×K115	41.00	26.14	旅大红骨×新类群1 Lyudahonggu×new group 1
	7	K365×K143	36.21	25.29	新类群1×PB New group 1×PB
	8	K305×K169	39.03	25.09	旅大红骨×新类群1 Lyudahonggu×new group 1
	9	K365×K115	31.68	21.37	新类群1×新类群1 New group 1×new group 1
	10	21-ES×K305	29.44	19.43	塘四平头×旅大红骨 Tangsipingtou×Lyudahonggu
	11	K322×K61	30.67	19.39	新类群2×新类群3 New group 2×new group 3
	12	698-3×K143	29.05	17.77	兰卡斯特×PB Lancaster×PB
	13	K305×K143	29.08	17.43	旅大红骨×PB Lyudahonggu×PB
	14	21-ES×K365	26.53	17.16	塘四平头×新类群1 Tangsipingtou×new group 1
	15	郑58×K115 Zheng58×K115	26.27	16.52	瑞德×新类群1 Reid×new group 1
	16	K143×K131	26.03	16.19	PB×新类群2 PB×new group 2
	17	R08×K143	26.35	15.81	新类群1×PB New group 1×PB
	18	K61×K115	23.27	15.62	新类群3×PB New group 3×PB
宣威	1	K61×K143	55.10	31.19	新类群3×PB New group 3×PB
Xuanwei	2	K61×K311	53.29	26.83	新类群3×新类群3 New group 3×new group 3
	3	21-ES×K61	34.99	17.41	塘四平头×新类群3 Tangsipingtou×new group 3

[1]	YANG Mei, HU Xiaolan, SHEN Tao, TAN Kang, LIU Dailing, QIU Hongbo. Construction of single fragment substitution lines of maize 8th chromosome and sreening of resistant maize germplasm to gray leaf spot [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 383-389.
[2]	QU Zhan, YANG Litao. Development of plasmid DNA reference material of genetically modified maize TC1507 [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 390-395.
[3]	GOU Bingdiao, DUAN Panpan, YANG Nan, ZHAO Shufang, WANG Yongfu, ZHANG Gaoyuan, WEI Bingqiang. Heterosis analysis of photosynthetic parameters of pepper seedling responding to low temperature and low light stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 429-436.
[4]	LIU Genhong, XUE Yinxin, ZHANG Qian, ZHOU Jiarui, MAI Xiaofeng. Effects of different tillage depth and amount of straw returned to the field on maize growth under drip-irrigation [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 8-17.
[5]	HONG Xia, ZHAO Yongbin, QU Weidong, CHEN Yinlong, QIU Liping, WANG Jiaoyang. Comparative analysis on genetic diversity of Colocasia esculenta germplasm in Zhejiang Province based on phenotype and simple sequence repeats markers [J]. , 2020, 32(9): 1544-1554.
[6]	CHANG Huiqing, XU Fujin, PAN Yajie. Passivation effects of calcium carbonate with/without chitosan on chromium pollution in calcareous soil [J]. , 2020, 32(9): 1665-1671.
[7]	GUO Yanjing, XIAO Haifeng. Support level and structural characteristics of maize subsidy policy in world's major maize producing countries and region [J]. , 2020, 32(9): 1722-1731.
[8]	YAN Fulin, WANG Bo, WEN Di, XU Wenfen, SUN Qingwen, WEI Shenghua. Genetic diversity analysis of Sabia parviflora based on chloroplast gene psbA-trnH sequence [J]. , 2020, 32(5): 810-815.
[9]	GUO Qinwei, ZHANG Ting, LIU Huiqin, ZHANG Xinhui, LI Chaosen, XIANG Xiaomin, ZHAO Dongfeng, WAN Hongjian. Evaluation of genetic diversity in Luffa germplasm resources in China based on ISSR molecular marker [J]. , 2020, 32(4): 616-623.
[10]	HU Qianwen, XU Yanhao, WANG Rong, ZHANG Wenying, HUA Wei, LYU Chao. Association analysis of four spike traits in barley [J]. , 2020, 32(11): 1941-1953.
[11]	JIANG Yuanyuan, JI Yi, LAI Yongmin, CHEN Xiaoyun, XU Junfeng, XU Xiaoli, MA Lianju. Safety evaluation of Cry-transgenic insect-resistant maize on silkworm, Bombyx mori [J]. , 2020, 32(11): 2042-2049.
[12]	ZHONG Jing, TAN Fen, ZHANG Hongquan, XIONG Xiaoqin, HUANG Lixia. Expression pattern and protein structure analysis of maize XYLPs gene family [J]. , 2020, 32(10): 1741-1747.
[13]	YIN Xiaoxiao, LI Yanfang, GU Jiang, LIAO Yan, XIE Yue, YANG Guangyou, GU Xiaobin. Genetic diversity analysis of Psoroptes ovis var. cuniculi by full-length of mitochondrial ATP6 gene in China [J]. , 2019, 31(8): 1231-1238.
[14]	TANG Shoujie, BI Xiang, ZHANG Feiming, ZHANG Youliang. Genetic diversity analysis and RAPD identification of three successive generations of meio-gynogenetic population in Megalobrama amblycephala [J]. , 2019, 31(8): 1257-1271.
[15]	YUE Gaohong, PAN Binrong, LIU Yongan, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. Genetic diversity analysis of sweet maize inbred lines in Southern Zhejiang by SSR markers [J]. , 2019, 31(7): 1029-1036.