Transcriptome analysis on development of aerial root in grape of Weike

doi:10.3969/j.issn.1004-1524.2020.09.13

›› 2020, Vol. 32 ›› Issue (9): 1645-1655.DOI: 10.3969/j.issn.1004-1524.2020.09.13

• Horticultural Science • Previous Articles Next Articles

Transcriptome analysis on development of aerial root in grape of Weike

GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman^*

Lianyungang Academy of Agricultural Sciences, Lianyungang 222000, China

Received:2020-03-13 Online:2020-09-25 Published:2020-10-10

Abstract

Abstract: Aerial root is a special type of root in grape which grows under high temperature and high humidity. The aerial root has low developmental degree and high potential for differentiation. In this study, techniques were used to induce aerial germination and rooting at the stem nodes of the fruiting branch of Weike grape, and the expression of root transcription genes was analyzed with the untreated stem nodes of the same mother branch as the control. The results showed that the expression of 3 989 genes was increased and the expression of 2 830 genes was down-regulated during the induction of aerial roots. GO functional classification was used to classify annotated genes into 40 functional categories, including catalytic activity, binding, and metabolic processes. Parts of the root key gene expression results showed that all plant hormones including auxin, gibberellin and abscisic acid were involved in the regulation process. Combined with corresponding gene analysis of auxin downstream, endoglucanase, PRE gene and high-affinity nitrate transporter gene significantly up-regulated in the aerial root development, indicating that the degradation of epidermal cell wall at stem nodes and the enrichment of nitrate at stem nodes to stimulate root germination were important regulatory mechanisms for the growth and development of aerial roots.

Key words: grape, aerial root, root system, transcriptome sequencing, auxin

CLC Number:

S663.1

GE Jintao, WANG Jiangying, ZHAO Wenjing, SHAO Xiaobin, ZHU Pengbo, TANG Xueyan, SUN Mingwei, LIU Xingman. Transcriptome analysis on development of aerial root in grape of Weike[J]. , 2020, 32(9): 1645-1655.

References

[1] DUAN L N, DIETRICH D, NG C H, et al.Endodermal ABA signaling promotes lateral root quiescence during salt stress in Arabidopsis seedlings[J]. The Plant Cell, 2013, 25(1):324-341.
[2] ROYCHOUDHRY S, DEL BIANCO M, KIEFFER M, et al.Auxin controls gravitropic setpoint angle in higher plant lateral branches[J]. Current Biology, 2013, 23(15):1497-1504.
[3] QUE F, WANG G L, FENG K, et al.Hypoxia enhances lignification and affects the anatomical structure in hydroponic cultivation of carrot taproot[J]. Plant Cell Reports, 2018, 37(7):1021-1032.
[4] DEB C R, PONGENER A.Studies on the in vitro regenerative competence of aerial roots of two horticultural important Cymbidium species[J]. Journal of Plant Biochemistry and Biotechnology, 2012, 21(2):235-241.
[5] 刘铃, 武小龙, 诸葛强. 植物应答非生物胁迫信号传导研究进展[J]. 分子植物育种, 2018, 16(2):614-625.
LIU L, WU X L, ZHUGE Q.Research progress of abiotic stress responsive signal pathway in plant[J]. Molecular Plant Breeding, 2018, 16(2):614-625.(in Chinese with English abstract)
[6] LIN J Y, MAZAREI M, ZHAO N, et al.Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode[J]. Plant Biotechnology Journal, 2013, 11(9):1135-1145.
[7] RIZVI A, KHAN M S.Biotoxic impact of heavy metals on growth, oxidative stress and morphological changes in root structure of wheat (Triticum aestivum L.) and stress alleviation by Pseudomonas aeruginosa strain CPSB1[J]. Chemosphere, 2017, 185:942-952.
[8] 王会民, 唐秀英, 龙启樟, 等. 水稻根系遗传特征研究进展[J]. 分子植物育种, 2016, 14(4):910-917.
WANG H M, TANG X Y, LONG Q Z, et al.Research progress on genetic characteristics of rice root[J]. Molecular Plant Breeding, 2016, 14(4):910-917.(in Chinese with English abstract)
[9] PACURAR D I, PERRONE I, BELLINI C.Auxin is a central player in the hormone cross-talks that control adventitious rooting[J]. Physiologia Plantarum, 2014, 151(1):83-96.
[10] 朱晓琛, 张汉马, 南文斌. 脱落酸调控植物根系生长发育的研究进展[J]. 植物生理学报, 2017, 53(7):1123-1130.
ZHU X C, ZHANG H M, NAN W B.Research progress on regulation of ABA in plant root development[J]. Plant Physiology Communications, 2017, 53(7):1123-1130.(in Chinese with English abstract)
[11] STAFNE E T, PUCKETTE J A.Aerial root formation on winegrape cultivars after spring 2007 freeze events[J]. American Journal of Enology and Viticulture, 2011, 62(1):118-121.
[12] 葛金涛, 吴秋月, 王金生, 等. 葡萄二次果的诱导及水培盆景培育技术[J]. 中外葡萄与葡萄酒, 2019(4):45-47.
GE J T, WU Q Y, WANG J S, et al.Induction of second fruits of grapevine and cultivation of potted landscape by hydroponic culture[J]. Sino-Overseas Grapevine & Wine, 2019(4):45-47.(in Chinese with English abstract)
[13] PERTEA M, PERTEA G M, ANTONESCU C M, et al.StringTie enables improved reconstruction of a transcriptome from RNA-seq reads[J]. Nature Biotechnology, 2015, 33(3):290-295.
[14] XIE X D, QIN G Y, SI P, et al.Analysis of Nicotiana tabacum PIN genes identifies NtPIN₄ as a key regulator of axillary bud growth[J]. Physiologia Plantarum, 2017, 160(2):222-239.
[15] 司马晓娇, 郑炳松. 植物生长素原初响应基因Aux/IAA研究进展[J]. 浙江农林大学学报, 2015, 32(2):313-318.
SIMA X J, ZHENG B S.Advances in primary auxin-responsive Aux/IAA gene family: a review[J]. Journal of Zhejiang A&F University, 2015, 32(2):313-318.(in Chinese with English abstract)
[16] 袁华招, 赵密珍, 吴伟民, 等. 葡萄生长素响应基因家族生物信息学鉴定和表达分析[J]. 遗传, 2015, 37(7):720-730.
YUAN H Z, ZHAO M Z, WU W M, et al.Genome-wide identification and expression analysis of auxin-related gene families in grape[J]. Hereditas, 2015, 37(7):720-730.(in Chinese with English abstract)
[17] 李傲, 崔梦杰, 陈珂, 等. 葡萄SAUR基因家族鉴定与生物信息学分析[J]. 植物遗传资源学报, 2018, 19(2):326-337.
LI A, CUI M J, CHEN K, et al.Identification and bioinformatics analysis of the SAUR gene family in grape[J]. Journal of Plant Genetic Resources, 2018, 19(2):326-337.(in Chinese with English abstract)
[18] 李保珠, 安国勇, 韩栓. 植物激素ABA在水分胁迫下的功能及信号途径[J]. 植物生理学报, 2012, 48(1):11-18.
LI B Z, AN G Y, HAN S.Function and signaling of plant hormone ABA under water stress[J]. Plant Physiology Communications, 2012, 48(1):11-18.(in Chinese with English abstract)
[19] ZHAO F Y, CAI F X, GAO H J, et al.ABA plays essential roles in regulating root growth by interacting with auxin and MAPK signaling pathways and cell-cycle machinery in rice seedlings[J]. Plant Growth Regulation, 2015, 75(2):535-547.
[20] SATO S, KATO T, KAKEGAWA K, et al.Role of the putative membrane-bound endo-1, 4-beta-glucanase KORRIGAN in cell elongation and cellulose synthesis in Arabidopsis thaliana[J]. Plant & Cell Physiology, 2001, 42(3):251-263.
[21] 张合琼, 张汉马, 梁永书, 等. 植物硝酸盐转运蛋白研究进展[J]. 植物生理学报, 2016, 52(2):141-149.
ZHANG H Q, ZHANG H M, LIANG Y S, et al.Research progress of nitrate in plant transport mechanism[J]. Plant Physiology Communications, 2016, 52(2):141-149.(in Chinese with English abstract)
[22] DOS SANTOS MARASCHIN F, MEMELINK J, OFFRINGA R. Auxin-induced, SCFTIR1-mediated poly-ubiquitination marks AUX/IAA proteins for degradation[J]. The Plant Journal, 2009, 59(1):100-109.
[23] 马俊杰, 郭凤丹, 王兴军, 等. 生长素合成、运输和信号转导调控植物胚胎早期发育[J]. 植物生理学报, 2019, 55(5):547-557.
MA J J, GUO F D, WANG X J, et al.Auxin synthesis, transport and signal transduction regulate early development of plant embryos[J]. Plant Physiology Communications, 2019, 55(5):547-557.(in Chinese with English abstract)
[24] 安建平, 王小非, 刘鑫, 等. 苹果生长素输入载体MdAUX1的基因克隆及在拟南芥和烟草中的遗传转化[J]. 植物研究, 2016, 36(6):902-908.
AN J P, WANG X F, LIU X, et al.Molecular cloning and genetic transformation analysis of an apple auxin influx transporter gene MdAUX1 in Arabidopsis thaliana and tobacco[J]. Bulletin of Botanical Research, 2016, 36(6):902-908.(in Chinese with English abstract)
[25] CASTELAIN M, LE HIR R, BELLINI C.The non-DNA-binding bHLH transcription factor PRE3/bHLH135/ATBS1/TMO7 is involved in the regulation of light signaling pathway in Arabidopsis[J]. Physiologia Plantarum, 2012, 145(3):450-460.

Transcriptome analysis on development of aerial root in grape of Weike

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HUANG Yongming, SONG Fang, WANG Ce, YAO Jinglei, WANG Zhijing, HE Ligang, WU Liming, JIANG Yingchun. Effects of root pruning on growth and expression of related genes in Poncirus trifoliata [J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 270-277.
[2]	CUI Pengfei, WEI Lingzhu, CHENG Jianhui, XIANG Jiang, LI Mingshan, WU Jiang2, . Effects of different rootstocks on growth and fruit quality of Tiangong Cuiyu grape [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 52-61.
[3]	YANG Mingxia, LIAN Hongjuan, WANG Xiaofang, DU Yiyang, DONG Zhigang, JI Wei. Identification and expression analysis of grape MPT gene family [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2173-2185.
[4]	CAO Yuehua, WEI Lingzhu, SHEN Biwei, CHENG Jianhui, XIANG Jiang, WU Jiang. Effects of rootstocks on growth and fruit quality of Xinyu grape [J]. , 2019, 31(6): 908-914.
[5]	HAN Guomin, LIU Xi, TANG Meiling, DAI Lingmin. Effects of exogenous melatonin on physiological characteristics of 5BB grape leaves under NaCl stress [J]. , 2019, 31(4): 556-564.
[6]	CHEN Hongqiang, XIA Hui, WANG Jin, DENG Qunxian, LIANG Dong, LYU Xiulan, TANG Liping. Identification and expression analysis of STS gene family in grape [J]. , 2019, 31(3): 401-407.
[7]	ZHENG Ting, ZHANG Kekun, ZHU Xudong, GUAN Le, SHANGGUAN Lingfei, JIA Haifeng, FANG Jinggui. Rules of sap flow in grapevine during bleeding period [J]. , 2019, 31(2): 250-259.
[8]	SHANG Jiayin, LI Kai, WANG Chaoxia, JI Xian, SUN Jianjun, WANG Dan, SU Hong, TIAN Shufen. Effects of leaf-to-fruit ratio on pruning efficiency and leaf physiological characteristics of Kyoho grape [J]. , 2019, 31(11): 1855-1862.
[9]	ZHANG Anshi, PANG Jincheng, LIU Ying, GAO Dengtao, WEI Zhifeng. Genetic diversity analysis and fingerprinting construction of Muscat Hamburg grapevine series by iPBS marker [J]. , 2019, 31(10): 1624-1631.
[10]	SHANG Jiayin, LI Kai, WANG Chaoxia, SUN Jianjun, JI Xian, WANG Dan, SU Hong, ZHANG Xiaolei, TIAN Shufen. Effects of summer pruning on fruit quality and 6 kinds of aromatic compounds of Jumeigui grape [J]. , 2019, 31(10): 1639-1646.
[11]	YANG Zhou, LYU Ke, LYU Shan, WANG Junjie, ZHANG Di. Cloning and sequence analysis of two ARF genes and two Aux/IAA genes in Agapanthus praecox ssp. orientalis [J]. , 2019, 31(1): 86-97.
[12]	ZHANG Linzhong, CAI Xuezhen, FANG Congbing. Application of NIR spectroscopy for nondestructive qualitative and quantitative analysis of table grapes berries [J]. , 2018, 30(2): 330-338.
[13]	CHEN Tianyu, GAO Xiaoyang, WU Xiang, WU Jiling, YAN Renzhe, LI Hongling. Dynamic simulation model for branch growth of Red Globe grape [J]. , 2017, 29(7): 1208-1215.
[14]	ZHANG Hao, ZHANG Yiwen, LING Shuping, ZHANG Liang, CHEN Ruoxia, WU Yinliang, ZHU Yong. Effects and risk assessment of imazalil treatment on grape storage [J]. , 2017, 29(5): 840-844.
[15]	YANG Min, HUANG Qiao, CHEN Liang-biao. Construction of type Ⅲ antifreeze protein eukaryotic expression plasmid and expression in zebrafish cell line [J]. , 2016, 28(11): 1862-1866.