Identification and expression analysis of grape MPT gene family

doi:10.3969/j.issn.1004-1524.2020.12.08

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2173-2185.DOI: 10.3969/j.issn.1004-1524.2020.12.08

• Horticultural Science • Previous Articles Next Articles

Identification and expression analysis of grape MPT gene family

YANG Mingxia¹(), LIAN Hongjuan², WANG Xiaofang², DU Yiyang², DONG Zhigang¹, JI Wei¹^,²^,^*()

1. Fruit Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
2. College of Horticulture, Shanxi Agricultural University, Taigu 030801, China

Received:2020-07-05 Online:2020-12-25 Published:2020-12-25
Contact: JI Wei

Abstract

Abstract:

The mitochondrial phosphate transporter (MPT) gene family is an important functional protein located on the innner membrane of the nitochondria, transferring important metabolic substrate inorganic phosphate from the cytoplasm to the mitochondrial matrix through mitochondrial inner membrane and playing a key physiological role in maintaining the normal function of cells. Using bioinformatics tools, the phylogenetic evolution, structure, protein physicochemical properties and conserved motifs of grape MPT gene family members were systematically analyzed, as well as the tissue-specific expression and association of homologous gene. The results showed that sixty-four possible MPT family genes were identified in the whole genome of grapes, which could be divided into 7 groups according to the phylogenetic tree, distributed on 19 chromosomes, and two pairs of genes had a tandem replication phenomenon. Conservative motif analysis showed that all MPT family members contained the conserved domain of Mito_carr. Tissue-specific expression showed that VvMPT gene was highly expressed in peels, flowers and leaves. Homologous gene analysis showed that the VvMPT gene family of grapes might play an important role in the development of floral organs and maintenance of ion concentration.

Key words: grape(Vitis vinifera), mitochondrial phosphate transporter, VvMPT, evolutionary analysis

CLC Number:

S663.1

YANG Mingxia, LIAN Hongjuan, WANG Xiaofang, DU Yiyang, DONG Zhigang, JI Wei. Identification and expression analysis of grape MPT gene family[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2173-2185.

Figures/Tables 7

References 21

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基因名称	标签/基因ID	氨基酸数量	蛋白质分子质量	理论等电位	亲疏水性均值	亚细胞定位
Gene name Locus	Tag/Gene ID	Number of amino acids	Molecular weight/u	pI	Gravy value	Subcellular localization
VvMPT1	VIT_200s0188g00090.1	105	11 459.32	5.59	0.275	C,M
VvMPT2	VIT_200s0407g00020.1	141	15 190.80	9.72	0.173	C,M
VvMPT3	VIT_200s0665g00020.1	413	44 991.26	9.46	0.051	E.R,C
VvMPT4	VIT_200s2349g00010.1	318	33 765.64	10.11	0.189	C
VvMPT5	VIT_201s0011g03920.1	373	40 540.30	9.58	-0.176	N,M
VvMPT6	VIT_201s0026g02750.1	299	33 067.12	9.59	-0.021	CW,M
VvMPT7	VIT_202s0012g01250.1	342	35 128.71	9.42	0.201	E.R,G
VvMPT8	VIT_202s0025g01180.1	354	38 329.57	9.57	0.036	C,M
VvMPT9	VIT_202s0025g03490.2	719	78 933.91	7.25	-0.177	N,C
VvMPT10	VIT_203s0038g00080.1	185	20 472.62	9.27	0.006	M,C
VvMPT11	VIT_203s0038g03950.1	308	33 940.03	8.84	0.094	C,N
VvMPT12	VIT_204s0008g01060.1	344	37 582.55	9.80	0.011	C,M
VvMPT13	VIT_204s0008g01130.1	323	35 939.51	9.55	-0.099	C,M
VvMPT14	VIT_204s0008g01660.1	342	36 678.19	9.39	0.091	C,M
VvMPT15	VIT_204s0008g05240.1	397	42 391.10	9.21	0.036	C,N
VvMPT16	VIT_204s0023g02600.2	313	35 252.75	9.89	-0.106	C,N
VvMPT17	VIT_205s0020g00480.6	341	37 013.85	9.13	0.094	C,N
VvMPT18	VIT_205s0020g01380.2	330	36 404.11	9.24	0.051	C,M
VvMPT19	VIT_205s0020g02900.1	319	35 086.72	9.58	0.017	C,N
VvMPT20	VIT_206s0004g00470.1	299	32 052.55	9.42	0.144	C
VvMPT21	VIT_206s0004g01910.1	393	42 838.09	9.78	-0.098	C,N
VvMPT22	VIT_206s0004g05940.1	302	32 709.31	9.33	0.316	E.R,M
VvMPT23	VIT_206s0004g07350.1	327	35 398.67	8.78	0	C,N
VvMPT24	VIT_207s0005g00950.2	315	34 006.36	9.22	0.103	E.R,M
VvMPT25	VIT_207s0031g02290.1	310	34 495.24	9.26	0.209	C,M
VvMPT26	VIT_207s0104g01160.1	280	30 293.58	9.86	0.042	C,N
VvMPT27	VIT_208s0007g01280.1	336	36 441.82	9.73	0.091	C,N
VvMPT28	VIT_208s0007g01700.1	304	32 487.97	9.63	0.157	E.R,G
VvMPT29	VIT_208s0007g02230.1	381	41 521.44	9.58	0.016	C,M
VvMPT30	VIT_208s0007g07270.1	298	31 869.16	9.50	0.117	C,M
VvMPT31	VIT_208s0058g00450.4	288	30 619.30	7.00	0.174	C,M
VvMPT32	VIT_208s0217g00010.1	306	33 291.98	10.04	-0.018	E.R,C
VvMPT33	VIT_209s0002g01610.1	4800	542 048.11	5.34	-0.253	P,E.R
VvMPT34	VIT_209s0002g04500.1	311	33 514.58	9.26	0.015	C,M
VvMPT35 VvMPT36	VIT_209s0054g00790.1 VIT_210s0003g00060.1	356 303	39 258.11 32 860.11	9.19 9.57	0.099 0.032	C,N E.R,M
VvMPT37	VIT_210s0042g01030.1	192	20 716.43	9.22	0.403	C,N
VvMPT38	VIT_210s0116g01120.1	544	60 225.06	9.68	0.061	M,C
VvMPT39	VIT_211s0016g02470.1	341	37 836.02	9.74	-0.038	C,N
VvMPT40	VIT_211s0016g05110.1	400	43 237.11	9.42	-0.101	C,N
VvMPT41	VIT_212s0028g00450.1	377	41 003.29	9.92	0.052	C,N
VvMPT42	VIT_212s0028g03380.3	435	47 424.93	9.84	0.069	C,M
VvMPT43	VIT_213s0019g02440.12	346	37 516.18	9.88	0.040	C,N
VvMPT44	VIT_213s0019g03410.1	342	37 295.55	9.94	-0.006	C,M
VvMPT45	VIT_213s0019g03870.1	829	91 222.66	9.07	-0.225	C,N
VvMPT46	VIT_213s0064g01050.1	477	52 488.17	9.69	-0.200	C,M
VvMPT47	VIT_214s0068g00890.1	489	54 463.56	5.95	-0.127	C,N
VvMPT48	VIT_214s0068g01250.1	64	6 618.57	6.52	0.155	C,M
VvMPT49	VIT_214s0068g01260.1	363	38 690.14	9.21	0.222	E.R,M
VvMPT50	VIT_214s0083g01170.4	334	36 327.05	9.57	0.195	C,M
VvMPT51	VIT_214s0128g00390.1	322	35 092.78	9.73	0.036	C,N
VvMPT52	VIT_215s0046g01290.1	354	38 337.46	9.49	0.046	C,N
VvMPT53	VIT_215s0046g01370.1	405	44 101.62	9.42	-0.047	C
VvMPT54	VIT_216s0050g02360.1	413	44 954.75	9.60	-0.032	C,M
VvMPT55	VIT_217s0000g00030.1	548	60 729.36	5.96	-0.208	C,N
VvMPT56	VIT_217s0000g00400.1	366	39 178.85	9.32	0.247	E.R,G
VvMPT57	VIT_217s0000g04970.1	310	34 472.99	9.87	0.039	M,C
VvMPT58	VIT_217s0000g07290.1	327	35 814.88	8.91	-0.122	C,M
VvMPT59	VIT_218s0001g05620.1	443	48 408.71	8.77	0.067	C,M
VvMPT60	VIT_218s0001g07320.1	323	34 154.83	9.85	0.166	C
VvMPT61	VIT_218s0122g00010.1	355	38 718.47	9.98	0.117	C,M
VvMPT62	VIT_218s0157g00180.1	311	33 341.80	9.48	0.145	C,M
VvMPT63	VIT_218s0166g00130.1	401	42 669.29	9.40	0.155	C,M
VvMPT64	VIT_219s0177g00370.1	64	7 041.23	7.89	-0.013	M,C

基因名称	标签/基因ID	氨基酸数量	蛋白质分子质量	理论等电位	亲疏水性均值	亚细胞定位
Gene name Locus	Tag/Gene ID	Number of amino acids	Molecular weight/u	pI	Gravy value	Subcellular localization
VvMPT1	VIT_200s0188g00090.1	105	11 459.32	5.59	0.275	C,M
VvMPT2	VIT_200s0407g00020.1	141	15 190.80	9.72	0.173	C,M
VvMPT3	VIT_200s0665g00020.1	413	44 991.26	9.46	0.051	E.R,C
VvMPT4	VIT_200s2349g00010.1	318	33 765.64	10.11	0.189	C
VvMPT5	VIT_201s0011g03920.1	373	40 540.30	9.58	-0.176	N,M
VvMPT6	VIT_201s0026g02750.1	299	33 067.12	9.59	-0.021	CW,M
VvMPT7	VIT_202s0012g01250.1	342	35 128.71	9.42	0.201	E.R,G
VvMPT8	VIT_202s0025g01180.1	354	38 329.57	9.57	0.036	C,M
VvMPT9	VIT_202s0025g03490.2	719	78 933.91	7.25	-0.177	N,C
VvMPT10	VIT_203s0038g00080.1	185	20 472.62	9.27	0.006	M,C
VvMPT11	VIT_203s0038g03950.1	308	33 940.03	8.84	0.094	C,N
VvMPT12	VIT_204s0008g01060.1	344	37 582.55	9.80	0.011	C,M
VvMPT13	VIT_204s0008g01130.1	323	35 939.51	9.55	-0.099	C,M
VvMPT14	VIT_204s0008g01660.1	342	36 678.19	9.39	0.091	C,M
VvMPT15	VIT_204s0008g05240.1	397	42 391.10	9.21	0.036	C,N
VvMPT16	VIT_204s0023g02600.2	313	35 252.75	9.89	-0.106	C,N
VvMPT17	VIT_205s0020g00480.6	341	37 013.85	9.13	0.094	C,N
VvMPT18	VIT_205s0020g01380.2	330	36 404.11	9.24	0.051	C,M
VvMPT19	VIT_205s0020g02900.1	319	35 086.72	9.58	0.017	C,N
VvMPT20	VIT_206s0004g00470.1	299	32 052.55	9.42	0.144	C
VvMPT21	VIT_206s0004g01910.1	393	42 838.09	9.78	-0.098	C,N
VvMPT22	VIT_206s0004g05940.1	302	32 709.31	9.33	0.316	E.R,M
VvMPT23	VIT_206s0004g07350.1	327	35 398.67	8.78	0	C,N
VvMPT24	VIT_207s0005g00950.2	315	34 006.36	9.22	0.103	E.R,M
VvMPT25	VIT_207s0031g02290.1	310	34 495.24	9.26	0.209	C,M
VvMPT26	VIT_207s0104g01160.1	280	30 293.58	9.86	0.042	C,N
VvMPT27	VIT_208s0007g01280.1	336	36 441.82	9.73	0.091	C,N
VvMPT28	VIT_208s0007g01700.1	304	32 487.97	9.63	0.157	E.R,G
VvMPT29	VIT_208s0007g02230.1	381	41 521.44	9.58	0.016	C,M
VvMPT30	VIT_208s0007g07270.1	298	31 869.16	9.50	0.117	C,M
VvMPT31	VIT_208s0058g00450.4	288	30 619.30	7.00	0.174	C,M
VvMPT32	VIT_208s0217g00010.1	306	33 291.98	10.04	-0.018	E.R,C
VvMPT33	VIT_209s0002g01610.1	4800	542 048.11	5.34	-0.253	P,E.R
VvMPT34	VIT_209s0002g04500.1	311	33 514.58	9.26	0.015	C,M
VvMPT35 VvMPT36	VIT_209s0054g00790.1 VIT_210s0003g00060.1	356 303	39 258.11 32 860.11	9.19 9.57	0.099 0.032	C,N E.R,M
VvMPT37	VIT_210s0042g01030.1	192	20 716.43	9.22	0.403	C,N
VvMPT38	VIT_210s0116g01120.1	544	60 225.06	9.68	0.061	M,C
VvMPT39	VIT_211s0016g02470.1	341	37 836.02	9.74	-0.038	C,N
VvMPT40	VIT_211s0016g05110.1	400	43 237.11	9.42	-0.101	C,N
VvMPT41	VIT_212s0028g00450.1	377	41 003.29	9.92	0.052	C,N
VvMPT42	VIT_212s0028g03380.3	435	47 424.93	9.84	0.069	C,M
VvMPT43	VIT_213s0019g02440.12	346	37 516.18	9.88	0.040	C,N
VvMPT44	VIT_213s0019g03410.1	342	37 295.55	9.94	-0.006	C,M
VvMPT45	VIT_213s0019g03870.1	829	91 222.66	9.07	-0.225	C,N
VvMPT46	VIT_213s0064g01050.1	477	52 488.17	9.69	-0.200	C,M
VvMPT47	VIT_214s0068g00890.1	489	54 463.56	5.95	-0.127	C,N
VvMPT48	VIT_214s0068g01250.1	64	6 618.57	6.52	0.155	C,M
VvMPT49	VIT_214s0068g01260.1	363	38 690.14	9.21	0.222	E.R,M
VvMPT50	VIT_214s0083g01170.4	334	36 327.05	9.57	0.195	C,M
VvMPT51	VIT_214s0128g00390.1	322	35 092.78	9.73	0.036	C,N
VvMPT52	VIT_215s0046g01290.1	354	38 337.46	9.49	0.046	C,N
VvMPT53	VIT_215s0046g01370.1	405	44 101.62	9.42	-0.047	C
VvMPT54	VIT_216s0050g02360.1	413	44 954.75	9.60	-0.032	C,M
VvMPT55	VIT_217s0000g00030.1	548	60 729.36	5.96	-0.208	C,N
VvMPT56	VIT_217s0000g00400.1	366	39 178.85	9.32	0.247	E.R,G
VvMPT57	VIT_217s0000g04970.1	310	34 472.99	9.87	0.039	M,C
VvMPT58	VIT_217s0000g07290.1	327	35 814.88	8.91	-0.122	C,M
VvMPT59	VIT_218s0001g05620.1	443	48 408.71	8.77	0.067	C,M
VvMPT60	VIT_218s0001g07320.1	323	34 154.83	9.85	0.166	C
VvMPT61	VIT_218s0122g00010.1	355	38 718.47	9.98	0.117	C,M
VvMPT62	VIT_218s0157g00180.1	311	33 341.80	9.48	0.145	C,M
VvMPT63	VIT_218s0166g00130.1	401	42 669.29	9.40	0.155	C,M
VvMPT64	VIT_219s0177g00370.1	64	7 041.23	7.89	-0.013	M,C

基因名称 Gene name	ABRE	ARE	CGTCA-motif	P-box	TCA	CAT-box	MBS	AuxRR-core	TC-rich repeats	LTR
VvMPT1	0	3	2	1	0	0	0	1	1	1
VvMPT2	1	0	0	0	1	0	1	3	0	1
VvMPT3	1	3	0	1	0	0	1	0	0	0
VvMPT4	5	1	0	1	0	0	0	1	0	0
VvMPT5	2	2	4	0	0	0	0	1	0	0
VvMPT6	1	1	0	2	0	0	0	0	1	0
VvMPT7	3	1	0	1	0	0	0	0	1	0
VvMPT8	1	3	0	0	0	0	0	0	0	1
VvMPT9	2	2	0	1	0	0	0	0	1	2
VvMPT10	8	3	4	0	2	0	0	0	0	0
VvMPT11	4	1	0	1	1	0	0	0	0	0
VvMPT12	1	1	0	1	2	1	0	1	0	0
VvMPT13	0	0	0	1	0	0	0	0	0	0
VvMPT14	0	2	2	0	0	0	0	0	1	0
VvMPT15	1	2	2	0	3	0	0	2	1	0
VvMPT16	3	0	6	1	1	3	0	0	1	1
VvMPT17	1	0	0	4	0	0	1	0	1	0
VvMPT18	1	1	0	0	0	0	0	1	1	0
VvMPT19	1	2	4	0	0	0	1	1	0	0
VvMPT20	5	1	4	0	1	0	0	0	0	0
VvMPT21	1	1	2	0	0	2	0	0	0	0
VvMPT22	3	2	0	0	1	0	0	0	1	0
VvMPT23	1	0	2	1	1	1	0	1	0	0
VvMPT24	1	1	6	1	1	1	0	0	0	0
VvMPT25	0	2	0	3	1	0	1	0	0	0
VvMPT26	0	0	2	1	0	2	1	1	0	0
VvMPT27	1	2	4	0	0	0	4	1	0	0
VvMPT28	2	0	0	0	0	0	0	0	0	1
VvMPT29	0	2	2	1	1	1	0	0	0	0
VvMPT30	2	1	6	0	1	0	0	0	0	1
VvMPT31	0	2	2	1	1	0	0	0	0	1
VvMPT32	2	2	0	3	1	0	1	1	0	0
VvMPT33	0	3	4	1	0	0	1	0	1	0
VvMPT34	3	0	2	2	0	0	0	2	2	0
VvMPT35	3	6	2	2	1	0	2	0	0	0
VvMPT36	0	3	4	1	1	0	1	0	0	1
VvMPT37	0	0	4	0	1	2	0	0	0	2
VvMPT38	3	1	0	2	0	0	1	0	0	0
VvMPT39	5	1	0	0	0	0	0	0	0	1
VvMPT40	0	1	0	1	1	0	0	1	1	0
VvMPT41	6	1	4	1	0	1	0	0	0	0
VvMPT42	1	1	0	0	0	0	0	1	1	0
VvMPT43	2	9	2	1	0	1	0	0	2	3
VvMPT44	1	9	4	1	1	2	0	0	1	0
VvMPT45	0	1	0	1	1	0	2	1	0	0
VvMPT46	3	0	2	1	0	0	0	1	1	1
VvMPT47	1	3	0	1	0	0	1	0	1	0
VvMPT48	4	3	2	1	1	1	1	2	0	1
VvMPT49	0	5	2	0	1	0	2	0	0	1
VvMPT50	2	1	4	0	0	2	0	0	0	0
VvMPT51	9	2	6	0	0	1	1	1	1	0
VvMPT52	3	3	2	0	1	0	0	0	2	0
VvMPT53	3	2	0	1	0	0	0	0	0	1
VvMPT54	1	1	2	1	0	3	2	1	0	0
VvMPT55	0	2	0	0	1	1	2	0	0	0
VvMPT56	8	0	4	0	2	1	1	2	0	0
VvMPT57	3	1	0	2	0	2	0	0	0	1
VvMPT58	0	2	0	1	0	1	0	0	3	1
VvMPT59	8	2	2	0	1	0	1	0	0	0
VvMPT60	3	1	8	0	0	2	1	0	0	0
VvMPT61	0	4	0	3	1	0	2	0	0	0
VvMPT62	0	0	0	0	0	0	0	0	0	0
VvMPT63	0	0	2	3	1	0	0	0	1	0
VvMPT64	0	1	4	3	0	0	0	0	0	0
总计Total	125	115	122	57	37	34	32	31	28	22

基因名称 Gene name	ABRE	ARE	CGTCA-motif	P-box	TCA	CAT-box	MBS	AuxRR-core	TC-rich repeats	LTR
VvMPT1	0	3	2	1	0	0	0	1	1	1
VvMPT2	1	0	0	0	1	0	1	3	0	1
VvMPT3	1	3	0	1	0	0	1	0	0	0
VvMPT4	5	1	0	1	0	0	0	1	0	0
VvMPT5	2	2	4	0	0	0	0	1	0	0
VvMPT6	1	1	0	2	0	0	0	0	1	0
VvMPT7	3	1	0	1	0	0	0	0	1	0
VvMPT8	1	3	0	0	0	0	0	0	0	1
VvMPT9	2	2	0	1	0	0	0	0	1	2
VvMPT10	8	3	4	0	2	0	0	0	0	0
VvMPT11	4	1	0	1	1	0	0	0	0	0
VvMPT12	1	1	0	1	2	1	0	1	0	0
VvMPT13	0	0	0	1	0	0	0	0	0	0
VvMPT14	0	2	2	0	0	0	0	0	1	0
VvMPT15	1	2	2	0	3	0	0	2	1	0
VvMPT16	3	0	6	1	1	3	0	0	1	1
VvMPT17	1	0	0	4	0	0	1	0	1	0
VvMPT18	1	1	0	0	0	0	0	1	1	0
VvMPT19	1	2	4	0	0	0	1	1	0	0
VvMPT20	5	1	4	0	1	0	0	0	0	0
VvMPT21	1	1	2	0	0	2	0	0	0	0
VvMPT22	3	2	0	0	1	0	0	0	1	0
VvMPT23	1	0	2	1	1	1	0	1	0	0
VvMPT24	1	1	6	1	1	1	0	0	0	0
VvMPT25	0	2	0	3	1	0	1	0	0	0
VvMPT26	0	0	2	1	0	2	1	1	0	0
VvMPT27	1	2	4	0	0	0	4	1	0	0
VvMPT28	2	0	0	0	0	0	0	0	0	1
VvMPT29	0	2	2	1	1	1	0	0	0	0
VvMPT30	2	1	6	0	1	0	0	0	0	1
VvMPT31	0	2	2	1	1	0	0	0	0	1
VvMPT32	2	2	0	3	1	0	1	1	0	0
VvMPT33	0	3	4	1	0	0	1	0	1	0
VvMPT34	3	0	2	2	0	0	0	2	2	0
VvMPT35	3	6	2	2	1	0	2	0	0	0
VvMPT36	0	3	4	1	1	0	1	0	0	1
VvMPT37	0	0	4	0	1	2	0	0	0	2
VvMPT38	3	1	0	2	0	0	1	0	0	0
VvMPT39	5	1	0	0	0	0	0	0	0	1
VvMPT40	0	1	0	1	1	0	0	1	1	0
VvMPT41	6	1	4	1	0	1	0	0	0	0
VvMPT42	1	1	0	0	0	0	0	1	1	0
VvMPT43	2	9	2	1	0	1	0	0	2	3
VvMPT44	1	9	4	1	1	2	0	0	1	0
VvMPT45	0	1	0	1	1	0	2	1	0	0
VvMPT46	3	0	2	1	0	0	0	1	1	1
VvMPT47	1	3	0	1	0	0	1	0	1	0
VvMPT48	4	3	2	1	1	1	1	2	0	1
VvMPT49	0	5	2	0	1	0	2	0	0	1
VvMPT50	2	1	4	0	0	2	0	0	0	0
VvMPT51	9	2	6	0	0	1	1	1	1	0
VvMPT52	3	3	2	0	1	0	0	0	2	0
VvMPT53	3	2	0	1	0	0	0	0	0	1
VvMPT54	1	1	2	1	0	3	2	1	0	0
VvMPT55	0	2	0	0	1	1	2	0	0	0
VvMPT56	8	0	4	0	2	1	1	2	0	0
VvMPT57	3	1	0	2	0	2	0	0	0	1
VvMPT58	0	2	0	1	0	1	0	0	3	1
VvMPT59	8	2	2	0	1	0	1	0	0	0
VvMPT60	3	1	8	0	0	2	1	0	0	0
VvMPT61	0	4	0	3	1	0	2	0	0	0
VvMPT62	0	0	0	0	0	0	0	0	0	0
VvMPT63	0	0	2	3	1	0	0	0	1	0
VvMPT64	0	1	4	3	0	0	0	0	0	0
总计Total	125	115	122	57	37	34	32	31	28	22

Identification and expression analysis of grape MPT gene family

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