Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers

doi:10.3969/j.issn.1004-1524.2018.02.08

›› 2018, Vol. 30 ›› Issue (2): 236-241.DOI: 10.3969/j.issn.1004-1524.2018.02.08

• Horticultural Science • Previous Articles Next Articles

Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers

WANG Qingjun¹, MA Li², HAO Zhaoxiang¹, LUO Hua¹, BI Runxia¹, ZHAO Li'na¹, HOU Lefeng^{1, *}

1.Zaozhuang Pomegranate Research Center, Zaozhuang 277300,China;
2. Zaozhuang University, Zaozhuang 277160,China

Received:2017-06-30 Online:2018-02-20 Published:2018-02-11

Abstract

Abstract: In order to investigate the germplasm genetic diversity and genetic relationship of ornamental pomegranate (Punica granatum L.), the genetic relationships of 35 ornamental pomegranate cultivars were evaluated by ISSR markers. Eight ISSR primers with good polymorphism were screened out. A total of 168 bands were amplified from 8 ISSR primers, of which 158 bands were polymorphic loci. The percentage of polymorphic band (PPB) was 94.05%, the number of alleles (Na) was 1.940 5, the effective number of alleles (Ne) was 1.678 5, Nei's gene diversity (He) was 0.379 9 and Shannon's information index (I) was 0.553 0. The genetic similarities coefficient (GS) between cultivars ranged from 0.416 7 to 0.922 6, suggesting ornamental pomegranate cultivars had abundant genetic diversity. Thirty-five ornamental pomegranate cultivars were divided into 6 groups at GS of 0.668 based on UPGMA method.

Key words: ornamental pomegranate (Punica granatum L.), genetic diversity, ISSR

CLC Number:

S665.4

WANG Qingjun, MA Li, HAO Zhaoxiang, LUO Hua, BI Runxia, ZHAO Li'na, HOU Lefeng. Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers[J]. , 2018, 30(2): 236-241.

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Analysis on germplasm genetic diversity of ornamental pomegranate (Punica granatum L.) by ISSR markers

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