Antifungal effect of Camellia seed cake on root rot pathogens of Astragalus membranaceus and Panax notoginseng

doi:10.3969/j.issn.1004-1524.2022.06.13

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1227-1235.DOI: 10.3969/j.issn.1004-1524.2022.06.13

• Plant protection • Previous Articles Next Articles

Antifungal effect of Camellia seed cake on root rot pathogens of Astragalus membranaceus and Panax notoginseng

YANG Xiujuan¹(), LI Weiya¹, LI Caimiao¹, CHENG Bijun¹, GAO Fen², ZHAO Jun³

1. School of Public Heath, Shanxi Medical University, Taiyuan 030001, China
2. Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China
3. School of Geography, Nanjing Normal University, Nanjing 210023, China

Received:2021-09-13 Online:2022-06-25 Published:2022-06-30

Abstract

Abstract:

The root rot disease of Astragalus membranaceus and Panax notoginseng are increasingly serious which have restricted their yield and quality. To investigate the effect of water extract and volatile organic compounds of Camellia seed cake on root rot fungal pathogens of A. membranaceus and P. notoginseng, six dominant root rot fungal pathogens were used for testing, respectively. 6 strains of dominant pathogenic fungi from Astragalus membranaceus and Panax notoginseng were cultured by plate inoculation method, and the inhibition rate was calculated by measuring the diameter of colony every day. 2 g∙L^-1 water extract of Camellia seed cake showed significant inhibition on A. membranaceus root rot pathogens Fusarium acuminatum, Fusarium solani and Fusarium oxysporum, and inhibition rates were 52.08%, 20.89% and 41.98%, respectively. 5 g∙L^-1 water extract of Camellia seed cake showed significant inhibition on P. notoginseng root rot pathogens F. solani, F. oxysporum and Ilyonectria sp., and inhibition rates were 25.31 %, 30.51% and 13.33%, respectively. 50 g∙L^-1 volatile organic compounds of Camellia seed cake showed inhibition on P. notoginseng root rot pathogens F. oxysporum, F. solani, and Ilyonectria sp., and inhibition rates were 60.16%, 52.16% and 40.47%, respectively. Tea saponin and five volatile organic compounds of Camellia seed cake showed significant inhibitory effects on the six fungi pathogens, of which 1-octen-3-ol and 2-methylbutyric acid showed the strongest fungistatic ability. This study layed a solid foundation for the effective prevention and control of Chinese herbs root rot disease by Camellia seed cake.

Key words: root rot pathogens, Camellia seed cake, water extract, volatile organic compounds, inhibition rate

CLC Number:

S436.68

YANG Xiujuan, LI Weiya, LI Caimiao, CHENG Bijun, GAO Fen, ZHAO Jun. Antifungal effect of Camellia seed cake on root rot pathogens of Astragalus membranaceus and Panax notoginseng[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1227-1235.

Figures/Tables 5

References 25

[1]	国家药典委员会. 中华人民共和国药典:一部[M].2020年版. 北京:中国医药科技出版社, 2020.
[2]	孙海峰, 康宝玲, 高彦云, 等. 基于ARISA指纹图谱技术的浑源黄芪根际微生物菌群特征剖析[J]. 中草药, 2016, 47(12): 2157-2162.
	SUN H F, KANG B L, GAO Y Y, et al. Characterization of rhizospheric microbial flora of Hunyuan Astragali Mongolici Radix by ARISA fingerprinting[J]. Chinese Traditional and Herbal Drugs, 2016, 47(12): 2157-2162. (in Chinese with English abstract)
[3]	高芬, 赵晓霞, 秦雪梅, 等. 山西省蒙古黄芪根腐病优势致病菌群分析[J]. 植物保护学报, 2018, 45(4): 878-885.
	GAO F, ZHAO X X, QIN X M, et al. Analysis of dominant pathogen community causing Astragalus membranaceus var. mongholicus root rot in Shanxi Province[J]. Journal of Plant Protection, 2018, 45(4): 878-885. (in Chinese with English abstract)
[4]	游春梅, 官会林, 屠文, 等. 三七免耕连作土壤障碍因素及其消减措施的理论思考[J]. 云南师范大学学报(自然科学版), 2010, 30(3): 44-48.
	YOU C M, GUAN H L, TU W, et al. Theoretical thinking about Panax notoginseng's no-tillage cropping soil barriers and mitigation measures[J]. Journal of Yunnan Normal University (Natural Sciences Edition), 2010, 30(3): 44-48. (in Chinese with English abstract)
[5]	赵庆芳, 张艳芳, 李巧峡. 黄芪根腐病病根的显微及超微结构研究[J]. 西北师范大学学报(自然科学版), 2012, 48(5): 84-88.
	ZHAO Q F, ZHANG Y F, LI Q X. Study on the structure of Astragalus membranaceus roots infected by the root rot disease[J]. Journal of Northwest Normal University (Natural Science), 2012, 48(5): 84-88. (in Chinese with English abstract)
[6]	姚春芝, 蒋宇婷, 杨玉婷, 等. 三七连作土壤浸提液对其根腐病菌的化感效应[J]. 应用生态学报, 2020, 31(7): 2227-2235.
	YAO C Z, JIANG Y T, YANG Y T, et al. Allelopathic effect of extracts from Panax notoginseng mono-cropped soil on its root rot pathogens[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2227-2235. (in Chinese with English abstract)
[7]	乔新国. 三七根腐病病原真菌的初步研究[D]. 昆明: 云南大学, 2015.
	QIAO X G. The study of pathogenic fungi with Panax notoginseng root rot[D]. Kunming: Yunnan University, 2015. (in Chinese with English abstract)
[8]	陆宁, 刘云龙, 陈昱君, 等. 引起三七根腐病的两种病原菌[C]// 中国植病学会. 中国菌物学会北海联合年会论文集, 2005: 25-27.
[9]	何晓婷, 王均慧, 李娇, 等. 9种植物提取物对三七根腐病病原菌的抑菌活性测定[J]. 山西农业科学, 2020, 48(5): 789-792.
	HE X T, WANG J H, LI J, et al. Determination of antimicrobial activity of 9 plant extracts against the pathogen of Panax notoginseng root rot[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(5): 789-792. (in Chinese with English abstract)
[10]	王亚萍, 费学谦, 陆宽宽, 等. 油茶籽饼粕中甲醇提取物抑制黄曲霉菌效果及成分分析[J]. 农业工程学报, 2019, 35(11): 322-329.
	WANG Y P, FEI X Q, LU K K, et al. Inhibitory effect of Aspergillus flavus and component analysis of methanol extraction from Camellia seed cake[J]. Transactions of the Chinese Society of Agricultural Engineering, 2019, 35(11): 322-329. (in Chinese with English abstract)
[11]	LI Z, WANG Z C, ZHANG L B, et al. Pharmacological activities of components contained in Camellia oil and Camellia oil cake and their applications in various industries[J]. Current Traditional Medicine, 2020, 6(2): 86-105. DOI URL
[12]	YU X L, HE Y. Tea saponins: effective natural surfactants beneficial for soil remediation, from preparation to application[J]. RSC Advances, 2018, 8(43): 24312-24321. DOI URL
[13]	彭游, 柏杨, 喻国贞, 等. 茶皂素的提取及应用研究新进展[J]. 食品工业科技, 2013, 34(10): 357-362.
	PENG Y, BAI Y, YU G Z, et al. Advances in studies on extraction and application of tea saponin[J]. Science and Technology of Food Industry, 2013, 34(10): 357-362. (in Chinese with English abstract)
[14]	黄继光, 陈秀贤, 徐汉虹, 等. 茶皂素对12种植物病原菌的抑菌活性[J]. 华中农业大学学报, 2013, 32(2): 50-53.
	HUANG J G, CHEN X X, XU H H, et al. Studies on inhibitory activity of tea saponin against twelve plant pathogenic fungi[J]. Journal of Huazhong Agricultural University, 2013, 32(2): 50-53. (in Chinese with English abstract)
[15]	WANG Y P, YANG L, FEI X Q, et al. Antifungal effect of Camellia seed cake extract on Aspergillus flavus[J]. Journal of Food Protection, 2019, 82(3): 463-469. DOI URL
[16]	MENG X C, LI J, BI F C, et al. Antifungal activities of crude extractum from Camellia semiserrata Chi (Nanshancha) seed cake against Colletotrichum musae, Colletotrichum gloeosporioides and Penicillium italicum in vitro and in vivo fruit test[J]. The Plant Pathology Journal, 2015, 31(4): 414-420. DOI URL
[17]	YANG X J, XUE C, SU L X, et al. Exploring patterns of Camellia seed cake application in relation to plant growth, soil Nematodes and microbial biomass[J]. Soil Science and Plant Nutrition, 2018, 64(2): 253-264. DOI URL
[18]	YANG X J, WANG X, WANG K, et al. The nematicidal effect of Camellia seed cake on root-knot nematode Meloidogyne javanica of banana[J]. PLoS One, 2015, 10(4): e0119700. DOI URL
[19]	孟祥春, 黄泽鹏, 凡超, 等. 茶树油粕中茶皂素的提取及其对果蔬采后致病菌的抑制作用[J]. 保鲜与加工, 2019, 19(3): 90-96.
	MENG X C, HUANG Z P, FAN C, et al. Extraction of tea saponin from Camellia semiserrata Chi oil residua and its inhibiting effects on postharvest pathogen of fruit and vegetable[J]. Storage and Process, 2019, 19(3): 90-96. (in Chinese with English abstract)
[20]	王媛媛. 竹叶精油的复配及其抑菌机理研究[D]. 北京: 北京林业大学, 2016.
	WANG Y Y. Antimicrobial mechanism of essential oil from Phyllostachys pubescens leaf and its combination[D]. Beijing: Beijing Forestry University, 2016. (in Chinese with English abstract)
[21]	段一帆. 竹类植物挥发性有机物(BVOCs)抑菌活性及干旱对其释放的影响研究[D]. 雅安: 四川农业大学, 2019.
	DUAN Y F. Study on the antibacterial activity of volatile organic compounds(BVOCs) in bamboo and the effect of drought on their emission[D]. Ya’an: Sichuan Agricultural University, 2019. (in Chinese with English abstract)
[22]	赵亚红, 徐翠霞, 马玲, 等. 3种常绿树挥发物成分对空气负离子及微生物的影响[J]. 浙江农林大学学报, 2020, 37(4): 654-663.
	ZHAO Y H, XU C X, MA L, et al. Effects of volatile components of three evergreen plants on air anion and microorganism[J]. Journal of Zhejiang A & F University, 2020, 37(4): 654-663. (in Chinese with English abstract)
[23]	卫强, 王燕红. 棕榈花、叶、茎挥发油成分及抑菌活性研究[J]. 浙江农业学报, 2016, 28(5): 875-884.
	WEI Q, WANG Y H. Study on chemical components of the essential oils in flower, leaf and stem of Trachycarpus fortune(Hook) H. Wendl. and their antimicrobial activities[J]. Acta Agriculturae Zhejiangensis, 2016, 28(5): 875-884. (in Chinese with English abstract)
[24]	翟永彪. 三种挥发性化合物对山东地区灰霉病菌的毒力及作用方式研究[D]. 泰安: 山东农业大学, 2016.
	ZHAI Y B. Toxicity and mode of action of three kind of volatile compounds against Botrytis cinerea* in Shandong Province*[D]. Tai’an: Shandong Agricultural University, 2016. (in Chinese with English abstract)
[25]	刘元元, 庞学兵, 李国, 等. 棉花黄萎病生防菌的筛选及挥发性抑菌物质检测[J]. 西北农业学报, 2019, 28(5): 820-829.
	LIU Y Y, PANG X B, LI G, et al. Screening for bio-control bacteria against cotton Verticillium wilt and detection of volatile antimicrobial substances[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(5): 820-829. (in Chinese with English abstract)

茶籽饼水浸提液终浓度 Concentrations of Camellia seed cake water extract/(g∙L^-1)	黄芪根腐病病原菌 Root rot pathogens of A. membranaceus			三七根腐病病原菌 Root rot pathogens of P. notoginseng
	F. acuminatum	F. solani	F. oxysporum	F. solani	F. oxysporum	Ilyonectria sp.
2	52.08±4.66 a	20.89±10.29 b	41.98±1.83 a	20.13±7.02 a	8.60±4.07 b	26.67±0.04 a
5	47.69±5.18 a	35.62±4.30 a	46.94±4.06 a	25.31±0.53 a	30.51±4.21 a	13.33±0.01 a
10	28.36±21.44 a	23.69±5.12 a	32.22±5.90 b	4.00±7.62 b	35.60±3.29 a	20.00±0.02 a

茶籽饼水浸提液终浓度 Concentrations of Camellia seed cake water extract/(g∙L^-1)	黄芪根腐病病原菌 Root rot pathogens of A. membranaceus			三七根腐病病原菌 Root rot pathogens of P. notoginseng
	F. acuminatum	F. solani	F. oxysporum	F. solani	F. oxysporum	Ilyonectria sp.
2	52.08±4.66 a	20.89±10.29 b	41.98±1.83 a	20.13±7.02 a	8.60±4.07 b	26.67±0.04 a
5	47.69±5.18 a	35.62±4.30 a	46.94±4.06 a	25.31±0.53 a	30.51±4.21 a	13.33±0.01 a
10	28.36±21.44 a	23.69±5.12 a	32.22±5.90 b	4.00±7.62 b	35.60±3.29 a	20.00±0.02 a

茶籽饼中挥发性气体浓度 Concentrations of volatile gases in Camellia seed cake/(g∙L^-1)	黄芪根腐病病原菌抑制率 Inhibition rate of root rot pathogens of A. membranaceus			三七根腐病病原菌抑制率 Inhibition rate of root rot pathogens of P. notoginseng
	F. acuminatum	F. solani	F. oxysporum	F. solani	F. oxysporum	Ilyonectria sp.
10	22.24±22.92 a	41.79±4.01 a	45.22±1.30 a	45.80±27.97 a	48.72±8.21 a	28.03±8.14 b
50	35.73±9.88 a	51.01±3.15 a	52.36±1.13 a	52.16±6.16 a	60.16±2.28 a	40.47±4.31 a

茶籽饼中挥发性气体浓度 Concentrations of volatile gases in Camellia seed cake/(g∙L^-1)	黄芪根腐病病原菌抑制率 Inhibition rate of root rot pathogens of A. membranaceus			三七根腐病病原菌抑制率 Inhibition rate of root rot pathogens of P. notoginseng
	F. acuminatum	F. solani	F. oxysporum	F. solani	F. oxysporum	Ilyonectria sp.
10	22.24±22.92 a	41.79±4.01 a	45.22±1.30 a	45.80±27.97 a	48.72±8.21 a	28.03±8.14 b
50	35.73±9.88 a	51.01±3.15 a	52.36±1.13 a	52.16±6.16 a	60.16±2.28 a	40.47±4.31 a

药剂Pharmaceutics	添加量 Addition /μL	黄芪根腐病病原菌抑制率Inhibition rate of root rot pathogens of A. membranaceus									三七根腐病病原菌抑制率Inhibition rate of root rot pathogens of P. notoginseng
		F. acuminatum			F. solani			F. oxysporum			F. solani			F. oxysporum			Ilyonectria sp.
		Day2	Day4	Day6	Day2	Day 4	Day6	Day2	Day4	Day6	Day 2	Day4	Day 6	Day 2	Day4	Day 6	Day2	Day 4	Day6
1-辛烯-3-醇 1-Octen-3-ol	10	63.89±4.81 a	73.64±9.68 a	56.61±17.48 b	66.67±0.00 a	42.67±8.33 b	23.42±10.92 b	78.38±0.00 a	75.36±2.05 b	38.37±4.93 b	50.00±0.01 a	60.32±2.75 b	23.53±4.16 b	60.61±5.25 a	51.16±16.44 a	32.35±12.48 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
	50	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	73.33±0.01 a	84.00±0.01 a	89.19±0.01 a	78.38±0.01 a	88.41±0.01 a	90.70±0.01 a	50.00±0.01 a	80.95±0.01 a	88.24±0.01 a	63.64±0.01 a	81.40±0.01 a	88.24±0.01 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
	100	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	73.33±0.01 a	84.00±0.01 a	89.19±0.01 a	78.38±0.01 a	88.41±0.01 a	90.70±0.01 a	50.00±0.01 a	80.95±0.01 a	88.24±0.01 a	63.64±0.01 a	81.40±0.01 a	88.24±0.01 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
2-甲基丁酯 2-Methylbutyl ester	10	11.11±4.81 b	8.53±5.37 b	1.59±3.17 b	8.89±3.85 c	0 b	4.50±1.56 b	15.32±3.12 c	12.08±4.43 c	0 b	0 c	0 c	-2.94±0.01c	12.12±5.25 c	-0.78±5.37 b	-5.88±0.01b	0 b	35.42±3.61 b	5.00±0.01 b
	50	30.56±12.73 b	20.93±8.06 b	8.99±4.85 b	35.56±3.85 b	22.67±4.62 a	18.92±2.70 a	42.34±8.26 b	23.67±6.03 b	1.55±2.69 b	16.67±7.22 b	12.70±2.75 b	6.86±1.70 b	27.27±0.01 b	-2.33±0.01b	-0.98±1.70 b	6.67±11.55 b	39.58±3.61 b	20.00±5.00 a
	100	58.33±14.43 a	47.29±14.95 a	26.98±8.40 a	44.44±3.85 a	25.33±2.31 a	21.62±2.70 a	71.17±8.26 a	48.79±4.43 a	27.13±1.34 a	37.50±0.01 a	38.10±0.01 a	18.63±4.49 a	54.55±0.01 a	37.98±10.74 a	17.65±5.88 a	20.00±0.01 a	52.08±3.61 a	23.33±2.89 a
2-甲基丁酸 2-Methylbutyric	10	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	73.33±0.01 a	84.00±0.01 a	72.97±3.82 b	78.38±0.01 a	88.41±0.01 a	80.62±17.45 a	50.00±0.01 a	80.95±0.01 a	80.88±2.08 b	63.64±0.01 a	81.40±0.01 a	73.53±0.01 a	20.00±0.01a	75.00±0.01 a	80.00±0.01 a
	50	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	73.33±0.01 a	84.00±0.01 a	89.19±0.01 a	78.38±0.01 a	88.41±0.01 a	90.70±0.01 a	50.00±0.01 a	80.95±0.01 a	88.24±0.01 a	63.64±0.01 a	81.40±0.01 a	88.24±0.01 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
	100	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	73.33±0.01 a	84.00± 0.01 a	89.19±0.01 a	78.38±0.01 a	88.41±0.01a	90.70± 0.01 a	50.00±0.01 a	80.95±0.01 a	88.24±0.01 a	63.64±0.01 a	81.40±0.01 a	88.24± 0.01 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
丁酸丁酯 Butyl butyrate	10	22.22±4.81 b	13.18±5.37 b	6.88±1.83 b	20.00±0.01 b	10.67±2.31 b	6.31±1.56 b	63.96±13.60 a	45.89±11.71 b	27.13±7.10 b	0 c	2.38±3.37 b	-0.98±3.40 b	36.36±0.01 c	10.08±2.69 b	0.98±1.70 b	0 a	39.58±7.22 b	11.67±5.77 b
	50	63.89±4.81 a	51.94±14.95 a	30.16±11.00 a	53.33±6.67 a	32.00±4.00 a	23.42±1.56 a	71.17±12.48 a	58.45±12.07 b	38.76±12.81 b	33.33±7.22 b	15.87±7.27 b	12.75±1.70 a	45.45±0.01 b	16.28±0.01 b	10.78±1.70 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
	100	66.67±0.01 a	62.79±4.65 a	43.92±4.85 a	57.78±3.85 a	26.67±2.31 a	19.82±3.12 a	78.38±0.01 a	81.64±6.03 a	61.24±16.33 a	45.83±7.22 a	35.71±16.84 a	16.18±6.24 a	60.61±5.25 a	34.88±12.31 a	15.69±7.40 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a
正丁醇 1-Butanol	10	19.44±12.73 b	17.83±14.21 b	11.11±9.52 c	2.22±3.85 c	8.00±4.00 c	7.21±4.13 b	17.12±6.24 c	19.81±6.69 b	12.40±5.85 c	4.17±7.22 b	1.59±2.75 c	0 c	9.09±0.01 c	8.53±5.37 b	3.92±3.40 b	6.67±11.55 a	54.17±3.61 c	36.67±12.58 c
	50	66.67±0.01 a	70.54±2.69 a	61.90±3.17 b	28.89±10.18 b	25.33±9.24 b	26.13±10.23 b	42.34±3.12 b	38.16±4.43 b	30.23±8.06 b	16.67±7.22 b	20.63±2.75 b	16.67±1.70 b	30.30±5.25 b	17.83±2.69 b	12.75±6.12 b	20.00±0.01 a	66.67±3.61 b	58.33±2.89 b
	100	66.67±0.01 a	81.40±0.01 a	87.30±0.01 a	66.67±6.67 a	48.00±0.01 a	48.65±11.47 a	72.97±9.36 a	71.98±13.39 a	64.34±9.68 a	41.67±7.22 a	50.00±3.37 a	42.65±2.08 a	57.58±5.25 a	67.44±9.30 a	52.94±8.32 a	20.00±0.01 a	75.00±0.01 a	80.00±0.01 a

Antifungal effect of Camellia seed cake on root rot pathogens of Astragalus membranaceus and Panax notoginseng

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 25

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	ZHANG Hongling, LI Sen, ZHANG Yang, SONG Yifan, SHANG Zhaocong. Remediation of perchloroethylene and polycyclic aromatic hydrocarbons contaminated soil by foam-blocking coupled with oxidants [J]. , 2019, 31(7): 1138-1144.
[2]	LAI Yu\\|qing， WANG Mei\\|xian， XIE Ying\\|ran， LIU Yan*. Analysis of volatile organic compounds emitted from Pinus armandii in winter [J]. , 2016, 28(2): 284-.
[3]	LIU Qing\\|e;XIAO Jian\\|zhong;CHEN Hai\\|yan;ZHONG Xian\\|long. Optimization of hot\\|water extraction conditions for polysaccharide in Lentinus edodes waste substrate by quadratic regression rotation\\|orthogonal combination design [J]. , 2013, 25(6): 0-1271.