Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

doi:10.3969/j.issn.1004-1524.2022.06.14

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1236-1247.DOI: 10.3969/j.issn.1004-1524.2022.06.14

• Plant protection • Previous Articles Next Articles

Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

WANG Siliang¹(), SHAO Yue², YAN Chengjin¹

1. College of Agriculture and Biotechnology, Wenzhou Vocational College of Science and Technology, Wenzhou 325000, Zhejiang, China
2. Plant Protection and Soil Fertilizer Management Station of Wenzhou, Wenzhou 325000, Zhejiang, China

Received:2021-05-06 Online:2022-06-25 Published:2022-06-30

Abstract

Abstract:

Spodoptera furgiperda is a major invading pest. In the south of China, S. furgiperda can survive and multiply through whole year. Previous investigations and research revealed the risk that wheat was overwintering host of S. furgiperda. To investigate the internal mechanism of feeding fitness of S. furgiperda to wheat, transcriptomes of S. furgiperda larva feeding on corn, wheat and artificial diet were sequenced and compared. Totally, 66407 unigenes were obtained after assembly and 18868 unigenes were functionally annotated. Respective 542 and 6863 differentially expressed genes were found in wheat-feeding larva and artificial-diet-feeding larva, comparing with corn-feeding larva. Analysis of differentially expressed genes showed that lots of cuticle-related genes up regulated in wheat-feeding larva, while down regulated in artificial-diet-feeding larva. Cytochrome P450 genes were found down regulated in both wheat-feeding and artificial-diet-feeding larva. This study provides information and reference to risk assessment that S. furgiperda damage wheat through overwintering stage.

Key words: Spodoptera furgiperda, transcriptome, feeding, wheat, artificial diet

CLC Number:

S433.4

WANG Siliang, SHAO Yue, YAN Chengjin. Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1236-1247.

Figures/Tables 10

References 31

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样本 Sample	Read总数 Total reads	碱基总数 Total base	GC(%)	Q20(%)	Q30(%)
FAWsl1	23 478 269	7 043 480 700	49.1	97.52	92.99
FAWsl2	23 868 394	7 160 518 200	50.23	97.6	93.16
FAWsl3	23 171 989	6 951 596 700	46.52	97.14	92.16
FAWw1	24 749 086	7 424 725 800	49.76	97.48	92.89
FAWw2	21 784 418	6 535 325 400	48.55	97.48	92.98
FAWw3	25 620 055	7 686 016 500	49.01	97.7	93.41
FAWym1	26 381 427	7 914 428 100	47.86	97.5	92.99
FAWym2	23 284 104	6 985 231 200	47.75	97.47	92.91
FAWym3	22 446 880	6 734 064 000	47.81	97.29	92.56

样本 Sample	Read总数 Total reads	碱基总数 Total base	GC(%)	Q20(%)	Q30(%)
FAWsl1	23 478 269	7 043 480 700	49.1	97.52	92.99
FAWsl2	23 868 394	7 160 518 200	50.23	97.6	93.16
FAWsl3	23 171 989	6 951 596 700	46.52	97.14	92.16
FAWw1	24 749 086	7 424 725 800	49.76	97.48	92.89
FAWw2	21 784 418	6 535 325 400	48.55	97.48	92.98
FAWw3	25 620 055	7 686 016 500	49.01	97.7	93.41
FAWym1	26 381 427	7 914 428 100	47.86	97.5	92.99
FAWym2	23 284 104	6 985 231 200	47.75	97.47	92.91
FAWym3	22 446 880	6 734 064 000	47.81	97.29	92.56

指标Index	数据Data
N50 Length（nt)	1 768
Total Number	66 407
Mean Length	1 063.99
300-500	28 559(43.01%)
500-1 000	19 891(29.95%)
1 000-2 000	9 546(14.37%)
2 000+	8 411(12.67%)

指标Index	数据Data
N50 Length（nt)	1 768
Total Number	66 407
Mean Length	1 063.99
300-500	28 559(43.01%)
500-1 000	19 891(29.95%)
1 000-2 000	9 546(14.37%)
2 000+	8 411(12.67%)

数据库Database	基因总数Number of genes	百分比Percentage	300≤length<1000	length≥1000
COG	4 961	26.29%	736	4 225
GO	10 129	53.68%	2 044	8 085
KEGG	17 615	93.36%	5 902	11 713
KOG	10 271	54.44%	2 068	8 203
Pfam	11 218	59.46%	2 197	9 021
Swissprot	10 910	57.82%	2 215	8 695
NR	18 645	98.82%	6 693	11 952
所有注释Total annotations	18 868		6 867	12 001

Transcriptome analysis of Spodoptera furgiperda during corn-wheat host alternation

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样本 Sample	差异表达基因 Differential expressed genes			有注释的差异表达基因 Annotated differential expressed genes
	总数 Total	上调 Up-regulated	下调 Down-regulated	总数 Total	上调 Up-regulated	下调 Down-regulated
FAWsl	6 863	2 996	3 867	4 607	2 364	2 243
FAWw	542	308	234	432	263	169

样本 Sample	FAWw特异性差异表达基因 Specific differential expressed genes in FAWw	共同差异表达基因 Common differential expressed genes			FAWsl特异性差异表达基因 Specific differential expressed genes in FAWsl
	FAWw特异性差异表达基因 Specific differential expressed genes in FAWw	FAWsl中反方向* Reverse direction in FAWsl	同方向 Same direction	FAWw中反方向 Reverse direction in FAWw
上调 Up-regulated	87	94	82	30	2 131
下调 Down-regulated	41	30	98	94	2 172

基因 gene	上调 Up-regulated				下调 Down-regulated
	总共 Total	特异 Specific	FAWsl中下调down-regulated in FAWsl	共同 Common	总共 Total	特异 Specific	FAWsl中上调 up-regulated in FAWsl	共同 Common
表皮相关基因 Cuticle-related genes	32	5	27	0	3	0	1	2
细胞色素P450基因 Cytochrome P450 genes	3	2	1	0	13	4	1	8

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