Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity

doi:10.3969/j.issn.1004-1524.2020.05.11

›› 2020, Vol. 32 ›› Issue (5): 831-839.DOI: 10.3969/j.issn.1004-1524.2020.05.11

• Plant Protection • Previous Articles Next Articles

Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity

FENG Jiangpeng^1,2, QIU Liping³, LIANG Xiuyan^1,2, CHEN Bixiu^1,2, XIA Haiyang¹, PENG Chunlong², ZHONG Yongjun^1,2,*

1. Institute of Biopharmaceuticals, Taizhou University, Taizhou 318000, China;
2. School of Life Sciences, Taizhou University, Taizhou 318000, China;
3. Biotech Research Institute, Taizhou Academy of Agriculture Sciences, Linhai 317000, China

Received:2019-12-10 Online:2020-05-25 Published:2020-05-29

Abstract

Abstract: A bacterial isolate JK3, which was isolated from compost, showed stronger antagonistic activity against Colletotrichum gloeosporioides causing strawberry anthracnose in dual culture assay. The isolate JK3 was identified as Bacillus velezensis by physiological and biochemical characterization, 16S rDNA and rpoB gene sequence analysis. The inhibitory effects of JK3 fermented liquid on C. gloeosporioides were evaluated with the detached strawberry leaves. JK3 fermented liquid showed significant protection on strawberry anthracnose but poor therapeutic effect. More than 95% control efficiency was achieved by spraying JK3 fermented liquid 24 h before inoculation with the spore suspension of C. gloeosporioides or simultaneous inoculation. Only 11.5% control efficiency was resulted by spraying JK3 fermented liquid 24 h after inoculation with the pathogen. The antagonistic activity of the fermented liquid was stable to different pH values and proteases, but sensitive to high temperature. Thus implied that active component was not related to proteins. It has been reported that most isolates of Bacillus spp. produce active lipopeptides. The existences of lipopeptide biosynthetic genes in JK3 were examined by PCR amplification with proper primer pairs and DNA sequencing. Results showed that the JK3 contained srfAA, bymB, fenD, bacA and ituC genes. The above data indicated that B. velezensis JK3 had a potential to be developed as biocontrol agent for controlling strawberry anthracnose caused by C. gloeosporioides.

Key words: strawberry anthracnose, Colletotrichum gloeosporioides, Bacillus velezensis, biocontrol, antagonistic activity

CLC Number:

S476⁺.1

FENG Jiangpeng, QIU Liping, LIANG Xiuyan, CHEN Bixiu, XIA Haiyang, PENG Chunlong, ZHONG Yongjun. Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity[J]. , 2020, 32(5): 831-839.

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Identification of antagonistic bacteria Bacillus velezensis JK3 against anthracnose of strawberry and its antipathogenic activity

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