Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain

doi:10.3969/j.issn.1004-1524.20231149

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1094-1101.DOI: 10.3969/j.issn.1004-1524.20231149

• Plant Protection • Previous Articles Next Articles

Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain

HE Yushan¹^,²^,³(), ZHU Haixia¹^,²^,³^,^*()

1. Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China
2. Xining Crop Pest Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Xining 810016, China
3. Key Laboratory of Comprehensive Management of Agricultural Pests of Qinghai Province, Xining 810016, China

Received:2023-09-26 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

Recently, microbial herbicides have become a research hotspot due to their advantages in safety and environment protection. In order to explore the weeding mechanism of Alternaria gaisen GD-011 strain, experiments were carried out on Elsholtzia densa Benth by observing the ultrastructure changes of E. densa leaves after inoculation of GD-011 strain, as well as determining the changes of physiological and biochemical indexes of leaves. The results showed that the mycelia of strain GD-011 invaded E.densa through stomata to leaf tissue, parasitically reproduced in the tissue, produced spores, and gradually destroyed the tissue. After inoculation of strain GD-011, the malondialdehyde (MDA) content in the leaves of E. densa gradually increased. Meanwhile, the activities of antioxidant enzyme systems, such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), increased first and then decreased. The soluble protein content in the leaves of E. densa after inoculation for 2-7 d was significantly (P<0.05) higher than that in the control. In general, it was inferred that the GD-011 strain achieved weeding effect by infecting through stomata, destroying the cell membrane and tissue structure and inhibiting self-repair, etc.

Key words: microbial herbicides, physiology and biochemistry, ultrastructure, weeding mechanism

CLC Number:

S476.1

HE Yushan, ZHU Haixia. Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1094-1101.

Figures/Tables 6

Fig.1 Characteristics of Elsholtzia densa leaf tissue after inoculation of strain GD-011 for 0-7 d (A-H accordingly) by scanning electron microscope observation

Fig.2 Malondialdehyde content of Elsholtzia densa leaves under treatments Dots marked without the same letters indicate significant difference at different time under the same treatment at P<0.05. * indicates significant difference between the CK group and the treatment group at P<0.05.The same as below.

Fig.3 Catalase activity of Elsholtzia densa leaves under treatments

Fig.4 Peroxidase activity of Elsholtzia densa leaves under treatments

Fig.5 Superoxide dismutase activity of Elsholtzia densa leaves under treatments

Fig.6 Soluble protein content of Elsholtzia densa leaves under treatments

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Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain

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