Isolation, identification and biocontrol mechanism of antagonistic fungus against Chinese pepper gummosis

doi:10.3969/j.issn.1004-1524.20230077

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (2): 373-382.DOI: 10.3969/j.issn.1004-1524.20230077

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Isolation, identification and biocontrol mechanism of antagonistic fungus against Chinese pepper gummosis

ZHAO Jitao(), HE Jing^*(), DING Dedong, LI Yanxiang, HOU Caixia, ZHAO Qian

College of Forestry; b. Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Gansu Agricultural University, Lanzhou 730070, China

Received:2023-01-18 Online:2024-02-25 Published:2024-03-05

Abstract

Abstract:

Chinese pepper gummosis caused by Fusarium tricinctum is a serious disease of Zanthoxylum bungeanum in recent years. In order to obtain the excellent antagonistic strain of F. tricinctum, and the endophytic fungi with strong antagonistic effect were screened and isolated by plate confrontation method. A strain with high antagonistic activity was obtained, and its inhibition rate reached 58.85%, which was named HJ-18. Based on morphological and ITS molecular identification, it was preliminarily identified as Schizophyllum commune (GenBank accession number: OP502072). The antifungal spectrum test showed that it had inhibitory effect on 4 plant pathogenic fungi. After treatment with different light, temperature and pH, the antifungal effect was stable, but sensitive to ultraviolet ray. When the volume fraction of culture filtrate was 50%, the inhibition rate of mycelium growth of F. tricinctum was the highest, reaching 66.40%. Scanning electron microscope observation showed that after treatment with culture filtrate, the mycelium of F. tricinctum was wrinkled, and debris was produced on the surface. The results of pot experiment showed that the relative prevention effect of strain HJ-18 was 52.65%, which was the same as 100-fold dilution of 22.5% picoxystrobin treatment. The above results show that strain HJ-18 is an endophytic fungus with biocontrol potential and can be used as a biocontrol agent.

Key words: Chinese pepper gummosis, endophytic, antagonistic strains, culture filtrate, biocontrol mechanism

CLC Number:

S435.73

ZHAO Jitao, HE Jing, DING Dedong, LI Yanxiang, HOU Caixia, ZHAO Qian. Isolation, identification and biocontrol mechanism of antagonistic fungus against Chinese pepper gummosis[J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 373-382.

Figures/Tables 9

Fig.1 Inhibitory effect of strain HJ-18 on Fusarium tricinctum A, Control (front); B, Control (reverse); C, Confrontation effect (front); D, Confrontation effect (reverse). Culture time was 7 days.

Fig.2 Morphological characteristics of strain HJ-18 A, Colony morphology (front); B, Colony morphology (reverse); C, Mycelium; D, Spore.

Fig.3 The phylogenetic tree of strain HJ-18 based on ITS sequence

Fig.4 Inhibitory effect of strain HJ-18 on several plant pathogenic fungi The bars marked without the same lowercase letter indicated significant differences at P<0.05. The same as below.

Fig.5 Stability of the culture filtrate of strain HJ-18 under different conditions

Fig.6 Effects of culture filtrate of strain HJ-18 against the mycelial growth of Fusarium tricinctum A, Colony diameter; B, Inhibition ratio.

Fig.7 Effect of culture filtrate of strain HJ-18 on mycelial morphology of Fusarium tricinctum A, Control (×1 000); B, Control (×4 000); C, Treated by HJ-18 culture filtrate (×1 000); D, Treated by HJ-18 culture filtrate (×4 000).

Fig.8 Morphology of Zanthoxylum bungeanum seedlings and stems under different treatments A, Inoculation with Fusarium tricinctum; B, Fusarium tricinctum+HJ-18 treatment; C, 100-fold dilution of 22.5% picoxystrobin treatment; D, Sterile water treatment; E, Inoculation with strain HJ-18 before the Fusarium tricinctum.

Table 1 Control effect of strain HJ-18 on Chinese pepper gummosis

处理 Treatment	病情指数 Disease index	相对防效 Relative control effect/%
病原菌F. tricinctum	77.78±2.22 a
病原菌+菌株HJ-18	36.67±3.33 c	52.65±5.10 a
F. tricinctum+HJ-18
病原菌+啶氧菌酯	40.00±0.00 c	48.48±1.52 a
F. tricinctum+ picoxystrobin
病原菌+无菌水	63.33±3.33 b	18.56±3.61 b
F. tricinctum+sterile water
菌株HJ-18+病原菌	33.33±6.67 c	56.82±9.19 a
HJ-18+F. tricinctum

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Isolation, identification and biocontrol mechanism of antagonistic fungus against Chinese pepper gummosis

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