Antifungal activities of Bacillus subtilis STO-12 and analysis on its antifungal substances

doi:10.3969/j.issn.1004-1524.2017.01.12

›› 2017, Vol. 29 ›› Issue (1): 81-88.DOI: 10.3969/j.issn.1004-1524.2017.01.12

• Plant Protection • Previous Articles Next Articles

Antifungal activities of Bacillus subtilis STO-12 and analysis on its antifungal substances

HUANG Huayi¹, WANG Jialin², MA Rong², LIANG Yingmei³, TIAN Chengming^{1, *}

1. Key Laboratory for Silviculture and Conservation of Ministry of Education College of Forestry, Beijing Forestry University, Beijing 100083, China;
2. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
3. Museum of Beijing Forestry University, Beijing Forestry University, Beijing 100083, China

Received:2016-05-11 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: Bacillus subtilis STO-12 was isolated from the rhizosphere soil of jujube plants as a biocontrol agent. In order to provide technical guidance for further exploitation and utilization of STO-12, the antifungal activities of STO-12 and its sterile culture filtrates were investigated by dual culture assay and colony diameter assay, respectively; and the antifungal substances of STO-12 were analyzed by paper disc method and two-compartment plate method, respectively. The results revealed that STO-12 and its sterile culture filtrates had strong antifungal activities, and the rates of sterile culture filtrates inhibited Alternaria tenuissima, Colletotrichum gloeosporioides and Cytospora chrysosperma were 84.87% (7 d), 38.19% (7 d) and 100% (3 d), respectively. Crude proteins and lipopeptides extracted from STO-12 exhibited high antifungal activities against Alternaria tenuissima, Colletotrichum gloeosporioides and Cytospora chrysosperma and the hyphae at the edge of inhibition zones developed swelling. For crude proteins, the average diameters of the inhibition zones for Alternaria tenuissima, Colletotrichum gloeosporioides and Cytospora chrysosperma were 12.33, 9.50 and 16.50 mm, respectively. For lipopeptides, the average diameters of the inhibition zones were 17.50, 17.06 and 21.50 mm, respectively. STO-12 could produce cellulase and protease. Moreover, volatiles produced by STO-12 also could inhibit the hyphal growth of Alternaria tenuissima, Colletotrichum gloeosporioides and Cytospora chrysosperma, and the inhibition rates were 38.96% (3 d), 9.35% (3 d) and 82.14% (2 d), respectively. Our results suggested that Bacillus subtilis STO-12 had strong antifungal activity, varied antifungal substances and wide antifungal spectrum, had the potential prospect of industrial development and field application.

Key words: Bacillus subtilis, antifungal activity, culture filtrate, antifungal proteins, lipopeptides

CLC Number:

S476

HUANG Huayi, WANG Jialin, MA Rong, LIANG Yingmei, TIAN Chengming. Antifungal activities of Bacillus subtilis STO-12 and analysis on its antifungal substances[J]. , 2017, 29(1): 81-88.

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Antifungal activities of Bacillus subtilis STO-12 and analysis on its antifungal substances

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