Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques

doi:10.3969/j.issn.1004-1524.2017.01.13

›› 2017, Vol. 29 ›› Issue (1): 89-100.DOI: 10.3969/j.issn.1004-1524.2017.01.13

• Plant Protection • Previous Articles Next Articles

Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques

YIN Minghua¹, Zhou Yuyao², YANG Xingpeng¹, XU Yuqin¹, LIU Zhengying¹, SHU Rongjian¹, WEI Zhimin¹, Xia Huayan¹, Hong Senrong^{1, *}

1. College of Life Sciences, Shangrao Normal University, Shangrao 334001, China;
2. College of Humanities, Beijing University of Chinese Medicine, Beijing 100029, China

Received:2016-05-05 Online:2017-01-15 Published:2017-02-23

Abstract

Abstract: Virus species in 3 main cultivars of early pear in Shangrao were analyzed and the efficiency of their shoot tip virus-free techniques was compared. The results showed that Apple stem pitting virus (ASPV) was not detected in Huating Liuyuexue and Tiandun Liuyuexue, but it was detected in Huating Huangpixiao. Apple stem grooving virus(ASGV) and Apple scar skin viroid(ASSVd) were both detected in Huating Liuyuexue, Huating Huangpixiao and Tiandun Liuyuexue, but Apple chlorotic leaf spot virus(ACLSV) and Apple mosaic virus (ApMV) were not detected in them. For 3 main cultivars of early pear in Shangrao, the virus-free efficiency of cryotherapy was significantly higher than that of the conventional shoot tip culture. Among three virus-free techniques by cryotherapy, the virus-free efficiency of cryotherapy by encapsulation-vitrification was the highest. Through SSR fluorescence labeled capillary electrophoresis analysis, the conventional shoot tip culture and three cryotherapy virus-free techniques all could ensure the genetic stability of 3 main cultivars of early pear in Shangrao. The results contributed to the viral disease detection, identification and their real-time monitoring and quarantine of early pear seedling in Shangrao, which also provided some technical support for building the cryopreservation germplasm resources of early pear in Shangrao and its production of virus-free plantlets.

Key words: early pear in Shangrao, main cultivars, virus species, shoot tip, cryotherapy, virus-free

CLC Number:

S661.2

YIN Minghua, Zhou Yuyao, YANG Xingpeng, XU Yuqin, LIU Zhengying, SHU Rongjian, WEI Zhimin, Xia Huayan, Hong Senrong. Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques[J]. , 2017, 29(1): 89-100.

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Analysis of virus species in main cultivars of early pear in Shangrao and the efficiency of their shoot tip virus-free techniques

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