Abstract: The whole cDNA of E gene was amplified by PCR from duck Tembusu virus (DTMUV) strain BZ, and cloned into the pMD18-T vector. The fragment was identified by restriction enzymes digestion with EcoRⅠ and XhoⅠ, and then was cloned into the pET-28a (+) vector. The recombinant expression plasmid PET28a-E was obtained and transformed into BL21 (DE3). After the recombinant bacteria were induced by optimal concentration of IPTG, the E fusion protein was proved to get exact expression and have good immunogenicity by SDS-PAGE and Western blotting. Base on the expressed protein an indirect ELISA was established to detect DTMUV antibody in duck serum. The indirect ELISA shared 95.0% coincidence rate with the neutralization test, which showed that this method had a good prospect on the detection of DTMUV antibody．

Key words: duck Tembusu virus (DTMUV), E gene, expression, indirect ELISA