Expression abundance analysis of the V-ATPase subunit A gene in Plutella xylostella

doi:10.3969/j.issn.1004-1524.20240285

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (4): 839-846.DOI: 10.3969/j.issn.1004-1524.20240285

• Plant Protection • Previous Articles Next Articles

Expression abundance analysis of the V-ATPase subunit A gene in Plutella xylostella

LU Lina¹^,²(), ZHU Qing¹^,², GAO Meijing¹^,², XIE Yajing¹^,², LIU Xianjin¹^,², ZHANG Zhiyong¹^,²^,^*()

1. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
2. Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Received:2024-03-28 Online:2025-04-25 Published:2025-05-09

Abstract

Abstract:

V-ATPase plays important role in insects and mechanism of Cry toxins. In this study, the molecular cloning and real time fluorogenic quantitative PCR(qRT-PCR) was conducted to explore the expression of Plutella xylostella V-ATPase subunit A gene in different growth and developmental stages as well as before/after feeding Cry1Ac toxin. The results showed that the CDS of V-ATPase subunit A gene was 1 848 bp, which encoded 615 amino acids (GenBank No. ON108624) and had approximately 94% homology with V-ATPase subunit A of Ostrinia furnacalis, Chilo suppressalis and Helicoverpa armigera. The qRT-PCR results indicated that V-ATPase subunit A gene was expressed in the whole larval stages, eggs, pupa and adults. In eggs and pupa, the expression level of V-ATPase subunit A gene was low, while in the 2nd instar larvae the expression reached the highest to be 4.16 times as in eggs, and decreased in the 3rd and 4th instar larvae. After feeding the artificial diet with Cry1Ac toxins, the express level of V-ATPase subunit A gene declined and then reached to the normal after feeding 48 h. These results indicated that the express level of V-ATPase subunit A gene in P. xylostella was closely related with development and metabolic changes, and might be involved in the mode action of Cry1Ac toxin.

Key words: diamondback moth (Plutella xylostella), V-ATPase subunit A, Cry toxin, expression level

CLC Number:

S433.4

LU Lina, ZHU Qing, GAO Meijing, XIE Yajing, LIU Xianjin, ZHANG Zhiyong. Expression abundance analysis of the V-ATPase subunit A gene in Plutella xylostella[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 839-846.

Figures/Tables 5

Fig.1 Amino acid sequence alignment of V-ATPase subunit A

Fig.2 Homology analysis of V-ATPase subunit A in different insects

Fig.3 The expression level of V-ATPase subunit A gene at different developmental stages of Plutella xylostella Data marked without the same lowercase letters indicate significant difference(P<0.05). The same as below.

Fig.4 Mortality of Plutella xylostella larvae treated with different mass concentrations of Cry1Ac toxin

Fig.5 The expression level of V-ATPase subunit A gene in Plutella xylostella larvae after feeding Cry1Ac toxin

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Expression abundance analysis of the V-ATPase subunit A gene in Plutella xylostella

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